卵母细胞所特有的转录调节因子在卵子发生、卵泡形成和胚胎发育的早期等多个阶段中具有重要作用。文章综述了在雌性生殖系中特异性表达的转录调节因子缺失生殖系α因子基因(factor in the germline alpha,FIGLA)和新生儿卵巢同源盒基因(...卵母细胞所特有的转录调节因子在卵子发生、卵泡形成和胚胎发育的早期等多个阶段中具有重要作用。文章综述了在雌性生殖系中特异性表达的转录调节因子缺失生殖系α因子基因(factor in the germline alpha,FIGLA)和新生儿卵巢同源盒基因(newborn ovary homeobox Gene,NOBOX)在卵子发生过程中的研究进展,旨在为哺乳动物的生殖调控提供一定参考。展开更多
The factor in the germline alpha(figla), as a member of the basic helix-loop-helix family, has been reported to be involved in ovary development in mammals and teleosts. However, the regulatory mechanisms of figla in ...The factor in the germline alpha(figla), as a member of the basic helix-loop-helix family, has been reported to be involved in ovary development in mammals and teleosts. However, the regulatory mechanisms of figla in teleosts remain unclear. Here,figla in P. olivaceus(Pofigla) was characterized with encoding a 202 amino acid protein that contains a conserved basic region and helix-loop-helix(HLH) domain. Amino acids alignment and synteny analysis revealed that Pofigla was conserved with the orthologous gene sequences in other vertebrates. The results of qRT-PCR showed Pofigla was maternally inherited during embryonic development. For tissue distribution, Pofigla showed a sexually dimorphic gene expression in the gonad of different genders, with a higher expression in ovary than in testis. In situ hybridization(ISH) results demonstrated Pofigla was specifically expressed in germ cells including oocytes, spermatogonia and spermatocytes. By screening and analyzing two proximal regions(-2966/-2126 and-772/-444) with high promoter activity, we found SOX5, LEF1, FOXP1 and GATA1 may play important roles in the transcriptional regulation of Pofigla. Furthermore, we observed the co-localization between Figla and LEF1 in HEK 293T cells. And the significant up-regulation effect of the canonical Wnt signaling pathway on the expression of Pofigla was found in cultured ovarian cells. This study provided the first evidence that figla not only has an important function in ovary development, but also plays some potential roles in testis development and/or male germ cell differentiation during early testis development in P. olivaceus. The results provide valuable reference in exploring the regulatory network of figla in teleost.展开更多
利用real-time PCR和原位杂交技术分析了Figla(factor in the germline alpha)基因在湘西黄牛卵巢中的相对表达量与定位表达情况。结果显示:能冲出较多优质胚胎的湘西黄牛个体,卵巢组织中Figla基因mRNA水平的表达量要显著高于对照组(P&l...利用real-time PCR和原位杂交技术分析了Figla(factor in the germline alpha)基因在湘西黄牛卵巢中的相对表达量与定位表达情况。结果显示:能冲出较多优质胚胎的湘西黄牛个体,卵巢组织中Figla基因mRNA水平的表达量要显著高于对照组(P<0.05)。Figla基因mRNA在牛卵巢组织中的初级卵泡、次级卵泡和成熟的有腔卵泡都有表达。而且Figla基因在有腔卵泡的卵母细胞中的胞质中表达,但在细胞核中没有表达。结果说明:湘西黄牛超数排卵和冲胚效果与卵巢Figla基因表达水平之间存在一定的关系,Figla基因可能是卵泡和早期胚胎发育的双重关键基因。展开更多
文摘卵母细胞所特有的转录调节因子在卵子发生、卵泡形成和胚胎发育的早期等多个阶段中具有重要作用。文章综述了在雌性生殖系中特异性表达的转录调节因子缺失生殖系α因子基因(factor in the germline alpha,FIGLA)和新生儿卵巢同源盒基因(newborn ovary homeobox Gene,NOBOX)在卵子发生过程中的研究进展,旨在为哺乳动物的生殖调控提供一定参考。
基金supported by the National Key R&D Program of China (No. 2018YFD0901205)
文摘The factor in the germline alpha(figla), as a member of the basic helix-loop-helix family, has been reported to be involved in ovary development in mammals and teleosts. However, the regulatory mechanisms of figla in teleosts remain unclear. Here,figla in P. olivaceus(Pofigla) was characterized with encoding a 202 amino acid protein that contains a conserved basic region and helix-loop-helix(HLH) domain. Amino acids alignment and synteny analysis revealed that Pofigla was conserved with the orthologous gene sequences in other vertebrates. The results of qRT-PCR showed Pofigla was maternally inherited during embryonic development. For tissue distribution, Pofigla showed a sexually dimorphic gene expression in the gonad of different genders, with a higher expression in ovary than in testis. In situ hybridization(ISH) results demonstrated Pofigla was specifically expressed in germ cells including oocytes, spermatogonia and spermatocytes. By screening and analyzing two proximal regions(-2966/-2126 and-772/-444) with high promoter activity, we found SOX5, LEF1, FOXP1 and GATA1 may play important roles in the transcriptional regulation of Pofigla. Furthermore, we observed the co-localization between Figla and LEF1 in HEK 293T cells. And the significant up-regulation effect of the canonical Wnt signaling pathway on the expression of Pofigla was found in cultured ovarian cells. This study provided the first evidence that figla not only has an important function in ovary development, but also plays some potential roles in testis development and/or male germ cell differentiation during early testis development in P. olivaceus. The results provide valuable reference in exploring the regulatory network of figla in teleost.
文摘利用real-time PCR和原位杂交技术分析了Figla(factor in the germline alpha)基因在湘西黄牛卵巢中的相对表达量与定位表达情况。结果显示:能冲出较多优质胚胎的湘西黄牛个体,卵巢组织中Figla基因mRNA水平的表达量要显著高于对照组(P<0.05)。Figla基因mRNA在牛卵巢组织中的初级卵泡、次级卵泡和成熟的有腔卵泡都有表达。而且Figla基因在有腔卵泡的卵母细胞中的胞质中表达,但在细胞核中没有表达。结果说明:湘西黄牛超数排卵和冲胚效果与卵巢Figla基因表达水平之间存在一定的关系,Figla基因可能是卵泡和早期胚胎发育的双重关键基因。
