Azoxystrobin acts as an inhibitor of electron transport by binding to the Qo center of cytochrome b (cyt b). Resistance to azoxystrobin was usually caused by the point mutation of cyt b gene or by the induction of alt...Azoxystrobin acts as an inhibitor of electron transport by binding to the Qo center of cytochrome b (cyt b). Resistance to azoxystrobin was usually caused by the point mutation of cyt b gene or by the induction of alternative respiration. Oxygen consumption test for mycelia of Colletotrichum capsici showed that azoxystrobin inhibited mycelial respiration within 12 h; however, as time went on, the respiration of the mycelia recovered when the mycelia were treated with azoxystrobin and salicylhydroxamic acid (SHAM, a known inhibitor of alternative respiration), and the oxygen consumption of the mycelia could not be inhibited. Meanwhile, cytochrome b (cyt b) gene expression increased with the recovery of mycelial respiration. The increased cyt b gene expression might play a role in the development of resistance to azoxystrobin in C. capsici.展开更多
为了进一步探讨大麝鼩(Crocidura lasiura)的亚种分化问题,在中国东北地区采集大麝鼩标本13号,结合已报道的大麝鼩形态学数据和Cyt b基因序列一并进行分析。形态学结果显示:在绝大多数个体中,分布于中国东北—俄罗斯远东的大麝鼩体型大...为了进一步探讨大麝鼩(Crocidura lasiura)的亚种分化问题,在中国东北地区采集大麝鼩标本13号,结合已报道的大麝鼩形态学数据和Cyt b基因序列一并进行分析。形态学结果显示:在绝大多数个体中,分布于中国东北—俄罗斯远东的大麝鼩体型大于分布于韩国的个体(体长:> 82 mm vs.<82 mm,尾长:> 39 mm vs.<39 mm,颅基长:> 20 mm vs.<20 mm);中国东北—俄罗斯远东种群体背毛色更黑,韩国种群体背毛色更灰;中国东部种群尾较长,绝大多数超过体长之半;而分布于中国东北—俄罗斯远东、韩国的大麝鼩种群尾长绝大多数不及体长之半。基于Cyt b基因的遗传距离、系统发育关系和中介网络分析,都显示大麝鼩样本可分为3个地理谱系:中国东北—俄罗斯远东谱系、韩国谱系、中国东部谱系。研究结果支持大麝鼩分为3个亚种:东北亚种(C. l. lasiura)分布于中国东北和俄罗斯远东,韩国亚种(C. l. thomasi)分布于韩国,华东亚种(C. l. campus-lincolnensis)分布于中国东部。展开更多
Objective To investigate the effects of the B7-H4 gene rs10754339 and miR-125a gene rs12976445 on cancer susceptibility through a case-control study and meta-analysis.Methods A total of 1,490 cancer patients(lung/gast...Objective To investigate the effects of the B7-H4 gene rs10754339 and miR-125a gene rs12976445 on cancer susceptibility through a case-control study and meta-analysis.Methods A total of 1,490 cancer patients(lung/gastric/liver/:550/460/480)and 800 controls were recruited in this case-control study.The meta-analysis was performed by pooling the data from previous related studies and the present study.Results The results of this study showed that in the Hubei Han Chinese population,the rs10754339gene was significantly associated with the risk of lung and gastric cancer but not liver cancer,and the rs12976445 gene was significantly associated with the risk of lung cancer but not liver or gastric cancer.The meta-analysis results indicated that rs10754339 and rs12976445 contributed to cancer susceptibility in the Chinese population and also revealed a significant association between rs10754339and breast cancer risk,as well as between rs12976445 and lung cancer risk.Conclusion The B7-H4 gene rs10754339 and miR-125a gene rs12976445 may be the potential genetic markers for cancer susceptibility in the Chinese population,which should be validated in future studies with larger sample sizes in other ethnic populations.展开更多
基金sponsored by the National 973 Program of China (2006CB101907)the National 863 Program of China (2006AA10A211, 2008AA10Z414)the National Natural Science Foundation of China(30671048, 30671384)
文摘Azoxystrobin acts as an inhibitor of electron transport by binding to the Qo center of cytochrome b (cyt b). Resistance to azoxystrobin was usually caused by the point mutation of cyt b gene or by the induction of alternative respiration. Oxygen consumption test for mycelia of Colletotrichum capsici showed that azoxystrobin inhibited mycelial respiration within 12 h; however, as time went on, the respiration of the mycelia recovered when the mycelia were treated with azoxystrobin and salicylhydroxamic acid (SHAM, a known inhibitor of alternative respiration), and the oxygen consumption of the mycelia could not be inhibited. Meanwhile, cytochrome b (cyt b) gene expression increased with the recovery of mycelial respiration. The increased cyt b gene expression might play a role in the development of resistance to azoxystrobin in C. capsici.
文摘为了进一步探讨大麝鼩(Crocidura lasiura)的亚种分化问题,在中国东北地区采集大麝鼩标本13号,结合已报道的大麝鼩形态学数据和Cyt b基因序列一并进行分析。形态学结果显示:在绝大多数个体中,分布于中国东北—俄罗斯远东的大麝鼩体型大于分布于韩国的个体(体长:> 82 mm vs.<82 mm,尾长:> 39 mm vs.<39 mm,颅基长:> 20 mm vs.<20 mm);中国东北—俄罗斯远东种群体背毛色更黑,韩国种群体背毛色更灰;中国东部种群尾较长,绝大多数超过体长之半;而分布于中国东北—俄罗斯远东、韩国的大麝鼩种群尾长绝大多数不及体长之半。基于Cyt b基因的遗传距离、系统发育关系和中介网络分析,都显示大麝鼩样本可分为3个地理谱系:中国东北—俄罗斯远东谱系、韩国谱系、中国东部谱系。研究结果支持大麝鼩分为3个亚种:东北亚种(C. l. lasiura)分布于中国东北和俄罗斯远东,韩国亚种(C. l. thomasi)分布于韩国,华东亚种(C. l. campus-lincolnensis)分布于中国东部。
基金supported by the Fundamental Research Funds for the Central Universities (WUT:2020IB029)。
文摘Objective To investigate the effects of the B7-H4 gene rs10754339 and miR-125a gene rs12976445 on cancer susceptibility through a case-control study and meta-analysis.Methods A total of 1,490 cancer patients(lung/gastric/liver/:550/460/480)and 800 controls were recruited in this case-control study.The meta-analysis was performed by pooling the data from previous related studies and the present study.Results The results of this study showed that in the Hubei Han Chinese population,the rs10754339gene was significantly associated with the risk of lung and gastric cancer but not liver cancer,and the rs12976445 gene was significantly associated with the risk of lung cancer but not liver or gastric cancer.The meta-analysis results indicated that rs10754339 and rs12976445 contributed to cancer susceptibility in the Chinese population and also revealed a significant association between rs10754339and breast cancer risk,as well as between rs12976445 and lung cancer risk.Conclusion The B7-H4 gene rs10754339 and miR-125a gene rs12976445 may be the potential genetic markers for cancer susceptibility in the Chinese population,which should be validated in future studies with larger sample sizes in other ethnic populations.
