Objective: To establish a DNA detection platform based on a tapered optical fiber to detect Leptospira DNA by targeting the leptospiral secY gene.Methods: The biosensor works on the principle of light propagating in t...Objective: To establish a DNA detection platform based on a tapered optical fiber to detect Leptospira DNA by targeting the leptospiral secY gene.Methods: The biosensor works on the principle of light propagating in the special geometry of the optical fiber tapered from a waist diameter of 125 to 12 μm. The fiber surface was functionalized through a cascade of chemical treatments and the immobilization of a DNA capture probe targeting the secY gene. The presence of the target DNA was determined from the wavelength shift in the optical transmission spectrum.Results: The biosensor demonstrated good sensitivity, detecting Leptospira DNA at 0.001 ng/μL, and was selective for Leptospira DNA without cross-reactivity with non-leptospiral microorganisms. The biosensor specifically detected DNA that was specifically amplified through the loop-mediated isothermal amplification approach.Conclusions: These findings warrant the potential of this platform to be developed as a novel alternative approach to diagnose leptospirosis.展开更多
A temperature sensor is demonstrated and fabricated by coating the single-mode tapered optical fiber with temperature-sensitive silicone rubber. It works on the change of the evanescent fields in the tapered optical f...A temperature sensor is demonstrated and fabricated by coating the single-mode tapered optical fiber with temperature-sensitive silicone rubber. It works on the change of the evanescent fields in the tapered optical fiber. Small changes in the refractive index of coating film greatly influence the power of evanescent fields, which modulate the transmission optical power in the waist region. The range of temperature measured is from -20℃ to 70 ℃. The results show that the temperature sensor has high temperature sensitivity （0.012 mW/℃） and good repeatability.展开更多
We demonstrate a stable Yb：fiber frequency comb with supercontinuum generation by using a specially designed tapered single-mode fiber, in which a spectrum spanning from 500 nm to 1500 nm is produced. The carrier-env...We demonstrate a stable Yb：fiber frequency comb with supercontinuum generation by using a specially designed tapered single-mode fiber, in which a spectrum spanning from 500 nm to 1500 nm is produced. The carrier-envelope offset signal of the Yb：fiber comb is measured with a signal-to-noise ratio of more than 40 dB and a linewidth narrower than 120 kHz. The repetition rate and carrier-envelope offset signals are simultaneously phase locked to a microwave reference frequency.展开更多
In order to detect the refractive index of liquid with high precision,based on modular interference,Mach-Zehnder optical fiber refractive rate sensor was studied.Sensor structure is composed of ordinary single-mode fi...In order to detect the refractive index of liquid with high precision,based on modular interference,Mach-Zehnder optical fiber refractive rate sensor was studied.Sensor structure is composed of ordinary single-mode fiber and multimode fiber,according to the singlemode multimode singlemode sequence to fuse together,and the fused optical fiber is used to process the taper.As a result,the diameter of the sensing head is about 10μm.Experimental results show that,as liquid refractive index increases range from 1.33 to 1.35,the loss peak of the transmission spectrum will shift to long wave direction.展开更多
A brief review on biconical tapered fiber sensors for biosensing applications is presented. A variety of configurations and formats of this sensor have been devised for label free biosensing based on measuring small r...A brief review on biconical tapered fiber sensors for biosensing applications is presented. A variety of configurations and formats of this sensor have been devised for label free biosensing based on measuring small refractive index changes. The biconical nonadiabatic tapered optical fiber offers a number of favorable properties for optical sensing, which have been exploited in several biosensing applications, including cell, protein, and DNA sensors. The types of these sensors present a low-cost fiber biosensor featuring a miniature sensing probe, label-free direct detection, and high sensitivity.展开更多
A rapid and high-performance sensor for lard adulterant in edible oil was developed using the tapered plastic optical fiber(POF)coated with graphene and multi-walled carbon nanotubes.The coating material was deposited...A rapid and high-performance sensor for lard adulterant in edible oil was developed using the tapered plastic optical fiber(POF)coated with graphene and multi-walled carbon nanotubes.The coating material was deposited onto a tapered POF with a taper waist diameter and a taper length of 0.45 mm and 1 cm,respectively.The addition of the coating material was used to increase the sensitivity and selectivity coefficient of the tapered POF toward the lard substance.The sensing mechanism is based on a simultaneous interaction of lard substance and an evanescent wave of tapered POF with the coating layers.The results showed that graphene coating on the tapered POF increased the selectivity coefficient of the tapered POF towards lard substance from 33.54 to 324.19,and it gave a sensitivity of 0.427 dBm/%.In comparison,multi-walled carbon nanotubes coating increased the selectivity coefficient to 71.65 and increased its sensitivity to 1.189 dBm/%.Thus,the proposed configuration of the tapered POF with the coating material offered a simple configuration for a rapid,high sensitivity and selectivity detection of lard adulterant in edible oils.展开更多
An evanescent wave absorption （EWA） sensor based tapered plastic optical fiber （TPOF） coated with monolayer graphene film for ethanol molecules detection is demonstrated in this study. The continuous and monolayer...An evanescent wave absorption （EWA） sensor based tapered plastic optical fiber （TPOF） coated with monolayer graphene film for ethanol molecules detection is demonstrated in this study. The continuous and monolayer graphene films were grown on a Cu foil by using chemical vapor deposition （CVD） technology. Polymethyl methacrylate （PMMA） was used as the carrier to support the transfer of graphene from Cu foil to the skinless tapered optical fiber core. The accuracy of the TPOF sensor with graphene （G-TPOF sensor） is much higher than that without gra- phene （TPOF sensor）, which can be attributed to the molecules enriched on the surface of graphene. The absorbance （A） and the concentrations of ethanol solution show an excellent proportional relationship in a range of 0--100%. The dynamic response of the G-TPOF sensor has shown strong reversibility, repeatability and stability at room temperature. The response time and recovery time of the G-TPOF sensor for different concentrations are all less than 30 s. Beyond that, we selected the Chinese liquor as the analyte, and the results are consistent with the concen- tration-list obtained in the experiment.展开更多
基金funded by Universiti Putra Malaysia through the Geran Inisiatif Putra Siswazah (GP-IPS/2019/9678200)。
文摘Objective: To establish a DNA detection platform based on a tapered optical fiber to detect Leptospira DNA by targeting the leptospiral secY gene.Methods: The biosensor works on the principle of light propagating in the special geometry of the optical fiber tapered from a waist diameter of 125 to 12 μm. The fiber surface was functionalized through a cascade of chemical treatments and the immobilization of a DNA capture probe targeting the secY gene. The presence of the target DNA was determined from the wavelength shift in the optical transmission spectrum.Results: The biosensor demonstrated good sensitivity, detecting Leptospira DNA at 0.001 ng/μL, and was selective for Leptospira DNA without cross-reactivity with non-leptospiral microorganisms. The biosensor specifically detected DNA that was specifically amplified through the loop-mediated isothermal amplification approach.Conclusions: These findings warrant the potential of this platform to be developed as a novel alternative approach to diagnose leptospirosis.
基金Project supported by the Shanghai Leading Academic Discipline Project (Grant No.S30108)the Universities Foster Innovation Foundation Projects for Major Projects in Ministry of Education (Grant No.708041)
文摘A temperature sensor is demonstrated and fabricated by coating the single-mode tapered optical fiber with temperature-sensitive silicone rubber. It works on the change of the evanescent fields in the tapered optical fiber. Small changes in the refractive index of coating film greatly influence the power of evanescent fields, which modulate the transmission optical power in the waist region. The range of temperature measured is from -20℃ to 70 ℃. The results show that the temperature sensor has high temperature sensitivity （0.012 mW/℃） and good repeatability.
基金supported by the National Basic Research Program of China(973 Program)(Grant No.2012CB821304)the National Natural Science Foundation of China(Grant No.61378040)
文摘We demonstrate a stable Yb：fiber frequency comb with supercontinuum generation by using a specially designed tapered single-mode fiber, in which a spectrum spanning from 500 nm to 1500 nm is produced. The carrier-envelope offset signal of the Yb：fiber comb is measured with a signal-to-noise ratio of more than 40 dB and a linewidth narrower than 120 kHz. The repetition rate and carrier-envelope offset signals are simultaneously phase locked to a microwave reference frequency.
基金National Natural Science Foundation of China(No.61405127)Shanxi Province Science Foundation for Youths(No.2014021023-1)+1 种基金Scientific and Technologial Innovation Programs of Higher Education Institutions in Shanxi ProvinceProgram for Top Young Academic Leaders of Higher Learning Institutions in Shanxi Province
文摘In order to detect the refractive index of liquid with high precision,based on modular interference,Mach-Zehnder optical fiber refractive rate sensor was studied.Sensor structure is composed of ordinary single-mode fiber and multimode fiber,according to the singlemode multimode singlemode sequence to fuse together,and the fused optical fiber is used to process the taper.As a result,the diameter of the sensing head is about 10μm.Experimental results show that,as liquid refractive index increases range from 1.33 to 1.35,the loss peak of the transmission spectrum will shift to long wave direction.
文摘A brief review on biconical tapered fiber sensors for biosensing applications is presented. A variety of configurations and formats of this sensor have been devised for label free biosensing based on measuring small refractive index changes. The biconical nonadiabatic tapered optical fiber offers a number of favorable properties for optical sensing, which have been exploited in several biosensing applications, including cell, protein, and DNA sensors. The types of these sensors present a low-cost fiber biosensor featuring a miniature sensing probe, label-free direct detection, and high sensitivity.
基金The research has been funded by the Indonesian Government,KEMENRISTEKDIKTI(Ministry of Research,Technology,and Higher Education of the Republic of Indonesia)for supporting this work under project scheme University’s Fundamental Research Program(Grant No.918/PKS/ITS/2021).
文摘A rapid and high-performance sensor for lard adulterant in edible oil was developed using the tapered plastic optical fiber(POF)coated with graphene and multi-walled carbon nanotubes.The coating material was deposited onto a tapered POF with a taper waist diameter and a taper length of 0.45 mm and 1 cm,respectively.The addition of the coating material was used to increase the sensitivity and selectivity coefficient of the tapered POF toward the lard substance.The sensing mechanism is based on a simultaneous interaction of lard substance and an evanescent wave of tapered POF with the coating layers.The results showed that graphene coating on the tapered POF increased the selectivity coefficient of the tapered POF towards lard substance from 33.54 to 324.19,and it gave a sensitivity of 0.427 dBm/%.In comparison,multi-walled carbon nanotubes coating increased the selectivity coefficient to 71.65 and increased its sensitivity to 1.189 dBm/%.Thus,the proposed configuration of the tapered POF with the coating material offered a simple configuration for a rapid,high sensitivity and selectivity detection of lard adulterant in edible oils.
基金The authors are grateful for financial support from the National Natural Science Foundation of China （Nos. 61205174, 61401258, 11504209, and 11404195）, Excellent Young Scholars Research Fund of Shandong Normal University, and Shandong Province Natural Science Foundation （No. ZR2013EMM009）.
文摘An evanescent wave absorption （EWA） sensor based tapered plastic optical fiber （TPOF） coated with monolayer graphene film for ethanol molecules detection is demonstrated in this study. The continuous and monolayer graphene films were grown on a Cu foil by using chemical vapor deposition （CVD） technology. Polymethyl methacrylate （PMMA） was used as the carrier to support the transfer of graphene from Cu foil to the skinless tapered optical fiber core. The accuracy of the TPOF sensor with graphene （G-TPOF sensor） is much higher than that without gra- phene （TPOF sensor）, which can be attributed to the molecules enriched on the surface of graphene. The absorbance （A） and the concentrations of ethanol solution show an excellent proportional relationship in a range of 0--100%. The dynamic response of the G-TPOF sensor has shown strong reversibility, repeatability and stability at room temperature. The response time and recovery time of the G-TPOF sensor for different concentrations are all less than 30 s. Beyond that, we selected the Chinese liquor as the analyte, and the results are consistent with the concen- tration-list obtained in the experiment.
