Background The feed intake of sows during lactation is often lower than their needs.High-fiber feed is usually used during gestation to increase the voluntary feed intake of sows during lactation.However,the mechanism...Background The feed intake of sows during lactation is often lower than their needs.High-fiber feed is usually used during gestation to increase the voluntary feed intake of sows during lactation.However,the mechanism underlying the effect of bulky diets on the appetites of sows during lactation have not been fully clarified.The current study was conducted to determine whether a high-fiber diet during gestation improves lactational feed intake(LFI)of sows by modulating gut microbiota.Methods We selected an appropriate high-fiber diet during gestation and utilized the fecal microbial transplanta-tion(FMT)method to conduct research on the role of the gut microbiota in feed intake regulation of sows during lactation,as follows:high-fiber(HF)diet during gestation(n=23),low-fiber(LF)diet during gestation(n=23),and low-fiber diet+HF-FMT(LFM)during gestation(n=23).Results Compared with the LF,sows in the HF and LFM groups had a higher LFI,while the sows also had higher peptide tyrosine tyrosine and glucagon-like peptide 1 on d 110 of gestation(G110 d).The litter weight gain of piglets during lactation and weaning weight of piglets from LFM group were higher than LF group.Sows given a HF diet had lower Proteobacteria,especially Escherichia-Shigella,on G110 d and higher Lactobacillus,especially Lactobacil-lus_mucosae_LM1 and Lactobacillus_amylovorus,on d 7 of lactation(L7 d).The abundance of Escherichia-Shigella was reduced by HF-FMT in numerically compared with the LF.In addition,HF and HF-FMT both decreased the perinatal concentrations of proinflammatory factors,such as endotoxin(ET),lipocalin-2(LCN-2),tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β).The concentration of ET and LCN-2 and the abundance of Proteobacteria and Escheri-chia-Shigella were negatively correlated with the LFI of sows.Conclusion The high abundance of Proteobacteria,especially Escherichia-Shigella of LF sows in late gestation,led to increased endotoxin levels,which result in inflammatory responses and adverse effects on the LFI of sows.Add-ing HF during gestation reverses this process by increasing the abundance of Lactobacillus,especially Lactobacil-lus_mucosae_LM1 and Lactobacillus_amylovorus.展开更多
目的了解细菌感染性腹泻的临床及病原学特点.方法回顾性分析2012-2014年新疆医科大学第一附属医院216例感染性腹泻患者的临床及病原学特点.计数资料行χ2检验.结果3年间因感染性腹泻就诊的3215例患者中,216例粪细菌培养阳性,包括致病性...目的了解细菌感染性腹泻的临床及病原学特点.方法回顾性分析2012-2014年新疆医科大学第一附属医院216例感染性腹泻患者的临床及病原学特点.计数资料行χ2检验.结果3年间因感染性腹泻就诊的3215例患者中,216例粪细菌培养阳性,包括致病性大肠埃希菌(Escherichia coli,E.coli)感染112例(51.8%),志贺菌属感染64例(29.6%),副溶血弧菌感染23例(10.6%),沙门菌属感染17例(7.8%);腹泻就诊者在每年5-10 m o多见;致病性E.coli感染临床表现为腹痛、腹泻、恶心、呕吐,可伴有脱水,志贺菌属感染临床表现为发热、腹痛、腹泻、里急后重感.结论感染性腹泻患者粪细菌培养阳性率不高,仍以E.coli、志贺菌属为主,不同细菌感染腹泻患者临床表现有差异.展开更多
基金supported by National Science Foundation of Sichuan Province[2023NSFSC0010]National Natural Science Foundation of China[32230102]+1 种基金the Major Scientific and Technological Special Project of Sichuan Province[No.2021ZDZX0009]National Modern Agricultural Industry Technology System Sichuan Pig innovation team[scsztd-2023-08-03].
文摘Background The feed intake of sows during lactation is often lower than their needs.High-fiber feed is usually used during gestation to increase the voluntary feed intake of sows during lactation.However,the mechanism underlying the effect of bulky diets on the appetites of sows during lactation have not been fully clarified.The current study was conducted to determine whether a high-fiber diet during gestation improves lactational feed intake(LFI)of sows by modulating gut microbiota.Methods We selected an appropriate high-fiber diet during gestation and utilized the fecal microbial transplanta-tion(FMT)method to conduct research on the role of the gut microbiota in feed intake regulation of sows during lactation,as follows:high-fiber(HF)diet during gestation(n=23),low-fiber(LF)diet during gestation(n=23),and low-fiber diet+HF-FMT(LFM)during gestation(n=23).Results Compared with the LF,sows in the HF and LFM groups had a higher LFI,while the sows also had higher peptide tyrosine tyrosine and glucagon-like peptide 1 on d 110 of gestation(G110 d).The litter weight gain of piglets during lactation and weaning weight of piglets from LFM group were higher than LF group.Sows given a HF diet had lower Proteobacteria,especially Escherichia-Shigella,on G110 d and higher Lactobacillus,especially Lactobacil-lus_mucosae_LM1 and Lactobacillus_amylovorus,on d 7 of lactation(L7 d).The abundance of Escherichia-Shigella was reduced by HF-FMT in numerically compared with the LF.In addition,HF and HF-FMT both decreased the perinatal concentrations of proinflammatory factors,such as endotoxin(ET),lipocalin-2(LCN-2),tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β).The concentration of ET and LCN-2 and the abundance of Proteobacteria and Escheri-chia-Shigella were negatively correlated with the LFI of sows.Conclusion The high abundance of Proteobacteria,especially Escherichia-Shigella of LF sows in late gestation,led to increased endotoxin levels,which result in inflammatory responses and adverse effects on the LFI of sows.Add-ing HF during gestation reverses this process by increasing the abundance of Lactobacillus,especially Lactobacil-lus_mucosae_LM1 and Lactobacillus_amylovorus.
文摘目的了解细菌感染性腹泻的临床及病原学特点.方法回顾性分析2012-2014年新疆医科大学第一附属医院216例感染性腹泻患者的临床及病原学特点.计数资料行χ2检验.结果3年间因感染性腹泻就诊的3215例患者中,216例粪细菌培养阳性,包括致病性大肠埃希菌(Escherichia coli,E.coli)感染112例(51.8%),志贺菌属感染64例(29.6%),副溶血弧菌感染23例(10.6%),沙门菌属感染17例(7.8%);腹泻就诊者在每年5-10 m o多见;致病性E.coli感染临床表现为腹痛、腹泻、恶心、呕吐,可伴有脱水,志贺菌属感染临床表现为发热、腹痛、腹泻、里急后重感.结论感染性腹泻患者粪细菌培养阳性率不高,仍以E.coli、志贺菌属为主,不同细菌感染腹泻患者临床表现有差异.
