Background: Escherichia coli are ubiquitous bacteria colonising both humans and animals. Extended spectrum β-lactamase-producing E. coli has been selected as a suitable indicator for the monitoring and surveillance o...Background: Escherichia coli are ubiquitous bacteria colonising both humans and animals. Extended spectrum β-lactamase-producing E. coli has been selected as a suitable indicator for the monitoring and surveillance of antimicrobial resistance. Death due to resistant bacteria is continuously rising in Cameroon, but the contribution of the aviary sector is not well studied. Therefore, this study aimed to investigate the resistance profile of extended spectrum beta-lactamases-producing Escherichia coli strains, isolated from faeces of broiler chickens in Yaoundé, capital city of Cameroon. Methods: A cross-sectional descriptive study was carried out from February to June 2020. Escherichia coli were isolated from samples of broilers in poultry farms in Yaoundé and submitted to the extended spectrum β-lactamase screening. The logistic regression was used to assess the statistical association of a significance threshold p-value of 0.05. Results: Out of 385 faecal samples collected in broiler farms, 114 Escherichia coli isolates were obtained out of which 30 (26.32%) were Extended Spectrum Beta-Lactamases-producing Escherichia coli. These isolates revealed high resistance to all antibiotic families. Poor storage conditions for feeds and the proximity to latrines, the troughs on the ground, the lack of foot bath and uniforms, the inadequate treatment of faeces, the poor usage of preventive antibiotics and the lack of water treatment have been identified as risk factors to faecal carriage of ESBL-producing Escherichia coli. Conclusion: This work reveals the emergence of Extended Spectrum Beta-Lactamases-producing Escherichia coli in poultry farms in Yaoundé and the failure in the biosecurity system. As such, the awareness of poultry breeders on the respect of biosecurity measures may be an effective tool to tackle antimicrobial resistance, specifically in livestock industries using a One Health approach.展开更多
In order to investigate the clinical and microbiological characteristics of patients with complicated intra-abdominal infections(cIAIs)in intensive care unit(ICU),the clinical data of 612 cIAIs patients from January 2...In order to investigate the clinical and microbiological characteristics of patients with complicated intra-abdominal infections(cIAIs)in intensive care unit(ICU),the clinical data of 612 cIAIs patients from January 2016 to December 2018 were retrospectively collected.Clinical characteristics,distribution of pathogens and drug resistance were statistically analyzed.It was found that patients with community-acquired intra-abdominal infections(CA-IAIs)made up a majority of cIAIs patients.The positive rate of abdominal drainage fluid culture was 55.56%.Gramnegative bacteria accounted for the majority,the most commonly isolated bacteria of which were Escherichia coli(20.96%),Klebsiella pneumoniae(10.20%)and Pseudomonas aeruginosa(5.57%).The most commonly isolated gram-positive bacteria were Enterococcus(16.88%)and Methicillinresistant staphylococcus aureus(MRSA,3.90%).Enterobacter isolates showed high resistance rate to most cephalosporins and low resistance rate to piperacillin/tazobactam and carbapenems.Extended spectrum beta-lactamase(ESBL)screen positive isolates from CA-IAIs patients showed an increasing trend in past three years.Enterococcus and MRSA showed high resistance rate to clindamycin,quinolone,erythromycin and tetracycline,while they showed high sensitivity rate to linezolid,tegacycline,teicoplanin and vancomycin.Our results indicate that isolated bacteria from abdominal drainage fluid show high resistance rates to commonly used antibiotics in ICU patients with cIAIs.The curative effects on diseases should be monitored continuously when antibiotics are used.Meanwhile,we should always keep eyes on drug-resistant bacteria,especially when the treatment efficacy is not good.展开更多
Escherichia coli is a prevalent causative pathogen of spontaneous bacterial peritonitis(SBP).In this retrospective study,we investigated the microbiological characteristics and antibiotic susceptibility of E.coli clin...Escherichia coli is a prevalent causative pathogen of spontaneous bacterial peritonitis(SBP).In this retrospective study,we investigated the microbiological characteristics and antibiotic susceptibility of E.coli clinical isolates obtained from liver cirrhosis patients suffering from nosocomial SBP.Our results showed that extended-spectrum b-lactamase(ESBL)-producing E.coli accounted for 47%of the cases,while 62%of the isolates were multi-drug resistant(MDR)pathogens.ESBL-producing and MDR isolates showed high incidences of resistance to third-generation cephalosporins,but they displayed susceptibility to carbapenems,b-lactamase inhibitors,and aminoglycosides.Importantly,liver cirrhosis patients with MDR E.coli SBP showed a significantly higher death rate than patients with non-MDR infections(P=0.021).The 30-day mortality of nosocomial SBP was independently correlated with female gender[odds ratio(OR)=5.200,95%confidence interval(CI)=1.194–22.642],liver failure(OR=9.609,95%CI=1.914–48.225),hepatocellular carcinoma(OR=8.176,95%CI=2.065–32.364),hepatic encephalopathy(OR=8.176,95%CI=2.065–32.364),model of end-stage liver disease score(OR=1.191,95%CI=1.053–1.346),white blood cell count(OR=0.847,95%CI=0.737–0.973),and ascites polymorphonuclear(OR=95.903,95%CI=3.410–2697.356).In conclusion,third-generation cephalosporins may be inappropriate for empiric treatment of nosocomial SBP caused by E.coli,due to the widespread presence of ESBLs and high incidence of MDR pathogens.展开更多
文摘Background: Escherichia coli are ubiquitous bacteria colonising both humans and animals. Extended spectrum β-lactamase-producing E. coli has been selected as a suitable indicator for the monitoring and surveillance of antimicrobial resistance. Death due to resistant bacteria is continuously rising in Cameroon, but the contribution of the aviary sector is not well studied. Therefore, this study aimed to investigate the resistance profile of extended spectrum beta-lactamases-producing Escherichia coli strains, isolated from faeces of broiler chickens in Yaoundé, capital city of Cameroon. Methods: A cross-sectional descriptive study was carried out from February to June 2020. Escherichia coli were isolated from samples of broilers in poultry farms in Yaoundé and submitted to the extended spectrum β-lactamase screening. The logistic regression was used to assess the statistical association of a significance threshold p-value of 0.05. Results: Out of 385 faecal samples collected in broiler farms, 114 Escherichia coli isolates were obtained out of which 30 (26.32%) were Extended Spectrum Beta-Lactamases-producing Escherichia coli. These isolates revealed high resistance to all antibiotic families. Poor storage conditions for feeds and the proximity to latrines, the troughs on the ground, the lack of foot bath and uniforms, the inadequate treatment of faeces, the poor usage of preventive antibiotics and the lack of water treatment have been identified as risk factors to faecal carriage of ESBL-producing Escherichia coli. Conclusion: This work reveals the emergence of Extended Spectrum Beta-Lactamases-producing Escherichia coli in poultry farms in Yaoundé and the failure in the biosecurity system. As such, the awareness of poultry breeders on the respect of biosecurity measures may be an effective tool to tackle antimicrobial resistance, specifically in livestock industries using a One Health approach.
基金gants from Research Foundation of Health and Family Planning Commission of Hubei Province(No.WJ2017M041)National Natural Science Foundation of China(No.81770283)Clinical Medical Research Center of Peritoneal Cancer of Wuhan,China(No.2015060911020462).
文摘In order to investigate the clinical and microbiological characteristics of patients with complicated intra-abdominal infections(cIAIs)in intensive care unit(ICU),the clinical data of 612 cIAIs patients from January 2016 to December 2018 were retrospectively collected.Clinical characteristics,distribution of pathogens and drug resistance were statistically analyzed.It was found that patients with community-acquired intra-abdominal infections(CA-IAIs)made up a majority of cIAIs patients.The positive rate of abdominal drainage fluid culture was 55.56%.Gramnegative bacteria accounted for the majority,the most commonly isolated bacteria of which were Escherichia coli(20.96%),Klebsiella pneumoniae(10.20%)and Pseudomonas aeruginosa(5.57%).The most commonly isolated gram-positive bacteria were Enterococcus(16.88%)and Methicillinresistant staphylococcus aureus(MRSA,3.90%).Enterobacter isolates showed high resistance rate to most cephalosporins and low resistance rate to piperacillin/tazobactam and carbapenems.Extended spectrum beta-lactamase(ESBL)screen positive isolates from CA-IAIs patients showed an increasing trend in past three years.Enterococcus and MRSA showed high resistance rate to clindamycin,quinolone,erythromycin and tetracycline,while they showed high sensitivity rate to linezolid,tegacycline,teicoplanin and vancomycin.Our results indicate that isolated bacteria from abdominal drainage fluid show high resistance rates to commonly used antibiotics in ICU patients with cIAIs.The curative effects on diseases should be monitored continuously when antibiotics are used.Meanwhile,we should always keep eyes on drug-resistant bacteria,especially when the treatment efficacy is not good.
基金the Special Fund of the Digestive Medical Coordinated Development Center of Beijing Municipal Administration(Grant number:XXZ0403).
文摘Escherichia coli is a prevalent causative pathogen of spontaneous bacterial peritonitis(SBP).In this retrospective study,we investigated the microbiological characteristics and antibiotic susceptibility of E.coli clinical isolates obtained from liver cirrhosis patients suffering from nosocomial SBP.Our results showed that extended-spectrum b-lactamase(ESBL)-producing E.coli accounted for 47%of the cases,while 62%of the isolates were multi-drug resistant(MDR)pathogens.ESBL-producing and MDR isolates showed high incidences of resistance to third-generation cephalosporins,but they displayed susceptibility to carbapenems,b-lactamase inhibitors,and aminoglycosides.Importantly,liver cirrhosis patients with MDR E.coli SBP showed a significantly higher death rate than patients with non-MDR infections(P=0.021).The 30-day mortality of nosocomial SBP was independently correlated with female gender[odds ratio(OR)=5.200,95%confidence interval(CI)=1.194–22.642],liver failure(OR=9.609,95%CI=1.914–48.225),hepatocellular carcinoma(OR=8.176,95%CI=2.065–32.364),hepatic encephalopathy(OR=8.176,95%CI=2.065–32.364),model of end-stage liver disease score(OR=1.191,95%CI=1.053–1.346),white blood cell count(OR=0.847,95%CI=0.737–0.973),and ascites polymorphonuclear(OR=95.903,95%CI=3.410–2697.356).In conclusion,third-generation cephalosporins may be inappropriate for empiric treatment of nosocomial SBP caused by E.coli,due to the widespread presence of ESBLs and high incidence of MDR pathogens.
