上世纪50年代,减肥手术开始用于治疗肥胖症,最初的临床观察发现,肥胖患者术后不仅体重显著下降,而且其并发的非胰岛素依赖型糖尿病得到意想不到的治愈或改善。但这一现象并未引起关注,至1998年,East Carolina大学Brody医学院的Pories等...上世纪50年代,减肥手术开始用于治疗肥胖症,最初的临床观察发现,肥胖患者术后不仅体重显著下降,而且其并发的非胰岛素依赖型糖尿病得到意想不到的治愈或改善。但这一现象并未引起关注,至1998年,East Carolina大学Brody医学院的Pories等在Annals of Surgery杂志报道了减肥手术后的随访研究结果,才引起了学术界的高度重视。展开更多
The objective of this study was to isolate and identify the intracellular bioactive peptides from mouse lymphocytes before and after lipopolysaccharide(LPS)stimulation,to explore novel peptides and to research the bio...The objective of this study was to isolate and identify the intracellular bioactive peptides from mouse lymphocytes before and after lipopolysaccharide(LPS)stimulation,to explore novel peptides and to research the bioactive function.Mouse spleen lymphocytes were isolated and cultured with LPS stimulation(experimental group)or not(control group)to collect intracellular peptides.Totally 385 peptides were analyzed by nanoliter liquid phase-Q Exactive quadrupole ultra-high resolution orbitrap mass spectrometer(Nano LC-Q Exactive Plus)and identifi ed by PEAKS X software.After compared with peptides reported,131 novel peptides were discovered,which then were predicted bioactivity by Peptide Ranker and 6 peptides with high bioactivity were predicted function by BIOPEP-UMW database.Prediction data showed that they may have dipeptidyl peptidase IV(DPP-IV)inhibitory activity.Finally,two peptides showed better potent inhibition were verifi ed with competitive and noncompetitive modes.展开更多
文摘上世纪50年代,减肥手术开始用于治疗肥胖症,最初的临床观察发现,肥胖患者术后不仅体重显著下降,而且其并发的非胰岛素依赖型糖尿病得到意想不到的治愈或改善。但这一现象并未引起关注,至1998年,East Carolina大学Brody医学院的Pories等在Annals of Surgery杂志报道了减肥手术后的随访研究结果,才引起了学术界的高度重视。
文摘The objective of this study was to isolate and identify the intracellular bioactive peptides from mouse lymphocytes before and after lipopolysaccharide(LPS)stimulation,to explore novel peptides and to research the bioactive function.Mouse spleen lymphocytes were isolated and cultured with LPS stimulation(experimental group)or not(control group)to collect intracellular peptides.Totally 385 peptides were analyzed by nanoliter liquid phase-Q Exactive quadrupole ultra-high resolution orbitrap mass spectrometer(Nano LC-Q Exactive Plus)and identifi ed by PEAKS X software.After compared with peptides reported,131 novel peptides were discovered,which then were predicted bioactivity by Peptide Ranker and 6 peptides with high bioactivity were predicted function by BIOPEP-UMW database.Prediction data showed that they may have dipeptidyl peptidase IV(DPP-IV)inhibitory activity.Finally,two peptides showed better potent inhibition were verifi ed with competitive and noncompetitive modes.
