Objective:Ovarian cancer is one of the leading causes of death in women,because of late diagnosis,recurrence and resistance to chemotherapy.Therefore,it's necessary to design a novel sensitive and specific maker t...Objective:Ovarian cancer is one of the leading causes of death in women,because of late diagnosis,recurrence and resistance to chemotherapy.Therefore,it's necessary to design a novel sensitive and specific maker to diagnose and treat patients of ovarian cancer.However,this study intends to explore the expression and prognostic value of ENO1 in ovarian cancer.Method:ENO1 data were collected from Oncomine database,and then were performed subgroup analysis and systematic analysis.Meanwhile,the prognostic significance of ENO1 was analyzed by an online survival analysis tool"Kaplan-Meier Plotter"(KM plotter)database.Results:In Oncomine database,a total of 14 studies were involved ENO1 in ovarian cancer tissues and normal tissues,including a total of 1001 samples.Comparing with normal tissues,ENO1 expression is higher(P<0.05).Moreover,the overall survival of ENO1 high expression in ovarian cancer is closely related with high survival(P<0.05).Further study indicated that ENO1 expression showed negative relation to the prognosis of serous ovarian cancer patients while ENO1 expression showed no effects on the prognosis of endometrioid ovarian cancer patients.Conclusion:Through exploration of tumor gene information in Oncomine database,ENO1 was found highly expressed in ovarian cancer and related to the prognosis of ovarian cancer,which might be a new marker for ovarian cancer prognosis and alternative drug therapy.展开更多
Metabolic reprogramming is a hallmark of cancer,including lung cancer.However,the exact underlying mechanism and therapeutic potential are largely unknown.Here we report that protein arginine methyltransferase 6(PRMT6...Metabolic reprogramming is a hallmark of cancer,including lung cancer.However,the exact underlying mechanism and therapeutic potential are largely unknown.Here we report that protein arginine methyltransferase 6(PRMT6)is highly expressed in lung cancer and is required for cell metabolism,tumorigenicity,and cisplatin response of lung cancer.PRMT6 regulated the oxidative pentose phosphate pathway(PPP)flux and glycolysis pathway in human lung cancer by increasing the activity of 6-phosphogluconate dehydrogenase(6PGD)and a-enolase(ENO1).Furthermore,PRMT6 methylated R324 of 6PGD to enhancing its activity;while methylation at R9 and R372 of ENO1 promotes formation of active ENO1 dimers and 2-phosphoglycerate(2-PG)binding to ENO1,respectively.Lastly,targeting PRMT6 blocked the oxidative PPP flux,glycolysis pathway,and tumor growth,as well as enhanced the antitumor effects of cisplatin in lung cancer.Together,this study demonstrates that PRMT6 acts as a posttranslational modification(PTM)regulator of glucose metabolism,which leads to the pathogenesis of lung cancer.It was proven that the PRMT6-6PGD/ENO1 regulatory axis is an important determinant of carcinogenesis and may become a promising cancer therapeutic strategy.展开更多
文摘Objective:Ovarian cancer is one of the leading causes of death in women,because of late diagnosis,recurrence and resistance to chemotherapy.Therefore,it's necessary to design a novel sensitive and specific maker to diagnose and treat patients of ovarian cancer.However,this study intends to explore the expression and prognostic value of ENO1 in ovarian cancer.Method:ENO1 data were collected from Oncomine database,and then were performed subgroup analysis and systematic analysis.Meanwhile,the prognostic significance of ENO1 was analyzed by an online survival analysis tool"Kaplan-Meier Plotter"(KM plotter)database.Results:In Oncomine database,a total of 14 studies were involved ENO1 in ovarian cancer tissues and normal tissues,including a total of 1001 samples.Comparing with normal tissues,ENO1 expression is higher(P<0.05).Moreover,the overall survival of ENO1 high expression in ovarian cancer is closely related with high survival(P<0.05).Further study indicated that ENO1 expression showed negative relation to the prognosis of serous ovarian cancer patients while ENO1 expression showed no effects on the prognosis of endometrioid ovarian cancer patients.Conclusion:Through exploration of tumor gene information in Oncomine database,ENO1 was found highly expressed in ovarian cancer and related to the prognosis of ovarian cancer,which might be a new marker for ovarian cancer prognosis and alternative drug therapy.
基金supported by grants from the Natural Science Foundation of Tianjin(21JCZDJC00060,China)the National Nature Science Foundation of China(81973356,91957120,81902826,and 81672781)+4 种基金the Fundamental Research Funds for the Central Universities of Nankai University(3206054,91923101,63213082 and 92122017,China)the State Key Laboratory of Drug Research(SIMM2105KF-08,China)the National Key R&D Program of China(No.2018YFC2002000)the Innovative S&T Projects for Young Researchers of Tianjin Academy of Agricultural Science(grant No.201918,China)the Natural Science Foundation of Tianjin(19JCYBJC29600 and 21JCYBJC00180,China)。
文摘Metabolic reprogramming is a hallmark of cancer,including lung cancer.However,the exact underlying mechanism and therapeutic potential are largely unknown.Here we report that protein arginine methyltransferase 6(PRMT6)is highly expressed in lung cancer and is required for cell metabolism,tumorigenicity,and cisplatin response of lung cancer.PRMT6 regulated the oxidative pentose phosphate pathway(PPP)flux and glycolysis pathway in human lung cancer by increasing the activity of 6-phosphogluconate dehydrogenase(6PGD)and a-enolase(ENO1).Furthermore,PRMT6 methylated R324 of 6PGD to enhancing its activity;while methylation at R9 and R372 of ENO1 promotes formation of active ENO1 dimers and 2-phosphoglycerate(2-PG)binding to ENO1,respectively.Lastly,targeting PRMT6 blocked the oxidative PPP flux,glycolysis pathway,and tumor growth,as well as enhanced the antitumor effects of cisplatin in lung cancer.Together,this study demonstrates that PRMT6 acts as a posttranslational modification(PTM)regulator of glucose metabolism,which leads to the pathogenesis of lung cancer.It was proven that the PRMT6-6PGD/ENO1 regulatory axis is an important determinant of carcinogenesis and may become a promising cancer therapeutic strategy.
