[Objectives]To study the clinical effect of Acupoint Application of Jiezi Fangxiao Ointment combined with Pingchuan Prescription in the treatment of pediatric asthma.[Methods]A total of 40 children with asthma admitte...[Objectives]To study the clinical effect of Acupoint Application of Jiezi Fangxiao Ointment combined with Pingchuan Prescription in the treatment of pediatric asthma.[Methods]A total of 40 children with asthma admitted to the First Affiliated Hospital of Heilongjiang University of Chinese Medicine from July 2017 to September 2018 were selected as the research subjects,and divided into control group and observation group according to random classification method,with 20 cases in each group.Among which the control group was given routine symptomatic treatment,the observation group was treated with Acupoint Application of Jiezi Fangxiao Ointment combined with Pingchuan Prescription on the basis of the control group.After 4 weeks of continuous treatment,the clinical efficacy was observed and the changes of traditional Chinese medicine symptom scores,forced expiratory volume in the first second(FEV1),maximum expiratory flow(PEF),forced vital capacity(FVC),peripheral blood eosinophils(EOS)and interleukin-17(IL-17)were compared between the two groups before and after treatment.[Results]After treatment,the traditional Chinese medicine(TCM)scores such as asthma,cough,expectoration,chest tightness,dyspnea and wheezing sound of lungs in the two groups were significantly improved,and the improvement in the observation group was superior to that in the control group.The difference was statistically significant(P<0.05).FEV1,PEF and FVC in the two groups were significantly improved after treatment,and the improvement in the observation group was superior to that in the control group.The difference was statistically significant(P<0.05);after treatment,the levels of EOS and IL-17 decreased in both groups,and the improvement in the observation group was superior to that in the control group,the difference was statistically significant(P<0.05).[Conclusions]The clinical effects of Acupoint Application of Jiezi Fangxiao Ointment combined with Pingchuan Prescription in the treatment of infantile asthma were obvious,which can significantly improve the TCM symptoms scores,pulmonary function and immune function of patients.展开更多
目的观察固本防哮饮对信号转导和转录因子1(STAT1)及哮喘易感基因血清类黏蛋白1样蛋白3(ORMDL3)的调控作用,分析其防治哮喘机制。方法利用白介素-4诱导人支气管上皮细胞建立哮喘细胞模型,固本防哮饮含药血清对其进行干预,采用实时荧光定...目的观察固本防哮饮对信号转导和转录因子1(STAT1)及哮喘易感基因血清类黏蛋白1样蛋白3(ORMDL3)的调控作用,分析其防治哮喘机制。方法利用白介素-4诱导人支气管上皮细胞建立哮喘细胞模型,固本防哮饮含药血清对其进行干预,采用实时荧光定量-PCR(Real Time PCR)、免疫印迹法(Western blotting,WB)、免疫荧光法(IF)分别从基因、蛋白水平检测细胞中ORMDL3和STAT1的表达及亚细胞定位。结果与正常组比较,模型组ORMDL3、STAT1 m RNA含量升高,但差异无统计学意义(P>0.05),固本防哮饮可降低模型组细胞ORMDL3、STAT1 m RNA水平,差异无统计学意义(P>0.05);与正常组比较,模型组ORMDL3、STAT1蛋白表达显著增加(P<0.05或P<0.01),与模型组相比,固本防哮饮组ORMDL3及STAT1蛋白表达均显著(P<0.05)。结论固本防哮饮对STAT1及ORMDL3的表达有一定的调控作用,可能为固本防哮饮防治哮喘的内在机制。展开更多
基金General Project of Natural Science Foundation of Heilongjiang Province(H2018052).
文摘[Objectives]To study the clinical effect of Acupoint Application of Jiezi Fangxiao Ointment combined with Pingchuan Prescription in the treatment of pediatric asthma.[Methods]A total of 40 children with asthma admitted to the First Affiliated Hospital of Heilongjiang University of Chinese Medicine from July 2017 to September 2018 were selected as the research subjects,and divided into control group and observation group according to random classification method,with 20 cases in each group.Among which the control group was given routine symptomatic treatment,the observation group was treated with Acupoint Application of Jiezi Fangxiao Ointment combined with Pingchuan Prescription on the basis of the control group.After 4 weeks of continuous treatment,the clinical efficacy was observed and the changes of traditional Chinese medicine symptom scores,forced expiratory volume in the first second(FEV1),maximum expiratory flow(PEF),forced vital capacity(FVC),peripheral blood eosinophils(EOS)and interleukin-17(IL-17)were compared between the two groups before and after treatment.[Results]After treatment,the traditional Chinese medicine(TCM)scores such as asthma,cough,expectoration,chest tightness,dyspnea and wheezing sound of lungs in the two groups were significantly improved,and the improvement in the observation group was superior to that in the control group.The difference was statistically significant(P<0.05).FEV1,PEF and FVC in the two groups were significantly improved after treatment,and the improvement in the observation group was superior to that in the control group.The difference was statistically significant(P<0.05);after treatment,the levels of EOS and IL-17 decreased in both groups,and the improvement in the observation group was superior to that in the control group,the difference was statistically significant(P<0.05).[Conclusions]The clinical effects of Acupoint Application of Jiezi Fangxiao Ointment combined with Pingchuan Prescription in the treatment of infantile asthma were obvious,which can significantly improve the TCM symptoms scores,pulmonary function and immune function of patients.
文摘目的观察固本防哮饮对信号转导和转录因子1(STAT1)及哮喘易感基因血清类黏蛋白1样蛋白3(ORMDL3)的调控作用,分析其防治哮喘机制。方法利用白介素-4诱导人支气管上皮细胞建立哮喘细胞模型,固本防哮饮含药血清对其进行干预,采用实时荧光定量-PCR(Real Time PCR)、免疫印迹法(Western blotting,WB)、免疫荧光法(IF)分别从基因、蛋白水平检测细胞中ORMDL3和STAT1的表达及亚细胞定位。结果与正常组比较,模型组ORMDL3、STAT1 m RNA含量升高,但差异无统计学意义(P>0.05),固本防哮饮可降低模型组细胞ORMDL3、STAT1 m RNA水平,差异无统计学意义(P>0.05);与正常组比较,模型组ORMDL3、STAT1蛋白表达显著增加(P<0.05或P<0.01),与模型组相比,固本防哮饮组ORMDL3及STAT1蛋白表达均显著(P<0.05)。结论固本防哮饮对STAT1及ORMDL3的表达有一定的调控作用,可能为固本防哮饮防治哮喘的内在机制。
