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Dexmedetomidine Alleviates Pulmonary Edema by Upregulating AQP1 and AQP5 Expression in Rats with Acute Lung Injury Induced by Lipopolysaccharide 被引量:30
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作者 姜远旭 戴中亮 +3 位作者 张雪萍 赵伟 黄强 高利昆 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2015年第5期684-688,共5页
This study aims to elucidate the mechanisms by which dexmedetomidine alleviates pulmonary edema in rats with acute lung injury induced by lipopolysaccharide (LPS). Male Wistar rats were randomly divided into five gr... This study aims to elucidate the mechanisms by which dexmedetomidine alleviates pulmonary edema in rats with acute lung injury induced by lipopolysaccharide (LPS). Male Wistar rats were randomly divided into five groups: normal saline control (NS) group, receiving intravenous 0.9% normal saline (5 mL/kg); LPS group, receiving intravenous LPS (10 mg/kg); small-dose dexmedetomidine (S) group, treated with a small dose of dexmedetomidine (0.5 μg·kg^-1·h^-1); medium-dose dexmedetomidine (M) group, treated with a medium dose of dexmedetomidine (2.5 μg·kg^-1·h^-1); high-dose dexmedetomidine (H) group, treated with a high dose of dexmedetomidine (5μg·kg^-1·h^-1). The rats were sacrificed 6 h after intravenous injection of LPS or NS, and the hmgs were removed for evaluating histological characteristics and determining the lung wet/dry weight ratio (W/D). The levels of tumor necrosis factor-alpha (TNF-α) and interleukin-1β (IL-1β) in the lung tissues were assessed by enzyme-linked immunosorbent assay (ELISA). The mRNA and protein expression levels of aquaporin-1 (AQP1) and aquaporin-5 (AQP5) were detected by RT-PCR, immunohistochemistry, and Western blot- ting. The lung tissues from the LPS groups were significantly damaged, which were less pronounced in the H group but not in the small-dose dexmedetomidine group or medium-dose dexmedetomidine group. The W/D and the concentrations of TNF-α and IL-1β in the pulmonary tissues were increased in the LPS group as compared with those in NS group, which were reduced in the H group but not in S group or M group (P〈0.01). The expression of AQP1 and AQP5 was lower in the LPS group than in the NS group, and significantly increased in the H group but not in the S group or M group (P〈0.01). Our findings suggest that dexmedetomidine may alleviate pulmonary edema by increasing the expression of AQP-1 and AQP-5. 展开更多
关键词 DEXMEDETOMIDINE acute lung injury lung edema aquaporin-1 aquaporin-5
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脱氢穿心莲内酯琥珀酸半酯上调水通道蛋白5减轻LPS诱导的急性肺损伤肺水肿的实验研究 被引量:11
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作者 朱涛 肖敏 +1 位作者 文富强 汪涛 《西部医学》 2014年第1期16-18,22,共4页
目的探讨脱氢穿心莲内酯琥珀酸半酯(DAS)对于急性肺损伤状态下水通道蛋白5(AQP-5)的调节作用及对肺水肿清除的影响。方法 30只雄性BALB/C小鼠平均分为对照组(Control组)、治疗组(LPS+DAS组)和内毒素组(LPS模型组),每组10只。通过HE染色... 目的探讨脱氢穿心莲内酯琥珀酸半酯(DAS)对于急性肺损伤状态下水通道蛋白5(AQP-5)的调节作用及对肺水肿清除的影响。方法 30只雄性BALB/C小鼠平均分为对照组(Control组)、治疗组(LPS+DAS组)和内毒素组(LPS模型组),每组10只。通过HE染色观察肺组织病理学改变;使用湿/干比(W/D)分析肺水肿程度;使用RTPCR和western blotting对AQP-5的表达进行测量。结果小鼠气道内注射内毒素(LPS)72小时后肺损伤评分和W/D比值明显增加,AQP-5表达量水平明显下降,差异有显著统计学意义(均P<0.05)。同时发现,LPS+DAS组小鼠肺损伤评分及W/D比值较LPS组较低,而AQP-5表达量较之升高,差异均有统计学意义(均P<0.05)。结论脱氢穿心莲内酯琥珀酸半酯可以有效减轻LPS诱导的小鼠ALI导致的肺水肿,其机制可能与上调了AQP-5的表达相关。该结果为脱氢穿心莲内酯琥珀酸半酯治疗ALI/ARDS相关的肺水肿奠定了实验基础。 展开更多
关键词 脱氢穿心莲内酯琥珀酸半酯 急性肺损伤 水通道蛋白5 NF-KB
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