Background Grape and winery by-products have nutritional values for cattle and also contain functional compounds like phenols,which not only bind to protein but can also directly affect microbiota and their function i...Background Grape and winery by-products have nutritional values for cattle and also contain functional compounds like phenols,which not only bind to protein but can also directly affect microbiota and their function in the rumen.We characterized the nutritional and functional effects of grape seed meal and grape pomace as well as an effective dosage of grape phenols on ruminal microbiota and fermentation characteristics using a rumen simulation technique.Results Six diets(each n=8)were compared including a control diet(CON,no by-product),a positive control diet(EXT,CON+3.7%grape seed extract on a dry matter(DM)basis),two diets with grape seed meal at 5%(GS-low)and 10%(GS-high),and two diets with grape pomace:at 10%(GP-low)and 20%(GP-high),on a DM basis.The inclusion of the by-product supplied total phenols at 3.4%,0.7%,1.4%,1.3%,and 2.7%of diet DM for EXT,GS-low,GS-high,GP-low,and GP-high,respectively.Diets were tested in four experimental runs.All treatments decreased ammonia concentrations and the disappearances of DM and OM(P<0.05)compared to CON.EXT and GP-high lowered butyrate and odd-and branch-chain short-chain fatty acids while increased acetate compared to CON(P<0.05).Treatments did not affect methane formation.EXT decreased the abundance of many bacterial genera including those belonging to the core microbiota.GP-high and EXT consistently decreased Olsenella and Anaerotipes while increased Ruminobacter abundances.Conclusion The data suggest that the inclusion of winery by-products or grape seed extract could be an option for reducing excessive ammonia production.Exposure to grape phenols at a high dosage in an extract form can alter the rumen microbial community.This,however,does not necessarily alter the effect of grape phenols on the microbial community function compared to feeding high levels of winery by-products.This suggests the dominant role of dosage over the form or source of the grape phenols in affecting ruminal microbial activity.In conclusion,supplementing grape phenols at about 3%of diet DM is an effective dosage tolerable to ruminal microbiota.展开更多
There is an immediate need to identify alternative sources of high-nutrient feedstocks for domestic livestock productionand poultry,not only to support growing food demands but also to produce microalgae-source functi...There is an immediate need to identify alternative sources of high-nutrient feedstocks for domestic livestock productionand poultry,not only to support growing food demands but also to produce microalgae-source functional foods withmultiple health benefits.Various species of microalgae and cyanobacteria are used to supplement existing feedstocks.Inthis review,microalgae have been defined as a potential feedstock for domestic animals due to their abundance ofproteins,carbohydrates,lipids,minerals,vitamins,and other high-value products.Additionally,the positive physiologicaleffects on products of animals fed with microalgal biomass have been compiled and recommendations are listed toenhance the assimilation of biomolecules in ruminant and nonruminant animals,which possess differing digestivesystems.Furthermore,the role of microalgae as prebiotics is also discussed.With regards to large scale cultivation ofmicroalgae for use as feed,many economic trade-offs must be considered such as the selection of strains with desirednutritional properties,cultivation systems,and steps for downstream processing.These factors are highlighted withfurther investigations needed to reduce the overall costs of cultivation.Finally,this review outlines the pros and cons ofutilizing microalgae as a supplementary feedstock for poultry and cattle,existing cultivation strategies,and theeconomics of large-scale microalgal production.展开更多
基金provided by the University of Veterinary Medicine Vienna (Vetmeduni Vienna)funded by Hochschuljubilaumsstiftung der Stadt Wien (project H-288490/2019)
文摘Background Grape and winery by-products have nutritional values for cattle and also contain functional compounds like phenols,which not only bind to protein but can also directly affect microbiota and their function in the rumen.We characterized the nutritional and functional effects of grape seed meal and grape pomace as well as an effective dosage of grape phenols on ruminal microbiota and fermentation characteristics using a rumen simulation technique.Results Six diets(each n=8)were compared including a control diet(CON,no by-product),a positive control diet(EXT,CON+3.7%grape seed extract on a dry matter(DM)basis),two diets with grape seed meal at 5%(GS-low)and 10%(GS-high),and two diets with grape pomace:at 10%(GP-low)and 20%(GP-high),on a DM basis.The inclusion of the by-product supplied total phenols at 3.4%,0.7%,1.4%,1.3%,and 2.7%of diet DM for EXT,GS-low,GS-high,GP-low,and GP-high,respectively.Diets were tested in four experimental runs.All treatments decreased ammonia concentrations and the disappearances of DM and OM(P<0.05)compared to CON.EXT and GP-high lowered butyrate and odd-and branch-chain short-chain fatty acids while increased acetate compared to CON(P<0.05).Treatments did not affect methane formation.EXT decreased the abundance of many bacterial genera including those belonging to the core microbiota.GP-high and EXT consistently decreased Olsenella and Anaerotipes while increased Ruminobacter abundances.Conclusion The data suggest that the inclusion of winery by-products or grape seed extract could be an option for reducing excessive ammonia production.Exposure to grape phenols at a high dosage in an extract form can alter the rumen microbial community.This,however,does not necessarily alter the effect of grape phenols on the microbial community function compared to feeding high levels of winery by-products.This suggests the dominant role of dosage over the form or source of the grape phenols in affecting ruminal microbial activity.In conclusion,supplementing grape phenols at about 3%of diet DM is an effective dosage tolerable to ruminal microbiota.
基金This report was made possible the NPRP award[NPRP8–1087–1-207]from the Qatar National Research Fund,a member of The Qatar Foundation.The statements made herein are solely the responsibility of the authors.Open Access funding provided by the Qatar National Library.
文摘There is an immediate need to identify alternative sources of high-nutrient feedstocks for domestic livestock productionand poultry,not only to support growing food demands but also to produce microalgae-source functional foods withmultiple health benefits.Various species of microalgae and cyanobacteria are used to supplement existing feedstocks.Inthis review,microalgae have been defined as a potential feedstock for domestic animals due to their abundance ofproteins,carbohydrates,lipids,minerals,vitamins,and other high-value products.Additionally,the positive physiologicaleffects on products of animals fed with microalgal biomass have been compiled and recommendations are listed toenhance the assimilation of biomolecules in ruminant and nonruminant animals,which possess differing digestivesystems.Furthermore,the role of microalgae as prebiotics is also discussed.With regards to large scale cultivation ofmicroalgae for use as feed,many economic trade-offs must be considered such as the selection of strains with desirednutritional properties,cultivation systems,and steps for downstream processing.These factors are highlighted withfurther investigations needed to reduce the overall costs of cultivation.Finally,this review outlines the pros and cons ofutilizing microalgae as a supplementary feedstock for poultry and cattle,existing cultivation strategies,and theeconomics of large-scale microalgal production.
