This paper describes in a general way the objectives, contents and methods of molecular breeding, and the application of molecular breeding in hybrid rice.
The new cytoplasmic male sterile （CMS） line Yewei A and its maintainer line Yewei B, with better agronomic characteristics, have been developed from a mutant of V20B （a rice maintainer line） through transformation...The new cytoplasmic male sterile （CMS） line Yewei A and its maintainer line Yewei B, with better agronomic characteristics, have been developed from a mutant of V20B （a rice maintainer line） through transformation of genomic DNA of wild rice （Oryza minuta J. S. Presl. ex C. B. Presl.）. Analysis of molecular markers, DNA sequences, and Southern blot revealed that high DNA polymorphism exists between the mutant and its receptor, indicating that the special DNA fragment from O. minuta may be integrated into the genome of Yewei B. Therefore, transformation of genomic DNA from distant relatives to the plant of a target receptor may open an avenue for creating a new rice germplasm.展开更多
文摘This paper describes in a general way the objectives, contents and methods of molecular breeding, and the application of molecular breeding in hybrid rice.
文摘The new cytoplasmic male sterile （CMS） line Yewei A and its maintainer line Yewei B, with better agronomic characteristics, have been developed from a mutant of V20B （a rice maintainer line） through transformation of genomic DNA of wild rice （Oryza minuta J. S. Presl. ex C. B. Presl.）. Analysis of molecular markers, DNA sequences, and Southern blot revealed that high DNA polymorphism exists between the mutant and its receptor, indicating that the special DNA fragment from O. minuta may be integrated into the genome of Yewei B. Therefore, transformation of genomic DNA from distant relatives to the plant of a target receptor may open an avenue for creating a new rice germplasm.
