Both unit and integration testing are incredibly crucial for almost any software application because each of them operates a distinct process to examine the product.Due to resource constraints,when software is subject...Both unit and integration testing are incredibly crucial for almost any software application because each of them operates a distinct process to examine the product.Due to resource constraints,when software is subjected to modifications,the drastic increase in the count of test cases forces the testers to opt for a test optimization strategy.One such strategy is test case prioritization(TCP).Existing works have propounded various methodologies that re-order the system-level test cases intending to boost either the fault detection capabilities or the coverage efficacy at the earliest.Nonetheless,singularity in objective functions and the lack of dissimilitude among the re-ordered test sequences have degraded the cogency of their approaches.Considering such gaps and scenarios when the meteoric and continuous updations in the software make the intensive unit and integration testing process more fragile,this study has introduced a memetics-inspired methodology for TCP.The proposed structure is first embedded with diverse parameters,and then traditional steps of the shuffled-frog-leaping approach(SFLA)are followed to prioritize the test cases at unit and integration levels.On 5 standard test functions,a comparative analysis is conducted between the established algorithms and the proposed approach,where the latter enhances the coverage rate and fault detection of re-ordered test sets.Investigation results related to the mean average percentage of fault detection(APFD)confirmed that the proposed approach exceeds the memetic,basic multi-walk,PSO,and optimized multi-walk by 21.7%,13.99%,12.24%,and 11.51%,respectively.展开更多
In airborne gamma ray spectrum processing,different analysis methods,technical requirements,analysis models,and calculation methods need to be established.To meet the engineering practice requirements of airborne gamm...In airborne gamma ray spectrum processing,different analysis methods,technical requirements,analysis models,and calculation methods need to be established.To meet the engineering practice requirements of airborne gamma-ray measurements and improve computational efficiency,an improved shuffled frog leaping algorithm-particle swarm optimization convolutional neural network(SFLA-PSO CNN)for large-sample quantitative analysis of airborne gamma-ray spectra is proposed herein.This method was used to train the weight of the neural network,optimize the structure of the network,delete redundant connections,and enable the neural network to acquire the capability of quantitative spectrum processing.In full-spectrum data processing,this method can perform the functions of energy spectrum peak searching and peak area calculations.After network training,the mean SNR and RMSE of the spectral lines were 31.27 and 2.75,respectively,satisfying the demand for noise reduction.To test the processing ability of the algorithm in large samples of airborne gamma spectra,this study considered the measured data from the Saihangaobi survey area as an example to conduct data spectral analysis.The results show that calculation of the single-peak area takes only 0.13~0.15 ms,and the average relative errors of the peak area in the U,Th,and K spectra are 3.11,9.50,and 6.18%,indicating the high processing efficiency and accuracy of this algorithm.The performance of the model can be further improved by optimizing related parameters,but it can already meet the requirements of practical engineering measurement.This study provides a new idea for the full-spectrum processing of airborne gamma rays.展开更多
The dynamic weapon target assignment(DWTA)problem is of great significance in modern air combat.However,DWTA is a highly complex constrained multi-objective combinatorial optimization problem.An improved elitist non-d...The dynamic weapon target assignment(DWTA)problem is of great significance in modern air combat.However,DWTA is a highly complex constrained multi-objective combinatorial optimization problem.An improved elitist non-dominated sorting genetic algorithm-II(NSGA-II)called the non-dominated shuffled frog leaping algorithm(NSFLA)is proposed to maximize damage to enemy targets and minimize the self-threat in air combat constraints.In NSFLA,the shuffled frog leaping algorithm(SFLA)is introduced to NSGA-II to replace the inside evolutionary scheme of the genetic algorithm(GA),displaying low optimization speed and heterogeneous space search defects.Two improvements have also been raised to promote the internal optimization performance of SFLA.Firstly,the local evolution scheme,a novel crossover mechanism,ensures that each individual participates in updating instead of only the worst ones,which can expand the diversity of the population.Secondly,a discrete adaptive mutation algorithm based on the function change rate is applied to balance the global and local search.Finally,the scheme is verified in various air combat scenarios.The results show that the proposed NSFLA has apparent advantages in solution quality and efficiency,especially in many aircraft and the dynamic air combat environment.展开更多
Literary translation should not solely focus on“what to translate”,but also on“how to translate”,extending the perspective to the target audience and environment.It is crucial to recognize that translation involve...Literary translation should not solely focus on“what to translate”,but also on“how to translate”,extending the perspective to the target audience and environment.It is crucial to recognize that translation involves more than rendering words;it entails navigating cultural differences and facilitating literary communication during the process of language conversion.By comparing and contrasting Mo Yan’s work of“蛙”with Howard Goldblatt’s English translation Frog from both the two aspects of language and culture and the four perspectives of alienation and naturalization,deletion and addition,processing of address and dialogues,symbols of animal cultural,this study explores the phenomenon of creative treason in literary translation,and provides a specific and in-depth analysis to offer valuable insights for the translation of Chinese literature.展开更多
The Celebrated Jumping Frog of Calaveras County is a novel first published in 1865.It tells us a story taking place in the ancient mining camp of Angel's.By using analysis,the implication hidden behind the novel i...The Celebrated Jumping Frog of Calaveras County is a novel first published in 1865.It tells us a story taking place in the ancient mining camp of Angel's.By using analysis,the implication hidden behind the novel is explored here.That is,the abnormal social phenomena exist in the reality,the intrigue among people to gain profits and also the immoral measures people took for panning gold at the Gold Rush Era.展开更多
Generally, the function of vocalizations made by male anurans are to attract females or defend resources. Typically, males vocalize in choruses during one or more periods in a twenty-four-hour cycle, which varies, how...Generally, the function of vocalizations made by male anurans are to attract females or defend resources. Typically, males vocalize in choruses during one or more periods in a twenty-four-hour cycle, which varies, however, among species. Nevertheless, the causal factors influencing circadian variations of calling patterns in anuran species are not clear. In this study, male chorus vocalizations were monitored in the Emei music frog(Babina daunchina) for 17 consecutive days during the breeding season, while its habitat air temperature and relative humidity in the course of experiments were measured as well. The results revealed that the circadian calling patterns were characterized by two periods of peak vocalization, which were observed from 0500 h to 0700 h and from 1300 h to 2000 h, while the lowest activity period was found from 2100 h to 2200 h. Both calls/h and notes/h were positively correlated with air temperature and negatively with relative humidity. Overall, our data indicate that the Emei music frogs(B. daunchina) could regulate their vocal activities based on the changes of physical micro-environment(e. g., temperature or humidity) to maximize reproductive success.展开更多
The current study aims to investigate the population variation and food habits of ranid frogs in the rice-based cropping system in District Gujranwala,Pakistan.The population in the study area was estimated using capt...The current study aims to investigate the population variation and food habits of ranid frogs in the rice-based cropping system in District Gujranwala,Pakistan.The population in the study area was estimated using capture,mark and release method whereas food habits of the species were studied by analysis of stomach contents.The results showed the highest average population was found during August 2009(93.10±18.64/ha) while the lowest from December 2008 to February 2009.Maximum seasonal populations existed in summer 2009,whereas winter 2008 sizes were at a minimum.Stomach content analysis of the species revealed percent frequency(% F) of occurrence of insects(80.3),earthworms(28.5),whole frogs(15.8),bone pieces(22.5),rodents(1.66),vegetation(5.0),soil particles(13.3) and some unidentified material(7.5) in all the stomach samples.Most frequently consumed prey items were insects(30% by volume),although frogs also preyed upon conspecifics and rodents.Insects recovered from the stomach contents were identified as belonging to Orthoptera,Lepidoptera,Coleoptera,Diptera,Odonata and Homoptera as well as the class Archnida.Insects recovered from the stomach contents were compared to those captured from the study area.展开更多
To solve the flight control problem for unmanned hypersonic vehicles,a novel intelligent optimized control method is proposed.A flight control system based on integral separated proportional-integral-derivative(PID)co...To solve the flight control problem for unmanned hypersonic vehicles,a novel intelligent optimized control method is proposed.A flight control system based on integral separated proportional-integral-derivative(PID)control is designed for hypersonic vehicle,and an improved shuffled frog leaping algorithm is presented to optimize the control parameters.A nonlinear model of hypersonic vehicle is established to examine the dynamic characteristics achieved by the flight control system.Simulation results demonstrate that the proposed optimized controller can effectively achieve better flight control performance than the traditional controller.展开更多
In the recent restructured power system scenario and complex market strategy, operation at absolute minimum cost is no longer the only criterion for dispatching electric power. The economic load dispatch (ELD) problem...In the recent restructured power system scenario and complex market strategy, operation at absolute minimum cost is no longer the only criterion for dispatching electric power. The economic load dispatch (ELD) problem which accounts for minimization of both generation cost and power loss is itself a multiple conflicting objective function problem. In this paper, a modified shuffled frog-leaping algorithm (MSFLA), which is an improved version of memetic algorithm, is proposed for solving the ELD problem. It is a relatively new evolutionary method where local search is applied during the evolutionary cycle. The idea of memetic algorithm comes from memes, which unlike genes can adapt themselves. The performance of MSFLA has been shown more efficient than traditional evolutionary algorithms for such type of ELD problem. The application and validity of the proposed algorithm are demonstrated for IEEE 30 bus test system as well as a practical power network of 203 bus 264 lines 23 machines system.展开更多
Brain asymmetry for processing visual information is widespread in animals.However,it is still unknown how the complexity of the underlying neural network activities represents this asymmetrical pattern in the brain.I...Brain asymmetry for processing visual information is widespread in animals.However,it is still unknown how the complexity of the underlying neural network activities represents this asymmetrical pattern in the brain.In the present study,we investigated this complexity using the approximate entropy(ApEn)protocol for electroencephalogram(EEG)recordings from the forebrain and midbrain while the music frogs(Nidirana daunchina)attacked prey stimulus.The results showed that(1)more significant prey responses were evoked by the prey stimulus presented in the right visual field than that in the left visual field,consistent with the idea that right-eye preferences for predatory behaviors exist in animals including anurans;(2)in general,the ApEn value of the left hemisphere(especially the left mesencephalon)was greatest under various stimulus conditions,suggesting that visual lateralization could be reflected by the dynamics of underlying neural network activities and that the stable left-hemisphere dominance of EEG ApEn may play an important role in maintaining this brain asymmetry.展开更多
Male-male vocal competition is critical for mating success in anuran species;however,it remains unknown that how males regulate their competitive strategies dynamically during competition because calling is highly tim...Male-male vocal competition is critical for mating success in anuran species;however,it remains unknown that how males regulate their competitive strategies dynamically during competition because calling is highly time-consuming,energetically demanding and likely to increase predation risks.Since different parts of calls will encode different information for vocal communication,we hypothesized that competitive strategies of male frogs may be modulated by the temporal and spectral features of different call notes.To test this hypothesis,the natural advertisement calls(OC),its modified versions with the first call note replaced by white noise(WN)or other notes and with the fifth call note replaced by WN,were played back to the Anhui tree frogs(Rhacophorus zhoukaiyae).Results showed that 1)males produced more competitive calls in response to acoustic stimuli compared to their baseline calling during silence;and 2)males emitted more non-overlapping calls compared to overlapping calls in response to the acoustic stimuli.These results are consistent with the idea that males are flexible to acoustic signals and their competition strategies are modulated dynamically by social contexts.展开更多
Various kinds of biologically active peptides have previously been isolated from the skin secretions of Amolops loloensis frog,such as antimicrobial peptides,bradykinin-like peptides and algesic peptides.A novel insul...Various kinds of biologically active peptides have previously been isolated from the skin secretions of Amolops loloensis frog,such as antimicrobial peptides,bradykinin-like peptides and algesic peptides.A novel insulinotropic peptide named amolopin was identified in A.loloensis frog’s skin secretion.Its primary structure sequence was determined by Edman degradation as:FLPIVGKSLSGLSGKL-NH2.BLAST search indicates that the amino acid sequence of amolopin is quite different from other known insulin secretagogues,including mastoparan,exendins and a-latrotoxin,nor does it like incretins(e.g.glucagons like peptide-1 and glucose-dependent insulinotropic ploypeptide)either.However,amolopin shows certain structural similarity with amphibian antimicrobial temporins and vespid chemotactic peptides isolated from Vespa magnifica.Amolopin can stimulate insulin release in INS-1 cells in a dose-dependent manner.Primary investigation on its action mechanisms reveals that amolopin does not increase the influx of Ca2?.In conclusion,a novel 16-amino acid peptide with insulin-releasing activity is initially discovered from the skin secretion of A.loloensis frog.Further work is necessary to evaluate its potential as novel anti-diabetic candidate.展开更多
Herein, we present the results of our experimental investigation of splashes formed by a frog diving into water from the ground or from a leaf and the accompanying sound generated by the impact of the frog on the wate...Herein, we present the results of our experimental investigation of splashes formed by a frog diving into water from the ground or from a leaf and the accompanying sound generated by the impact of the frog on the water. The experiments are performed by visualizing the flow with a high-speed camera. In addition, we used physical models comprising hydrophilic bodies made from hydrogel or acrylic resin to experimentally study how hydrophilicity affects the splash. In these experiments, we use the degree of swelling to define the hydrophilicity degree. The results show that different splashes are caused by the increase in water-film velocity upon an increase in hydrophilicity. For a body with strong hydrophilicity, at a relatively high film velocity, the water film forms when the body impacts the water surface separates from the body surface. In addition, an aircavity forms when the film separates from the body. We empirically examine the relation between the hydrophilicity degree and film velocity. The results indicate that increased hydrophilicity does not reduce the splash. Therefore, we conclude that reducing of the formation of water from the biomimetic point of view is related to the shape of body.展开更多
Fifteen microsatellite DNA markers were developed from a transcriptome assembly of the alpine stream frog(Scutiger boulengeri).To characterize these loci, we genotyped 23 individuals collected from two sites. Eleven l...Fifteen microsatellite DNA markers were developed from a transcriptome assembly of the alpine stream frog(Scutiger boulengeri).To characterize these loci, we genotyped 23 individuals collected from two sites. Eleven loci were variable, with the number of alleles ranging from one to six within each population. The expected and observed heterozygosities ranged from 0 to 0.78 and from 0 to 0.64, respectively. None of the loci was in linkage disequilibrium and one locus for one population was deviated from the Hardy-Weinberg equilibrium. We hope that these markers will offer useful tools in detecting population structure of S. boulengeri and in monitoring the fragile ecosystem of the Tibetan Plateau, where the species resides.展开更多
文摘Both unit and integration testing are incredibly crucial for almost any software application because each of them operates a distinct process to examine the product.Due to resource constraints,when software is subjected to modifications,the drastic increase in the count of test cases forces the testers to opt for a test optimization strategy.One such strategy is test case prioritization(TCP).Existing works have propounded various methodologies that re-order the system-level test cases intending to boost either the fault detection capabilities or the coverage efficacy at the earliest.Nonetheless,singularity in objective functions and the lack of dissimilitude among the re-ordered test sequences have degraded the cogency of their approaches.Considering such gaps and scenarios when the meteoric and continuous updations in the software make the intensive unit and integration testing process more fragile,this study has introduced a memetics-inspired methodology for TCP.The proposed structure is first embedded with diverse parameters,and then traditional steps of the shuffled-frog-leaping approach(SFLA)are followed to prioritize the test cases at unit and integration levels.On 5 standard test functions,a comparative analysis is conducted between the established algorithms and the proposed approach,where the latter enhances the coverage rate and fault detection of re-ordered test sets.Investigation results related to the mean average percentage of fault detection(APFD)confirmed that the proposed approach exceeds the memetic,basic multi-walk,PSO,and optimized multi-walk by 21.7%,13.99%,12.24%,and 11.51%,respectively.
基金the National Natural Science Foundation of China(No.42127807)Natural Science Foundation of Sichuan Province(Nos.23NSFSCC0116 and 2022NSFSC12333)the Nuclear Energy Development Project(No.[2021]-88).
文摘In airborne gamma ray spectrum processing,different analysis methods,technical requirements,analysis models,and calculation methods need to be established.To meet the engineering practice requirements of airborne gamma-ray measurements and improve computational efficiency,an improved shuffled frog leaping algorithm-particle swarm optimization convolutional neural network(SFLA-PSO CNN)for large-sample quantitative analysis of airborne gamma-ray spectra is proposed herein.This method was used to train the weight of the neural network,optimize the structure of the network,delete redundant connections,and enable the neural network to acquire the capability of quantitative spectrum processing.In full-spectrum data processing,this method can perform the functions of energy spectrum peak searching and peak area calculations.After network training,the mean SNR and RMSE of the spectral lines were 31.27 and 2.75,respectively,satisfying the demand for noise reduction.To test the processing ability of the algorithm in large samples of airborne gamma spectra,this study considered the measured data from the Saihangaobi survey area as an example to conduct data spectral analysis.The results show that calculation of the single-peak area takes only 0.13~0.15 ms,and the average relative errors of the peak area in the U,Th,and K spectra are 3.11,9.50,and 6.18%,indicating the high processing efficiency and accuracy of this algorithm.The performance of the model can be further improved by optimizing related parameters,but it can already meet the requirements of practical engineering measurement.This study provides a new idea for the full-spectrum processing of airborne gamma rays.
基金supported by the National Natural Science Foundation of China(61673209,71971115)。
文摘The dynamic weapon target assignment(DWTA)problem is of great significance in modern air combat.However,DWTA is a highly complex constrained multi-objective combinatorial optimization problem.An improved elitist non-dominated sorting genetic algorithm-II(NSGA-II)called the non-dominated shuffled frog leaping algorithm(NSFLA)is proposed to maximize damage to enemy targets and minimize the self-threat in air combat constraints.In NSFLA,the shuffled frog leaping algorithm(SFLA)is introduced to NSGA-II to replace the inside evolutionary scheme of the genetic algorithm(GA),displaying low optimization speed and heterogeneous space search defects.Two improvements have also been raised to promote the internal optimization performance of SFLA.Firstly,the local evolution scheme,a novel crossover mechanism,ensures that each individual participates in updating instead of only the worst ones,which can expand the diversity of the population.Secondly,a discrete adaptive mutation algorithm based on the function change rate is applied to balance the global and local search.Finally,the scheme is verified in various air combat scenarios.The results show that the proposed NSFLA has apparent advantages in solution quality and efficiency,especially in many aircraft and the dynamic air combat environment.
文摘Literary translation should not solely focus on“what to translate”,but also on“how to translate”,extending the perspective to the target audience and environment.It is crucial to recognize that translation involves more than rendering words;it entails navigating cultural differences and facilitating literary communication during the process of language conversion.By comparing and contrasting Mo Yan’s work of“蛙”with Howard Goldblatt’s English translation Frog from both the two aspects of language and culture and the four perspectives of alienation and naturalization,deletion and addition,processing of address and dialogues,symbols of animal cultural,this study explores the phenomenon of creative treason in literary translation,and provides a specific and in-depth analysis to offer valuable insights for the translation of Chinese literature.
文摘The Celebrated Jumping Frog of Calaveras County is a novel first published in 1865.It tells us a story taking place in the ancient mining camp of Angel's.By using analysis,the implication hidden behind the novel is explored here.That is,the abnormal social phenomena exist in the reality,the intrigue among people to gain profits and also the immoral measures people took for panning gold at the Gold Rush Era.
基金supported by the National Natural Science Foundation of China(30900141)the Chinese Academy of Sciences(KSCX2-EW-J-22,09C302,Y0B3011)the Chinese Academy of Sciences(KSCX2-YW-R-077)
文摘Generally, the function of vocalizations made by male anurans are to attract females or defend resources. Typically, males vocalize in choruses during one or more periods in a twenty-four-hour cycle, which varies, however, among species. Nevertheless, the causal factors influencing circadian variations of calling patterns in anuran species are not clear. In this study, male chorus vocalizations were monitored in the Emei music frog(Babina daunchina) for 17 consecutive days during the breeding season, while its habitat air temperature and relative humidity in the course of experiments were measured as well. The results revealed that the circadian calling patterns were characterized by two periods of peak vocalization, which were observed from 0500 h to 0700 h and from 1300 h to 2000 h, while the lowest activity period was found from 2100 h to 2200 h. Both calls/h and notes/h were positively correlated with air temperature and negatively with relative humidity. Overall, our data indicate that the Emei music frogs(B. daunchina) could regulate their vocal activities based on the changes of physical micro-environment(e. g., temperature or humidity) to maximize reproductive success.
文摘The current study aims to investigate the population variation and food habits of ranid frogs in the rice-based cropping system in District Gujranwala,Pakistan.The population in the study area was estimated using capture,mark and release method whereas food habits of the species were studied by analysis of stomach contents.The results showed the highest average population was found during August 2009(93.10±18.64/ha) while the lowest from December 2008 to February 2009.Maximum seasonal populations existed in summer 2009,whereas winter 2008 sizes were at a minimum.Stomach content analysis of the species revealed percent frequency(% F) of occurrence of insects(80.3),earthworms(28.5),whole frogs(15.8),bone pieces(22.5),rodents(1.66),vegetation(5.0),soil particles(13.3) and some unidentified material(7.5) in all the stomach samples.Most frequently consumed prey items were insects(30% by volume),although frogs also preyed upon conspecifics and rodents.Insects recovered from the stomach contents were identified as belonging to Orthoptera,Lepidoptera,Coleoptera,Diptera,Odonata and Homoptera as well as the class Archnida.Insects recovered from the stomach contents were compared to those captured from the study area.
基金supported in part by the National Natural Science Foundation of China(No.61304223)the Specialized Research Fund for the Doctoral Program of Higher Education(No.20123218120015)the Fundamental Research Funds for the Central Universities(No.NZ2015206)
文摘To solve the flight control problem for unmanned hypersonic vehicles,a novel intelligent optimized control method is proposed.A flight control system based on integral separated proportional-integral-derivative(PID)control is designed for hypersonic vehicle,and an improved shuffled frog leaping algorithm is presented to optimize the control parameters.A nonlinear model of hypersonic vehicle is established to examine the dynamic characteristics achieved by the flight control system.Simulation results demonstrate that the proposed optimized controller can effectively achieve better flight control performance than the traditional controller.
文摘In the recent restructured power system scenario and complex market strategy, operation at absolute minimum cost is no longer the only criterion for dispatching electric power. The economic load dispatch (ELD) problem which accounts for minimization of both generation cost and power loss is itself a multiple conflicting objective function problem. In this paper, a modified shuffled frog-leaping algorithm (MSFLA), which is an improved version of memetic algorithm, is proposed for solving the ELD problem. It is a relatively new evolutionary method where local search is applied during the evolutionary cycle. The idea of memetic algorithm comes from memes, which unlike genes can adapt themselves. The performance of MSFLA has been shown more efficient than traditional evolutionary algorithms for such type of ELD problem. The application and validity of the proposed algorithm are demonstrated for IEEE 30 bus test system as well as a practical power network of 203 bus 264 lines 23 machines system.
基金supported by the grants from the National Natural Science Foundation of China(No.31970422,No.31672305 and No.31372217 to Guangzhan Fang)the Key Research Project of Education Department of Sichuan Province(No.18ZA0321 to Yansu Liu)。
文摘Brain asymmetry for processing visual information is widespread in animals.However,it is still unknown how the complexity of the underlying neural network activities represents this asymmetrical pattern in the brain.In the present study,we investigated this complexity using the approximate entropy(ApEn)protocol for electroencephalogram(EEG)recordings from the forebrain and midbrain while the music frogs(Nidirana daunchina)attacked prey stimulus.The results showed that(1)more significant prey responses were evoked by the prey stimulus presented in the right visual field than that in the left visual field,consistent with the idea that right-eye preferences for predatory behaviors exist in animals including anurans;(2)in general,the ApEn value of the left hemisphere(especially the left mesencephalon)was greatest under various stimulus conditions,suggesting that visual lateralization could be reflected by the dynamics of underlying neural network activities and that the stable left-hemisphere dominance of EEG ApEn may play an important role in maintaining this brain asymmetry.
基金supported by the grants from the National Natural Science Foundation of China(Nos.31970422,31672305 and 31372217 to Guangzhan FANG,No.31572275 to Yezhong TANG)the grants from Biodiversity Conservation Programe of Ministry of Ecology and Environment of China to Baowei ZHANG。
文摘Male-male vocal competition is critical for mating success in anuran species;however,it remains unknown that how males regulate their competitive strategies dynamically during competition because calling is highly time-consuming,energetically demanding and likely to increase predation risks.Since different parts of calls will encode different information for vocal communication,we hypothesized that competitive strategies of male frogs may be modulated by the temporal and spectral features of different call notes.To test this hypothesis,the natural advertisement calls(OC),its modified versions with the first call note replaced by white noise(WN)or other notes and with the fifth call note replaced by WN,were played back to the Anhui tree frogs(Rhacophorus zhoukaiyae).Results showed that 1)males produced more competitive calls in response to acoustic stimuli compared to their baseline calling during silence;and 2)males emitted more non-overlapping calls compared to overlapping calls in response to the acoustic stimuli.These results are consistent with the idea that males are flexible to acoustic signals and their competition strategies are modulated dynamically by social contexts.
文摘Various kinds of biologically active peptides have previously been isolated from the skin secretions of Amolops loloensis frog,such as antimicrobial peptides,bradykinin-like peptides and algesic peptides.A novel insulinotropic peptide named amolopin was identified in A.loloensis frog’s skin secretion.Its primary structure sequence was determined by Edman degradation as:FLPIVGKSLSGLSGKL-NH2.BLAST search indicates that the amino acid sequence of amolopin is quite different from other known insulin secretagogues,including mastoparan,exendins and a-latrotoxin,nor does it like incretins(e.g.glucagons like peptide-1 and glucose-dependent insulinotropic ploypeptide)either.However,amolopin shows certain structural similarity with amphibian antimicrobial temporins and vespid chemotactic peptides isolated from Vespa magnifica.Amolopin can stimulate insulin release in INS-1 cells in a dose-dependent manner.Primary investigation on its action mechanisms reveals that amolopin does not increase the influx of Ca2?.In conclusion,a novel 16-amino acid peptide with insulin-releasing activity is initially discovered from the skin secretion of A.loloensis frog.Further work is necessary to evaluate its potential as novel anti-diabetic candidate.
文摘Herein, we present the results of our experimental investigation of splashes formed by a frog diving into water from the ground or from a leaf and the accompanying sound generated by the impact of the frog on the water. The experiments are performed by visualizing the flow with a high-speed camera. In addition, we used physical models comprising hydrophilic bodies made from hydrogel or acrylic resin to experimentally study how hydrophilicity affects the splash. In these experiments, we use the degree of swelling to define the hydrophilicity degree. The results show that different splashes are caused by the increase in water-film velocity upon an increase in hydrophilicity. For a body with strong hydrophilicity, at a relatively high film velocity, the water film forms when the body impacts the water surface separates from the body surface. In addition, an aircavity forms when the film separates from the body. We empirically examine the relation between the hydrophilicity degree and film velocity. The results indicate that increased hydrophilicity does not reduce the splash. Therefore, we conclude that reducing of the formation of water from the biomimetic point of view is related to the shape of body.
基金supported by a scholarship from the China Scholarship Council to Z.DU
文摘Fifteen microsatellite DNA markers were developed from a transcriptome assembly of the alpine stream frog(Scutiger boulengeri).To characterize these loci, we genotyped 23 individuals collected from two sites. Eleven loci were variable, with the number of alleles ranging from one to six within each population. The expected and observed heterozygosities ranged from 0 to 0.78 and from 0 to 0.64, respectively. None of the loci was in linkage disequilibrium and one locus for one population was deviated from the Hardy-Weinberg equilibrium. We hope that these markers will offer useful tools in detecting population structure of S. boulengeri and in monitoring the fragile ecosystem of the Tibetan Plateau, where the species resides.
