Metabolites derived from marine symbiotic microorganisms have great potential as lead compounds for the discovery of novel marine drugs. Euphausia superba Dana, which lives in the Antarctic Ocean, is regarded as a new...Metabolites derived from marine symbiotic microorganisms have great potential as lead compounds for the discovery of novel marine drugs. Euphausia superba Dana, which lives in the Antarctic Ocean, is regarded as a new source of marine microbial natural products. However, no studies have examined the biodiversity of the symbiotic intestinal microbiome of E. superba. To address this issue, the species diversity and abundance of the gut microbiome of E. superba Dana from the Rose Sea of the Antarctic Ocean were analyzed by culture-independent high-throughput sequencing and pure culture methods. A comparison with gene databases revealed that the microbiome contained 61 known microbial species and a plethora of uncultivable microorganisms. Additionally, 7% of the species in the microbiome were currently unknown. The microbes belonged to 56 genera, eight of which, including Arthrobacter, Bacillus, Candidatus, Lactococcus, Lysinibacillus, Leuconostoc, Solibacillus, and Vibrio, were dominant, as were Vibrionaceae spp. Moreover, 81 microbial strains were isolated by the pure culture method, and they belonged to 36 genera, including Mobilicoccus, Rhodococcus, Arthrobacter, and Microbacterium. The results obtained by two different methods demonstrate the richness of the microbial biodiversity of the gut microbiome of E. superba, and it also suggests that they have good potential for the discovery of novel marine microbial species.展开更多
Seasonal abundance of the marine cladoceran Penilia avirostris was studied in the Tolo Harbour,Hong Kong,from November 1987 to December 1989.P.avirostris occurred in plankton throughout the year,but no clear seasonal ...Seasonal abundance of the marine cladoceran Penilia avirostris was studied in the Tolo Harbour,Hong Kong,from November 1987 to December 1989.P.avirostris occurred in plankton throughout the year,but no clear seasonal pattern could be identified. In general,densities were the highest in the Tolo Channel and decreased progressively towards the inner parts of the Tolo Harbour.P.avirostris showed marked seasonal variations in body size and brood size. Body length of P. avirostris ranged from 0.3 to 1.0mm. Average body length was greatest on March 1988 and January 1989, and the smallest in May 1988, although no clear seasonal trends could be detected. Brood size of P. avirostris ranged from 1 to 13 over the study period with a mean of 5.6.A significant positive relationship was found between body length and brood size. Diel vertical migration and diel feeding pattern of P. avirostris was followed over a 24 h period from July 31 to August 1, 1991 at a station near the southern edge of the dam of the Plover Cove Reservoir. P.avirostris Performed typical nocturnal vertical migration. Although animals were seldom found below 10 m during either day or night,a greater fraction of the population was found in the upper 1 to 3 m at night than during the day.A diel cycle in gut pigment content was observed in P.avirostris. Gut pigment content was low during the day and increased sharply during the first 2 h after sunset.Gut pigment content at 21:00 and 03:00 was about 2 times higher than average daytime values.Chlorophyll a concentration was the highest near the surface and de creased towards the bottom, but water collected at 16 m still contained 1.79 ng/cm3 Chl.a. Because P. avirostris were usually found within the upper 10 m,we believe that the observed diel change in gut pigment content was like ly the result of a real diel feeding rhythm, and was probably not the result of individual animals moving in and out of the food-rich layer.展开更多
Sampling date and position are shown in Table 1. Complete E. superbasamples with fullstomach were usua1ly used. They were averagely 45 mm long and 1.5 g weight (wet). Beingconcentrated with filtered sea water, every f...Sampling date and position are shown in Table 1. Complete E. superbasamples with fullstomach were usua1ly used. They were averagely 45 mm long and 1.5 g weight (wet). Beingconcentrated with filtered sea water, every food species from krill stomach was observed andidentified under an optical microscope, and their size was measured. Food species in stomachcontent of E. superbawere divided into five classes, according to the presence of species indi-展开更多
Outlook 2009年会于2009年9月23—25日在马耳他首都瓦莱塔的Grand Excelsior酒店举行。会议吸引了来自用即弃产品供应链各个环节的近400名代表参加。这些代表听取了业界专家所做的题材广泛的演讲。该年会反映了非织造布行业现状,会议...Outlook 2009年会于2009年9月23—25日在马耳他首都瓦莱塔的Grand Excelsior酒店举行。会议吸引了来自用即弃产品供应链各个环节的近400名代表参加。这些代表听取了业界专家所做的题材广泛的演讲。该年会反映了非织造布行业现状,会议内容主要集中在可持续发展和环境友好两大话题,一些绿色环保运动的顶级专家就减少非织造布碳足迹的方法做了专题演讲。另外一些涵盖了无线射频自动识别电子标签技术(RFID)、原材料价格、经济危机和用即弃产品的性能参数的议题,也成为亮点。展开更多
基金funds from national non-profit institutes (East China Sea Fisheries Research Institute) (Grant no.2014M02)the Opening Fund Project (Grant no.KP201406) of the State Oceanic Administration Key Laboratory for Polar Science at the Polar Research Institute of China+1 种基金the Chinese Polar Environment Comprehensive Investigation & Assessment Program (Grant no.CHINARE2016-01-05)the National High-tech R&D Program of China (Program 863, Grant no.2012AA092105)
文摘Metabolites derived from marine symbiotic microorganisms have great potential as lead compounds for the discovery of novel marine drugs. Euphausia superba Dana, which lives in the Antarctic Ocean, is regarded as a new source of marine microbial natural products. However, no studies have examined the biodiversity of the symbiotic intestinal microbiome of E. superba. To address this issue, the species diversity and abundance of the gut microbiome of E. superba Dana from the Rose Sea of the Antarctic Ocean were analyzed by culture-independent high-throughput sequencing and pure culture methods. A comparison with gene databases revealed that the microbiome contained 61 known microbial species and a plethora of uncultivable microorganisms. Additionally, 7% of the species in the microbiome were currently unknown. The microbes belonged to 56 genera, eight of which, including Arthrobacter, Bacillus, Candidatus, Lactococcus, Lysinibacillus, Leuconostoc, Solibacillus, and Vibrio, were dominant, as were Vibrionaceae spp. Moreover, 81 microbial strains were isolated by the pure culture method, and they belonged to 36 genera, including Mobilicoccus, Rhodococcus, Arthrobacter, and Microbacterium. The results obtained by two different methods demonstrate the richness of the microbial biodiversity of the gut microbiome of E. superba, and it also suggests that they have good potential for the discovery of novel marine microbial species.
文摘Seasonal abundance of the marine cladoceran Penilia avirostris was studied in the Tolo Harbour,Hong Kong,from November 1987 to December 1989.P.avirostris occurred in plankton throughout the year,but no clear seasonal pattern could be identified. In general,densities were the highest in the Tolo Channel and decreased progressively towards the inner parts of the Tolo Harbour.P.avirostris showed marked seasonal variations in body size and brood size. Body length of P. avirostris ranged from 0.3 to 1.0mm. Average body length was greatest on March 1988 and January 1989, and the smallest in May 1988, although no clear seasonal trends could be detected. Brood size of P. avirostris ranged from 1 to 13 over the study period with a mean of 5.6.A significant positive relationship was found between body length and brood size. Diel vertical migration and diel feeding pattern of P. avirostris was followed over a 24 h period from July 31 to August 1, 1991 at a station near the southern edge of the dam of the Plover Cove Reservoir. P.avirostris Performed typical nocturnal vertical migration. Although animals were seldom found below 10 m during either day or night,a greater fraction of the population was found in the upper 1 to 3 m at night than during the day.A diel cycle in gut pigment content was observed in P.avirostris. Gut pigment content was low during the day and increased sharply during the first 2 h after sunset.Gut pigment content at 21:00 and 03:00 was about 2 times higher than average daytime values.Chlorophyll a concentration was the highest near the surface and de creased towards the bottom, but water collected at 16 m still contained 1.79 ng/cm3 Chl.a. Because P. avirostris were usually found within the upper 10 m,we believe that the observed diel change in gut pigment content was like ly the result of a real diel feeding rhythm, and was probably not the result of individual animals moving in and out of the food-rich layer.
文摘Sampling date and position are shown in Table 1. Complete E. superbasamples with fullstomach were usua1ly used. They were averagely 45 mm long and 1.5 g weight (wet). Beingconcentrated with filtered sea water, every food species from krill stomach was observed andidentified under an optical microscope, and their size was measured. Food species in stomachcontent of E. superbawere divided into five classes, according to the presence of species indi-