DNA barcoding and HPLC specific chromatogram were used to identify three kinds of Plumeria flowers respectively. DNAs extracted from the three Plumeria species were amplified by PCR with universal primers, and the psb...DNA barcoding and HPLC specific chromatogram were used to identify three kinds of Plumeria flowers respectively. DNAs extracted from the three Plumeria species were amplified by PCR with universal primers, and the psbA-trnH region was selected. All the amplified products were sequenced and the results were analyzed by MEGA 5.0. Chemometric methods including principal components analysis and hierarchical clustering analysis were conducted on the SAS 9.0 software to demonstrate the variability among samples. In conclusion, the psbA-trnH of all samples were successfully amplified from total DNA and sequenced. These three varieties of Plumeria can be differentiated by the psbA-trnH region and clustered into three groups respectively through building neighbor joining tree, which conformed to their germplasm origins. However, it was hard to distinguish them by HPLC specific chromatograms combined with chemometrics analysis. These indicated that DNA barcoding was a promising and reliable tool for the identification of three kinds of Plumeria flowers compared to HPLC specific chromatogram generally used. It could be treated as a powerful complementary method for traditional authentication, especially for those varieties which are difficult to be identified by conventional chromatography.展开更多
AIM: To analyze the major constituents in Radix Scrophulariae(Scrophularia ningpoensis). METHOD: Radix Scrophulariae was analyzed by HPLC coupled with electrospray ionization quadrupole time-of-flight tandem mass spec...AIM: To analyze the major constituents in Radix Scrophulariae(Scrophularia ningpoensis). METHOD: Radix Scrophulariae was analyzed by HPLC coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry(ESI-Q-TOF MS/MS). Compounds were separated by HPLC using a C18 column and gradient elution of acetonitrile and 0.1 %(V/V) acetic acid-water. Negative ion mode was employed. RESULTS: A total of thirty-six compounds, including fourteen iridoid glycosides, nineteen phenylpropanoid glycosides, and three organic acids, were identified from Radix Scrophulariae based on the accurate mass measurement of precursor and product ions. Twenty-one of the constituents were identified by comparing their retention times(tR) and ESI-MS/MS data with those of reference standards and/or previous publications, while another fifteen compounds were tentatively identified or deduced according to their Q-TOF MS/MS data which afforded sufficient structural information. CONCLUSION: It is believed that this study is useful for the identification of constituents in Radix Scrophulariae, as well as related plants and complex prescriptions.展开更多
Abstract： Olive oil adulteration with less expensive edible oils is a major issue for the olive oil industry. Eleven vegetable oils were selected and purchased from different markets in Iraqi Kurdistan region （Erbil...Abstract： Olive oil adulteration with less expensive edible oils is a major issue for the olive oil industry. Eleven vegetable oils were selected and purchased from different markets in Iraqi Kurdistan region （Erbil City center）. In this study high performance liquid chromatography （HPLC） on reversed-phase （RP） column with refractive index （RI） detector was used to classify some components in different edible oils depending on calculation of partition number. Adulteration of one of the olive oil samples （01） was identified, due to the appearance of glycerol trilinoleate （LLL） peak in the HPLC chromatogram, while this peak is not present in HPLC chromatograms of other olive oil samples. To ensure the presence of LLL a standard concentration of LLL was added to the sample and increasing the peak height at the retention time of LLL indicates the presence of LLL and the adulteration of olive oil sample.展开更多
Furniture of Hongmu,cream of Chinese culture,has a long history and enjoys great popularity for its classic appearance and elegant disposition. The traditional method of the identification is to evaluate the macro for...Furniture of Hongmu,cream of Chinese culture,has a long history and enjoys great popularity for its classic appearance and elegant disposition. The traditional method of the identification is to evaluate the macro form and anatomical features.In this paper,a new method based on the differences among the chemical characteristics of biological metabolites of samples is developed.In the research, high performance liquid chromatography(HPLC) fingerprints were employed to discriminate four species of Hongmu,including Pterocarpus indicus, Pterocarpus macarocarpus,Dalbergia melanoxylon, and Dalbergia louvelii.The results showed that, according to the retention time and relative peak area of different components in wood extracts,the distinction can be easily made between different species.Based on the HPLC fingerprints,similarities between samples were evaluated by correlation coefficients.The results were greater than 0.81 between the same species,and less than 0.21 between different species,which showed weak correlation between different species.In order to estimate the overall features of the fingerprints, hierarchical clustering and principal component analysis(PCA) were used and the results showed pronounced clustering effect among the same species.The investigation indicated that pattern recognition could accurately reflect the significant differences between different species of Hongmu, and can be used as a reference for quality control.展开更多
以6种不同品种的蓝莓果实(埃利奥特、爱国者、北蓝、北陆、杜克、蓝丰)为原料,利用甲醇水溶液提取蓝莓中的花青素。用二极管阵列-高效液相色谱-电喷雾离子阱飞行时间串联多级质谱仪(High performance liquid chromatography-electric sp...以6种不同品种的蓝莓果实(埃利奥特、爱国者、北蓝、北陆、杜克、蓝丰)为原料,利用甲醇水溶液提取蓝莓中的花青素。用二极管阵列-高效液相色谱-电喷雾离子阱飞行时间串联多级质谱仪(High performance liquid chromatography-electric spray four pole flight time series multipolar mass spectrometer,HPLC-DAD-ESI/IT-TOF/MSn)及紫外可见光谱及多级质谱数据对得到的6种不同品种的蓝莓花青素结构进行鉴定和对比。结果表明,6种蓝莓果实中共鉴定出13种花青素类化合物,苷元分别为锦葵色素、花翠素、矢车菊素、牵牛花色素、芍药素。其中埃利奥特蓝莓检测到11种花青素,爱国者10种,北蓝7种,北陆12种,杜克9种,蓝丰6种。从花青素结构上分析,6种蓝莓均可检测到带糖配基的锦葵色素-3-O-半乳糖、不连接糖苷的花翠素及矢车菊素;不同的蓝莓品种间所含有的花青素种类、个数、结构等均有明显差异。本研究为快速获取蓝莓中花青素的结构信息提供一定的理论支撑。展开更多
基于化学识别模式,建立HPLC指纹图谱及多成分含量测定,为金花三宝口服液的质量评价提供参考。采用HPLC法,以Capcell Pak C_(18)(250mm×4.6mm,5μm)为色谱柱,流速为0.8mL·min^(-1),流动相是甲醇(A)-0.1%磷酸水(B),柱温为30℃,...基于化学识别模式,建立HPLC指纹图谱及多成分含量测定,为金花三宝口服液的质量评价提供参考。采用HPLC法,以Capcell Pak C_(18)(250mm×4.6mm,5μm)为色谱柱,流速为0.8mL·min^(-1),流动相是甲醇(A)-0.1%磷酸水(B),柱温为30℃,进样量为10μL,检测波长为266nm;建立指纹图谱并同时测定6个成分的含量,采用聚类分析(CA),主成分分析(PCA)以及偏最小二乘-判别分析(PLS-DA)对数据进行分析。指纹图谱共确定了13个共有峰,并指认了6个成分;相似度值为0.958以上;聚类分析,主成分分析将样品划分为2类,偏最小二乘-判别分析筛选出峰12,新绿原酸,芦丁等8个质量差异标志成分。含量测定结果表明:10批金花三宝口服液中新绿原酸、绿原酸、隐绿原酸、异绿原酸A、芦丁、异绿原酸C的平均含量依次为:0.2183,0.8465,0.1644,0.3790,0.0749,0.1701mg·mL^(-1)。本研究所建立的HPLC指纹图谱及多成分的含量测定准确,稳定可行,可为质量控制提供参考依据。展开更多
Objective：Root or rhizome of Panax notoginseng（Sanqi）is known for its eutherapeutic effects.Panax vietnamensis var.fuscidicus,called Yesanqi or Yuenan sanqi by local residents,is also commercially available.They ar...Objective：Root or rhizome of Panax notoginseng（Sanqi）is known for its eutherapeutic effects.Panax vietnamensis var.fuscidicus,called Yesanqi or Yuenan sanqi by local residents,is also commercially available.They are similar in morphology,leading to serious safety problems in clinical medication.It is necessary to find the rapid and efficient methods to identify them.Methods：P.notoginseng and P.vietnamensis var.fuscidicus were identified by DNA barcoding based on the ITS2 sequence.Notoginsenoside R1 and ginsenosides（Rb1,Rg1,Re,Rd,Rc,and Rb2）were analyzed in the roots,fibrils,stems,leaves,and flowers of P.notoginseng and P.vietnamensis var.fuscidicus using high-performance liquid chromatography（HPLC）.Results：P.notoginseng and P.vietnamensis var.fuscidicus were separated into branches of divergent clusters,and P.vietnamensis var.fuscidicus and Panax vietnamensis were clustered into a clade with 98%similarity according to DNA barcoding analysis.The chemical compositions of P.notoginseng and P.vietnamensis var.fuscidicus were similar in roots;while their compositions and contents of notoginsenoside R1 and ginsenosides in flowers,leaves,stems,and fibrils were different.Conclusion：ITS2 is a rapid and efficient method to identify P.notoginseng and P.vietnamensis var.fuscidicus.HPLC analysis indicated that pharmacological action might be different between P.notoginseng and P.vietnamensis var.fuscidicus.展开更多
The root of Hedysarum multijugum（RHM） is recorded as a folk herbal medicine in China and is sometimes used as a substitute for Hedysari Radix, which is a famous traditional Chinese medicine derived from the roots of...The root of Hedysarum multijugum（RHM） is recorded as a folk herbal medicine in China and is sometimes used as a substitute for Hedysari Radix, which is a famous traditional Chinese medicine derived from the roots of Hedysarum polybotrys. In the present study, a sensible, reliable, and reproducible HPLC-DAD fingerprint analysis method for RHM was developed and then subsequently applied to analyze RHM samples from different origins. The chemical constituents of the RHM samples were generally consistent, although it was slightly affected by the local environment of the plant. In addition, the chemical constituency of RHM was shown to be significantly different from that of Hedysari Radix, suggesting that RHM is not suitable as a substitute for Hedysari Radix, at least from the chemical point of view.展开更多
HPLC fingerprinting and determination methods were applied to evaluate 88 batches of Rhizoma Coptidis from 27 different areas. The results showed that HPLC fingerprint of Rhizoma Coptidis was established with good sep...HPLC fingerprinting and determination methods were applied to evaluate 88 batches of Rhizoma Coptidis from 27 different areas. The results showed that HPLC fingerprint of Rhizoma Coptidis was established with good separation and repeatability, which could be used for quality assessment of Rhizoma Coptidis. Twelve common peaks were defined in characteristic fingerprints, and similarity evaluation system was applied to evaluate the fingerprints of 88 batches of Rhizoma Coptidis, the contents of six alkaloids have been determined. The method of assessing Rhizoma Coptidis and evaluation of its quality has been established.展开更多
传统的通信信号调制识别方法容易出现过拟合或欠拟合等问题,导致识别结果误差率较高。为了降低误差率、提高准确率,提出基于改进蚁群算法的高速电力线载波(High-speed Power Line Carrier,HPLC)通信信号调制识别方法。先通过计算幅度谱...传统的通信信号调制识别方法容易出现过拟合或欠拟合等问题,导致识别结果误差率较高。为了降低误差率、提高准确率,提出基于改进蚁群算法的高速电力线载波(High-speed Power Line Carrier,HPLC)通信信号调制识别方法。先通过计算幅度谱密度最大值、相位绝对值的标准偏差、频率绝对值的标准偏差,提取通信信号的特征参数,然后基于改进蚁群算法构建信号调制识别模型,实现HPLC通信信号的调制识别。设计对比实验,实验结果证明该方法在对通信信号调制识别准确率的提升方面具有显著效果,更符合实际的数字信号识别需要。在实际应用中,该方法可以广泛应用于各种需要快速、准确识别通信信号的领域。展开更多
基金supported by the Industry-University-Research Cooperation Program from Science and Technology Department of Guangdong Province (No.: 2013B090600058)the National Key Research and Development Program of China (2017YFC170116)
文摘DNA barcoding and HPLC specific chromatogram were used to identify three kinds of Plumeria flowers respectively. DNAs extracted from the three Plumeria species were amplified by PCR with universal primers, and the psbA-trnH region was selected. All the amplified products were sequenced and the results were analyzed by MEGA 5.0. Chemometric methods including principal components analysis and hierarchical clustering analysis were conducted on the SAS 9.0 software to demonstrate the variability among samples. In conclusion, the psbA-trnH of all samples were successfully amplified from total DNA and sequenced. These three varieties of Plumeria can be differentiated by the psbA-trnH region and clustered into three groups respectively through building neighbor joining tree, which conformed to their germplasm origins. However, it was hard to distinguish them by HPLC specific chromatograms combined with chemometrics analysis. These indicated that DNA barcoding was a promising and reliable tool for the identification of three kinds of Plumeria flowers compared to HPLC specific chromatogram generally used. It could be treated as a powerful complementary method for traditional authentication, especially for those varieties which are difficult to be identified by conventional chromatography.
基金supported by the National Natural Science Foundation of China(Nos.30901956,30973965)2011’Program for Excellent Scientific and Technological Innovation Team of Jiangsu Higher Education and the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘AIM: To analyze the major constituents in Radix Scrophulariae(Scrophularia ningpoensis). METHOD: Radix Scrophulariae was analyzed by HPLC coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry(ESI-Q-TOF MS/MS). Compounds were separated by HPLC using a C18 column and gradient elution of acetonitrile and 0.1 %(V/V) acetic acid-water. Negative ion mode was employed. RESULTS: A total of thirty-six compounds, including fourteen iridoid glycosides, nineteen phenylpropanoid glycosides, and three organic acids, were identified from Radix Scrophulariae based on the accurate mass measurement of precursor and product ions. Twenty-one of the constituents were identified by comparing their retention times(tR) and ESI-MS/MS data with those of reference standards and/or previous publications, while another fifteen compounds were tentatively identified or deduced according to their Q-TOF MS/MS data which afforded sufficient structural information. CONCLUSION: It is believed that this study is useful for the identification of constituents in Radix Scrophulariae, as well as related plants and complex prescriptions.
文摘Abstract： Olive oil adulteration with less expensive edible oils is a major issue for the olive oil industry. Eleven vegetable oils were selected and purchased from different markets in Iraqi Kurdistan region （Erbil City center）. In this study high performance liquid chromatography （HPLC） on reversed-phase （RP） column with refractive index （RI） detector was used to classify some components in different edible oils depending on calculation of partition number. Adulteration of one of the olive oil samples （01） was identified, due to the appearance of glycerol trilinoleate （LLL） peak in the HPLC chromatogram, while this peak is not present in HPLC chromatograms of other olive oil samples. To ensure the presence of LLL a standard concentration of LLL was added to the sample and increasing the peak height at the retention time of LLL indicates the presence of LLL and the adulteration of olive oil sample.
文摘Furniture of Hongmu,cream of Chinese culture,has a long history and enjoys great popularity for its classic appearance and elegant disposition. The traditional method of the identification is to evaluate the macro form and anatomical features.In this paper,a new method based on the differences among the chemical characteristics of biological metabolites of samples is developed.In the research, high performance liquid chromatography(HPLC) fingerprints were employed to discriminate four species of Hongmu,including Pterocarpus indicus, Pterocarpus macarocarpus,Dalbergia melanoxylon, and Dalbergia louvelii.The results showed that, according to the retention time and relative peak area of different components in wood extracts,the distinction can be easily made between different species.Based on the HPLC fingerprints,similarities between samples were evaluated by correlation coefficients.The results were greater than 0.81 between the same species,and less than 0.21 between different species,which showed weak correlation between different species.In order to estimate the overall features of the fingerprints, hierarchical clustering and principal component analysis(PCA) were used and the results showed pronounced clustering effect among the same species.The investigation indicated that pattern recognition could accurately reflect the significant differences between different species of Hongmu, and can be used as a reference for quality control.
文摘以6种不同品种的蓝莓果实(埃利奥特、爱国者、北蓝、北陆、杜克、蓝丰)为原料,利用甲醇水溶液提取蓝莓中的花青素。用二极管阵列-高效液相色谱-电喷雾离子阱飞行时间串联多级质谱仪(High performance liquid chromatography-electric spray four pole flight time series multipolar mass spectrometer,HPLC-DAD-ESI/IT-TOF/MSn)及紫外可见光谱及多级质谱数据对得到的6种不同品种的蓝莓花青素结构进行鉴定和对比。结果表明,6种蓝莓果实中共鉴定出13种花青素类化合物,苷元分别为锦葵色素、花翠素、矢车菊素、牵牛花色素、芍药素。其中埃利奥特蓝莓检测到11种花青素,爱国者10种,北蓝7种,北陆12种,杜克9种,蓝丰6种。从花青素结构上分析,6种蓝莓均可检测到带糖配基的锦葵色素-3-O-半乳糖、不连接糖苷的花翠素及矢车菊素;不同的蓝莓品种间所含有的花青素种类、个数、结构等均有明显差异。本研究为快速获取蓝莓中花青素的结构信息提供一定的理论支撑。
文摘基于化学识别模式,建立HPLC指纹图谱及多成分含量测定,为金花三宝口服液的质量评价提供参考。采用HPLC法,以Capcell Pak C_(18)(250mm×4.6mm,5μm)为色谱柱,流速为0.8mL·min^(-1),流动相是甲醇(A)-0.1%磷酸水(B),柱温为30℃,进样量为10μL,检测波长为266nm;建立指纹图谱并同时测定6个成分的含量,采用聚类分析(CA),主成分分析(PCA)以及偏最小二乘-判别分析(PLS-DA)对数据进行分析。指纹图谱共确定了13个共有峰,并指认了6个成分;相似度值为0.958以上;聚类分析,主成分分析将样品划分为2类,偏最小二乘-判别分析筛选出峰12,新绿原酸,芦丁等8个质量差异标志成分。含量测定结果表明:10批金花三宝口服液中新绿原酸、绿原酸、隐绿原酸、异绿原酸A、芦丁、异绿原酸C的平均含量依次为:0.2183,0.8465,0.1644,0.3790,0.0749,0.1701mg·mL^(-1)。本研究所建立的HPLC指纹图谱及多成分的含量测定准确,稳定可行,可为质量控制提供参考依据。
基金supported by the grants from the National Natural Science Foundation of China(No.81603238)Beijing Nova Project(No.xx2018014)the Major Science and Technology Programs in Yunnan Province(No.2016ZF001-001)
文摘Objective：Root or rhizome of Panax notoginseng（Sanqi）is known for its eutherapeutic effects.Panax vietnamensis var.fuscidicus,called Yesanqi or Yuenan sanqi by local residents,is also commercially available.They are similar in morphology,leading to serious safety problems in clinical medication.It is necessary to find the rapid and efficient methods to identify them.Methods：P.notoginseng and P.vietnamensis var.fuscidicus were identified by DNA barcoding based on the ITS2 sequence.Notoginsenoside R1 and ginsenosides（Rb1,Rg1,Re,Rd,Rc,and Rb2）were analyzed in the roots,fibrils,stems,leaves,and flowers of P.notoginseng and P.vietnamensis var.fuscidicus using high-performance liquid chromatography（HPLC）.Results：P.notoginseng and P.vietnamensis var.fuscidicus were separated into branches of divergent clusters,and P.vietnamensis var.fuscidicus and Panax vietnamensis were clustered into a clade with 98%similarity according to DNA barcoding analysis.The chemical compositions of P.notoginseng and P.vietnamensis var.fuscidicus were similar in roots;while their compositions and contents of notoginsenoside R1 and ginsenosides in flowers,leaves,stems,and fibrils were different.Conclusion：ITS2 is a rapid and efficient method to identify P.notoginseng and P.vietnamensis var.fuscidicus.HPLC analysis indicated that pharmacological action might be different between P.notoginseng and P.vietnamensis var.fuscidicus.
基金Quality Standards for Chinese Medicines of Chinese Pharmacopeia 2010 edition(Grant No.YZ-029)National Natural Science Foundation of China(Grant No.21372015)
文摘The root of Hedysarum multijugum（RHM） is recorded as a folk herbal medicine in China and is sometimes used as a substitute for Hedysari Radix, which is a famous traditional Chinese medicine derived from the roots of Hedysarum polybotrys. In the present study, a sensible, reliable, and reproducible HPLC-DAD fingerprint analysis method for RHM was developed and then subsequently applied to analyze RHM samples from different origins. The chemical constituents of the RHM samples were generally consistent, although it was slightly affected by the local environment of the plant. In addition, the chemical constituency of RHM was shown to be significantly different from that of Hedysari Radix, suggesting that RHM is not suitable as a substitute for Hedysari Radix, at least from the chemical point of view.
基金Special Scientific Research of Chinese Medicine Industry(Grant No 200707014)
文摘HPLC fingerprinting and determination methods were applied to evaluate 88 batches of Rhizoma Coptidis from 27 different areas. The results showed that HPLC fingerprint of Rhizoma Coptidis was established with good separation and repeatability, which could be used for quality assessment of Rhizoma Coptidis. Twelve common peaks were defined in characteristic fingerprints, and similarity evaluation system was applied to evaluate the fingerprints of 88 batches of Rhizoma Coptidis, the contents of six alkaloids have been determined. The method of assessing Rhizoma Coptidis and evaluation of its quality has been established.
文摘传统的通信信号调制识别方法容易出现过拟合或欠拟合等问题,导致识别结果误差率较高。为了降低误差率、提高准确率,提出基于改进蚁群算法的高速电力线载波(High-speed Power Line Carrier,HPLC)通信信号调制识别方法。先通过计算幅度谱密度最大值、相位绝对值的标准偏差、频率绝对值的标准偏差,提取通信信号的特征参数,然后基于改进蚁群算法构建信号调制识别模型,实现HPLC通信信号的调制识别。设计对比实验,实验结果证明该方法在对通信信号调制识别准确率的提升方面具有显著效果,更符合实际的数字信号识别需要。在实际应用中,该方法可以广泛应用于各种需要快速、准确识别通信信号的领域。
