AIM： To determine the role of Fas/Fas ligand （FasL） in the immune escape of colon cancer cells. METHODS： Immunohistochemistry was used to observe the expression of Fas and FasL in the tissues of colon cancer patie...AIM： To determine the role of Fas/Fas ligand （FasL） in the immune escape of colon cancer cells. METHODS： Immunohistochemistry was used to observe the expression of Fas and FasL in the tissues of colon cancer patients. In situ hybridization was used to detect the localization of FasL mRNA expression in cancer tissues. Terminal deoxynucleotide transferase-mediated dUTP nick end labeling （TUNEL） assay and CD45 staining were performed to detect the apoptosis of tumor-infiltrating lymphocytes （TILs）. Co-culture assays of colon cancer cells （SW480） and Jurkat cells （Fas-sensitive cells） were performed to observe the counterattack of colon cancer cells to lymphocytes. RESULTS： Of 53 cases of colon carcinomas, 23 cases （43.4%） expressed Fas which was significantly lower as compared to the normal colonic mucosa （73.3%, P〈0.01）, and 45 cases （84.9%） of colon carcinomas expressed FasL, whereas only two cases （3.75%） in normal mucosa expressed FasL. FasL expression in the colon cancer cells was found to be associated with increased cell death of TIEs. The apoptotic rate of TIL in the FasL-positive staining regions of tumor cells was significantly higher than that in the FasL-negative staining region （54.84±2.79% vs 25.73±1.98%, P〈0.01）. The co-culture of SW480 cells and Jurkat cells confirmed the function of FasL on the SW480 cells. The apoptotic rates of Jurkat cells were found to be related with the amount of SW480 cells. CONCLUSION： Colon cancer cells can escape the immune surveillance and killing via decreasing Fas expression, and can counterattack the immune system via increasing FasL expression. Fas/FasL can serve as potential targets for effective antitumor therapy.展开更多
目的:本研究旨在评估FAS和B7-H4在重度子痫前期(sPE)中的诊断价值及其对围产期不良心血管结局的预测价值。方法:使用GEO2R在线工具分析GSE2347219数据集。收集60例健康孕妇和60名sPE患者,并收集血清。通过RT-qPCR分析血清B7-H4和FAS的...目的:本研究旨在评估FAS和B7-H4在重度子痫前期(sPE)中的诊断价值及其对围产期不良心血管结局的预测价值。方法:使用GEO2R在线工具分析GSE2347219数据集。收集60例健康孕妇和60名sPE患者,并收集血清。通过RT-qPCR分析血清B7-H4和FAS的表达水平。通过直接ROC分析评估FAS和B7-H4分别对sPE的诊断价值和不良心血管结局的预测价值。通过二元logistics回归分析因子预测概率值,然后进行ROC分析,评估AS和B7-H4联合对sPE的诊断价值和不良心血管结局的预测价值。使用sPE血清培养心肌细胞,检测细胞中的肥大因子和纤维化因子。结果:GSE2347219数据集分析结果显示,FAS和B7-H4在sPE胎盘组织中上调。血清检测结果表明,FAS和B7-H4在sPE血清中上调。直接ROC分析结果表明,FAS和B7-H4可以区分sPE病例与正常孕妇,而且可以预测不良心血管结局。二元logistics回归分析和ROC分析结果表明,B7-H4联合FAS可以有效地区分sPE病例与正常孕妇并有效预测不良心血管结局。sPE血清培养可以升高心肌细胞中的肥大因子(ANP、BNP和β-MHC)和纤维化因子COL1A1的水平。结论:B7-H4 (VTCN1)联合FAS可以作为sPE患者的诊断因子和不良心血管结局的预测因子。Objective: The aim of this study was to evaluate the diagnostic value of FAS and B7-H4 in severe preeclampsia (sPE) and its predictive value for adverse perinatal cardiovascular outcomes. Methods: The GSE2347219 dataset was analyzed using the GEO2R online tool. Sixty healthy pregnant women and sixty patients who were sPE were collected and serum was collected. The expression levels of serum B7-H4 and FAS were analyzed by RT-qPCR. The diagnostic value of FAS and B7-H4 for sPE and the predictive value of adverse cardiovascular outcomes were assessed by direct ROC analysis. Predictive probability values were analyzed by binary logistics regression followed by ROC analysis to assess the diagnostic value and predictive value of adverse cardiovascular outcomes of combined FAS and B7-H4. Cardiomyocytes were cultured using sPE serum, and hypertrophic and fibrotic factors were detected in the cells. Results: Analysis of GSE2347219 dataset showed that FAS and B7-H4 were upregulated in sPE placental tissues. Serum assay results indicated that FAS and B7-H4 were upregulated in sPE serum. Direct ROC analysis showed that FAS and B7-H4 could distinguish sPE cases from normal pregnant women and that they could predict adverse cardiovascular outcomes. Binary logistic regression analysis and ROC analysis showed that B7-H4 in combination with FAS could effectively differentiate sPE cases from normal pregnant women and effectively predict adverse cardiovascular outcomes. sPE serum culture elevated the levels of hypertrophic factors (ANP, BNP, and β-MHC) and fibrosis factor COL1A1 in cardiomyocytes. Conclusion: B7-H4 (VTCN1) in combination with FAS can be used as a diagnostic factor and predictor of adverse cardiovascular outcomes in patients with sPE.展开更多
文摘AIM： To determine the role of Fas/Fas ligand （FasL） in the immune escape of colon cancer cells. METHODS： Immunohistochemistry was used to observe the expression of Fas and FasL in the tissues of colon cancer patients. In situ hybridization was used to detect the localization of FasL mRNA expression in cancer tissues. Terminal deoxynucleotide transferase-mediated dUTP nick end labeling （TUNEL） assay and CD45 staining were performed to detect the apoptosis of tumor-infiltrating lymphocytes （TILs）. Co-culture assays of colon cancer cells （SW480） and Jurkat cells （Fas-sensitive cells） were performed to observe the counterattack of colon cancer cells to lymphocytes. RESULTS： Of 53 cases of colon carcinomas, 23 cases （43.4%） expressed Fas which was significantly lower as compared to the normal colonic mucosa （73.3%, P〈0.01）, and 45 cases （84.9%） of colon carcinomas expressed FasL, whereas only two cases （3.75%） in normal mucosa expressed FasL. FasL expression in the colon cancer cells was found to be associated with increased cell death of TIEs. The apoptotic rate of TIL in the FasL-positive staining regions of tumor cells was significantly higher than that in the FasL-negative staining region （54.84±2.79% vs 25.73±1.98%, P〈0.01）. The co-culture of SW480 cells and Jurkat cells confirmed the function of FasL on the SW480 cells. The apoptotic rates of Jurkat cells were found to be related with the amount of SW480 cells. CONCLUSION： Colon cancer cells can escape the immune surveillance and killing via decreasing Fas expression, and can counterattack the immune system via increasing FasL expression. Fas/FasL can serve as potential targets for effective antitumor therapy.
文摘目的:本研究旨在评估FAS和B7-H4在重度子痫前期(sPE)中的诊断价值及其对围产期不良心血管结局的预测价值。方法:使用GEO2R在线工具分析GSE2347219数据集。收集60例健康孕妇和60名sPE患者,并收集血清。通过RT-qPCR分析血清B7-H4和FAS的表达水平。通过直接ROC分析评估FAS和B7-H4分别对sPE的诊断价值和不良心血管结局的预测价值。通过二元logistics回归分析因子预测概率值,然后进行ROC分析,评估AS和B7-H4联合对sPE的诊断价值和不良心血管结局的预测价值。使用sPE血清培养心肌细胞,检测细胞中的肥大因子和纤维化因子。结果:GSE2347219数据集分析结果显示,FAS和B7-H4在sPE胎盘组织中上调。血清检测结果表明,FAS和B7-H4在sPE血清中上调。直接ROC分析结果表明,FAS和B7-H4可以区分sPE病例与正常孕妇,而且可以预测不良心血管结局。二元logistics回归分析和ROC分析结果表明,B7-H4联合FAS可以有效地区分sPE病例与正常孕妇并有效预测不良心血管结局。sPE血清培养可以升高心肌细胞中的肥大因子(ANP、BNP和β-MHC)和纤维化因子COL1A1的水平。结论:B7-H4 (VTCN1)联合FAS可以作为sPE患者的诊断因子和不良心血管结局的预测因子。Objective: The aim of this study was to evaluate the diagnostic value of FAS and B7-H4 in severe preeclampsia (sPE) and its predictive value for adverse perinatal cardiovascular outcomes. Methods: The GSE2347219 dataset was analyzed using the GEO2R online tool. Sixty healthy pregnant women and sixty patients who were sPE were collected and serum was collected. The expression levels of serum B7-H4 and FAS were analyzed by RT-qPCR. The diagnostic value of FAS and B7-H4 for sPE and the predictive value of adverse cardiovascular outcomes were assessed by direct ROC analysis. Predictive probability values were analyzed by binary logistics regression followed by ROC analysis to assess the diagnostic value and predictive value of adverse cardiovascular outcomes of combined FAS and B7-H4. Cardiomyocytes were cultured using sPE serum, and hypertrophic and fibrotic factors were detected in the cells. Results: Analysis of GSE2347219 dataset showed that FAS and B7-H4 were upregulated in sPE placental tissues. Serum assay results indicated that FAS and B7-H4 were upregulated in sPE serum. Direct ROC analysis showed that FAS and B7-H4 could distinguish sPE cases from normal pregnant women and that they could predict adverse cardiovascular outcomes. Binary logistic regression analysis and ROC analysis showed that B7-H4 in combination with FAS could effectively differentiate sPE cases from normal pregnant women and effectively predict adverse cardiovascular outcomes. sPE serum culture elevated the levels of hypertrophic factors (ANP, BNP, and β-MHC) and fibrosis factor COL1A1 in cardiomyocytes. Conclusion: B7-H4 (VTCN1) in combination with FAS can be used as a diagnostic factor and predictor of adverse cardiovascular outcomes in patients with sPE.
