Accurate typing of the different human papillomavirus types is csscntial in view of the differ-ent pathological potential of the common virus types of human papillomavirus （HPV） present in thecervix. We have develop...Accurate typing of the different human papillomavirus types is csscntial in view of the differ-ent pathological potential of the common virus types of human papillomavirus （HPV） present in thecervix. We have developed hybridization, washing and autoradiography conditions that minimize thecross-hybridization among different specific types of HPV so as to allow clear - cut type assignmentthrough practical dot blot hybridization technique using nylon membrane and <sup>35</sup>S - labeled HPV - 16DNA probe. Under these conditions seventeen of thirty （56.7%） of squamous cell carcinomas of thecervix uteri obtained from Tianjin women were detected in the presence of HPV - 16 DNA.展开更多
Genetic mutations are important molecular biomarkers for cancer diagnosis and surveillance. Therefore, the development of methods for mutation detection characterized with straightforward, highly specific and sensitiv...Genetic mutations are important molecular biomarkers for cancer diagnosis and surveillance. Therefore, the development of methods for mutation detection characterized with straightforward, highly specific and sensitive to low-level mutations within various sequence contexts is extremely needed. Although some of the currently available methods have shown very encouraging results, their discrimination efficiency is still very low. Herein, we demonstrate a fluorescent probe coupled with blocker and property of melting temperature discrimination, which is able to identify the presence of known or unknown single-base variations at abundances down to 0.1% within 20 min. The discrimination factors between the perfect-match target and single-base mismatched target are determined to be 10.15–38.48. The method is sequence independent, which assures a wide range of application. The new method would be an ideal choice for high-throughput in vitro diagnosis and precise clinical treatment.展开更多
Subject Code:B05 With the support by the National Natural Science Foundation of China and the US National Institutes of Health,a team led by Dr.Tan Weihong(谭蔚泓)at Hunan University and the University of Florida repo...Subject Code:B05 With the support by the National Natural Science Foundation of China and the US National Institutes of Health,a team led by Dr.Tan Weihong(谭蔚泓)at Hunan University and the University of Florida reported a new DNA probe for studying cell membrane interactions,which was recently published展开更多
Nanocarriers have been developed to deliver DNA probes into cells for imaging analysis and improving their nuclease resist-ance.However,we found obvious lowered sensitivity and even false-negative results in the tradi...Nanocarriers have been developed to deliver DNA probes into cells for imaging analysis and improving their nuclease resist-ance.However,we found obvious lowered sensitivity and even false-negative results in the traditional DNA detachment-dependent fluorescent lighting mechanism.Here,we developed a detachment-independent fluorescent lighting mechanism integrated with cationic dipeptide nanoparticles(CDNs).CDNs displayed little quenching effect on fluorophores labeled on probes and improved the nuclease resistance of probes.In contrast to the traditional beacon,the fluorescence lighting was significantly accelerated without the need of desorption of the recognition products from the nanosurface,avoiding false-negative results from non-specific conformational adsorption.This work will open up new thoughts to improve the sensing performance of DNA probes in cell imaging,such as speed and sensitivity.展开更多
文摘Accurate typing of the different human papillomavirus types is csscntial in view of the differ-ent pathological potential of the common virus types of human papillomavirus （HPV） present in thecervix. We have developed hybridization, washing and autoradiography conditions that minimize thecross-hybridization among different specific types of HPV so as to allow clear - cut type assignmentthrough practical dot blot hybridization technique using nylon membrane and <sup>35</sup>S - labeled HPV - 16DNA probe. Under these conditions seventeen of thirty （56.7%） of squamous cell carcinomas of thecervix uteri obtained from Tianjin women were detected in the presence of HPV - 16 DNA.
文摘Genetic mutations are important molecular biomarkers for cancer diagnosis and surveillance. Therefore, the development of methods for mutation detection characterized with straightforward, highly specific and sensitive to low-level mutations within various sequence contexts is extremely needed. Although some of the currently available methods have shown very encouraging results, their discrimination efficiency is still very low. Herein, we demonstrate a fluorescent probe coupled with blocker and property of melting temperature discrimination, which is able to identify the presence of known or unknown single-base variations at abundances down to 0.1% within 20 min. The discrimination factors between the perfect-match target and single-base mismatched target are determined to be 10.15–38.48. The method is sequence independent, which assures a wide range of application. The new method would be an ideal choice for high-throughput in vitro diagnosis and precise clinical treatment.
文摘Subject Code:B05 With the support by the National Natural Science Foundation of China and the US National Institutes of Health,a team led by Dr.Tan Weihong(谭蔚泓)at Hunan University and the University of Florida reported a new DNA probe for studying cell membrane interactions,which was recently published
基金supported in part by the financial support through the National Natural Science Foundation of China(22074008,91853104,21735001,21705010)the Natural Science Foundation of Hunan Province(2019JJ30025).
文摘Nanocarriers have been developed to deliver DNA probes into cells for imaging analysis and improving their nuclease resist-ance.However,we found obvious lowered sensitivity and even false-negative results in the traditional DNA detachment-dependent fluorescent lighting mechanism.Here,we developed a detachment-independent fluorescent lighting mechanism integrated with cationic dipeptide nanoparticles(CDNs).CDNs displayed little quenching effect on fluorophores labeled on probes and improved the nuclease resistance of probes.In contrast to the traditional beacon,the fluorescence lighting was significantly accelerated without the need of desorption of the recognition products from the nanosurface,avoiding false-negative results from non-specific conformational adsorption.This work will open up new thoughts to improve the sensing performance of DNA probes in cell imaging,such as speed and sensitivity.
