AIM To reveal the inhibitory effects ofCurcuma aromatica oil(CAO)on cellproliferation of hepatoma in mice.METHODS Two tumor inhibitory experimentsof CAO on hepatoma in mice were conducted.The inhibitory effects of CAO...AIM To reveal the inhibitory effects ofCurcuma aromatica oil(CAO)on cellproliferation of hepatoma in mice.METHODS Two tumor inhibitory experimentsof CAO on hepatoma in mice were conducted.The inhibitory effects of CAO on proliferation ofhepatoma in mice were evaluated by DNA imagecytometry and immunohistochemical staining ofproliferating cell nuclear antigen(PCNA).RESULTS The tumor inhibitory rates of CAOwere 52% and 51% in two experiments,respectively.Compared with those of the saline-treated control groups,both differences werestatistically significant(P【0.01).In the groupof mice treated with CAO,the cellular nuclearDNA OD value(249±70),areas(623 μm^2±228 μm^2)and DNA(2.38±0.67)index of hepaticcarcinomas were significantly lower than thoseof the control group(430±160,1073 μm^2±101 μm^2 and 4.48±0.71).CAO also couldincrease diploidy cell rates(29.00%±9.34% vs2.97%±5.69%,P【0.01)and decreasepentaploidy cell exceeding rate(30.04%±15.10% vs 70.89%±14.94%,P【0.01).In thegroup of mice treated with CAO,the labelingindexes of proliferating cell nuclear antigen (PCNA-LI)were 30%±4%,which weresignificantly lower than 40%±6% of the controlgroup(P【0.01).CONCLUSION The inhibition of CAO on thegrowth of hepatoma in mice might be associatedwith its depression on cellular proliferativeactivity.展开更多
本文在提取中药郁金中姜黄素类化合物的基础上,利用毛细管区带电泳法对郁金中所含黄素类化合物进行了分离研究,确定了最佳分离条件:9mmol/L单-6-O-苯基氨甲酰基-β-CD(mono-6-O-phenylcarbamoyl-β-CD),40mmol/L硼砂缓冲液,分离电压为15...本文在提取中药郁金中姜黄素类化合物的基础上,利用毛细管区带电泳法对郁金中所含黄素类化合物进行了分离研究,确定了最佳分离条件:9mmol/L单-6-O-苯基氨甲酰基-β-CD(mono-6-O-phenylcarbamoyl-β-CD),40mmol/L硼砂缓冲液,分离电压为15kV,毛细管柱温度为22℃,紫外检测波长为214nm,pH值为9.0。双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素在此条件下10 m in内能够达到基线分离,方法快速、简便、且消耗试剂少,不污染环境。展开更多
基金National key project of the 9~(th) 5-year Plan for Medicine and Health,No.96-906-07-04Guangdong provincial natural scientific grants,No.980663.
文摘AIM To reveal the inhibitory effects ofCurcuma aromatica oil(CAO)on cellproliferation of hepatoma in mice.METHODS Two tumor inhibitory experimentsof CAO on hepatoma in mice were conducted.The inhibitory effects of CAO on proliferation ofhepatoma in mice were evaluated by DNA imagecytometry and immunohistochemical staining ofproliferating cell nuclear antigen(PCNA).RESULTS The tumor inhibitory rates of CAOwere 52% and 51% in two experiments,respectively.Compared with those of the saline-treated control groups,both differences werestatistically significant(P【0.01).In the groupof mice treated with CAO,the cellular nuclearDNA OD value(249±70),areas(623 μm^2±228 μm^2)and DNA(2.38±0.67)index of hepaticcarcinomas were significantly lower than thoseof the control group(430±160,1073 μm^2±101 μm^2 and 4.48±0.71).CAO also couldincrease diploidy cell rates(29.00%±9.34% vs2.97%±5.69%,P【0.01)and decreasepentaploidy cell exceeding rate(30.04%±15.10% vs 70.89%±14.94%,P【0.01).In thegroup of mice treated with CAO,the labelingindexes of proliferating cell nuclear antigen (PCNA-LI)were 30%±4%,which weresignificantly lower than 40%±6% of the controlgroup(P【0.01).CONCLUSION The inhibition of CAO on thegrowth of hepatoma in mice might be associatedwith its depression on cellular proliferativeactivity.
文摘本文在提取中药郁金中姜黄素类化合物的基础上,利用毛细管区带电泳法对郁金中所含黄素类化合物进行了分离研究,确定了最佳分离条件:9mmol/L单-6-O-苯基氨甲酰基-β-CD(mono-6-O-phenylcarbamoyl-β-CD),40mmol/L硼砂缓冲液,分离电压为15kV,毛细管柱温度为22℃,紫外检测波长为214nm,pH值为9.0。双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素在此条件下10 m in内能够达到基线分离,方法快速、简便、且消耗试剂少,不污染环境。
