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Application of Patient Simulators Combined with Internet plus Scenario Simulation Teaching Models on Intravenous Infusion Nursing Education in China
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作者 Ying Wu Yun Chen +5 位作者 Liuyan Zhang Guohua Huang Jinai He Yutong Li Yuzhen Renqing Zhijuan Zhan 《Journal of Biosciences and Medicines》 2024年第1期64-71,共8页
Objective: To explore the effectiveness of applying patient simulators combined with Internet Plus scenario simulation teaching models on intravenous (IV) infusion nursing education, and to provide scientific evidence... Objective: To explore the effectiveness of applying patient simulators combined with Internet Plus scenario simulation teaching models on intravenous (IV) infusion nursing education, and to provide scientific evidence for the implementation of advanced teaching models in future nursing education. Methods: Enrolled 60 nurses who took the IV infusion therapy training program in our hospital from January 2022 to December 2023 for research. 30 nurses who were trained in traditional teaching models from January to December 2022 were selected as the control group, and 30 nurses who were trained with simulation-based teaching models with methods including simulated patients, internet, online meetings which can be replayed and scenario simulation, etc. from January to December 2023 were selected as the experimental group. Evaluated the learning outcomes based on the Competency Inventory for Nursing Students (CINS), Problem-Solving Inventory (PSI), comprehensive learning ability, scientific research ability, and proficiency in the theoretical knowledge and practical skills of IV infusion therapy. Nursing quality, the incidence of IV infusion therapy complications and nurse satisfaction with different teaching models were also measured. Results: The scientific research ability, PSI scores, CINS scores, and comprehensive learning ability of the experimental group were better than those of the control group (P 0.05), and their assessment results of practical skills, nursing quality of IV infusion therapy during training, and satisfaction with teaching models were all better than those of the control group with statistical significance (P < 0.05). The incidence of IV infusion therapy complications in the experimental group was lower than that in the control group, indicating statistical significance (P < 0.05). Conclusions: Teaching models based on patient simulators combined with Internet Plus scenario simulation enable nursing students to learn more directly and practice at any time and in any place, and can improve their proficiency in IV infusion theoretical knowledge and skills (e.g. PICC catheterization), core competencies, problem-solving ability, comprehensive learning ability, scientific research ability and the ability to deal with complicated cases. Also, it helps provide high-quality nursing education, improve the nursing quality of IV therapy, reduce the incidence of related complications, and ensure the safety of patients with IV therapy. 展开更多
关键词 Specialty of Intravenous Infusion Therapy Nursing Education Patient Simulators Internet Plus Scenario Simulation teaching model
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A Study of Innovative College English Teaching Model for Global Competence
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作者 TAO Wei 《Sino-US English Teaching》 2024年第4期171-176,共6页
Against the backdrop of globalization and deepening international cooperation,countries are putting forward higher demands on the global competence of talents.In this context,college English teaching,as an important p... Against the backdrop of globalization and deepening international cooperation,countries are putting forward higher demands on the global competence of talents.In this context,college English teaching,as an important part of the higher education system,faces the challenge of cultivating globally competent talents.This study analyzes the core components of global competence through literature review,analyzes the deficiencies in existing college English teaching,and aims to explore and construct a college English teaching model that is compatible with the global competence framework.This study explores how to integrate global competency development into college English teaching from three aspects:curriculum content,teaching methodology,and assessment system.We expect that the proposed teaching model can effectively improve students’English application skills,intercultural communication skills,and critical thinking skills,so as to better meet the demand for global competence in the context of globalization. 展开更多
关键词 global competence college English teaching model
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Three Subjects and Seven Duties Model:Empowering Informatization Teaching Ability of Pre-Service Teachers
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作者 Jingjuan Shu Dongqi Xu 《Journal of Contemporary Educational Research》 2024年第1期13-24,共12页
By constructing a model,this study seeks to provide suggestions for strengthening pre-service teachers’informatization teaching ability.It aims to answer the following research questions:(1)What do the pre-service te... By constructing a model,this study seeks to provide suggestions for strengthening pre-service teachers’informatization teaching ability.It aims to answer the following research questions:(1)What do the pre-service teachers know about informatization instruction?What is their level of informatization teaching ability?(2)What factors influence pre-service teachers’ability to deliver informatization instruction?How to overcome the obstacles?The inquiry focuses on the theoretical exploration of informatization teaching ability,questionnaire,and interview analysis on the current condition and improvement of pre-service teachers’informatization teaching ability.The data from the questionnaire and interview of the pre-service teachers were evaluated from three dimensions:awareness and attitude towards informatization teaching,knowledge of informatization teaching,and the ability to apply informatization teaching skills innovatively and comprehensively.The 3S-7D(Three Subjects and Seven Duties)model consisting of three subjects and the seven duties that go with them is established.The three subjects include pre-service teachers,teacher educators,and higher education schools.The seven duties involve pre-service teachers demonstrating learning motivation,cross-discipline thinking,and information and communications technology mastery;teacher educators actively constructing informatization teaching practice and formative assessment;and higher education schools providing adequate hardware and software equipment while offering cooperative opportunities with primary and junior schools through institutionalization. 展开更多
关键词 Pre-service teachers Informatization teaching ability 3S-7D model
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Practical effect of different teaching modes in teaching gastrointestinal surgery nursing
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作者 Xiao-Juan Rong Zhen Ning 《World Journal of Gastrointestinal Surgery》 SCIE 2024年第1期166-172,共7页
BACKGROUND With the continuous development and progress of medical technology,the position of surgical nursing in the field of clinical medicine is becoming in-creasingly prominent.As an important branch of the surgic... BACKGROUND With the continuous development and progress of medical technology,the position of surgical nursing in the field of clinical medicine is becoming in-creasingly prominent.As an important branch of the surgical field,the nursing requirements and difficulty of gastrointestinal surgery are also increasing.In order to improve the teaching quality of nursing care in gastrointestinal surgery,many educators and researchers are actively exploring new teaching methods.Among them,the teaching method case-based learning(CBL),scene-simulated learning(SSL),task-based learning(TBL),combining self-evaluation and training mode is considered as an effective method.This method aims to help students to better master knowledge and skills and improve their comprehensive quality by cultivating their self-evaluation ability.AIM To explore the practical effect of CBL-SSL-TBL combined with training mode and student self-assessment in nursing teaching of gastrointestinal surgery.METHODS Seventy-one nursing interns in our hospital from December 2020 to December 2021 were selected.According to different teaching modes,they were divided into observation group CBL-SSL-TBL combined with training mode combined with student self-assessment and control group(conventional teaching mode),of which 36 were in observation group and 35 were in control group.The results of operational skills,theoretical knowledge,nursing students'satisfaction,learning effectiveness questionnaire and teaching effect were compared between the two groups.RESULTS Compared between the two groups,the operational skills and theoretical knowledge scores of the observation group were higher than those of the control group,and the difference was statistically significant(P<0.05).Compared between the two groups,the total satisfaction ratio of the observation group was higher than that of the control group,the difference was statistically significant(P<0.05).Compared between the two groups,the observation group was lower than the control group in the questionnaire results of learning efficacy,and the difference was statistically significant(P<0.05).Compared between the two groups,the proportion of thinking ability,subjective initiative and understanding of theoretical knowledge in the observation group was higher than that in the control group,the difference was statistically significant(P<0.05).CONCLUSION The use of CBL-SSL-TBL combined with training mode and student self-assessment in gastrointestinal surgery nursing teaching can improve the operational skills of nursing interns,theoretical knowledge and satisfaction scores of nursing students,improve the results of learning efficiency questionnaire and teaching effect,which can be popularized in clinical teaching. 展开更多
关键词 Gastrointestinal surgery Nursing teaching teaching model Practical effect Learning ability
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Analysis of the History and Current Situation of the Development of General Practice Teaching Clinics in China
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作者 Xiaotong Huo Yajun Lian 《Open Journal of Preventive Medicine》 2024年第2期29-38,共10页
A summary of the exploration of the teaching mode of the general practice teaching clinic, a summary of the deficiencies of the teaching clinic and a summary of the significance of the establishment of the general pra... A summary of the exploration of the teaching mode of the general practice teaching clinic, a summary of the deficiencies of the teaching clinic and a summary of the significance of the establishment of the general practice teaching clinic are presented with a view to promoting the development of general practice and cultivating more excellent successors in general practice. 展开更多
关键词 General Practice teaching Clinic teaching models teaching Deficiencies in the Clinic Significance of the teaching Clinic
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Analysis of an event study using the Fama–French five‑factor model:teaching approaches including spreadsheets and the R programming language
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作者 Monica Martinez‑Blasco Vanessa Serrano +1 位作者 Francesc Prior Jordi Cuadros 《Financial Innovation》 2023年第1期2042-2075,共34页
The current financial education framework has an increasing need to introduce tools that facilitate the application of theoretical models to real-world data and contexts.However,only a limited number of free tools are... The current financial education framework has an increasing need to introduce tools that facilitate the application of theoretical models to real-world data and contexts.However,only a limited number of free tools are available for this purpose.Given this lack of tools,the present study provides two approaches to facilitate the implementa-tion of an event study.The first approach consists of a set of MS Excel files based on the Fama–French five-factor model,which allows the application of the event study methodology in a semi-automatic manner.The second approach is an open-source R-programmed tool through which results can be obtained in the context of an event study without the need for programming knowledge.This tool widens the calculus possibilities provided by the first approach and offers the option to apply not only the Fama–French five-factor model but also other models that are common in the finan-cial literature.It is a user-friendly tool that enables reproducibility of the analysis and ensures that the calculations are free of manipulation errors.Both approaches are freely available and ready-to-use. 展开更多
关键词 Event study Fama–French five-factor model Financial education teaching innovation SPREADSHEET R programming language
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Problem-based Learning Combining Seminar Teaching Method for the Practice of Mathematical Modeling Course's Teaching Reform for Computer Discipline
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作者 Siwei Zhou Zhao Li 《计算机教育》 2023年第12期55-62,共8页
Mathematical modeling course has been one of the fast development courses in China since 1992,which mainly trains students’innovation ability.However,the teaching of mathematical modeling course is quite difficult si... Mathematical modeling course has been one of the fast development courses in China since 1992,which mainly trains students’innovation ability.However,the teaching of mathematical modeling course is quite difficult since it requires students to have a strong mathematical foundation,good ability to design algorithms,and programming skills.Besides,limited class hours and lack of interest in learning are the other reasons.To address these problems,according to the outcome-based education,we adopt the problem-based learning combined with a seminar mode in teaching.We customize cases related to computer and software engineering,start from simple problems in daily life,step by step deepen the difficulty,and finally refer to the professional application in computer and software engineering.Also,we focus on ability training rather than mathematical theory or programming language learning.Initially,we prepare the problem,related mathematic theory,and core code for students.Furtherly,we train them how to find the problem,and how to search the related mathematic theory and software tools by references for modeling and analysis.Moreover,we solve the problem of limited class hours by constructing an online resource learning library.After a semester of practical teaching,it has been shown that the interest and learning effectiveness of students have been increased and our reform plan has achieved good results. 展开更多
关键词 Mathematical modeling Problem-based learning teaching reform Computer education
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A New Model of Software Engineering Education and Exploration of Professional Curriculum Teaching
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作者 Qingzhen Xu Mingzhi Mao Niansheng Cheng 《计算机教育》 2023年第12期150-157,共8页
This paper focuses on the problems,opportunities,and challenges faced by software engineering education in the new era.We have studied the core ideas of the new model and reform,the specific measures implemented,and t... This paper focuses on the problems,opportunities,and challenges faced by software engineering education in the new era.We have studied the core ideas of the new model and reform,the specific measures implemented,and the challenges and solutions faced.The new model and reform must focus on cultivating practical abilities,introducing interdisciplinary knowledge,and strengthening innovation awareness and entrepreneurial spirit.The process of reform and innovation is carried out from the aspects of teaching methods,teaching means,and course performance evaluation in the teaching practice of software engineering courses.We adopt a method of“question guiding,simple and easy to understand,flexible and diverse,and emphasizing practical results”,optimizing the curriculum design,providing diverse learning opportunities,and establishing a platform for the industry-university-research cooperation.Our teaching philosophy is to adhere to the viewpoint of innovative teaching ideas,optimizing teaching methods and teaching means,and comprehensively improving the teaching quality and level of software engineering education. 展开更多
关键词 Software engineering education New model Professional course teaching
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Practicability Evaluation and Improvement of Bloodletting Model Mold in Mongolian Medicine Teaching
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作者 Na ZHU Yongjun FENG 《Medicinal Plant》 CAS 2023年第4期99-102,共4页
According to the problems such as low level and depth of experimental teaching research of Mongolian medicine in higher vocational colleges, lack of applicable teaching model mold, lack of students practical operation... According to the problems such as low level and depth of experimental teaching research of Mongolian medicine in higher vocational colleges, lack of applicable teaching model mold, lack of students practical operation in time, and single teaching, the development direction and ideas of bloodletting model mold in Mongolian medicine in vocational Mongolian medicine teaching were proposed to provide valuable reference for the experimental teaching reform of traditional therapy of Mongolian medicine and the teaching reform and development ideas of Mongolian medicine and other traditional medicine. 展开更多
关键词 Specialty of Mongolian medicine Higher vocational education Experimental teaching of bloodletting in Mongolian medicine Bloodletting model mold of Mongolian medicine
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Influence of Online Learning Attitude in the Blended Teaching Model on the Final Grades
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作者 YUE Wen-ting WANG Jin-jun ZHAO Yu 《Journal of Literature and Art Studies》 2023年第5期383-388,共6页
The combination of online teaching and traditional offline teaching can maximize the advantages of both.Based on the blended teaching of English Reading course,39 students were selected as the research subjects to stu... The combination of online teaching and traditional offline teaching can maximize the advantages of both.Based on the blended teaching of English Reading course,39 students were selected as the research subjects to study the relationship between their online learning attitudes and their grades in the final examination.Judged from the number of times for each student to download teaching resources,the number of assignments submitted online,and the quality of the submitted assignments,each student’s attitude toward online learning was examined comprehensively,and a correlation analysis was conducted through SPSS Statistics 21.0 to explore the influence of online learning attitude on English reading performance.Through data collection and analysis of the online learning attitudes over a 16-week period,a significant positive correlation was found between the online learning attitudes and the English reading grades,indicating that the online learning attitude in the blended learning model plays a crucial role in improving the English reading skill,and students should maintain a positive attitude toward online teaching in blended learning. 展开更多
关键词 blended teaching model online learning attitude correlation analysis final grade of English Reading
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“Flipped Classroom” Teaching Model Research: A Case Study of “Innovation and Entrepreneurship Education and Practice (Theory)”
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作者 Xiushu Zhao 《Open Journal of Applied Sciences》 2023年第11期2007-2013,共7页
The flipped classroom model differs significantly from the traditional classroom teaching model and offers numerous advantages. This article draws inspiration from the flipped classroom concept, using the “Innovation... The flipped classroom model differs significantly from the traditional classroom teaching model and offers numerous advantages. This article draws inspiration from the flipped classroom concept, using the “Innovation and Entrepreneurship Education and Practice (Theory)” course as an example to introduce the innovation and characteristics of the flipped classroom model: it is problem-oriented, addressing the “pain points” in teaching, motivating students towards “active learning,” and integrating diverse teaching methods. In the conclusion, the article proposed suggestions on the application of this model, encouraging teachers to summarize their teaching experiences, enhance their knowledge base, and foster students’ awareness of innovation and entrepreneurship. 展开更多
关键词 Flipped Classroom teaching model Research Innovation and Entrepreneurship Education and Practice Case
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Reform of the Teaching Model of Korean Intensive Reading Courses in Vocational High Schools in the Era of All-Media
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作者 Ruoxuan Chen 《Journal of Contemporary Educational Research》 2023年第11期17-23,共7页
The use of multimedia technology in the teaching process is the current mainstream trend in teaching development.The arrival of the all-media era is not only a huge challenge to the traditional teaching model,but also... The use of multimedia technology in the teaching process is the current mainstream trend in teaching development.The arrival of the all-media era is not only a huge challenge to the traditional teaching model,but also a huge opportunity for the reform of the teaching model.Combining traditional education models with modern scientific and technological means,and learning from each other’s strengths can stimulate students’subjective initiative in learning.On the other hand,it is also an important way for teachers to improve their teaching abilities.This article analyzes the opportunities brought by the all-media era to the reform of education models based on the teaching status and teaching difficulties of Korean intensive reading courses in vocational high schools,and proposes a teaching model reform strategy for Korean intensive reading courses in vocational high schools in the all-media era. 展开更多
关键词 All-media era teaching model reform Vocational high school teaching Korean teaching
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Research on the Evaluation of VBSE Practical Training Teaching Effectiveness Based on CIPP Model at X University
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作者 Sisi Chen Lingzhi Liao 《Journal of Contemporary Educational Research》 2023年第12期100-104,共5页
Based on the CIPP(context,input,process,and product)model,this paper constructs an index system suitable for evaluating the teaching effect of VBSE(virtual business social environment)practical training from four aspe... Based on the CIPP(context,input,process,and product)model,this paper constructs an index system suitable for evaluating the teaching effect of VBSE(virtual business social environment)practical training from four aspects:context evaluation,input evaluation,process evaluation,and product evaluation.Taking the students participating in VBSE practical training in X university as the survey population,381 valid sample data were obtained through an online questionnaire survey,and the index weights were determined by factor analysis method.The score value of the VBSE practical training teaching effect was calculated based on the evaluation mean value of three indexes.The results showed that the context evaluation score was 1.56,the input evaluation score was 1.54,the process evaluation score was 1.51,and the product evaluation score was 1.48.Subsequently,this paper put forward some countermeasures from the aspects of optimizing course arrangement,improving hardware facilities,and enhancing team cooperation to provide a guideline for improving the effect of VBSE practical training. 展开更多
关键词 VBSE practical training teaching CIPP model teaching effect evaluation
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Practical Progress of SBAR Communication Model-Related Teaching in Clinical Teaching
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作者 Xu Liu Meili Ma +2 位作者 Chenfei Qu Chunlin Liu Yan Yin 《Journal of Clinical and Nursing Research》 2023年第1期7-14,共8页
This paper elaborates the core and essence of situation,background,assessment,recommendation(SBAR)communication model,clarify the application status of SBAR communication model in the clinical teaching of nursing stud... This paper elaborates the core and essence of situation,background,assessment,recommendation(SBAR)communication model,clarify the application status of SBAR communication model in the clinical teaching of nursing students,and describe its effectiveness and limitations,so as to provide reference for improving clinical nursing teaching ability. 展开更多
关键词 SBAR communication model Clinical teaching Nursing education PROGRESS
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Application of the Just-in-Time Teaching(JiTT)Model in Bilingual Courses of Chinese-Foreign Cooperative Education
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作者 Na Yao Qingwen Yuan Ping Yu 《Journal of Contemporary Educational Research》 2023年第12期86-91,共6页
The teaching abilities of bilingual teachers have emerged as one of the major bottlenecks hindering the further development of Chinese-foreign cooperative education projects.Implementing the JiTT(Just-in-Time Teaching... The teaching abilities of bilingual teachers have emerged as one of the major bottlenecks hindering the further development of Chinese-foreign cooperative education projects.Implementing the JiTT(Just-in-Time Teaching)model in bilingual courses of Chinese-foreign cooperative education can effectively integrate information technology with traditional classroom teaching.This integration enhances teaching quality and effectiveness,encourages university instructors to improve their diverse and collective capabilities,promotes digital development,and contributes to the enhancement of students’learning abilities and overall improvement. 展开更多
关键词 Just-in-Time teaching model Information technology BILINGUAL
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Discussion on the Impact of“Shift System”Teaching Model of Physical Education on Students’Mental Health
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作者 Lanmin Shang 《Journal of Contemporary Educational Research》 2023年第12期71-76,共6页
The“shift system”teaching model of physical education is an emerging education model that aims to improve students’independent choice and personalized development.However,there are also some challenges in the pract... The“shift system”teaching model of physical education is an emerging education model that aims to improve students’independent choice and personalized development.However,there are also some challenges in the practical application of this model.For example,there are mental health issues for some students including difficulty in adaptation,social interaction,high psychological pressure,etc.Based on this,this article analyzes the impact of the“shift system”teaching model of physical education on students’mental health and explores the optimization path of the physical education“shift system”teaching model in order to promote students’mental health and all-round development. 展开更多
关键词 Physical education “Shift system”teaching model STUDENTS Mental health IMPACT
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Analysis on Application of Pragmatic Translation Skill Structure Model in Translation Teaching
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作者 宋俐娟 《疯狂英语(理论版)》 2016年第3期155-156,共2页
As the core content of pragmatic translation teaching research,pragmatic translation skill is closely correlated with translation technique and operation field.Viewed from the current English teaching situation in col... As the core content of pragmatic translation teaching research,pragmatic translation skill is closely correlated with translation technique and operation field.Viewed from the current English teaching situation in colleges and universities,translation skill is not cultivated as an independent language competence.Moreover,its research is insufficient.On account of this,the author first gives an introduction to pragmatic translation skill and pragmatic translation skill structure model in this paper.On this basis,the author also analyzes the application of pragmatic translation skill structure model in translation teaching. 展开更多
关键词 pragmatic translation skill structure model translation teaching APPLICATION
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Exploring and Practicing a Teaching Model of Dialogue-scene Simulation in Medical English
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作者 周娟娟 《海外英语》 2018年第19期61-62,共2页
Practicing dialogue-scene simulation in medical English can integrate effectively learning resources, activate the class atmosphere, enrich contents of courses, train language skills and raise students' cooperated... Practicing dialogue-scene simulation in medical English can integrate effectively learning resources, activate the class atmosphere, enrich contents of courses, train language skills and raise students' cooperated consciousness.Furthermore,practicing dialogue-scene simulation can promote reform of teaching estimation-model, teaching quality and efficiency. In a word, practicing dialogue-scene simulation deserves further exploration and promotion. 展开更多
关键词 Medical English teaching model teaching reform
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A Study of Blended-teaching Model in Medical English
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作者 燕頔 《中国校外教育》 2018年第12期102-103,共2页
Based on the analysis of the current college Medical English teaching and the study of the blendedteaching model,the author practiced the model in the real teaching.In the process,the author verified the usefulness an... Based on the analysis of the current college Medical English teaching and the study of the blendedteaching model,the author practiced the model in the real teaching.In the process,the author verified the usefulness and effectiveness of the Blended-teaching Model,which can break the limitation of the classroom teaching.The students could contact with each other and also with the teacher conveniently whatever in-class and offclass in the learning process. 展开更多
关键词 Blended-teaching model MEDICAL ENGLISH
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Establishing an experimental model of photodynamic induced anterior ischemic optic neuropathy 被引量:3
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作者 Runsheng Wang Xiaodi Wang +6 位作者 Peilin Lue Jianwei Bai Jianzhou Wang Xiaoqin Lei Xiao- liang Zhou Hongfen Sun Aizhu Pan 《Neural Regeneration Research》 SCIE CAS CSCD 2006年第4期339-342,共4页
BACKGROUND: Scholars have supposed to establish animal models of optic neuropathy by pressing and partially amputating optic nerve, increasing intraocular pressure and injecting vasoconstrictor, etc., but the models a... BACKGROUND: Scholars have supposed to establish animal models of optic neuropathy by pressing and partially amputating optic nerve, increasing intraocular pressure and injecting vasoconstrictor, etc., but the models are greatly different from anterior ischemia optic neuropathy. Therefore, a more ideal method is needed to establish animal model of anterior ischemic optic neuropathy (AION).OBJECTIVE: To establish AION models in rats, observe the functional changes of fundus, fundus fluorescein angiography (FFA), optical coherence tomography (OCT), flash visual evoked potential (F-VEP), and histopathologically confirm its reliability.DESIGN: A randomized control trial.SETTINGS: Department of Ophthalmology, Xi’an Fourth Hospital; Xi’an Institute of Ocular Fundus Diseases.MATERIALS: The experiments were carried out in the research room of Xi’an Institute of Ocular Fundus Diseases from February 2005 to May 2006. Thirty healthy male SD rats of 4-5 weeks old, weighing 140-160 g, were provided by the animal experimental center of the Fourth Military Medical University of Chinese PLA [SCXK(Military)2002-005], and those without eye disease examined by slit lamp and direct ophthalmoscope after mydriasis were enrolled. The conditions for feeding mice without special pathogen were strictly followed. The rats were randomly divided into blank control group (n =5), laser group (n =5), hematoporphyrin derivative (HPD) group and AION group (n =15), each group was numbered randomly. For each rat, the right eye was taken as the experimental eye, and the left one as the control one.METHODS: In the AION group, the rats were injected with HPD (10 mg/kg) via caudal vein, and then the optic discs were exposed to krypton red (647 nm, 80 mV) for 120 s, and the rats were in avoidance of light for 2 weeks postoperatively. Rats in the laser group were only exposed to krypton red (647 nm, 80 mV) for 120 s, and in avoidance of light for 2 weeks postoperatively; Those in the HPD group were only injected with HPD (10 mg/kg) via caudal vein; Those in the blank control group were untouched. ① Visual electrophysiological test: The F-VEP was used to evaluate the function of visual nerve. ② FFA: After mydriasis and anesthesia as describe above, the fluorescein sodium parenteral solution (1 mL/kg) was injected via caudal vein and finished within about 3 s, the time of FFA was recorded from the beginning of injection, the video sight aimed at the optic disc and the surrounding area. ③ After mydriasis and anesthesia as describe above, the rats were examined with OCT. ④ Histological observation: After hematoxylin and eosin (HE) staining, the optic disc and surrounding blood vessels of retina were observed under light microscope at high power field. MAIN OUTCOME MEASURES: The results of fundus, FFA, visual electrophysiological test and OCT detection within 90 days after model establishment were observed. RESULTS: Of the 30 rats, 1 died after anesthesia in the laser group and 2 died in the AION group respectively, and finally 27 rats were involved in the analysis of results. ① Changes in fundus: In the AION group, there was edema in upper optic disc and unclear boundary at 1 day after establishment, edema still could be observed at 6 days, and upper optic disc atrophied and appeared as pale at 90 days. ② FFA results: In the AION group, early "low fluorescence", middle and late "high fluorescence" were observed in upper optic disc 1 day after model establishment, and there was "low fluorescence" at 6 days, and the low fluorescence could be observed all the time at 23 days. ③ Visual electrophysiological changes: In the AION group as compared with the control eyes, the experimental ones had prolonged F-VEP P100 latency [(71.65±8.81), (57.58±8.38) ms, t =3.148, P =0.012], and decreased wave amplitude [(4.77±1.90), (10.06±3.66) μV, t =4.082, P =0.003], and these changes lasted to 35 days after model establishment. ④ OCT results: In the AION group, the reflection surface of part nerve fiber layer was higher than the retina plane, the surface was rough and the thickness was increased at 6 days after model establishment. ⑤ Histopathological results: At 1 day after model establishment, part optic discs had highly edema, edema of nerve fibers, and loose tissue, also accompanied by the displacement of surrounding retina; At 23 days, the optic disc and surrounding nerve fiber layers became thinner, and the numbers of ganglion nuclei in the retina tissue sections were obviously decreased. These changes were not observed in the laser group, HPD group and blank control group. CONCLUSION: The changes of fundus, FFA, OCT, visual electrophysiology and histopathology confirmed that the krypton red laser irradiation (647 nm) at 2 hours after HPD was injected via caudal vein can establish more ideal animal models of AION. 展开更多
关键词 AION establishing an experimental model of photodynamic induced anterior ischemic optic neuropathy
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