Distinguishing between nonalcoholic steatohepatitis(NASH) and advanced liver fibrosis is the key for clinical diagnosis of non-alcoholic fatty liver disease(NAFLD). Liver biopsy, which is widely used for diagnosis of ...Distinguishing between nonalcoholic steatohepatitis(NASH) and advanced liver fibrosis is the key for clinical diagnosis of non-alcoholic fatty liver disease(NAFLD). Liver biopsy, which is widely used for diagnosis of liver diseases at present, has many drawbacks, such as being invasive, expensive and unstable. This article compares and summarizes the commonly used non-invasive diagnostic methods, including their diagnostic parameters, advantages and disadvantages, in order to provide a useful reference for the diagnosis of NASH.展开更多
Chinese medicine(CM)has thousands of years of experience in prevention of diseases.As for CM,people's constitution is closely related to their health status,thus recognition of CM constitution is the fundamental a...Chinese medicine(CM)has thousands of years of experience in prevention of diseases.As for CM,people's constitution is closely related to their health status,thus recognition of CM constitution is the fundamental and core contentof research on constitution types.With development of technologies such as sensors,arificial intelligence and big data,objectification of the four diagnostic methods of CM has gradually matured,bringing changes in the mindset and innovations in technical means for recognition of CM constitution.This paper presents a systematic review of the latest research trends in constitution recognition based on objectification of diagnostic methods in CM.展开更多
Background:This study was undertaken to retrospectively analyze the accuracy of different methods in differentiating biliary atresia from neonatal jaundice.Methods:A search was made in MEDLINE,and the Web of Science f...Background:This study was undertaken to retrospectively analyze the accuracy of different methods in differentiating biliary atresia from neonatal jaundice.Methods:A search was made in MEDLINE,and the Web of Science for relevant original articles published in English;methodological quality of the included studies was also assessed.Two reviewers extracted data independently.Studies were pooled,summary receiver operating characteristics curve and diagnostic odds ratio(DOR)with corresponding confi dence intervals were calculated.Results:For diagnosis of biliary atresia,ultrasonography(US),hepatic scintigraphy(HBS),and magnetic resonance cholangiography(MRCP)had a pooled sensitivity of 74.9%(range:70.4%-79.1%),93.4%(range:90.3%-95.7%)and 89.7%(range:84.8%-93.4%),a specificity of 93.4%(range:91.4%-95.1%),69.2%(range:65.1%-73.1%)and 64.7%(range:58.0%-71.0%),a positive likelihood ratio of 12.16(range:6.41-23.08),3.01(range:2.15-4.20)and 3.10(range:1.59-6.06),a negative likelihood ratio of 0.23(range:0.13-0.38),0.13(range:0.06-0.25)and 0.16(range:0.06-0.44),DOR of 72.56(range:27.34-192.58),29.88(range:12.82-69.64)and 32.48(range:8.22-128.29),with an area under the curve of 0.96,0.91,and 0.92,and Q value of 0.90,0.85,and 0.85,respectively.Conclusions:US,HBS and MRCP can be very useful for the diagnostic work-up of neonatal cholestasis.To improve the sensitivity and specificity,several additional measures can be used.展开更多
Cardioembolic stroke,referred to as cardiogenic stroke,is a clinical syndrome in which emboli from the heart pass through the circulatory system and cause cerebral artery embolism and corresponding brain dysfunction.C...Cardioembolic stroke,referred to as cardiogenic stroke,is a clinical syndrome in which emboli from the heart pass through the circulatory system and cause cerebral artery embolism and corresponding brain dysfunction.Compared to other subtypes of ischemic stroke,cardiogenic stroke presents with more etiologies,greater severity,worse prognosis,and a higher recurrence rate.In this minireview,we provide new insights into the etiological classification,diagnostic methods,and interventions of cardiogenic stroke.展开更多
Transplutonium isotopes are scarce and need to be produced by irradiation in high flux reactors.However,their production is inefficient,and optimization studies are necessary.This study analyzes the physical nature of...Transplutonium isotopes are scarce and need to be produced by irradiation in high flux reactors.However,their production is inefficient,and optimization studies are necessary.This study analyzes the physical nature of transplutonium isotope produc-tion using ^(252)Cf,^(244)Cm,^(242)Cm,and ^(238)Pu as examples.Traditional methods based on the Monte Carlo burnup calculation have the limitations of many calculations and cannot analyze the individual energy intervals in detail;thus,they cannot sup-port the refined evaluation,screening,and optimization of the irradiation schemes.After understanding the physical nature and simplifying the complexity of the production process,we propose a rapid diagnostic method for evaluating radiation schemes based on the concepts“single energy interval value(SEIV)”and“energy spectrum total value(ESTV)”.The rapid diagnostic method not only avoids tedious burnup calculations,but also provides a direction for optimization.The optimal irradiation schemes for producing ^(252)Cf,^(244)Cm,^(242)Cm,and ^(238)Pu are determined based on a rapid diagnostic method.Optimal irradiation schemes can significantly improve production efficiency.Compared with the initial scheme,the optimal scheme improved the production efficiency of ^(238)Pu by 7.41 times;^(242)Cm,11.98 times;^(244)Cm,65.20 times;and ^(252)Cf,15.08 times.Thus,a refined analysis of transplutonium isotope production is conducted and provides a theoretical basis for improving production efficiency.展开更多
In this editorial we comment on the article published by Ning et al,“Role of exosomes in metastasis and therapeutic resistance in esophageal cancer”.Esophageal cancer(EC)represents a significant global health concer...In this editorial we comment on the article published by Ning et al,“Role of exosomes in metastasis and therapeutic resistance in esophageal cancer”.Esophageal cancer(EC)represents a significant global health concern,being the seventh most common and sixth in terms of mortality worldwide.Despite the advances in therapeutic modalities,the management of patients with EC remains challenging,with a 5-year survival rate of only 25%and a limited eligibility for curative surgery due to its late diagnosis.Conventional screening methods are impractical for the early detection of EC,given their either invasive or insensitive nature.The advent of liquid biopsy,with a focus on circulating tumor cells,circulating tumor DNA,and exosomes,heralds a non-invasive avenue for cancer detection.Exosomes,small vesicles involved in intercellular communication,are highlighted as potential biomarkers for EC diagnosis and prognosis.Along with a diverse cargo encompassing various types of RNA,DNA molecules,proteins,and metabolites,exosomes emerge as key players in tumorigenesis,tumor development,and metastasis.Their significance extends to carrying distinctive biomarkers,including microRNAs(miRNAs),long non-coding RNAs,and circular RNAs,underscoring their potential diagnostic and prognostic value.Furthermore,exosomes may be utilized for therapeutic purposes in the context of EC treatment,serving as efficient delivery vehicles for therapeutic agents such as chemotherapeutic medicines and miRNAs.In this editorial we delve into the applications of exosomes for the early detection and treatment of EC,as well as the future perspectives.展开更多
Our national center of gastroenterology provides highly specialized care, including chronic pancreatitis. Another area of our activities is educational programs, including postgraduate and fellows’ courses. Thereby, ...Our national center of gastroenterology provides highly specialized care, including chronic pancreatitis. Another area of our activities is educational programs, including postgraduate and fellows’ courses. Thereby, we have noted significant gaps in the knowledge of the specialists that involved in the chronic pancreatitis management. The most critical downsides are related to insufficient attention to etiology and risk factors, using outdated classifications, the lack of knowledge in arsenal of diagnostic techniques, polypharmacy or application of low level of evidence treatment methods. Finally, we have made amendments in the National Clinical Protocol in Chronic Pancreatitis [<a href="#ref1">1</a>], updated the State Educational Standard for residents of the gastroenterological profile [<a href="#ref2">2</a>]. The aim of the study was the analysis of the basic knowledge among different specialists in the management of chronic pancreatitis (CP) around the country. This qualitative study consists of two parts, including focus group interviews followed by interviews with the specialists across the country, which was conducted during the period 2015-2018. In this paper, we present results of diagnostic approaches at the different levels of medical care. The general practitioners have noted the absence of modern methods of laboratory and visual diagnostics in their routine practice, therefore explaining the plenty of the complicated forms. Another issue is the low specialist’s adherence to clinical guidelines, poor knowledge of the risk factors and overestimation of the clinical presentation value except malnutrition symptoms. On the other hand, surgeons and other specialists are not ready to implement modern diagnostic tools and methods in their practice. Obviously, in accordance with the results of our study, our educational center should conduct a number of training activities, as well as develop new algorithms for medical care specialists.展开更多
An attempt has been made in the present study to forecast fog with a diagnostic method using the outputs of global NWP model.The diagnostic method is based on the combination of thresholds of meteorological variables ...An attempt has been made in the present study to forecast fog with a diagnostic method using the outputs of global NWP model.The diagnostic method is based on the combination of thresholds of meteorological variables involved in fog formation.The thresholds are computed using the observations during fog.These thresholds are applied to the output of a global NWP model for forecasting fog.The occurrence of fog is a common phenomenon during winter season over the northern plains of India.The diagnostic method is used to predict fog occurrences over three stations in north India.The proposed method is able to predict both occurrences and non-occurrences of fog at all the three stations.It is found that 94%of the fog events forecasted by the model using the diagnostic method have been actually observed at the selected stations.The performance of method in predicting fog is found best over Delhi with the highest accuracy(0.61)and probability of detection(0.60).The study signifies that diagnostic approach based on the output of a global model is a useful tool for predicting fog over a single location.展开更多
Hand,foot and mouth disease(HFMD)is a major public health problem among children in the Asia-Pacific region.The optimal specimen for HFMD virological diagnosis remains unclear.Enterovirus A71(EV-A71)neutralizing antib...Hand,foot and mouth disease(HFMD)is a major public health problem among children in the Asia-Pacific region.The optimal specimen for HFMD virological diagnosis remains unclear.Enterovirus A71(EV-A71)neutralizing antibody titres detected in paired sera were considered the reference standard for calculating the sensitivity,specificity,positive and negative predictive value of throat swabs,rectal swabs,stool,blood samples and cerebrospinal fluid(CSF)by RT-PCR or ELISA assay.In this study,clinical samples from 276 HFMD patients were collected for analysing the sensitivity of different kind of specimens.Our results showed that stool had the highest sensitivity(88%,95%CI:74%–96%)and agreement with the reference standard(91%).The order of diagnostic yield for EV-A71 infection was stool samplerectal swab>throat swab>blood sample>CSF sample,and using a combination of clinical samples improved sensitivity for enterovirus detection.The sensitivity of ELISA for IgM antibody detection in sterile-site specimens was significantly higher than that of RT-PCR(serum/plasma:62%vs.2%,CSF:47%vs.0%)(P<0.002).In conclusion,our results suggest that stool has the highest diagnostic yield for EV-A71-infected HFMD.If stool is unavailable,rectal swabs can be collected to achieve a similar diagnostic yield.Otherwise,throat swabs may be useful in detecting positive samples.Although IgM in blood or CSF is diagnostically accurate,it lacks sensitivity,missing 40%–50%of cases.The higher proportion of severe cases and shorter interval between onset and sampling contributed to the increase in congruency between clinical testing and the serological reference standard.展开更多
The characteristics of chicken colibacillosis are introduced from the aspects of pathogen,epidemiology,clinical symptoms and necropsy,and clinical comprehensive diagnosis and laboratory diagnosis methods are given.The...The characteristics of chicken colibacillosis are introduced from the aspects of pathogen,epidemiology,clinical symptoms and necropsy,and clinical comprehensive diagnosis and laboratory diagnosis methods are given.The disease is distinguished from diseases with similar symptoms such as chicken chlamydia,chicken streptococcus,chicken necrotic enteritis,broiler ascites syndrome and chicken paratyphoid infections.Eventually,the preventive and therapeutic measures against the disease are put forward.展开更多
The characteristics of duck virus enteritis(DVE)are introduced from the aspects of pathogen,epidemiology,clinical symptoms,autopsy pathological changes,etc.,and clinical comprehensive diagnosis and laboratory diagnosi...The characteristics of duck virus enteritis(DVE)are introduced from the aspects of pathogen,epidemiology,clinical symptoms,autopsy pathological changes,etc.,and clinical comprehensive diagnosis and laboratory diagnosis methods are given.The disease is distinguished from similar diseases including duck necrotic enteritis,duck candidiasis,duck hemorrhage,duck coccidiosis and duck vitamin A deficiency.Eventually,the prevention and treatment measures of the disease are put forward.展开更多
The Jing-well point temperatures test method is a method to diagnose and guide the treatment of diseases by measuring the subjects' symmetrical well point temperature. it is improved from the method of knowing hea...The Jing-well point temperatures test method is a method to diagnose and guide the treatment of diseases by measuring the subjects' symmetrical well point temperature. it is improved from the method of knowing heat sensitivity. The application of Jing-well point temperatures test method is wide, and it can be used in internal and external gynecology and pediatrics and facial features department. at the same time, it has the advantage of objective and accurate diagnosis. The old law has some shortcomings, such as poor intuition, unavoidable omission of information, incomplete interpretation of information and so on. In this paper, Excel software is used to transform the data into line chart form, which improves the intuition and comprehensiveness of this method, so that the data can be better interpreted and used. It is newly proposed in this article that in addition to observing the longitudinal di fference of well point temperature, more attention should be paid to the horizontal contrast difference of well point temperature in different meridians. The article also summarizes a number of treatment methods, including acupuncture, moxa moxibustion, cupping and scraping, and the selection of acupoints, including mother acupoints, tenderness points and heat-sensitive moxibustion, so that doctors can combine traditional Chinese medicine professional knowledge in clinic.展开更多
A millimeter wave solid state source—far infrared laser combined interferometer system(MFCI)consisting of a three-channel 890 GHz hydrogen cyanide(HCN)laser interferometer and a threechannel 340 GHz solid state sourc...A millimeter wave solid state source—far infrared laser combined interferometer system(MFCI)consisting of a three-channel 890 GHz hydrogen cyanide(HCN)laser interferometer and a threechannel 340 GHz solid state source interferometer(SSI)is developed for real-time line-integrated electron density feedback and electron density profile of the EXL-50 spherical tokamak device.The interferometer system is a Mach–Zehnder type,with all probe-channels measured vertically,covering the plasma magnetic axis to the outermost closed magnetic plane.The HCN laser interferometer uses an HCN laser with a frequency of 890 GHz as a light source and modulates a100 k Hz beat signal by a rotating grating,giving a temporal resolution of 10μs.The SSI uses two independent 340 GHz solid-state diode sources as the light source,the frequency of the two sources is adjustable,and the temporal resolution of SSI can reach 1μs by setting the frequency difference of the two lasers at 1 MHz.The main optical path of the two interferometers is compactly installed on a set of double-layer optical platform directly below EXL-50.Dual optical path design using corner cube reflectors avoids the large support structures.Collinear the probebeams of two wavelengths,then the phase error caused by vibration can be compensated.At present,the phase noise of the HCN Interferometer is 0.08 rad,corresponding to a line-integrated electron density of 0.88×10^(17)m^(-2),one channel of measuring result was obtained by the MFCI system,and the highest density measured is about 0.7×10^(19)m^(-2).展开更多
Background: Video recording of cells offers a straightforward way to gainvaluable information from their response to treatments. An indispensable stepin obtaining such information involves tracking individual cells fr...Background: Video recording of cells offers a straightforward way to gainvaluable information from their response to treatments. An indispensable stepin obtaining such information involves tracking individual cells from therecorded data. A subsequent step is reducing such data to represent essentialbiological information. This can help to compare various single‐cell trackingdata yielding a novel source of information. The vast array of potential datasources highlights the significance of methodologies prioritizing simplicity,robustness, transparency, affordability, sensor independence, and freedomfrom reliance on specific software or online services.Methods: The provided data presents single‐cell tracking of clonal (A549)cells as they grow in two‐dimensional (2D) monolayers over 94 hours,spanning several cell cycles. The cells are exposed to three differentconcentrations of yessotoxin (YTX). The data treatments showcase theparametrization of population growth curves, as well as other statisticaldescriptions. These include the temporal development of cell speed in familytrees with and without cell death, correlations between sister cells, single‐cellaverage displacements, and the study of clustering tendencies.Results: Various statistics obtained from single‐cell tracking reveal patternssuitable for data compression and parametrization. These statistics encompassessential aspects such as cell division, movements, and mutual informationbetween sister cells.Conclusion: This work presents practical examples that highlight theabundant potential information within large sets of single‐cell tracking data.Data reduction is crucial in the process of acquiring such information whichcan be relevant for phenotypic drug discovery and therapeutics, extendingbeyond standardized procedures. Conducting meaningful big data analysistypically necessitates a substantial amount of data, which can stem fromstandalone case studies as an initial foundation.展开更多
Background:Early neurologic deterioration(END)may occur in patients with anterior circulation ischemic stroke(ACIS)after receiving endovascular treatment(EVT).Hemodynamic insufficiency,re-occlusion,and post-re-canaliz...Background:Early neurologic deterioration(END)may occur in patients with anterior circulation ischemic stroke(ACIS)after receiving endovascular treatment(EVT).Hemodynamic insufficiency,re-occlusion,and post-re-canalization hyper-perfusion are likely to play a critical role in END.We hypothesized that hemodynamic changes can predict END in patients with ACIS postsuccessful EVT using trans-cranial Doppler(TCD).Methods:We utilized a prospectively maintained database of ACIS patients treated with EVT between September 2016 and June 2018 in the Xuanwu Hospital,Capital Medical University.TCD parameters including peak systolic velocity(PSV),bilateral mean flow velocity(MFV),and pulse index(PI)were determined via the middle cerebral arteries within 72 h post-EVT.A logistic regression model was applied to detect independent predictors for END.Results:Totally,112 EVT patients were included in this study and 80/112 patients experienced successful re-canalization with<50%residual stenosis,while 17/80(21.3%)patients suffered END,for which vasogenic cerebral edema(11/17)was considered as a leading role and followed by symptomatic intra-cranial hemorrhage(4/17)and ischemia progression(2/17).For the 80 patients,the PSV(median:127 cm/s vs.116 cm/s,P=0.039),the ratio of ipsilateral-MFV/contra-lateral-MFV(iMFV/cMFV)(median:1.29 vs.1.02,P=0.036)and iMFV/mean blood pressure(MBP)(median:0.97 vs.0.79,P=0.008)in END patients were higher than those of non-END.Using the receiver-operating characteristic curve to obtain cut-off values for PSV,PI,iMFV/cMFV,and iMFV/MBP for END,we found that PI≥0.85(odds ratio:11.03,95%confidence interval:1.92–63.46,P=0.007)and iMFV/MBP≥0.84(odds ratio:9.20,95%confidence interval:2.07–40.84,P=0.004)were independent predictors of END in a multivariate logistic regression model,with a sensitivity of 82.4%and 76.5%and a specificity of 42.9%and 66.7%,respectively,and had the positive predictive values of 29.0%and 38.2%,and negative predictive values of 90.0%and 91.3%,with an area under the receiveroperating characteristic curve of 0.57 and 0.71,respectively.Conclusion:TCD examination of EVT patients may be used as a real-time tool to detect END predictors,such as the higher PI and iMFV/MBP,allowing for better post-thrombectomy management in ACIS patients.展开更多
Traditional Chinese medicine(TCM)is gaining popularity in the treatment of psychiatric disorders[1,2]that can be described and treated from either an Eastern or a Western perspective.In Eastern medicine,the disorders ...Traditional Chinese medicine(TCM)is gaining popularity in the treatment of psychiatric disorders[1,2]that can be described and treated from either an Eastern or a Western perspective.In Eastern medicine,the disorders are described according to fi ve diagnostic methods that展开更多
Aquaculture provides significant contributions to household food security,as the capture of wild fish from lakes,dams,and oceans do not meet the current demand for animal protein in Tanzania.Sustainable aquaculture re...Aquaculture provides significant contributions to household food security,as the capture of wild fish from lakes,dams,and oceans do not meet the current demand for animal protein in Tanzania.Sustainable aquaculture requires well-established regulatory systems and extension services for good pond management practices and maintaining fish health by fish farmers.Fish farming is practiced widely in Tanzania,from small-to large-scale ponds and these farming systems are moving from extensive normal operations(low input demand)to intensive farming(high input demand).However,the industry is largely still operating at a subsistence level with low production.Bacterial infections have been occurring in these fish farms and will continue to be an issue of concern into the future.This review highlights the current challenges,successes,and prospects towards a sustainable aquaculture industry in Tanzania,including:limited extension services mirroring the limited knowledge by farmers regarding pond management practices;the inadequacy of funds to carry out fish disease research or implement a surveillance system;little expertise in fish disease diagnosis and treatment;and poor management options.To minimize disease outbreaks and optimize production in the future,we suggest a strengthening of extension services,augmented with on-farm knowledge transfer.Emphasis should be on pond management practices and fish disease management;the creation of a well-functioning fish disease surveillance system;and strengthening collaborative research on aquaculture between the government research institutions and academia.Establishing small cooperative fish farmer groups within the Aquaculture Association of Tanzania(AAT)for easy access to information is also recommended.展开更多
We propose a spatial diffraction diagnostic method via inserting a millimeter-gap double slit into the collimated terahertz beam to monitor the minute variation of the terahertz beam in strong-field terahertz sources,...We propose a spatial diffraction diagnostic method via inserting a millimeter-gap double slit into the collimated terahertz beam to monitor the minute variation of the terahertz beam in strong-field terahertz sources,which is difficult to be resolved in conventional terahertz imaging systems.To verify the method,we intentionally fabricate tiny variations of the terahertz beam through tuning the iris for the infrared pumping beam before the tilted-pulse-front pumping setups.The phenomena can be well explained by the theory based on the tilted-pulse-front technique and terahertz diffraction.展开更多
In this study,flowback-assisted minifrac tests were conducted in low-permeability shale and salt formations to measure the in situ stress.An injection/flowback testing protocol was implemented in each test to achieve ...In this study,flowback-assisted minifrac tests were conducted in low-permeability shale and salt formations to measure the in situ stress.An injection/flowback testing protocol was implemented in each test to achieve accuracy and efficiency.Accurate and efficient injection/flowback testing is very important,given the impermeable nature of these formations and the need to complete each test as quickly as possible.Each flowback cycle yields a distinct and repeatable fracture closure signature,simplifying the interpretation of the fracture closure pressure.The objective of this paper is to share our field experience and to present a numerical analysis of the flowback test pressure responses,fracture closure behaviors,and fracture closure diagnostic methods.Examples from open-hole and casedhole minifrac tests are used to demonstrate site operation procedures.Then,two numerical models are presented for simulating the fracture closure behavior during a flowback test.Field evidence is provided to demonstrate that the fracture closure pressures from the flowback tests are identical to those from tests without flowback.The fracture closure diagnostic methods for flowback tests are discussed,and it is found that the G-function diagnostic method yields a distinct fracture closure signal during the flowback tests.This study is intended to provide additional insights regarding flowback tests by sharing our successes,experience,and knowledge,thereby benefiting the industry.展开更多
基金Supported by Traditional Chinese Medicine Development Program of Shandong Province (2021Q097)。
文摘Distinguishing between nonalcoholic steatohepatitis(NASH) and advanced liver fibrosis is the key for clinical diagnosis of non-alcoholic fatty liver disease(NAFLD). Liver biopsy, which is widely used for diagnosis of liver diseases at present, has many drawbacks, such as being invasive, expensive and unstable. This article compares and summarizes the commonly used non-invasive diagnostic methods, including their diagnostic parameters, advantages and disadvantages, in order to provide a useful reference for the diagnosis of NASH.
基金Supported by Hebei Province Key Research and Development Project(No.203777119D)。
文摘Chinese medicine(CM)has thousands of years of experience in prevention of diseases.As for CM,people's constitution is closely related to their health status,thus recognition of CM constitution is the fundamental and core contentof research on constitution types.With development of technologies such as sensors,arificial intelligence and big data,objectification of the four diagnostic methods of CM has gradually matured,bringing changes in the mindset and innovations in technical means for recognition of CM constitution.This paper presents a systematic review of the latest research trends in constitution recognition based on objectification of diagnostic methods in CM.
文摘Background:This study was undertaken to retrospectively analyze the accuracy of different methods in differentiating biliary atresia from neonatal jaundice.Methods:A search was made in MEDLINE,and the Web of Science for relevant original articles published in English;methodological quality of the included studies was also assessed.Two reviewers extracted data independently.Studies were pooled,summary receiver operating characteristics curve and diagnostic odds ratio(DOR)with corresponding confi dence intervals were calculated.Results:For diagnosis of biliary atresia,ultrasonography(US),hepatic scintigraphy(HBS),and magnetic resonance cholangiography(MRCP)had a pooled sensitivity of 74.9%(range:70.4%-79.1%),93.4%(range:90.3%-95.7%)and 89.7%(range:84.8%-93.4%),a specificity of 93.4%(range:91.4%-95.1%),69.2%(range:65.1%-73.1%)and 64.7%(range:58.0%-71.0%),a positive likelihood ratio of 12.16(range:6.41-23.08),3.01(range:2.15-4.20)and 3.10(range:1.59-6.06),a negative likelihood ratio of 0.23(range:0.13-0.38),0.13(range:0.06-0.25)and 0.16(range:0.06-0.44),DOR of 72.56(range:27.34-192.58),29.88(range:12.82-69.64)and 32.48(range:8.22-128.29),with an area under the curve of 0.96,0.91,and 0.92,and Q value of 0.90,0.85,and 0.85,respectively.Conclusions:US,HBS and MRCP can be very useful for the diagnostic work-up of neonatal cholestasis.To improve the sensitivity and specificity,several additional measures can be used.
文摘Cardioembolic stroke,referred to as cardiogenic stroke,is a clinical syndrome in which emboli from the heart pass through the circulatory system and cause cerebral artery embolism and corresponding brain dysfunction.Compared to other subtypes of ischemic stroke,cardiogenic stroke presents with more etiologies,greater severity,worse prognosis,and a higher recurrence rate.In this minireview,we provide new insights into the etiological classification,diagnostic methods,and interventions of cardiogenic stroke.
基金sponsored by Natural Science Foundation of Shanghai (NO.22ZR1431900)Science and Technology on Reactor System Design Technology Laboratory.
文摘Transplutonium isotopes are scarce and need to be produced by irradiation in high flux reactors.However,their production is inefficient,and optimization studies are necessary.This study analyzes the physical nature of transplutonium isotope produc-tion using ^(252)Cf,^(244)Cm,^(242)Cm,and ^(238)Pu as examples.Traditional methods based on the Monte Carlo burnup calculation have the limitations of many calculations and cannot analyze the individual energy intervals in detail;thus,they cannot sup-port the refined evaluation,screening,and optimization of the irradiation schemes.After understanding the physical nature and simplifying the complexity of the production process,we propose a rapid diagnostic method for evaluating radiation schemes based on the concepts“single energy interval value(SEIV)”and“energy spectrum total value(ESTV)”.The rapid diagnostic method not only avoids tedious burnup calculations,but also provides a direction for optimization.The optimal irradiation schemes for producing ^(252)Cf,^(244)Cm,^(242)Cm,and ^(238)Pu are determined based on a rapid diagnostic method.Optimal irradiation schemes can significantly improve production efficiency.Compared with the initial scheme,the optimal scheme improved the production efficiency of ^(238)Pu by 7.41 times;^(242)Cm,11.98 times;^(244)Cm,65.20 times;and ^(252)Cf,15.08 times.Thus,a refined analysis of transplutonium isotope production is conducted and provides a theoretical basis for improving production efficiency.
文摘In this editorial we comment on the article published by Ning et al,“Role of exosomes in metastasis and therapeutic resistance in esophageal cancer”.Esophageal cancer(EC)represents a significant global health concern,being the seventh most common and sixth in terms of mortality worldwide.Despite the advances in therapeutic modalities,the management of patients with EC remains challenging,with a 5-year survival rate of only 25%and a limited eligibility for curative surgery due to its late diagnosis.Conventional screening methods are impractical for the early detection of EC,given their either invasive or insensitive nature.The advent of liquid biopsy,with a focus on circulating tumor cells,circulating tumor DNA,and exosomes,heralds a non-invasive avenue for cancer detection.Exosomes,small vesicles involved in intercellular communication,are highlighted as potential biomarkers for EC diagnosis and prognosis.Along with a diverse cargo encompassing various types of RNA,DNA molecules,proteins,and metabolites,exosomes emerge as key players in tumorigenesis,tumor development,and metastasis.Their significance extends to carrying distinctive biomarkers,including microRNAs(miRNAs),long non-coding RNAs,and circular RNAs,underscoring their potential diagnostic and prognostic value.Furthermore,exosomes may be utilized for therapeutic purposes in the context of EC treatment,serving as efficient delivery vehicles for therapeutic agents such as chemotherapeutic medicines and miRNAs.In this editorial we delve into the applications of exosomes for the early detection and treatment of EC,as well as the future perspectives.
文摘Our national center of gastroenterology provides highly specialized care, including chronic pancreatitis. Another area of our activities is educational programs, including postgraduate and fellows’ courses. Thereby, we have noted significant gaps in the knowledge of the specialists that involved in the chronic pancreatitis management. The most critical downsides are related to insufficient attention to etiology and risk factors, using outdated classifications, the lack of knowledge in arsenal of diagnostic techniques, polypharmacy or application of low level of evidence treatment methods. Finally, we have made amendments in the National Clinical Protocol in Chronic Pancreatitis [<a href="#ref1">1</a>], updated the State Educational Standard for residents of the gastroenterological profile [<a href="#ref2">2</a>]. The aim of the study was the analysis of the basic knowledge among different specialists in the management of chronic pancreatitis (CP) around the country. This qualitative study consists of two parts, including focus group interviews followed by interviews with the specialists across the country, which was conducted during the period 2015-2018. In this paper, we present results of diagnostic approaches at the different levels of medical care. The general practitioners have noted the absence of modern methods of laboratory and visual diagnostics in their routine practice, therefore explaining the plenty of the complicated forms. Another issue is the low specialist’s adherence to clinical guidelines, poor knowledge of the risk factors and overestimation of the clinical presentation value except malnutrition symptoms. On the other hand, surgeons and other specialists are not ready to implement modern diagnostic tools and methods in their practice. Obviously, in accordance with the results of our study, our educational center should conduct a number of training activities, as well as develop new algorithms for medical care specialists.
文摘An attempt has been made in the present study to forecast fog with a diagnostic method using the outputs of global NWP model.The diagnostic method is based on the combination of thresholds of meteorological variables involved in fog formation.The thresholds are computed using the observations during fog.These thresholds are applied to the output of a global NWP model for forecasting fog.The occurrence of fog is a common phenomenon during winter season over the northern plains of India.The diagnostic method is used to predict fog occurrences over three stations in north India.The proposed method is able to predict both occurrences and non-occurrences of fog at all the three stations.It is found that 94%of the fog events forecasted by the model using the diagnostic method have been actually observed at the selected stations.The performance of method in predicting fog is found best over Delhi with the highest accuracy(0.61)and probability of detection(0.60).The study signifies that diagnostic approach based on the output of a global model is a useful tool for predicting fog over a single location.
基金supported by the National Natural Science Fund for Distinguished Young Scholars of China(No.81525023)in whole or in part,by a Wellcome Trust fellowship awarded to LT[205228/Z/16/Z]supported by the National Institute for Health Research Health Protection Research Unit in Emerging and Zoonotic Infections(grant no.NIHR200907)at University of Liverpool in partnership with Public Health England(PHE),in collaboration with Liverpool School of Tropical Medicine and the University of Oxford.LT is based at the University of Liverpool.
文摘Hand,foot and mouth disease(HFMD)is a major public health problem among children in the Asia-Pacific region.The optimal specimen for HFMD virological diagnosis remains unclear.Enterovirus A71(EV-A71)neutralizing antibody titres detected in paired sera were considered the reference standard for calculating the sensitivity,specificity,positive and negative predictive value of throat swabs,rectal swabs,stool,blood samples and cerebrospinal fluid(CSF)by RT-PCR or ELISA assay.In this study,clinical samples from 276 HFMD patients were collected for analysing the sensitivity of different kind of specimens.Our results showed that stool had the highest sensitivity(88%,95%CI:74%–96%)and agreement with the reference standard(91%).The order of diagnostic yield for EV-A71 infection was stool samplerectal swab>throat swab>blood sample>CSF sample,and using a combination of clinical samples improved sensitivity for enterovirus detection.The sensitivity of ELISA for IgM antibody detection in sterile-site specimens was significantly higher than that of RT-PCR(serum/plasma:62%vs.2%,CSF:47%vs.0%)(P<0.002).In conclusion,our results suggest that stool has the highest diagnostic yield for EV-A71-infected HFMD.If stool is unavailable,rectal swabs can be collected to achieve a similar diagnostic yield.Otherwise,throat swabs may be useful in detecting positive samples.Although IgM in blood or CSF is diagnostically accurate,it lacks sensitivity,missing 40%–50%of cases.The higher proportion of severe cases and shorter interval between onset and sampling contributed to the increase in congruency between clinical testing and the serological reference standard.
文摘The characteristics of chicken colibacillosis are introduced from the aspects of pathogen,epidemiology,clinical symptoms and necropsy,and clinical comprehensive diagnosis and laboratory diagnosis methods are given.The disease is distinguished from diseases with similar symptoms such as chicken chlamydia,chicken streptococcus,chicken necrotic enteritis,broiler ascites syndrome and chicken paratyphoid infections.Eventually,the preventive and therapeutic measures against the disease are put forward.
文摘The characteristics of duck virus enteritis(DVE)are introduced from the aspects of pathogen,epidemiology,clinical symptoms,autopsy pathological changes,etc.,and clinical comprehensive diagnosis and laboratory diagnosis methods are given.The disease is distinguished from similar diseases including duck necrotic enteritis,duck candidiasis,duck hemorrhage,duck coccidiosis and duck vitamin A deficiency.Eventually,the prevention and treatment measures of the disease are put forward.
文摘The Jing-well point temperatures test method is a method to diagnose and guide the treatment of diseases by measuring the subjects' symmetrical well point temperature. it is improved from the method of knowing heat sensitivity. The application of Jing-well point temperatures test method is wide, and it can be used in internal and external gynecology and pediatrics and facial features department. at the same time, it has the advantage of objective and accurate diagnosis. The old law has some shortcomings, such as poor intuition, unavoidable omission of information, incomplete interpretation of information and so on. In this paper, Excel software is used to transform the data into line chart form, which improves the intuition and comprehensiveness of this method, so that the data can be better interpreted and used. It is newly proposed in this article that in addition to observing the longitudinal di fference of well point temperature, more attention should be paid to the horizontal contrast difference of well point temperature in different meridians. The article also summarizes a number of treatment methods, including acupuncture, moxa moxibustion, cupping and scraping, and the selection of acupoints, including mother acupoints, tenderness points and heat-sensitive moxibustion, so that doctors can combine traditional Chinese medicine professional knowledge in clinic.
基金supported by the National MCF Energy R&D Program(Nos.2019YFE03040003 and 2017YFE0301205)supported in part by Key Program of Research and Development of Hefei Science Center,CAS(No.2019HSC-KPRD001)partly supported by the Collaborative Research Program of the Research Institute for Applied Mechanics,Kyushu University.
文摘A millimeter wave solid state source—far infrared laser combined interferometer system(MFCI)consisting of a three-channel 890 GHz hydrogen cyanide(HCN)laser interferometer and a threechannel 340 GHz solid state source interferometer(SSI)is developed for real-time line-integrated electron density feedback and electron density profile of the EXL-50 spherical tokamak device.The interferometer system is a Mach–Zehnder type,with all probe-channels measured vertically,covering the plasma magnetic axis to the outermost closed magnetic plane.The HCN laser interferometer uses an HCN laser with a frequency of 890 GHz as a light source and modulates a100 k Hz beat signal by a rotating grating,giving a temporal resolution of 10μs.The SSI uses two independent 340 GHz solid-state diode sources as the light source,the frequency of the two sources is adjustable,and the temporal resolution of SSI can reach 1μs by setting the frequency difference of the two lasers at 1 MHz.The main optical path of the two interferometers is compactly installed on a set of double-layer optical platform directly below EXL-50.Dual optical path design using corner cube reflectors avoids the large support structures.Collinear the probebeams of two wavelengths,then the phase error caused by vibration can be compensated.At present,the phase noise of the HCN Interferometer is 0.08 rad,corresponding to a line-integrated electron density of 0.88×10^(17)m^(-2),one channel of measuring result was obtained by the MFCI system,and the highest density measured is about 0.7×10^(19)m^(-2).
文摘Background: Video recording of cells offers a straightforward way to gainvaluable information from their response to treatments. An indispensable stepin obtaining such information involves tracking individual cells from therecorded data. A subsequent step is reducing such data to represent essentialbiological information. This can help to compare various single‐cell trackingdata yielding a novel source of information. The vast array of potential datasources highlights the significance of methodologies prioritizing simplicity,robustness, transparency, affordability, sensor independence, and freedomfrom reliance on specific software or online services.Methods: The provided data presents single‐cell tracking of clonal (A549)cells as they grow in two‐dimensional (2D) monolayers over 94 hours,spanning several cell cycles. The cells are exposed to three differentconcentrations of yessotoxin (YTX). The data treatments showcase theparametrization of population growth curves, as well as other statisticaldescriptions. These include the temporal development of cell speed in familytrees with and without cell death, correlations between sister cells, single‐cellaverage displacements, and the study of clustering tendencies.Results: Various statistics obtained from single‐cell tracking reveal patternssuitable for data compression and parametrization. These statistics encompassessential aspects such as cell division, movements, and mutual informationbetween sister cells.Conclusion: This work presents practical examples that highlight theabundant potential information within large sets of single‐cell tracking data.Data reduction is crucial in the process of acquiring such information whichcan be relevant for phenotypic drug discovery and therapeutics, extendingbeyond standardized procedures. Conducting meaningful big data analysistypically necessitates a substantial amount of data, which can stem fromstandalone case studies as an initial foundation.
文摘Background:Early neurologic deterioration(END)may occur in patients with anterior circulation ischemic stroke(ACIS)after receiving endovascular treatment(EVT).Hemodynamic insufficiency,re-occlusion,and post-re-canalization hyper-perfusion are likely to play a critical role in END.We hypothesized that hemodynamic changes can predict END in patients with ACIS postsuccessful EVT using trans-cranial Doppler(TCD).Methods:We utilized a prospectively maintained database of ACIS patients treated with EVT between September 2016 and June 2018 in the Xuanwu Hospital,Capital Medical University.TCD parameters including peak systolic velocity(PSV),bilateral mean flow velocity(MFV),and pulse index(PI)were determined via the middle cerebral arteries within 72 h post-EVT.A logistic regression model was applied to detect independent predictors for END.Results:Totally,112 EVT patients were included in this study and 80/112 patients experienced successful re-canalization with<50%residual stenosis,while 17/80(21.3%)patients suffered END,for which vasogenic cerebral edema(11/17)was considered as a leading role and followed by symptomatic intra-cranial hemorrhage(4/17)and ischemia progression(2/17).For the 80 patients,the PSV(median:127 cm/s vs.116 cm/s,P=0.039),the ratio of ipsilateral-MFV/contra-lateral-MFV(iMFV/cMFV)(median:1.29 vs.1.02,P=0.036)and iMFV/mean blood pressure(MBP)(median:0.97 vs.0.79,P=0.008)in END patients were higher than those of non-END.Using the receiver-operating characteristic curve to obtain cut-off values for PSV,PI,iMFV/cMFV,and iMFV/MBP for END,we found that PI≥0.85(odds ratio:11.03,95%confidence interval:1.92–63.46,P=0.007)and iMFV/MBP≥0.84(odds ratio:9.20,95%confidence interval:2.07–40.84,P=0.004)were independent predictors of END in a multivariate logistic regression model,with a sensitivity of 82.4%and 76.5%and a specificity of 42.9%and 66.7%,respectively,and had the positive predictive values of 29.0%and 38.2%,and negative predictive values of 90.0%and 91.3%,with an area under the receiveroperating characteristic curve of 0.57 and 0.71,respectively.Conclusion:TCD examination of EVT patients may be used as a real-time tool to detect END predictors,such as the higher PI and iMFV/MBP,allowing for better post-thrombectomy management in ACIS patients.
文摘Traditional Chinese medicine(TCM)is gaining popularity in the treatment of psychiatric disorders[1,2]that can be described and treated from either an Eastern or a Western perspective.In Eastern medicine,the disorders are described according to fi ve diagnostic methods that
基金This work was supported by the African Development Bank(AFDB)capacity building initiatives through Nelson Mandela African Institution of Science and Technology.
文摘Aquaculture provides significant contributions to household food security,as the capture of wild fish from lakes,dams,and oceans do not meet the current demand for animal protein in Tanzania.Sustainable aquaculture requires well-established regulatory systems and extension services for good pond management practices and maintaining fish health by fish farmers.Fish farming is practiced widely in Tanzania,from small-to large-scale ponds and these farming systems are moving from extensive normal operations(low input demand)to intensive farming(high input demand).However,the industry is largely still operating at a subsistence level with low production.Bacterial infections have been occurring in these fish farms and will continue to be an issue of concern into the future.This review highlights the current challenges,successes,and prospects towards a sustainable aquaculture industry in Tanzania,including:limited extension services mirroring the limited knowledge by farmers regarding pond management practices;the inadequacy of funds to carry out fish disease research or implement a surveillance system;little expertise in fish disease diagnosis and treatment;and poor management options.To minimize disease outbreaks and optimize production in the future,we suggest a strengthening of extension services,augmented with on-farm knowledge transfer.Emphasis should be on pond management practices and fish disease management;the creation of a well-functioning fish disease surveillance system;and strengthening collaborative research on aquaculture between the government research institutions and academia.Establishing small cooperative fish farmer groups within the Aquaculture Association of Tanzania(AAT)for easy access to information is also recommended.
基金the Science Challenge Project(No.TZ2016005)the NationalNatural Science Foundation of China(Nos.11827807,61905007,11520101003,and 11861121001)the Strategic Priority Research Programof the Chinese Academy of Sciences(No.XDB16010200)。
文摘We propose a spatial diffraction diagnostic method via inserting a millimeter-gap double slit into the collimated terahertz beam to monitor the minute variation of the terahertz beam in strong-field terahertz sources,which is difficult to be resolved in conventional terahertz imaging systems.To verify the method,we intentionally fabricate tiny variations of the terahertz beam through tuning the iris for the infrared pumping beam before the tilted-pulse-front pumping setups.The phenomena can be well explained by the theory based on the tilted-pulse-front technique and terahertz diffraction.
文摘In this study,flowback-assisted minifrac tests were conducted in low-permeability shale and salt formations to measure the in situ stress.An injection/flowback testing protocol was implemented in each test to achieve accuracy and efficiency.Accurate and efficient injection/flowback testing is very important,given the impermeable nature of these formations and the need to complete each test as quickly as possible.Each flowback cycle yields a distinct and repeatable fracture closure signature,simplifying the interpretation of the fracture closure pressure.The objective of this paper is to share our field experience and to present a numerical analysis of the flowback test pressure responses,fracture closure behaviors,and fracture closure diagnostic methods.Examples from open-hole and casedhole minifrac tests are used to demonstrate site operation procedures.Then,two numerical models are presented for simulating the fracture closure behavior during a flowback test.Field evidence is provided to demonstrate that the fracture closure pressures from the flowback tests are identical to those from tests without flowback.The fracture closure diagnostic methods for flowback tests are discussed,and it is found that the G-function diagnostic method yields a distinct fracture closure signal during the flowback tests.This study is intended to provide additional insights regarding flowback tests by sharing our successes,experience,and knowledge,thereby benefiting the industry.
