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狂犬病毒GSQY-Dog-China-2013株全基因组序列测定和遗传进化分析
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作者 邢广旭 姜中毅 +2 位作者 焦文强 王方雨 张改平 《中国兽医杂志》 CAS 北大核心 2023年第1期8-13,共6页
为了全面分析我国狂犬病病毒遗传进化情况,本试验使用反转录PCR(RT-PCR)方法将狂犬病病毒GSQY-Dog-China-2013分为10个片段进行扩增,回收目的片段并与pMD20-T simple载体连接,转化JM109感受态细胞,挑取阳性克隆进行测序;使用DNASTAR软... 为了全面分析我国狂犬病病毒遗传进化情况,本试验使用反转录PCR(RT-PCR)方法将狂犬病病毒GSQY-Dog-China-2013分为10个片段进行扩增,回收目的片段并与pMD20-T simple载体连接,转化JM109感受态细胞,挑取阳性克隆进行测序;使用DNASTAR软件中的Edit Seq通过拼接相邻片段之间的重叠序列获得GSQY-Dog-China-2013全基因组序列,构建系统进化树进行遗传进化分析。结果显示,GSQY-Dog-China-2013全长11924核苷酸(nt),包含5个开放阅读框,分别编码核蛋白(N)、磷蛋白(P)、基质蛋白(M)、糖蛋白(G)和转录大蛋白(L);基于全基因组序列的进化树分析显示,GSQY-Dog-China-2013株和国内分离株形成一个分支,在国内分离株中与SXYL15株和SXBJ15株形成一个分支,亲缘关系最接近,提示GSQY-Dog-China-2013可能源自我国陕西省。本试验系甘肃省首次报道狂犬病病毒全基因组序列,进一步丰富了我国狂犬病病毒遗传进化数据,为狂犬病全面防治以及疫苗研发提供了数据支持。 展开更多
关键词 狂犬病病毒 GSQY-dog-China-2013 全基因组序列测定 遗传进化分析
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Amino acid nutrition and metabolism in domestic cats and dogs
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作者 Peng Li Guoyao Wu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第5期1777-1797,共21页
Domestic cats and dogs are carnivores that have evolved differentially in the nutrition and metabolism of amino acids.This article highlights both proteinogenic and nonproteinogenic amino acids.Dogs inadequately synth... Domestic cats and dogs are carnivores that have evolved differentially in the nutrition and metabolism of amino acids.This article highlights both proteinogenic and nonproteinogenic amino acids.Dogs inadequately synthesize citrulline(the precursor of arginine)from glutamine,glutamate,and proline in the small intestine.Although most breeds of dogs have potential for adequately converting cysteine into taurine in the liver,a small proportion(1.3%-2.5%)of the Newfoundland dogs fed commercially available balanced diets exhibit a deficiency of taurine possibly due to gene mutations.Certain breeds of dogs(e.g.,golden retrievers)are more prone to taurine deficiency possibly due to lower hepatic activities of cysteine dioxygenase and cysteine sulfinate decarboxylase.De novo synthesis of arginine and taurine is very limited in cats.Thus,concentrations of both taurine and arginine in feline milk are the greatest among domestic mammals.Compared with dogs,cats have greater endogenous nitrogen losses and higher dietary requirements for many amino acids(e.g.,arginine,taurine,cysteine,and tyrosine),and are less sensitive to amino acid imbalances and antagonisms.Throughout adulthood,cats and dogs may lose 34%and 21%of their lean body mass,respectively.Adequate intakes of high-quality protein(i.e.,32%and 40%animal protein in diets of aging dogs and cats,respectively;dry matter basis)are recommended to alleviate aging-associated reductions in the mass and function of skeletal muscles and bones.Pet-food grade animal-sourced foodstuffs are excellent sources of both proteinogenic amino acids and taurine for cats and dogs,and can help to optimize their growth,development,and health. 展开更多
关键词 Animal-sourced foodstuffs CATS dogS Health METABOLISM NUTRITION
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Impact of Fluorescent Light Energy on the Quality of Life of Dogs with Dermatologic Disease and Their Owners
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作者 Marion Mosca Amaury Briand +2 位作者 Isaac Carrasco Luca Luciani Oscar Fantini 《Open Journal of Veterinary Medicine》 2023年第7期122-135,共14页
Background: Quality of life (QoL) is a term used to evaluate general well-being, and it is defined as ‘the degree to which an individual enjoys his or her life. Within the realm of medicine, the evaluation of QoL fre... Background: Quality of life (QoL) is a term used to evaluate general well-being, and it is defined as ‘the degree to which an individual enjoys his or her life. Within the realm of medicine, the evaluation of QoL frequently involves examining how disease exerts a detrimental impact, diminishing the enjoyment and fulfilment experienced by the individual. Dermatological diseases have been found to exert a substantial negative influence on the QoL of dogs and their owners due to nuisance and stress related to the disease but also due to the caregiver burden. In the management of bacterial skin infections, topical therapy is commonly administered alongside systemic antibiotics. Nonetheless, the protracted duration of treatment and difficulties in ensuring owner compliance can introduce a significant caregiver burden, potentially exacerbating the challenges associated with these conditions. Purpose: This study aimed to evaluate the impact on the quality of life (QoL) of dogs with dermatologic diseases after fluorescent light energy (FLE) treatment. Methods: The study was an open, prospective, multicentric clinical trial that included dogs with various dermatological conditions. The dogs received FLE treatment once weekly until the clinical resolution was achieved. Owners completed a validated questionnaire to assess the QoL of their dogs before and after therapy. Results: Thirty-five dogs with deep pyoderma, interdigital furunculosis, pyotraumatic dermatitis, wounds and perianal fistulas were included. All dogs received two sessions of fluorescent light energy once a week. Median treatment duration was 9 weeks for perianal fistula, 7 weeks for interdigital furunculosis, 5 weeks for deep pyoderma, 3 weeks for wounds and 1.5 weeks for pyotraumatic dermatitis. Complete remission was noted in 86% of dogs, and 14% showed an improvement but partial remission. The majority of owners reported a positive impact on their dogs’ QoL after therapy, and 74% of the dogs showed at least a 50% reduction in QoL scores. Conclusion: Fluorescent light energy has been shown to exert beneficial effects on the healing of dermatological diseases and the quality of life (QoL) in dogs and their owners, whether used as a standalone treatment or in combination with standard care therapies. Additionally, it was well-tolerated by the dogs. This study emphasizes the significance of considering both the owner’s and dog’s QoL when evaluating the therapeutic efficacy of dermatological treatments. 展开更多
关键词 Fluorescent Light Energy Quality of Life dog DERMATOLOGY
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Therapeutic Effects of an Alpha-Casozepine and White Fish Muscle Hydrolyzed Complementary Feed on Canine Separation-Related Disorders and Quality of Life of Dogs and Their Pet Owners
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作者 Gaëlle Porcheron Marie Bodet +4 位作者 Katell Poiron Julie Brault Nicolas Massal Olivier Roy Oscar Fantini 《Open Journal of Veterinary Medicine》 2023年第6期68-81,共14页
Background: Separation-related disorders are prevalent in dogs and can result in problem behaviours such as inappropriate elimination, destructive behaviour, and distress vocalizations which occur when an owner is abs... Background: Separation-related disorders are prevalent in dogs and can result in problem behaviours such as inappropriate elimination, destructive behaviour, and distress vocalizations which occur when an owner is absent or perceived as absent. Numerous natural products are marketed for managing anxiety in dogs;however, few, if any, of these products have documented evidence of efficacy in veterinary behaviour. Purpose: This open, multicentric clinical trial aimed to assess the efficacy of a new supplement containing alpha-casozepine and fish muscle hydrolysate on the separation-related problem in dogs and the impact on the quality of life of dogs and their pet owners. Material and Methods: Fifty-one dogs with clinical signs compatible with separation-related problems were included. The dogs were treated with a complementary feed (Zylkene Plus, Vetoquinol, France) for 30 days. Separation-related behaviour assessment (SRP), emotional disorders assessment (EDED) and the quality of life (QoL) of the pets and their pet owner were assessed at baseline and the end of the study by the veterinarians. In addition, owners were asked to rate their dog’s SRP weekly after enrolment using the same questionnaire that the veterinarian used at the beginning of the study. Results: There was a statistically significant improvement in the SRP, EDED, and QoL scores (P Conclusion: The results of this study support the hypothesis that the combination of alpha-casozepine and fish muscle hydrolysate is a promising and effective option to reduce the frequency and severity of separation-related behaviour in dogs and improve the quality of dogs and their owners. 展开更多
关键词 Alpha-Casozepine Fish Muscle Hydrolysate Canine Separation-Related Disorders dogS
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Stray Dogs Problem and Right Approach to Prevent Attack
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作者 Halil Ecel Serap Göncü 《Journal of Environmental Science and Engineering(A)》 2023年第3期106-115,共10页
In this study,it is aimed to examine the knowledge level of reading stray dogs’behavior and the correct approach.Understanding the aggression of dogs and understanding their behavior is very important for stray dogs ... In this study,it is aimed to examine the knowledge level of reading stray dogs’behavior and the correct approach.Understanding the aggression of dogs and understanding their behavior is very important for stray dogs and dog owners in social life.Questionnaires prepared online were shared with 279 people of different ages and genders working in different fields in 50 provinces located in different regions,and the feedback obtained by answering voluntarily was taken into consideration.As a result of the study,when a dangerous-looking dog is encountered,it is necessary to stay as calm as possible and start to walk away slowly,without losing sight of the dog,with short steps,and without running under any circumstances.But without turning your back,because turning your back on the dog is a sign of weakness and an invitation to attack.While 36%left unanswered the question of what should be done at the time of the dog attack,20.1%replied by saying that they would stand calmly and shout and scare,and 20.9%would shout and stop.The results of the survey show that there are inaccuracies and deficiencies in both the body language of the dogs and the way they approach the dog.The participants answered the frequency of encountering stray animals by saying 75.9%daily.A safe approach to dogs or knowing the suitable approaches to calm a dangerous dog is very important in eliminating the dangers.These results show that there is a need for correct,informative activities on dog behavior and the right approach. 展开更多
关键词 Stray dogs PROBLEM APPROACH KNOWLEDGE
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An Ecofeminism Interpretation of Ian McEwan’s Black Dogs
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作者 HE Yuan 《Journal of Literature and Art Studies》 2023年第10期757-762,共6页
Ian McEwan’s Black Dogs reflects the anxiety and panic after the Second World War vividly with charming words.Based on Ian McEwan’s Black Dogs,many scholars have explored and acquired great achievements in trauma wr... Ian McEwan’s Black Dogs reflects the anxiety and panic after the Second World War vividly with charming words.Based on Ian McEwan’s Black Dogs,many scholars have explored and acquired great achievements in trauma writing and historical writing.Through analyzing the similarities of the heroine’s long-term neglected inner needs and the cruelly killed creatures in nature,this paper finds there is an intimate connection between female and nature in Black Dogs.However,instead of being in a constant state of repression,female and nature will fight back.To sum up,this paper attempts to make an ecofeminism interpretation of Ian McEwan’s Black Dogs.A conclusion may be reached that June’s spiritual awakening resonates with enhancement of nature’s power. 展开更多
关键词 Ian McEwan Black dogs ECOFEMINISM
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Dog Mania
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作者 《疯狂英语(初中天地)》 2023年第5期62-64,共3页
What is the number one favourite pet in the UK?If you think the answer is“dogs”-you are wrong.There are 8.6 million cats and 8.3 million dogs in Britain according to recent figures.But experts predict that there wil... What is the number one favourite pet in the UK?If you think the answer is“dogs”-you are wrong.There are 8.6 million cats and 8.3 million dogs in Britain according to recent figures.But experts predict that there will be more dogs than cats next year as dogs become more and more popular. 展开更多
关键词 BRITAIN dogS milli
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The Impact of a Pet, in This Case a Dog, on Physical Activity, Independence, Social Contacts, Health and Quality of Life of Elderly People
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作者 Anne Poestges Ursula Gresser Barbara Maria Richartz 《Advances in Aging Research》 2016年第4期83-95,共13页
In a rural area near Munich, 52 persons aged 65 years and older were interviewed in person about health and social life circumstances. Findings: Dog owners take significantly longer walks than people who do not own do... In a rural area near Munich, 52 persons aged 65 years and older were interviewed in person about health and social life circumstances. Findings: Dog owners take significantly longer walks than people who do not own dogs, averaging more than 120 minutes daily (p < 0.0001). On average, people who do not own a dog walked 38 minutes, cat owners 17 minutes. 94% of the dog owners were active daily compared to 14% of those who do not own a dog. Dog owners go to the doctor on average 5.3 times per year, people who do not own a dog 7.8 times. There was no difference between the two groups in regard to the use of medication. 80% would not move to a residential home for the elderly because of the dog, and 87% would want to take the dog along. 50% of the dog owners and 41% of those who do not own a dog had on average more than 3 contacts with other people, 6% of the dog owners and 23% of those who do not own a dog had less than one contact per day. 19% of the dog owners and 36% of those who do not own a dog would describe themselves as reserved in dealings with people. 71% stated that the dog had been very useful to help them overcome the loss of a person close to them. The W-5 [Wellbeing Five score] for dog owners was an average value of 75%, for those who did not own a dog 71%. Depression was uniformly distributed in both groups. The BMIs in both groups were similar. There was no correlation to the length of the daily walk. 展开更多
关键词 Elder People and dog Physical Activity and dog dog Walking dog and Life Quality Elder People Physical Activity
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CD117、PDGFRA蛋白单独及联合DOG1检测在胃肠道间质瘤中的诊断价值 被引量:26
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作者 许春伟 韩鸿雁 +9 位作者 王晶晶 张博 邵云 张立英 王怀涛 王海艳 吴永芳 李晓兵 李瑞明 田玉旺 《临床与病理杂志》 CAS 2015年第2期252-257,共6页
目的:探讨组织中CD117、PDGFRA两种蛋白表达在胃肠道间质瘤(gastrointestinal stromal tumors,GIST)中的诊断价值及联合DOG1蛋白检测的意义。方法:回顾性对99例GIST和25例非GIST肿瘤标本进行CD117、PDGFRA和DOG1蛋白表达进行检测并进行... 目的:探讨组织中CD117、PDGFRA两种蛋白表达在胃肠道间质瘤(gastrointestinal stromal tumors,GIST)中的诊断价值及联合DOG1蛋白检测的意义。方法:回顾性对99例GIST和25例非GIST肿瘤标本进行CD117、PDGFRA和DOG1蛋白表达进行检测并进行相关性分析。结果:GIST组CD117、PDGFRA和DOG1蛋白表达率分别为93.94%(93/99)、53.54%(53/99)和90.91%(90/99),非GIST组分别为4.00%(1/25)、4.00%(1/25)和12.00%(3/25),组间比较均有统计学意义(P<0.05);GIST组性别、年龄、肿瘤直径、肿瘤部位、组织学类型和危险度分级等临床病理参数与CD117、PDGFRA和DOG1蛋白表达无统计学意义(P>0.05);CD117、PDGFRA、DOG1、CD117和DOG1联合、PDGFRA和DOG1联合及三者联合判断GIST的敏感性分别为0.989、0.981、0.968、0.960、0.933和0.961,特异性分别为0.800、0.343、0.710、0.840、0.947和0.955,ROC曲线下面积(AUC)分别为0.945、0.748、0.895、0.895、0.840和0.975。结论:GIST中CD117、PDGFRA及DOG1蛋白表达在胃肠道间质瘤中的优势人群有待进一步研究;CD117、PDGFRA蛋白单独及联合DOG1检测可提高对GIST诊断的准确度。 展开更多
关键词 胃肠道间质瘤 CD117 PDGFRA dog1 诊断
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胃肠道间质瘤组织中DOG1与CD117、CD34的表达及意义 被引量:12
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作者 郑铭 江淑萍 +1 位作者 何毅辉 余惠珍 《中国老年学杂志》 CAS CSCD 北大核心 2012年第6期1165-1167,共3页
目的通过检测胃肠道间质瘤(GIST)中DOG1与CD117、CD34的表达,探讨DOG1与CD117、CD34的联合表达与GIST相关性及临床意义。方法应用免疫组化检测80例GIST中DOG1与CD117、CD34的表达,并与平滑肌肉瘤及神经鞘瘤进行对照分析。结果在GIST患者... 目的通过检测胃肠道间质瘤(GIST)中DOG1与CD117、CD34的表达,探讨DOG1与CD117、CD34的联合表达与GIST相关性及临床意义。方法应用免疫组化检测80例GIST中DOG1与CD117、CD34的表达,并与平滑肌肉瘤及神经鞘瘤进行对照分析。结果在GIST患者中,DOG1与CD117表达阳性率相比无统计学差异(P>0.05),DOG1与CD117表达阳性率明显高于CD34(P<0.01)的表达;与DOG1+/CD117+共同表达阳性率相比,DOG1+/CD34+(P<0.01)和CD117+/CD34+(P<0.05)的共同表达阳性率明显降低;在7例CD117阴性表达的GIST患者中,其中6例(85.7%)的DOG1呈阳性表达。不同性别、年龄组、部位及危险度之间DOG1表达阳性率的差异无统计学意义。结论 DOG1作为GIST一种新的标志物,具有与CD117同样的高敏感性;与CD117联合用于GIST的诊断具有较好的特异性和互补性,尤其对CD117阴性的GIST具有较大优势,但不能作为划分GIST危险度的指标。 展开更多
关键词 胃肠道间质瘤 dog1 CD117 病理诊断
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特异性生物标记物DOG1在胃肠间质瘤中的表达 被引量:5
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作者 杨军 刘妮 +3 位作者 康安静 靳耀锋 苏宝山 陈晓黎 《诊断病理学杂志》 CSCD 北大核心 2013年第5期282-285,共4页
目的探讨DOG1在胃肠间质瘤(GISTs)中的表达及其临床意义,并与CD117、CD34、SMA和S-100进行比较。方法收集68例GISTs病例,采用免疫组化SP法检测DOG1、CD117、CD34、SMA和S-100的表达,以瘤旁胃肠肌层组织和子宫平滑肌瘤、神经纤维瘤、纤... 目的探讨DOG1在胃肠间质瘤(GISTs)中的表达及其临床意义,并与CD117、CD34、SMA和S-100进行比较。方法收集68例GISTs病例,采用免疫组化SP法检测DOG1、CD117、CD34、SMA和S-100的表达,以瘤旁胃肠肌层组织和子宫平滑肌瘤、神经纤维瘤、纤维瘤作为对照。结果 68例GISTs中,DOG1、CD117、CD34阳性表达率分别为97.1%(66/68)、89.7%(61/68)、82.4%(56/68),DOG1和CD117的阳性率均显著高于CD34阳性率(P<0.05),且DOG1阳性率也显著高于CD117阳性率(P<0.05)。SMA和S-100在GISTs中均为(-)。结论 DOG1是胃肠间质瘤特异性生物标记,联合检测DOG1、CD117、CD34、SMA和S-100蛋白的表达对于确诊GISTs具有重要价值。 展开更多
关键词 胃肠间质瘤 C-KIT dog1 CD117
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DOG1、CD117和Ki67在胃肠道间质瘤中的表达及其与临床病理因素和危险度的相关性 被引量:15
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作者 曾祥 李岩 裴冬梅 《中国医科大学学报》 CAS CSCD 北大核心 2014年第5期449-453,共5页
目的探讨DOG1、CD117和Ki67在胃肠道间质瘤中表达及其与临床病理因素的相关性和危险度的关系。方法应用免疫组化方法检测DOG1、CD117和Ki67在66例GIST中表达情况,并对病例进行危险度分级,分析上述免疫组化指标与临床病理相关因素和危险... 目的探讨DOG1、CD117和Ki67在胃肠道间质瘤中表达及其与临床病理因素的相关性和危险度的关系。方法应用免疫组化方法检测DOG1、CD117和Ki67在66例GIST中表达情况,并对病例进行危险度分级,分析上述免疫组化指标与临床病理相关因素和危险度之间的关系。结果在66例GIST中,DOG1表达阳性率为93.94%,CD117表达阳性率为65.15%,其中CD117阴性的GIST中DOG1阳性表达率为82.61%。DOG1和CD117阳性表达率与性别、年龄、部位、肿瘤大小、核分裂数、危险度之间差异均无统计学意义(P>0.05)。但DOG1表达阳性率高于CD117,差异有统计学意义(P〈0.01)。Ki67增殖指数〈1%,1%~5%和>5%的例数分别为15例、20例、24例。Ki67增殖指数在GIST中阳性表达率为89.39%,Ki67的阳性表达与性别、年龄、部位之间差异无统计学意义(P>0.05),而与肿瘤大小、核分裂数及危险度之间差异有统计学意义(P〈0.01),并且Ki67增殖指数与肿瘤大小、核分裂数及危险度之间均呈正相关(r=0.488、r=0.425、r=0.550)。在CD117阳性的GIST中,Ki67阳性表达率高于DOG1阳性的GIST,两者之间差异无统计学意义(P>0.05)。结论在GIST中,DOG1比CD117有更高的敏感性,Ki67增殖指数的表达可与肿瘤大小、核分裂数一起作为判断危险度及预测预后的重要指标。 展开更多
关键词 胃肠道间质瘤 dog1 CD117 Ki67增殖指数 危险度
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新型胃肠道间质瘤标记物DOG1的应用与探讨 被引量:16
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作者 赵晶洁 张学东 +1 位作者 李建华 武海生 《广东医学》 CAS CSCD 北大核心 2010年第7期896-898,共3页
目的探讨新型胃肠道间质瘤(GIST)标记物DOG1的敏感性和特异性。方法收集50例胃肠道间质瘤的完整临床资料及病理资料。复读切片,行免疫组化CD117、PDGFRA、DOG1标记,进行比较。另收集20例非GIST而CD117阳性的病例行DOG1标记。结果在不同... 目的探讨新型胃肠道间质瘤(GIST)标记物DOG1的敏感性和特异性。方法收集50例胃肠道间质瘤的完整临床资料及病理资料。复读切片,行免疫组化CD117、PDGFRA、DOG1标记,进行比较。另收集20例非GIST而CD117阳性的病例行DOG1标记。结果在不同危险程度分级,CD117、PDGFRA阴性、弱阳性、阳性的病例,DOG1均呈较强的阳性表达。另20例非GIST而CD117阳性病例,DOG1则多为阴性或弱阳性。结论DOG1是敏感、特异的胃肠道间质瘤标记物。DOG1与CD117、PDGFRA的联合使用进一步完善了诊断依据,无论对明确诊断或术后个性化靶向治疗,都有着非常重要的意义。 展开更多
关键词 胃肠道间质瘤 dog1 病理诊断
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Dog Bone栅型MOS场效应晶体管的等效宽长比计算 被引量:2
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作者 孟丽娅 刘泽东 +2 位作者 胡大江 岳陈平 喻依虎 《重庆大学学报(自然科学版)》 EI CAS CSCD 北大核心 2013年第6期98-102,共5页
Dog Bone栅型MOS场效应晶体管是一种对称性的、防辐射总剂量效应的版图结构。为了计算其等效宽长比,把它划分为常规结构的主MOS晶体管和非常规结构的边缘MOS晶体管2种类型的并联。借助Silvaco TCAD工艺与器件仿真工具构建了它的模型,分... Dog Bone栅型MOS场效应晶体管是一种对称性的、防辐射总剂量效应的版图结构。为了计算其等效宽长比,把它划分为常规结构的主MOS晶体管和非常规结构的边缘MOS晶体管2种类型的并联。借助Silvaco TCAD工艺与器件仿真工具构建了它的模型,分析了主MOS晶体管的宽度、长度和边缘MOS晶体管的多晶硅与有源区的交叠宽度对边缘MOS等效宽长比的影响,得到拟合的Dog Bone栅型MOS晶体管等效宽长比的计算公式。采用CSMC 0.5μm DPTMCMOS混合信号工艺制作了样管,对实验测量值和公式计算值进行比较,Dog Bone栅型MOS场效应晶体管的等效宽长比的计算公式与实验能够较好地吻合。 展开更多
关键词 dog BONE MOS 防辐射加固 器件仿真
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胃肠道间质瘤CD117、CD34和DOG1免疫组化染色的最适修复方法 被引量:5
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作者 何姣 罗启翅 +5 位作者 刘汝艺 夏涌然 梁鹏 李林丰 刘智 夏勤 《临床与实验病理学杂志》 CAS CSCD 北大核心 2018年第8期925-927,共3页
免疫组织化学(immunohistochemistry,IHC)染色是一种通过利用已知抗原或抗体来特异性标记待测组织中蛋白的方法,是目前病理诊断中不可或缺的重要手段。胃肠道间质瘤(gastrointestinal stromal tumors,GIST)是一种常发生于胃肠道的间叶... 免疫组织化学(immunohistochemistry,IHC)染色是一种通过利用已知抗原或抗体来特异性标记待测组织中蛋白的方法,是目前病理诊断中不可或缺的重要手段。胃肠道间质瘤(gastrointestinal stromal tumors,GIST)是一种常发生于胃肠道的间叶源性肿瘤[1]。近年来发现,联合应用CD117、CD34和DOG1等一系列抗体的免疫组化检测,在GIST的诊断和用药中扮演着至关重要的角色[2-5]。由于甲醛固定、石蜡包埋后组织中抗原决定簇存在一定程度的交联封闭,因此组织中的抗原不能与目标抗体进行有效结合,影响免疫组化的染色结果,导致结果不稳定[6-7]。 展开更多
关键词 胃肠道间质瘤 抗原修复 CD117 CD34 dog1
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胃肠道间质瘤124例DOG1、WISP-1表达与临床意义 被引量:5
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作者 蹇顺海 黄一凡 +1 位作者 文彬 刘钧 《临床与实验病理学杂志》 CAS CSCD 北大核心 2011年第7期707-711,716,共6页
目的探讨DOG1和WISP-1在胃肠道间质瘤(gastrointestinal stromal tumors,GIST)中诊断及预后的意义。方法收集川北医学院附属医院病理科124例有完整临床病理资料的GIST,应用免疫组化EnVision两步法检测了DOG1和WISP-1蛋白在124例GIST中... 目的探讨DOG1和WISP-1在胃肠道间质瘤(gastrointestinal stromal tumors,GIST)中诊断及预后的意义。方法收集川北医学院附属医院病理科124例有完整临床病理资料的GIST,应用免疫组化EnVision两步法检测了DOG1和WISP-1蛋白在124例GIST中的表达情况,并与非GIST进行对照研究;应用χ2检验及Spearmen检验对结果进行统计学分析。结果 124例GIST中DOG1的阳性表达率为96%(119/124),CD117的阳性变表达率为91.0%(111/124),两者在GIST中的表达水平差异无统计学意义(P>0.05);GIST与非GIST中DOG1的表达水平差异有统计学意义(P<0.001);本组病例中WISP-1阳性率为80.6%(100/124),其中极低风险性、低风险性、中风险性和高风险性的阳性表达率分别为44.4%(4/9)、66.7%(26/39)、86.9%(17/20)和95.0%(38/40),其阳性表达率与美国国立卫生研究院(NIH)风险分级呈正相关关系(P<0.001);GIST与非GIST中WISP-1的表达水平差异无统计学意义(P>0.05)。结论 DOG1是GIST中一个敏感又特异的标记物,与CD117联用能提高GIST的诊断准确率,临床上可将其作为鉴别消化道间叶源性肿瘤的一线抗体;WISP-1的表达可能和GIST恶性进程有关,有可能作为评估GIST生物学行为的指标。 展开更多
关键词 胃肠道肿瘤 间质瘤 dog1 WISP-1 预后
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基于DOG模型的线条检测算法 被引量:8
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作者 罗晓晖 李见为 《计算机辅助设计与图形学学报》 EI CSCD 北大核心 2003年第4期425-431,共7页
在计算机视觉领域 ,线条检测是图像低层次处理中一个重要的问题 文中提出一种基于双高斯差 (DOG)模型的线条检测算法 分析比较了DOG检测器和高斯及高斯 拉普拉斯二阶微分检测器在线条检测方面的性能 ,解释了DOG函数和高斯及高斯 拉普... 在计算机视觉领域 ,线条检测是图像低层次处理中一个重要的问题 文中提出一种基于双高斯差 (DOG)模型的线条检测算法 分析比较了DOG检测器和高斯及高斯 拉普拉斯二阶微分检测器在线条检测方面的性能 ,解释了DOG函数和高斯及高斯 拉普拉斯二阶微分函数之间的关系 ,还给出了DOG检测器的线条检测范围并在二维空间中分析了其线条检测机理 实验中选用了指纹图像 。 展开更多
关键词 计算机视觉 图像处理 线条检测算法 dog模型
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DOG1蛋白在胃肠道间质瘤中的表达意义及其与CD117表达的关系 被引量:4
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作者 李宝江 姜忠敏 +4 位作者 张立东 郑末 张亮 孙雅静 王守峰 《广东医学》 CAS CSCD 北大核心 2011年第18期2434-2436,共3页
目的探讨DOG1在胃肠道间质瘤(GIST)和胃肠道外间质瘤(EGIST)的表达及临床意义,并与CD117表达比较,为进一步提高GIST的病理诊断及临床治疗提供有力的依据。方法收集胃肠道间叶源性肿瘤102例,其中47例GIST肿瘤组织及55例其他间叶源性肿瘤... 目的探讨DOG1在胃肠道间质瘤(GIST)和胃肠道外间质瘤(EGIST)的表达及临床意义,并与CD117表达比较,为进一步提高GIST的病理诊断及临床治疗提供有力的依据。方法收集胃肠道间叶源性肿瘤102例,其中47例GIST肿瘤组织及55例其他间叶源性肿瘤组织,采用免疫组织化学染色法检测DOG1在所有GIMT和CD117在GIST中的表达情况,并分析DOG1的表达与GIST临床病理参数、CD117表达及其他间叶源性肿瘤组织中表达的关系。结果 (1)GIST中DOG1和CD117蛋白表达率分别为95.73%(45/47),93.6%(44/47),与患者性别、年龄、肿瘤发生部位、细胞形态及Fletcher危险程度分级均无显著相关(P>0.05),2例CD117阴性GIST DOG1蛋白均强阳性表达,但两者表达率差异无统计学意义。(2)DOG1在其他间叶源性肿瘤表达率为1.8%(1/55),与其在GIST中表达比较差异有统计学意义。结论 DOG1是GIST敏感而特异性标记物,与CD117联合检测能够进一步提高GIST的诊断水平。 展开更多
关键词 胃肠道间质瘤 dog1 CD117
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DOG1在胃肠道间质瘤中的表达及其临床意义 被引量:5
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作者 艾力.赛丁 艾克拜尔.艾力 +3 位作者 张成 王志 阿丽叶古丽·艾皮热 克力木·阿不都热依木 《现代肿瘤医学》 CAS 2016年第21期3418-3421,共4页
目的:探讨DOG1在胃肠道间质瘤(GIST)中的表达及其临床意义。方法:免疫组化Envision法对73例GIST和26例非GIST间叶源性肿瘤检测DOG1的表达,分析DOG1与GIST的各项临床病理参数之间的关系。结果:在73例GIST中DOG1、CD117和CD34的阳性率分别... 目的:探讨DOG1在胃肠道间质瘤(GIST)中的表达及其临床意义。方法:免疫组化Envision法对73例GIST和26例非GIST间叶源性肿瘤检测DOG1的表达,分析DOG1与GIST的各项临床病理参数之间的关系。结果:在73例GIST中DOG1、CD117和CD34的阳性率分别为91.78%、94.52%和91.78%,而在26例非GIST中阳性率为11.54%、3.85%和23.08%,以上指标在两组间的表达有统计学差异(P=0.000);DOG1检测GIST灵敏度和特异度分别为91.80%、88.50%,而DOG1联合CD117检测GIST灵敏度和特异度分别为98.60%和84.60%,灵敏度最高;DOG1的表达与GIST的性别、年龄、肿瘤的位置、肿瘤的大小、核分裂象和恶性潜能分级等临床病理因素无相关性(P>0.05),同时DOG1的表达与GIST的预后也无相关性(P>0.05)。结论:在GIST的诊断中DOG1与CD117一样是敏感而特异的指标,尤其是对CD117阴性的GIST的诊断中,能够起很好的补充作用,但不能作为评价GIST预后的指标。 展开更多
关键词 胃肠道间质瘤 dog1 免疫组化
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CD117阴性的胃肠道间质瘤中DOG1的表达及意义 被引量:3
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作者 田青青 袁静萍 +2 位作者 王渝 况东 王国平 《临床与实验病理学杂志》 CAS CSCD 北大核心 2011年第7期763-765,共3页
目的探讨CD117阴性的胃肠道间质瘤(gastrointesti-nal stromal tumor,GIST)中DOG1的表达及意义。方法通过免疫组化法检测58例CD117阴性的GIST中DOG1的表达。结果 DOG1的阳性表达率为93.1%(54/58)。DOG1的表达与临床病理相关因素(性别、... 目的探讨CD117阴性的胃肠道间质瘤(gastrointesti-nal stromal tumor,GIST)中DOG1的表达及意义。方法通过免疫组化法检测58例CD117阴性的GIST中DOG1的表达。结果 DOG1的阳性表达率为93.1%(54/58)。DOG1的表达与临床病理相关因素(性别、年龄、部位、肿瘤大小)无相关性(P>0.05)。按危险程度分级分类:极低度8例,低度12例,中度21例,高度17例。DOG1的阳性率在不同危险程度间差异无显著性(P>0.05)。结论 DOG1是比CD117更敏感、更特异的诊断GIST的免疫学指标,但不能作为判断其危险程度的指标。 展开更多
关键词 胃肠道间质瘤 dog1 CD117
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