A novel DNA photocleaver containing bromoacetophenone-polyamide was synthesized by DCC coupling reaction in the presence of DMAP, and its DNA cleaving activity was investigated by using pBluescript SK DNA by method of...A novel DNA photocleaver containing bromoacetophenone-polyamide was synthesized by DCC coupling reaction in the presence of DMAP, and its DNA cleaving activity was investigated by using pBluescript SK DNA by method of gel electrophoresis. The result shows that the photocleaver containing bromoacetophenone-polyamide has a remarkable cleaving activity for pBluescript SK DNA.展开更多
The [2] pseudorotaxane of cucurbit[6]uril(CB[6]) with guest molecule 1,6-bis(imidazol-1-yl)hexane dihydrochloride(BIMH-Cl) made pBR322 DNA hydrolyzed efficiently in physiological environment. The cleavage mechanism wa...The [2] pseudorotaxane of cucurbit[6]uril(CB[6]) with guest molecule 1,6-bis(imidazol-1-yl)hexane dihydrochloride(BIMH-Cl) made pBR322 DNA hydrolyzed efficiently in physiological environment. The cleavage mechanism was proposed that was a cooperation process of the CB[6] molecule and the guest BIMH molecule. In this mechanism, the protonated imidazole might bind DNA via the electrostatic interactions and the CB[6] glycoluril carbonyl oxygen atoms activated a water molecule to attack the phosphorus atom.展开更多
文摘A novel DNA photocleaver containing bromoacetophenone-polyamide was synthesized by DCC coupling reaction in the presence of DMAP, and its DNA cleaving activity was investigated by using pBluescript SK DNA by method of gel electrophoresis. The result shows that the photocleaver containing bromoacetophenone-polyamide has a remarkable cleaving activity for pBluescript SK DNA.
文摘The [2] pseudorotaxane of cucurbit[6]uril(CB[6]) with guest molecule 1,6-bis(imidazol-1-yl)hexane dihydrochloride(BIMH-Cl) made pBR322 DNA hydrolyzed efficiently in physiological environment. The cleavage mechanism was proposed that was a cooperation process of the CB[6] molecule and the guest BIMH molecule. In this mechanism, the protonated imidazole might bind DNA via the electrostatic interactions and the CB[6] glycoluril carbonyl oxygen atoms activated a water molecule to attack the phosphorus atom.
