OBJECTIVE To explore the mechanism of action of Danshen-Gegen on coronary heart disease.METHODS First using network pharmacology,according to oral bioavailability and drug-like properties,taking Danshen-Gegen as the r...OBJECTIVE To explore the mechanism of action of Danshen-Gegen on coronary heart disease.METHODS First using network pharmacology,according to oral bioavailability and drug-like properties,taking Danshen-Gegen as the research object,obtaining its active ingredients and targets in the pharmacological analysis platform of Chinese medicine system,using TTD,DrugBank and Disgenet database to obtain coronary heart disease targets,the active componentcoronary heart disease target network was constructed by CytosCape3.6.1 software,and the target protein interaction network was constructed by String database,Finally,GO and KEGG enrichment analysis was performed on the target in the DAVID database.RESULTS 61 active ingredients were screened from Danshen-Gegen,and 68 targets related to coronary heart disease were selected.GO analysis showed that Danshen-Gegen is involved in many biological processes such as transcription and inflammation of RNA polymeraseⅡpromoter.The target of treating coronary heart disease is mainly concentrated in extracellular space,plasma membrane and nucleus to treat coronary heart disease.The main effects of treatment of coronary heart disease are zinc ion binding,cytokine activity and sequence-specific DNA binding.The results of KEGG analysis showed that the regulation of Danshen-Gegen includes signaling pathways such as HIF-10,PI3K-Akt,TNF and Jak-STAT.CONCLUSION The combination of Danshen-Gegen has 61 active ingredients to play a role in the treatment of coronary heart disease through 68 targets and Jak-STAT,PI3K-Akt and other signaling path⁃ways.The results of this study provide a reference for further study of the pharmacological effects of Danshen-Gegen.展开更多
OBJECTIVE Danshen injection and Honghua injection,traditional Chinese medicine(TCM)injections,made from the extracts of Salvia miltiorrhiza Bge.and Carthamus tinctorius L.,have a potential to be developed into the use...OBJECTIVE Danshen injection and Honghua injection,traditional Chinese medicine(TCM)injections,made from the extracts of Salvia miltiorrhiza Bge.and Carthamus tinctorius L.,have a potential to be developed into the useful drugs for the.As the common TCM injections,Danshen injection is often combined with Honghua injection to treat cardiovascular disease.The purpose of this study was to investigate the pharmacokinetic parameters of Honghua injection combined with Danshen injection when they were coadministered intravenously in human and rats through the tail vein.METHODS Single and multiple doses of Danshen injection to study Danshen injection on Honghua injection pharmacokinetics parameters and single and multiple doses of Honghua injection to study Honghua injection on Danshen injection pharmacokinetics parameters.The plasma concentrations of hydroxysafflor A(HSYA)and tanshinol and salvianolic acid B were determined by the reliable high-performance liquid chromatography(HPLC)method.The concentrations of HSYA in urine of rats and human were also determined by HPLC method.DAS 2.1.1software was adopted for calculating the pharmacokinetic parameters.RESULTS The simultaneous intravenous Honghua injection and salvia miltiorrhiza injiection significantly altered the pharmacokinetic parameters of both injections when compared with the individual intravenous administration of each injection.The area under the concentration-timecurve(AUC)and maximum plasma concentration(Cmax)of HSYA and tanshinol and salvianolic acid B were significantly increased.The cumulative urine excretion of HSYA in human and rats during 24 h was decreased after two drugs were administered simultaneously by the intravenous.CONCLUSION Honghua injection and Danshen injection interact with each other following simultaneous intravenous and they have a synergistic action.This experiment has identified the pharmacokinetic parameters and provided a rationale for the clinical use of the drug combination.展开更多
[Objectives]This study was conducted to establish the quality standard of Danshen Jianxin Capsules.[Methods]Orthogonal design was adopted to optimize the water extraction process by taking the amount of water added,ex...[Objectives]This study was conducted to establish the quality standard of Danshen Jianxin Capsules.[Methods]Orthogonal design was adopted to optimize the water extraction process by taking the amount of water added,extraction time and extraction times as factors,and the content of tanshinone I as the index.Salviae Miltiorrhizae,Notoginseng Radix Et Rhizoma and Radix Astragali in the capsules were qualitatively identified by TLC,and the content of tanshinone I in the capsules was determined by HPLC.[Results]The best water extraction process included the steps of adding 12 times of water each time,extracting the materials twice,for 1 h each time.In TLC identification,the test samples showed spots of the same color at the positions corresponding to tanshinone II A,ginsenoside Rg1,notoginsenoside R1 and astragaloside IV reference samples.[Conclusions]Danshen Jianxin Capsules was prepared by the water extraction method,which is characterized by high efficiency and being suitable for mass production.The quality control method is reliable,fast and accurate,which can effectively control the quality of the product.展开更多
Objective:To explore the effective components of Huangqi(Astragalus membranaceus(Fisch.)Bge.)and Danshen(Salvia miltiorrhiza Bge.)and predict their potential functional targets,to explore the effect and mechanism of H...Objective:To explore the effective components of Huangqi(Astragalus membranaceus(Fisch.)Bge.)and Danshen(Salvia miltiorrhiza Bge.)and predict their potential functional targets,to explore the effect and mechanism of Huangqi and Danshen ultrafine granule gel on wound vascular healing in rats by network pharmacology and experimental verification.Methods:Using the TCMSP database to screen the active components of two herbs and their potential targets,to search the target related to vascular injury through the Gene Card database,and to select the gene target with correlation coefficient greater than 10%.Then map the disease related target to the potential target of the compound,to obtain the common target,and to import the information to the String online analysis platform and Cytoscape to make the PPI map,and to carry on the topological analysis,KEGG and GO enrichment analysis through Metascape platform.The expression analysis of the main target genes of network pharmacology was verified by pathological section staining and optical density method to determine the content of vascular endothelial growth factor(VEGF)in tissues,the number of tissue new capillaries,and the wound healing rate.Results:A total of 115 active components and 297 target genes were selected from Huangqi and Danshen,and 123 target genes were intersected with vascular injury,among which IL6,AKT1,INS,VEGFA,TNF,EGF,CASP3,MAPK1,TP53 were the active genes with the highest intersection value.The pathways involved are mainly related to AGE-RAGE signaling pathway in diabetic complications,MAPK signaling pathway,Pathways in cancer,human cytomegalovirus infection,Fluid shear stress and atherosclerosis,Endocrine resistance,foxo signaling pathway etc.We experimentally validated VEGFA based on the primary target of vascular regeneration,the experimental results showed that there was different expression of VEGFA proteins in wound vascular healing process,and the quercetin,daidzein,luteolin,apigenin,tanshinone i of 115 active components were selected as the main compounds acting on the VEGFA.Conclusion:This study systematically reveals the material basis and mechanism of Huangqi and Danshen regulating wound vascular healing through multi-component,multi-target and multi-pathway and provides theoretical and scientific basis for the clinical application of Huangqi and Danshen.展开更多
目的分析复方丹参滴丸联合单硝酸异山梨酯治疗冠心病心绞痛有效性及安全性。方法计算机检索中国知网、维普、万方、PubMed、Web of Science数据库,检索时限为自建库起至2023年2月28日,搜集相关临床随机对照试验(RCT),根据纳排标准选择R...目的分析复方丹参滴丸联合单硝酸异山梨酯治疗冠心病心绞痛有效性及安全性。方法计算机检索中国知网、维普、万方、PubMed、Web of Science数据库,检索时限为自建库起至2023年2月28日,搜集相关临床随机对照试验(RCT),根据纳排标准选择RCT,使用Review Manager 5.3软件进行meta分析。结果纳入14项RCT,meta分析结果显示,观察组的总有效率[OR=5.01,95%CI(3.39,7.42),P<0.00001]、心绞痛发作次数[MD=-1.75,95%CI(-2.43,-1.07),P<0.00001]、心绞痛发作持续时间[MD=-1.87,95%CI(-2.47,-1.26)、P<0.00001]、总胆固醇[MD=-0.96,95%CI(-1.19,-0.73),P<0.00001]、三酰甘油[MD=-0.52,95%CI(-0.65,-0.40),P<0.00001]、不良反应[OR=0.64,95%CI(0.43,0.97),P=0.03]均优于对照组。结论复方丹参滴丸联合单硝酸异山梨酯+常规西药治疗冠心病心绞痛临床疗效显著,安全性高,但需更多设计严谨的多样本、多中心、双盲RCT提供证据支持。展开更多
文摘OBJECTIVE To explore the mechanism of action of Danshen-Gegen on coronary heart disease.METHODS First using network pharmacology,according to oral bioavailability and drug-like properties,taking Danshen-Gegen as the research object,obtaining its active ingredients and targets in the pharmacological analysis platform of Chinese medicine system,using TTD,DrugBank and Disgenet database to obtain coronary heart disease targets,the active componentcoronary heart disease target network was constructed by CytosCape3.6.1 software,and the target protein interaction network was constructed by String database,Finally,GO and KEGG enrichment analysis was performed on the target in the DAVID database.RESULTS 61 active ingredients were screened from Danshen-Gegen,and 68 targets related to coronary heart disease were selected.GO analysis showed that Danshen-Gegen is involved in many biological processes such as transcription and inflammation of RNA polymeraseⅡpromoter.The target of treating coronary heart disease is mainly concentrated in extracellular space,plasma membrane and nucleus to treat coronary heart disease.The main effects of treatment of coronary heart disease are zinc ion binding,cytokine activity and sequence-specific DNA binding.The results of KEGG analysis showed that the regulation of Danshen-Gegen includes signaling pathways such as HIF-10,PI3K-Akt,TNF and Jak-STAT.CONCLUSION The combination of Danshen-Gegen has 61 active ingredients to play a role in the treatment of coronary heart disease through 68 targets and Jak-STAT,PI3K-Akt and other signaling path⁃ways.The results of this study provide a reference for further study of the pharmacological effects of Danshen-Gegen.
基金The project supported by 2016-2018 Anhui University Research Platform Innovation Team
文摘OBJECTIVE Danshen injection and Honghua injection,traditional Chinese medicine(TCM)injections,made from the extracts of Salvia miltiorrhiza Bge.and Carthamus tinctorius L.,have a potential to be developed into the useful drugs for the.As the common TCM injections,Danshen injection is often combined with Honghua injection to treat cardiovascular disease.The purpose of this study was to investigate the pharmacokinetic parameters of Honghua injection combined with Danshen injection when they were coadministered intravenously in human and rats through the tail vein.METHODS Single and multiple doses of Danshen injection to study Danshen injection on Honghua injection pharmacokinetics parameters and single and multiple doses of Honghua injection to study Honghua injection on Danshen injection pharmacokinetics parameters.The plasma concentrations of hydroxysafflor A(HSYA)and tanshinol and salvianolic acid B were determined by the reliable high-performance liquid chromatography(HPLC)method.The concentrations of HSYA in urine of rats and human were also determined by HPLC method.DAS 2.1.1software was adopted for calculating the pharmacokinetic parameters.RESULTS The simultaneous intravenous Honghua injection and salvia miltiorrhiza injiection significantly altered the pharmacokinetic parameters of both injections when compared with the individual intravenous administration of each injection.The area under the concentration-timecurve(AUC)and maximum plasma concentration(Cmax)of HSYA and tanshinol and salvianolic acid B were significantly increased.The cumulative urine excretion of HSYA in human and rats during 24 h was decreased after two drugs were administered simultaneously by the intravenous.CONCLUSION Honghua injection and Danshen injection interact with each other following simultaneous intravenous and they have a synergistic action.This experiment has identified the pharmacokinetic parameters and provided a rationale for the clinical use of the drug combination.
基金Supported by Self-financed Scientific Research Project of Administration of Traditional Chinese Medicine of Guangxi Zhuang Autonomous Region(GXZYZ20210290)The First Batch of High-level Talent Research Project of Affiliated Hospital of Youjiang Medical University for Nationalities in 2019(Y20196311)Basic Scientific Research Ability Improvement Project for Young and Middle-aged Teachers in Guangxi Universities in 2020(2020KY13034).
文摘[Objectives]This study was conducted to establish the quality standard of Danshen Jianxin Capsules.[Methods]Orthogonal design was adopted to optimize the water extraction process by taking the amount of water added,extraction time and extraction times as factors,and the content of tanshinone I as the index.Salviae Miltiorrhizae,Notoginseng Radix Et Rhizoma and Radix Astragali in the capsules were qualitatively identified by TLC,and the content of tanshinone I in the capsules was determined by HPLC.[Results]The best water extraction process included the steps of adding 12 times of water each time,extracting the materials twice,for 1 h each time.In TLC identification,the test samples showed spots of the same color at the positions corresponding to tanshinone II A,ginsenoside Rg1,notoginsenoside R1 and astragaloside IV reference samples.[Conclusions]Danshen Jianxin Capsules was prepared by the water extraction method,which is characterized by high efficiency and being suitable for mass production.The quality control method is reliable,fast and accurate,which can effectively control the quality of the product.
文摘Objective:To explore the effective components of Huangqi(Astragalus membranaceus(Fisch.)Bge.)and Danshen(Salvia miltiorrhiza Bge.)and predict their potential functional targets,to explore the effect and mechanism of Huangqi and Danshen ultrafine granule gel on wound vascular healing in rats by network pharmacology and experimental verification.Methods:Using the TCMSP database to screen the active components of two herbs and their potential targets,to search the target related to vascular injury through the Gene Card database,and to select the gene target with correlation coefficient greater than 10%.Then map the disease related target to the potential target of the compound,to obtain the common target,and to import the information to the String online analysis platform and Cytoscape to make the PPI map,and to carry on the topological analysis,KEGG and GO enrichment analysis through Metascape platform.The expression analysis of the main target genes of network pharmacology was verified by pathological section staining and optical density method to determine the content of vascular endothelial growth factor(VEGF)in tissues,the number of tissue new capillaries,and the wound healing rate.Results:A total of 115 active components and 297 target genes were selected from Huangqi and Danshen,and 123 target genes were intersected with vascular injury,among which IL6,AKT1,INS,VEGFA,TNF,EGF,CASP3,MAPK1,TP53 were the active genes with the highest intersection value.The pathways involved are mainly related to AGE-RAGE signaling pathway in diabetic complications,MAPK signaling pathway,Pathways in cancer,human cytomegalovirus infection,Fluid shear stress and atherosclerosis,Endocrine resistance,foxo signaling pathway etc.We experimentally validated VEGFA based on the primary target of vascular regeneration,the experimental results showed that there was different expression of VEGFA proteins in wound vascular healing process,and the quercetin,daidzein,luteolin,apigenin,tanshinone i of 115 active components were selected as the main compounds acting on the VEGFA.Conclusion:This study systematically reveals the material basis and mechanism of Huangqi and Danshen regulating wound vascular healing through multi-component,multi-target and multi-pathway and provides theoretical and scientific basis for the clinical application of Huangqi and Danshen.
文摘目的分析复方丹参滴丸联合单硝酸异山梨酯治疗冠心病心绞痛有效性及安全性。方法计算机检索中国知网、维普、万方、PubMed、Web of Science数据库,检索时限为自建库起至2023年2月28日,搜集相关临床随机对照试验(RCT),根据纳排标准选择RCT,使用Review Manager 5.3软件进行meta分析。结果纳入14项RCT,meta分析结果显示,观察组的总有效率[OR=5.01,95%CI(3.39,7.42),P<0.00001]、心绞痛发作次数[MD=-1.75,95%CI(-2.43,-1.07),P<0.00001]、心绞痛发作持续时间[MD=-1.87,95%CI(-2.47,-1.26)、P<0.00001]、总胆固醇[MD=-0.96,95%CI(-1.19,-0.73),P<0.00001]、三酰甘油[MD=-0.52,95%CI(-0.65,-0.40),P<0.00001]、不良反应[OR=0.64,95%CI(0.43,0.97),P=0.03]均优于对照组。结论复方丹参滴丸联合单硝酸异山梨酯+常规西药治疗冠心病心绞痛临床疗效显著,安全性高,但需更多设计严谨的多样本、多中心、双盲RCT提供证据支持。
