Accompanying with the increasingly saturated genome figures,DNA chip has been widely applied.Thanks to its advantages of integration,miniaturization and automation,DNA chip becomes a powerful research tool in various ...Accompanying with the increasingly saturated genome figures,DNA chip has been widely applied.Thanks to its advantages of integration,miniaturization and automation,DNA chip becomes a powerful research tool in various research fields including biology,medicine and chemistry.This article overviews the application of DNA chip technology in animal medicine from gene expression spectrum research,pathogenic microbial detection,bacterial typing,genetic mutations and polymorphism detection,pathogenic microbial genomics research,as well as its principle and classification.展开更多
The phosphatidylinositol (PI) metabolic pathway is considered critical in plant responses to many environmental factors,and previous studies have indicated the involvement of multiple PI-related gene families during c...The phosphatidylinositol (PI) metabolic pathway is considered critical in plant responses to many environmental factors,and previous studies have indicated the involvement of multiple PI-related gene families during cellular responses.Through a detailed analysis of the Arabidopsis thaliana genome,82 polypeptides were identified as being involved in PI signaling. These could be grouped into different families including PI synthases (PIS),PI-phosphate kinases (PIPK),phospholipases (PL),inositol polyphosphate phosphatases (IPPase),inositol polyphosphate kinases (IPK),PI transfer proteins and putative inositol polyphosphate receptors. The presence of more than 10 isoforms of PIPK,PLC,PLD and IPPase suggested that these genes might be differentially expressed during plant cellular responses or growth and development. Accordingly,DNA chip technology was employed to study the expression patterns of various isoforms.In total,79 mRNA clones were amplified and used for DNA chip generation. Expression profile analysis was performed using samples that represented multiple tissues or cellular responses. Tested samples included normal leaf,stem and flower tissues,and leaves from plants treated with various hormones (auxin,cytokinin,gibberellin,abscisic acid and brassinosteroid) or environmental factors (temperature,calcium,sodium,drought,salicylic acid and jasmonic acid).Results showed that many PI pathway-related genes were differentially expressed under these experimental conditions.In particular,the different isoforms of each family were specifically expressed in many cases,suggesting their involvement in tissue specificity and cellular responses to environmental conditions. This work provides a starting point for functional studies of the relevant PI-related proteins and may help shed light onto the role of PI pathways in development and cellular responses.展开更多
DNA chip technology employs light- directed in situ oligonucleotide synthesis and/or DNA microarray printing device to produce arrays of large number of probes in the tiny surface of silicon substrates, which makes it...DNA chip technology employs light- directed in situ oligonucleotide synthesis and/or DNA microarray printing device to produce arrays of large number of probes in the tiny surface of silicon substrates, which makes it possible that the gene detection be conducted efficiently with high speed and sensitivity. The DNA chip may take important part in genome research, gene diagnoses and so on.展开更多
基金Supported by National Natural Science Foundation of China(30700597)~~
文摘Accompanying with the increasingly saturated genome figures,DNA chip has been widely applied.Thanks to its advantages of integration,miniaturization and automation,DNA chip becomes a powerful research tool in various research fields including biology,medicine and chemistry.This article overviews the application of DNA chip technology in animal medicine from gene expression spectrum research,pathogenic microbial detection,bacterial typing,genetic mutations and polymorphism detection,pathogenic microbial genomics research,as well as its principle and classification.
基金supported by grants from the National Natural Science Foundation of China(No.30100101)the State Key Project of Basic Research(No.G19990-11604)
文摘The phosphatidylinositol (PI) metabolic pathway is considered critical in plant responses to many environmental factors,and previous studies have indicated the involvement of multiple PI-related gene families during cellular responses.Through a detailed analysis of the Arabidopsis thaliana genome,82 polypeptides were identified as being involved in PI signaling. These could be grouped into different families including PI synthases (PIS),PI-phosphate kinases (PIPK),phospholipases (PL),inositol polyphosphate phosphatases (IPPase),inositol polyphosphate kinases (IPK),PI transfer proteins and putative inositol polyphosphate receptors. The presence of more than 10 isoforms of PIPK,PLC,PLD and IPPase suggested that these genes might be differentially expressed during plant cellular responses or growth and development. Accordingly,DNA chip technology was employed to study the expression patterns of various isoforms.In total,79 mRNA clones were amplified and used for DNA chip generation. Expression profile analysis was performed using samples that represented multiple tissues or cellular responses. Tested samples included normal leaf,stem and flower tissues,and leaves from plants treated with various hormones (auxin,cytokinin,gibberellin,abscisic acid and brassinosteroid) or environmental factors (temperature,calcium,sodium,drought,salicylic acid and jasmonic acid).Results showed that many PI pathway-related genes were differentially expressed under these experimental conditions.In particular,the different isoforms of each family were specifically expressed in many cases,suggesting their involvement in tissue specificity and cellular responses to environmental conditions. This work provides a starting point for functional studies of the relevant PI-related proteins and may help shed light onto the role of PI pathways in development and cellular responses.
基金This work was supported by the China National Science Foundation (No.39880032)
文摘DNA chip technology employs light- directed in situ oligonucleotide synthesis and/or DNA microarray printing device to produce arrays of large number of probes in the tiny surface of silicon substrates, which makes it possible that the gene detection be conducted efficiently with high speed and sensitivity. The DNA chip may take important part in genome research, gene diagnoses and so on.
基金Natural Science Foundation of Hunan Province(2022JJ40287)Excellent Youth Program of Hunan Education Department (21B0081)+1 种基金Hunan Provincial Administration of Traditional Chinese Medicine (D2022027)Natural Science Foundation of Changsha (kq2202255)。
