Objective: To study the correlation of apoptosis inducer TWEAK and extracellular matrix protein Del-1 with pancreatic cancer malignancy. Methods: A total of 54 patients with pancreatic cancer who received radical rese...Objective: To study the correlation of apoptosis inducer TWEAK and extracellular matrix protein Del-1 with pancreatic cancer malignancy. Methods: A total of 54 patients with pancreatic cancer who received radical resection and 32 patients with pancreatic trauma who received partial resection in Tianyou Hospital Affiliated to Wuhan University of Science &Technology between May 2014 and March 2017 were selected, proper amount of pancreatic cancer tissue and normal pancreatic tissue were collected respectively, RNA was extracted and synthesized into cDNA by reverse transcription, and then fluorescence quantitative PCR kits were used to determine TWEAK, Del-1, Survivin, USP9X, DPF2, Stathmin, CD44v9, GPSM2, MMP9, Snail and Vimentin mRNA expression. Results: TWEAK, Del-1, Survivin, USP9X, DPF2, Stathmin, CD44v9, GPSM2, MMP9, Snail and Vimentin mRNA expression in pancreatic cancer tissue were significantly higher than those in normal pancreatic tissue;Survivin, USP9X, DPF2 and Stathmin mRNA expression in pancreatic cancer tissue with high TWEAK expression were significantly higher than those in pancreatic cancer tissue with low TWEAK expression;CD44v9, GPSM2, MMP9, Snail and Vimentin mRNA expression in pancreatic cancer tissue with high Del-1 expression were significantly higher than those in pancreatic cancer tissue with low Del-1 expression. Conclusion: The high expression of TWEAK and Del-1 in pancreatic cancer tissue can promote the proliferation and invasion of pancreatic cancer cells respectively.展开更多
应用移动1/N展开方法,计算Van del pol振子方程的本征值。对v=0,且应用SUSY—QM计算其本征值。当光泵参数g远低于阈值或在阈值附近,所有本征值都能和数值计算结果相吻合。当g远高于阈值时,基态和第一激发态本征值亦能和数值计算结果相...应用移动1/N展开方法,计算Van del pol振子方程的本征值。对v=0,且应用SUSY—QM计算其本征值。当光泵参数g远低于阈值或在阈值附近,所有本征值都能和数值计算结果相吻合。当g远高于阈值时,基态和第一激发态本征值亦能和数值计算结果相吻合。展开更多
AIM To investigate the association of NFKB1 gene-94 ATTG insertion/deletion(rs28362491) polymorphism with inflammatory markers and risk of diabetic nephropathy in Asian Indians.METHODS A total of 300 subjects were rec...AIM To investigate the association of NFKB1 gene-94 ATTG insertion/deletion(rs28362491) polymorphism with inflammatory markers and risk of diabetic nephropathy in Asian Indians.METHODS A total of 300 subjects were recruited(100 each), normoglycemic,(NG); type 2 diabetes mellitus(T2DM) without any complications(DM) and T2 DM with diabetic nephropathy [DM-chronic renal disease(CRD)]. Analysis was carried out by polymerase chain reaction-restriction fragment length polymorphism and ELISA. Pearson's correlation, analysis of variance and logistic regression wereused for statistical analysis.RESULTS The allelic frequencies of-94 ATTG insertion/deletion were 0.655/0.345(NG), 0.62/0.38(DM) and 0.775/0.225(DM-CRD). The-94 ATTG ins allele was associated with significantly increased levels of urinary monocyte chemoattractant protein-1(u MCP-1); u MCP-1(P = 0.026) and plasma tumor necrosis factor-alpha(TNF-α); TNF-α(P = 0.030) and almost doubled the risk of diabetic nephropathy(OR = 1.91, 95%CI: 1.080-3.386, P = 0.025).CONCLUSION-94 ATTG ins/ins polymorphism might be associated with increased risk of developing nephropathy in Asian Indian subjects with diabetes mellitus.展开更多
文摘Objective: To study the correlation of apoptosis inducer TWEAK and extracellular matrix protein Del-1 with pancreatic cancer malignancy. Methods: A total of 54 patients with pancreatic cancer who received radical resection and 32 patients with pancreatic trauma who received partial resection in Tianyou Hospital Affiliated to Wuhan University of Science &Technology between May 2014 and March 2017 were selected, proper amount of pancreatic cancer tissue and normal pancreatic tissue were collected respectively, RNA was extracted and synthesized into cDNA by reverse transcription, and then fluorescence quantitative PCR kits were used to determine TWEAK, Del-1, Survivin, USP9X, DPF2, Stathmin, CD44v9, GPSM2, MMP9, Snail and Vimentin mRNA expression. Results: TWEAK, Del-1, Survivin, USP9X, DPF2, Stathmin, CD44v9, GPSM2, MMP9, Snail and Vimentin mRNA expression in pancreatic cancer tissue were significantly higher than those in normal pancreatic tissue;Survivin, USP9X, DPF2 and Stathmin mRNA expression in pancreatic cancer tissue with high TWEAK expression were significantly higher than those in pancreatic cancer tissue with low TWEAK expression;CD44v9, GPSM2, MMP9, Snail and Vimentin mRNA expression in pancreatic cancer tissue with high Del-1 expression were significantly higher than those in pancreatic cancer tissue with low Del-1 expression. Conclusion: The high expression of TWEAK and Del-1 in pancreatic cancer tissue can promote the proliferation and invasion of pancreatic cancer cells respectively.
基金supported by Indian Council of Medical Research and Postgraduate Research Grant, University College of Medical Sciences, New Delhi
文摘AIM To investigate the association of NFKB1 gene-94 ATTG insertion/deletion(rs28362491) polymorphism with inflammatory markers and risk of diabetic nephropathy in Asian Indians.METHODS A total of 300 subjects were recruited(100 each), normoglycemic,(NG); type 2 diabetes mellitus(T2DM) without any complications(DM) and T2 DM with diabetic nephropathy [DM-chronic renal disease(CRD)]. Analysis was carried out by polymerase chain reaction-restriction fragment length polymorphism and ELISA. Pearson's correlation, analysis of variance and logistic regression wereused for statistical analysis.RESULTS The allelic frequencies of-94 ATTG insertion/deletion were 0.655/0.345(NG), 0.62/0.38(DM) and 0.775/0.225(DM-CRD). The-94 ATTG ins allele was associated with significantly increased levels of urinary monocyte chemoattractant protein-1(u MCP-1); u MCP-1(P = 0.026) and plasma tumor necrosis factor-alpha(TNF-α); TNF-α(P = 0.030) and almost doubled the risk of diabetic nephropathy(OR = 1.91, 95%CI: 1.080-3.386, P = 0.025).CONCLUSION-94 ATTG ins/ins polymorphism might be associated with increased risk of developing nephropathy in Asian Indian subjects with diabetes mellitus.
