[Objectives] To investigate the role of Eriocalyxin B (EriB) in promoting colon cancer cell apoptosis through ERK1/2 pathway in vitro, and to provide a natural candidate drug for colon cancer treatment. [Methods] Colo...[Objectives] To investigate the role of Eriocalyxin B (EriB) in promoting colon cancer cell apoptosis through ERK1/2 pathway in vitro, and to provide a natural candidate drug for colon cancer treatment. [Methods] Colon cancer cells treated with different concentrations of EriB were detected by CCK-8 assay;cell scratch assay and crystal violet staining were used to detect the invasion and migration of colon cancer cell;cell apoptosis was detected by Annexin V/PI double staining, and cell cycle was detected by PI staining;Western Blotting was used to detect epithelial-mesenchymal transition and apoptosis-related proteins in colon cancer cells treated with EriB. [Results] After EriB treatment, the proliferation, migration and apoptosis of colon cancer cells were significantly inhibited, and the ratio of P-ERK1/2 to ERK was significantly decreased. [Conclusions] EriB can effectively inhibit the proliferation of colon cancer cells and promote the apoptosis of colon cancer cells through ERK1/2 pathway.展开更多
Eriocalyxin B,an ent-Kaurene diterpenoid extracted from a traditional Chinese herb Isodon eriocalyx,has been shown to possess multifunctional activities such as anti-cancer and anti-inflammatory.However,the function a...Eriocalyxin B,an ent-Kaurene diterpenoid extracted from a traditional Chinese herb Isodon eriocalyx,has been shown to possess multifunctional activities such as anti-cancer and anti-inflammatory.However,the function and mechanism of the compound in adipocyte differentiation is still unknown.Here we reported that eriocalyxin B blunted adipogenesis remarkably by inhibiting the accumulation of lipid droplets,triglycerides and the expressions of adipogenesis-related factors,including C/EBPβ,C/EBPα,PPARγ,and FABP4.Moreover,we showed that the inhibition might be the consequence of cell cycle being arrested at the G2/M phase during the mitotic clonal expansion of adipocyte differentiation,most likely by suppress-ing mRNAs and proteins of CDK1,CDK2,Cyclin A and Cyclin B1.Overall,we conclude that eriocalyxin B is capable of inhibiting adipocyte differentiation at the early stage through downregulating the proteins involved in cell cycle progression.展开更多
OBJECTIVE Eriocalyxin B(EriB)is a natural diterpenoid purified fromIsodoneriocalyxvar.laxiflora,traditional Chinese herbal medicines,possesses strong antileukemic activity with low toxicity.In murine t(8;21)leukemia m...OBJECTIVE Eriocalyxin B(EriB)is a natural diterpenoid purified fromIsodoneriocalyxvar.laxiflora,traditional Chinese herbal medicines,possesses strong antileukemic activity with low toxicity.In murine t(8;21)leukemia models,EriB remarkably prolong the survival time and decreased the xenograft tumor size by targeting AML1-ETO oncoprotein.In angiogenesis research by the highly vascularized chorioallantoic membrane(CAM)of the chicken embryo further confirm its antiangiogenic activity.Microarray offers a high efficient approach to study the gene expression profile treated by EriB,so as to provide systematic information about potential mechanisms of EriB curing AML.METHODS The t(8;21)AML cell line Kasumi-1 is most sensitive to EriB.Cells are treated with Eri-B(0.5μmol·L-1)and collected in 2,6,12 and 24h,respectively.Using human cDNA microarray,we investigate the changes of differential gene expression of Kasumi-1cells by time before and after Eri-B treatment.Meanwhile,the mRNA expression of TRAF2,DEDD2,BAG3,SAT1,IQGAP1,C-myc and GRB2 is detected by semi-quantitative RT-PCR and real-time quantitative PCR.RESULTS The genes regulated significantly are correlated with cell proliferation,apoptosis,cell circle,regulation of transcription,response to stimulies and metabolism.Regulation of TNFR mediated apoptotic signaling by EriB plays a key role to induce apoptosis and cell circle,involved NF-κB,Ras-MAPK,cAMP/PKA,PI3K/Akt,Cyt c/caspase and p53-Rb signal pathways.After detected by SqRT-PCR and real-time quantitative PCR,the mRNA expressions of BAG3,DEDD2,SAT1 and C-myc are significantly changed.CONCLUSION These findings describes the probable mechanisms involved and the value of EriB as a promising candidate targeting apoptosis cascade and cell circle in treatment of AML.展开更多
This review provides an overview of diterpene synthases which were initially identified via genetic and/or biochemical means,traversing all organisms researched to date.
Three new diterpenoids, eriocalyxins C-E, were isolated from Isodon eriocalyx. Their structures were elucidated as 66-hydroxy- 15β-acetoxy-3α, 20-epoxy-16β, 17-epoxy-ent-kaur-1,7-dione, 1α, 7β-dihydroxy-6β, 15β...Three new diterpenoids, eriocalyxins C-E, were isolated from Isodon eriocalyx. Their structures were elucidated as 66-hydroxy- 15β-acetoxy-3α, 20-epoxy-16β, 17-epoxy-ent-kaur-1,7-dione, 1α, 7β-dihydroxy-6β, 15β-diacetoxy-7, 20-epoxy-ent-kaur-16-ene, and 15β-acetoxy-1, 6-dioxo-6, 7-seco-ent-kaur-2, 16-dien-7, 20-olide by means of spectroscopic methods, including two-dimensional NMR techniques.展开更多
基金Supported by Natural Science Research Project of Colleges and Universities in Anhui Province(KJ2019A1110,KJ2020A0863)Innovation Team Research Fund Project of Anhui Medical College(WJH202007t,WJH202008t)2021 Anhui Provincial Production-Education Integration Training Base Project for Quality Engineering(2021cjrh020).
文摘[Objectives] To investigate the role of Eriocalyxin B (EriB) in promoting colon cancer cell apoptosis through ERK1/2 pathway in vitro, and to provide a natural candidate drug for colon cancer treatment. [Methods] Colon cancer cells treated with different concentrations of EriB were detected by CCK-8 assay;cell scratch assay and crystal violet staining were used to detect the invasion and migration of colon cancer cell;cell apoptosis was detected by Annexin V/PI double staining, and cell cycle was detected by PI staining;Western Blotting was used to detect epithelial-mesenchymal transition and apoptosis-related proteins in colon cancer cells treated with EriB. [Results] After EriB treatment, the proliferation, migration and apoptosis of colon cancer cells were significantly inhibited, and the ratio of P-ERK1/2 to ERK was significantly decreased. [Conclusions] EriB can effectively inhibit the proliferation of colon cancer cells and promote the apoptosis of colon cancer cells through ERK1/2 pathway.
基金supported by National Key R&D Program of China,(2017YFC1700906)Yunnan Provincial Science and Technology Department,China(2017FA044 and 2013HA023).
文摘Eriocalyxin B,an ent-Kaurene diterpenoid extracted from a traditional Chinese herb Isodon eriocalyx,has been shown to possess multifunctional activities such as anti-cancer and anti-inflammatory.However,the function and mechanism of the compound in adipocyte differentiation is still unknown.Here we reported that eriocalyxin B blunted adipogenesis remarkably by inhibiting the accumulation of lipid droplets,triglycerides and the expressions of adipogenesis-related factors,including C/EBPβ,C/EBPα,PPARγ,and FABP4.Moreover,we showed that the inhibition might be the consequence of cell cycle being arrested at the G2/M phase during the mitotic clonal expansion of adipocyte differentiation,most likely by suppress-ing mRNAs and proteins of CDK1,CDK2,Cyclin A and Cyclin B1.Overall,we conclude that eriocalyxin B is capable of inhibiting adipocyte differentiation at the early stage through downregulating the proteins involved in cell cycle progression.
基金The project supported by National Natural Science Foundation of China(30772637)Yunnan Provincial Science and Technology Department(2014IA033)
文摘OBJECTIVE Eriocalyxin B(EriB)is a natural diterpenoid purified fromIsodoneriocalyxvar.laxiflora,traditional Chinese herbal medicines,possesses strong antileukemic activity with low toxicity.In murine t(8;21)leukemia models,EriB remarkably prolong the survival time and decreased the xenograft tumor size by targeting AML1-ETO oncoprotein.In angiogenesis research by the highly vascularized chorioallantoic membrane(CAM)of the chicken embryo further confirm its antiangiogenic activity.Microarray offers a high efficient approach to study the gene expression profile treated by EriB,so as to provide systematic information about potential mechanisms of EriB curing AML.METHODS The t(8;21)AML cell line Kasumi-1 is most sensitive to EriB.Cells are treated with Eri-B(0.5μmol·L-1)and collected in 2,6,12 and 24h,respectively.Using human cDNA microarray,we investigate the changes of differential gene expression of Kasumi-1cells by time before and after Eri-B treatment.Meanwhile,the mRNA expression of TRAF2,DEDD2,BAG3,SAT1,IQGAP1,C-myc and GRB2 is detected by semi-quantitative RT-PCR and real-time quantitative PCR.RESULTS The genes regulated significantly are correlated with cell proliferation,apoptosis,cell circle,regulation of transcription,response to stimulies and metabolism.Regulation of TNFR mediated apoptotic signaling by EriB plays a key role to induce apoptosis and cell circle,involved NF-κB,Ras-MAPK,cAMP/PKA,PI3K/Akt,Cyt c/caspase and p53-Rb signal pathways.After detected by SqRT-PCR and real-time quantitative PCR,the mRNA expressions of BAG3,DEDD2,SAT1 and C-myc are significantly changed.CONCLUSION These findings describes the probable mechanisms involved and the value of EriB as a promising candidate targeting apoptosis cascade and cell circle in treatment of AML.
基金This study was supported by grants from the National Key Laboratory of Plant Molecular Genetics,and from the State Key Laboratory of Phytochemistry and Plant Resources in West China.
文摘This review provides an overview of diterpene synthases which were initially identified via genetic and/or biochemical means,traversing all organisms researched to date.
文摘Three new diterpenoids, eriocalyxins C-E, were isolated from Isodon eriocalyx. Their structures were elucidated as 66-hydroxy- 15β-acetoxy-3α, 20-epoxy-16β, 17-epoxy-ent-kaur-1,7-dione, 1α, 7β-dihydroxy-6β, 15β-diacetoxy-7, 20-epoxy-ent-kaur-16-ene, and 15β-acetoxy-1, 6-dioxo-6, 7-seco-ent-kaur-2, 16-dien-7, 20-olide by means of spectroscopic methods, including two-dimensional NMR techniques.
