Objective:To analyze the relationship between serum ICAM-1,lactate dehydrogenase(LDH)and coronary heart disease(CHD)by clinical detection of serum ICAM-1 and LDH Objective to investigate the relationship between the s...Objective:To analyze the relationship between serum ICAM-1,lactate dehydrogenase(LDH)and coronary heart disease(CHD)by clinical detection of serum ICAM-1 and LDH Objective to investigate the relationship between the severity of different types of coronary artery stenosis(CHD)and its role in the progression of CHD.Methods:180 patients with chest pain and coronary angiography were selected,including 145 patients with CHD diagnosed by coronary angiography and 35 patients without abnormal coronary angiography(control group).The 145 patients with CHD were further divided into mild partial stenosis group(a-chd,stenosis development≤49%),moderate partial stenosis group(b-chd,stenosis development 50%~74%)and severe partial stenosis group(c-chd,stenosis development≥75%).The levels of ICAM-1 and LDH in serum of patients were detected by ELISA,and the differences of ICAM-1 and LDH activities in different degrees of coronary artery disease and control group were statistically analyzed.Results:in the control group,HDL was higher than that in the c-chd group(P<0.05),neutrophils in the control group was lower than that in the c-chd group(P<0.05);ICAM-1,LDH and Gensini scores in the a-chd group,b-chd group and c-chd group were higher than those in the control group(P<0.05-P<0.01),and gradually increased with the aggravation of different coronary artery stenosis processes(P<0.05-P<0.01).The levels of serum ICAM-1 and LDH increased significantly with the increase of the number of CHD lesions(P<0.01).The levels of ICAM-1 and LDH were positively correlated with Gensini score(LDH r=0.409,P<0.01 and ICAM-1 r=0.463,P<0.01).The levels of ICAM-1 and neutrophils were positively correlated(r=0.208,P<0.01).The levels of LDH and ICAM-1 were negatively correlated with HDL(LDH r=-0.184,P<0.05 and ICAM-1)There was a negative correlation between LDH and total protein(r=-0.218,P<0.01),and a positive correlation between ICAM-1 and LDH(r=0.441,P<0.01).Conclusion:the levels of serum ICAM-1 and LDH gradually increase with the aggravation of different coronary artery stenosis,and there is a certain linear relationship between them.展开更多
Objective:To analyze the correlation between serum Nrf2 and GPX4 activity levels with coronary heart disease(CHD)and the severity of coronary artery disease,and to explore the role of ferroptosis mediated by its signa...Objective:To analyze the correlation between serum Nrf2 and GPX4 activity levels with coronary heart disease(CHD)and the severity of coronary artery disease,and to explore the role of ferroptosis mediated by its signaling pathway in the progression of CHD.Methods:A total of 540 patients suspected of CHD were selected for coronary angiography,of which 360 patients were diagnosed with CHD.The activity levels of Nrf2 and GPX4 in the serum of CHD patients were detected by ELISA,and the differences in the activities of Nrf2 and GPX4 in the presence or absence of CHD were statistically analyzed.Western blot detection of Nrf2 protein expression of peripheral blood mononuclear cells(PBMCS)in the control(CON)and CHD groups(90 cases each).The expression and significance of its signaling pathway in CHD patients were analyzed.Results:The activity levels of Nrf2 and GPX4 in the CHD group were lower than those in the CON group(P<0.05),and the expression levels of Nrf2 in PBMCs of the two groups were detected by Western blot.The protein expression level of Nrf2 in the CHD group(0.25±0.05)was down‑regulated compared with CON group(0.87±0.16)(P<0.05),indicating that Nrf2 protein expression level was low in CHD patients.Pearson correlation analysis showed that serum Nrf2 and GPX4 levels were negatively correlated with Gensini score(Nrf2:r=‑0.347,P<0.001;GPX4:r=-0.423,P=0.001).Nrf2 and GPX4 were negatively correlated with TG(Nrf2:r=-0.284,P<0.001;GPX4:r=-0.275,P=0.001),Nrf2 and GPX4 levels were negatively correlated with LDL(Nrf2:r=-0.418,P<0.001)0.05;(GPX4:r=-0.426,P<0.05),Nrf2 and GPX4 levels were positively correlated with HDL(Nrf2:r=0.318,P<0.05;GPX4:r=0.428,P<0.05),and Nrf2 was positively correlated with GPX4(r=0.456,P<0.01).Conclusion:The ferroptosis pathway mediated by the Nrf2‑GPX4 signaling pathway is closely related to the degree of coronary artery disease and the pathogenesis of CHD,and its mechanism may be related to the down‑regulation of the Nrf2‑GPX4 signaling pathway.展开更多
Background Angina pectoris has been recognized as one of the principal symptoms of aortic valve stenosis （AS）,even in patients without significant coronary artery disease （CAD）.However,the incidence of angina pect...Background Angina pectoris has been recognized as one of the principal symptoms of aortic valve stenosis （AS）,even in patients without significant coronary artery disease （CAD）.However,the incidence of angina pectoris and related CAD in such patients is controversial.There is continuing debate as to whether coronary angiography is necessary before aortic valve replacement （AVR） in patients with severe AS.The purpose of this study was to evaluate the incidence and predictors of CAD in patients with severe AS in a Korean population.Methods Data from all consecutive patients with severe AS undergoing AVR at a major tertiary cardiac and vascular center in Korea were entered in a prospective registry beginning in 1995.Clinical and echocardiographic follow-up data were recorded into the database annually.Significant CAD was defined as one or more major coronary arteries having an estimated narrowing of ≥70％ and left main coronary arteries having an estimated narrowing of ≥50％ on coronary angiography.We excluded patients with multiple valve disease,significant aortic regurgitation,or prior CAD or valve surgery.Results Totally 574 patients with severe AS （mean age,（65.9±9.6） years） were enrolled in this study.Significant CAD was found in 61 patients （10.6％）.Factors associated with increased likelihood of CAD were age,hypertension,diabetes mellitus,chronic renal failure,carotid disease,and aorta calcification.In Logistic regression analysis,the independent predictor of the presence of CAD was age （P=0.011）.The incidence of CAD increased significantly at 69.2 years of age.Having two risk factors for cardiovascular disease was the most useful cutoff to predict whether a patient was going to have significant CAD.Conclusions There was a low incidence of significant CAD in a population of Korean patients with severe AS.Therefore,coronary angiography before AVR will be considered in patients with multiple risk factors for cardiovascular disease or in patients more than 69 years of age without risk factors for cardiovascular disease.展开更多
基金General fund of National Natural Science Foundation of China(No.81970313)Science and technology research project of Anhui Province(No.1804h080246)+1 种基金Excellent talent fund of Bengbu Medical College(No.2019byyfyq004)Innovation plan for Postgraduates of Bengbu Medical College(No.byycx20025)。
文摘Objective:To analyze the relationship between serum ICAM-1,lactate dehydrogenase(LDH)and coronary heart disease(CHD)by clinical detection of serum ICAM-1 and LDH Objective to investigate the relationship between the severity of different types of coronary artery stenosis(CHD)and its role in the progression of CHD.Methods:180 patients with chest pain and coronary angiography were selected,including 145 patients with CHD diagnosed by coronary angiography and 35 patients without abnormal coronary angiography(control group).The 145 patients with CHD were further divided into mild partial stenosis group(a-chd,stenosis development≤49%),moderate partial stenosis group(b-chd,stenosis development 50%~74%)and severe partial stenosis group(c-chd,stenosis development≥75%).The levels of ICAM-1 and LDH in serum of patients were detected by ELISA,and the differences of ICAM-1 and LDH activities in different degrees of coronary artery disease and control group were statistically analyzed.Results:in the control group,HDL was higher than that in the c-chd group(P<0.05),neutrophils in the control group was lower than that in the c-chd group(P<0.05);ICAM-1,LDH and Gensini scores in the a-chd group,b-chd group and c-chd group were higher than those in the control group(P<0.05-P<0.01),and gradually increased with the aggravation of different coronary artery stenosis processes(P<0.05-P<0.01).The levels of serum ICAM-1 and LDH increased significantly with the increase of the number of CHD lesions(P<0.01).The levels of ICAM-1 and LDH were positively correlated with Gensini score(LDH r=0.409,P<0.01 and ICAM-1 r=0.463,P<0.01).The levels of ICAM-1 and neutrophils were positively correlated(r=0.208,P<0.01).The levels of LDH and ICAM-1 were negatively correlated with HDL(LDH r=-0.184,P<0.05 and ICAM-1)There was a negative correlation between LDH and total protein(r=-0.218,P<0.01),and a positive correlation between ICAM-1 and LDH(r=0.441,P<0.01).Conclusion:the levels of serum ICAM-1 and LDH gradually increase with the aggravation of different coronary artery stenosis,and there is a certain linear relationship between them.
基金National Natural Science Foundation of China(No.81970313)Key Natural Science Project of Bengbu Medical College(No.2020byzd072)Bengbu Medical College Natural Science Key Project(No.2020byzd109)。
文摘Objective:To analyze the correlation between serum Nrf2 and GPX4 activity levels with coronary heart disease(CHD)and the severity of coronary artery disease,and to explore the role of ferroptosis mediated by its signaling pathway in the progression of CHD.Methods:A total of 540 patients suspected of CHD were selected for coronary angiography,of which 360 patients were diagnosed with CHD.The activity levels of Nrf2 and GPX4 in the serum of CHD patients were detected by ELISA,and the differences in the activities of Nrf2 and GPX4 in the presence or absence of CHD were statistically analyzed.Western blot detection of Nrf2 protein expression of peripheral blood mononuclear cells(PBMCS)in the control(CON)and CHD groups(90 cases each).The expression and significance of its signaling pathway in CHD patients were analyzed.Results:The activity levels of Nrf2 and GPX4 in the CHD group were lower than those in the CON group(P<0.05),and the expression levels of Nrf2 in PBMCs of the two groups were detected by Western blot.The protein expression level of Nrf2 in the CHD group(0.25±0.05)was down‑regulated compared with CON group(0.87±0.16)(P<0.05),indicating that Nrf2 protein expression level was low in CHD patients.Pearson correlation analysis showed that serum Nrf2 and GPX4 levels were negatively correlated with Gensini score(Nrf2:r=‑0.347,P<0.001;GPX4:r=-0.423,P=0.001).Nrf2 and GPX4 were negatively correlated with TG(Nrf2:r=-0.284,P<0.001;GPX4:r=-0.275,P=0.001),Nrf2 and GPX4 levels were negatively correlated with LDL(Nrf2:r=-0.418,P<0.001)0.05;(GPX4:r=-0.426,P<0.05),Nrf2 and GPX4 levels were positively correlated with HDL(Nrf2:r=0.318,P<0.05;GPX4:r=0.428,P<0.05),and Nrf2 was positively correlated with GPX4(r=0.456,P<0.01).Conclusion:The ferroptosis pathway mediated by the Nrf2‑GPX4 signaling pathway is closely related to the degree of coronary artery disease and the pathogenesis of CHD,and its mechanism may be related to the down‑regulation of the Nrf2‑GPX4 signaling pathway.
文摘Background Angina pectoris has been recognized as one of the principal symptoms of aortic valve stenosis （AS）,even in patients without significant coronary artery disease （CAD）.However,the incidence of angina pectoris and related CAD in such patients is controversial.There is continuing debate as to whether coronary angiography is necessary before aortic valve replacement （AVR） in patients with severe AS.The purpose of this study was to evaluate the incidence and predictors of CAD in patients with severe AS in a Korean population.Methods Data from all consecutive patients with severe AS undergoing AVR at a major tertiary cardiac and vascular center in Korea were entered in a prospective registry beginning in 1995.Clinical and echocardiographic follow-up data were recorded into the database annually.Significant CAD was defined as one or more major coronary arteries having an estimated narrowing of ≥70％ and left main coronary arteries having an estimated narrowing of ≥50％ on coronary angiography.We excluded patients with multiple valve disease,significant aortic regurgitation,or prior CAD or valve surgery.Results Totally 574 patients with severe AS （mean age,（65.9±9.6） years） were enrolled in this study.Significant CAD was found in 61 patients （10.6％）.Factors associated with increased likelihood of CAD were age,hypertension,diabetes mellitus,chronic renal failure,carotid disease,and aorta calcification.In Logistic regression analysis,the independent predictor of the presence of CAD was age （P=0.011）.The incidence of CAD increased significantly at 69.2 years of age.Having two risk factors for cardiovascular disease was the most useful cutoff to predict whether a patient was going to have significant CAD.Conclusions There was a low incidence of significant CAD in a population of Korean patients with severe AS.Therefore,coronary angiography before AVR will be considered in patients with multiple risk factors for cardiovascular disease or in patients more than 69 years of age without risk factors for cardiovascular disease.
