The present study was undertaken to elucidate how the plastic properties of model membranes from native lipids of different tissues of rats change in the course of Macrovipera lebetina obtuse (MLO), Montivipera raddei...The present study was undertaken to elucidate how the plastic properties of model membranes from native lipids of different tissues of rats change in the course of Macrovipera lebetina obtuse (MLO), Montivipera raddei (MR) and Naja kaouthia (NK) venoms processing. The presence of viper venom in organism lead to increasing of the electrical resistance of BLMs from liver and muscle lipids approximately on a sequence, while the BLMs from brain lipids has not shown a noticeable differences of plastic properties compare the control. The same concentration of cobra venom leads to decreasing of electrical resistance of BLMs from 1011 Ohm till 108 Ohm. The low concentration of venom leads to appearance of channel activity. Especially it is noticeable in liver lipids in media of bivalen ions.展开更多
Electroporation (EP) is one of the important techniques for the introduction of genes and drugs into cells with intense pulsed electric field to induce nanometer-sized electropores on cell membranes.Recently,micro/nan...Electroporation (EP) is one of the important techniques for the introduction of genes and drugs into cells with intense pulsed electric field to induce nanometer-sized electropores on cell membranes.Recently,micro/nano technology has been applied to many novel micro EP devices which can not only significantly increase uptake of biomolecules,DNA transfection and cell viability,but also enable large-scale single-cell EP.However,most EP theories developed in the past three decades can not precisely predict the experimental results of EP of biological cells.With the advanced micro EP chips for large-scale single-cell EP experiments,more precise EP theoretical models can be developed to describe the complicated multiscale dynamic behavior of EP.展开更多
文摘The present study was undertaken to elucidate how the plastic properties of model membranes from native lipids of different tissues of rats change in the course of Macrovipera lebetina obtuse (MLO), Montivipera raddei (MR) and Naja kaouthia (NK) venoms processing. The presence of viper venom in organism lead to increasing of the electrical resistance of BLMs from liver and muscle lipids approximately on a sequence, while the BLMs from brain lipids has not shown a noticeable differences of plastic properties compare the control. The same concentration of cobra venom leads to decreasing of electrical resistance of BLMs from 1011 Ohm till 108 Ohm. The low concentration of venom leads to appearance of channel activity. Especially it is noticeable in liver lipids in media of bivalen ions.
基金supported by the Hong Kong RGC (Grant No. 615907)the King Abdullah University of Science and Technology (Grant No. SA-C0040/UK-C0016)
文摘Electroporation (EP) is one of the important techniques for the introduction of genes and drugs into cells with intense pulsed electric field to induce nanometer-sized electropores on cell membranes.Recently,micro/nano technology has been applied to many novel micro EP devices which can not only significantly increase uptake of biomolecules,DNA transfection and cell viability,but also enable large-scale single-cell EP.However,most EP theories developed in the past three decades can not precisely predict the experimental results of EP of biological cells.With the advanced micro EP chips for large-scale single-cell EP experiments,more precise EP theoretical models can be developed to describe the complicated multiscale dynamic behavior of EP.
