Fish populations have declined in many estuarine and freshwater ecosystems in part due to the loss of habitat in recent decades.Reconstructing lost habitat for larvae fish is a potential method for recovering larvae f...Fish populations have declined in many estuarine and freshwater ecosystems in part due to the loss of habitat in recent decades.Reconstructing lost habitat for larvae fish is a potential method for recovering larvae fish populations.Three-dimensional artificial floating wetlands(AFWs)on which Phragmites australis was planted were experimentally deployed to recover the lost habitat in the Changjiang(Yangtze)River estuary from May to July 2018.The AFW area was characterized by slow velocity,high transparency,low dissolved oxygen,and relatively constant water temperature.The total individuals of larvae fish in the AFW area(12122 in total)was higher than that in the non-AFW area(1250 in total),and the densities of most larvae fish species were higher in the AFW habitat than in the non-AFW area.The distributions of larvae fish species were positively influenced by habitat type because they were strongly related to the negative part of the first axis of the redundancy analysis,and Cyprinus carpio and Cyprinus auratus were inclined to habitat in the slow velocity and high transparency AFW habitat area.These results indicate that larvae fish species are inclined to inhabit the AFW habitat.The use of three-dimensional P.australis AFWs would be a potential method for enhancing the habitat of larvae fish in the degraded habitats along the estuary.展开更多
In this study,we investigated the transcriptional spatio-temporal dynamics of the taste 1 receptor(T1R)gene family repertoire in seabream(Sparus aurata[sa]),during larval ontogeny and in adult tissues.In early larval ...In this study,we investigated the transcriptional spatio-temporal dynamics of the taste 1 receptor(T1R)gene family repertoire in seabream(Sparus aurata[sa]),during larval ontogeny and in adult tissues.In early larval development,sa T1R expression arises heterochronously,i.e.the extraoral taste-related perception in the gastrointestinal tract(GIT)anticipates first exogenous feeding(at 9 days post hatching[dph]),followed by the buccal/intraoral perception from 14 dph onwards,supporting the hypothesis that the early onset of the molecular machinery underlying sa T1R expression in the GIT is not induced by food but rather genetically hardwired.During adulthood,we characterized the expression patterns of sa T1R within specific tissues(n=4)distributed in oropharingeal,GIT and brain regions substantiating their functional versatility as chemosensory signaling players to a variety of biological functions beyond oral taste sensation.Further,we provided for the first time direct evidences in fish for m RNA coexpression of a subset of sa T1R genes(mostly sa T1R3,i.e.the common subunit of the heterodimeric T1R complexes for the detection of“sweet”and“umami”substances),with the selected gut peptides ghrelin(ghr),cholecystokinin(cck),hormone peptide yy(pyy)and proglucagon(pg).Each peptide defines the enteroendocrine cells(ECCs)identity,and establishes on morphological basis,a direct link for T1R chemosensing in the regulation of fish digestive processes.Finally,we analyzed the spatial gene expression patterns of 2 taste signaling components functionally homologous to the mammalian G(i)a subunit gustducin,namely sa G(i)a1 and sa G(i)a2,and demonstrated their co-localization with the sa T1R3in EECs,thus validating their direct involvement in taste-like transduction mechanisms of the fish GIT.In conclusion,data provide new insights in the evolutionary conservation of gut sensing in fish suggesting a conserved role for nutrient sensors modulating entero-endocrine secretion.展开更多
基金Supported by the National Key R&D Program of China(Nos.2019YFD0901202,2018YFD0900905)the China Postdoctoral Science Foundation(No.2018M632887)。
文摘Fish populations have declined in many estuarine and freshwater ecosystems in part due to the loss of habitat in recent decades.Reconstructing lost habitat for larvae fish is a potential method for recovering larvae fish populations.Three-dimensional artificial floating wetlands(AFWs)on which Phragmites australis was planted were experimentally deployed to recover the lost habitat in the Changjiang(Yangtze)River estuary from May to July 2018.The AFW area was characterized by slow velocity,high transparency,low dissolved oxygen,and relatively constant water temperature.The total individuals of larvae fish in the AFW area(12122 in total)was higher than that in the non-AFW area(1250 in total),and the densities of most larvae fish species were higher in the AFW habitat than in the non-AFW area.The distributions of larvae fish species were positively influenced by habitat type because they were strongly related to the negative part of the first axis of the redundancy analysis,and Cyprinus carpio and Cyprinus auratus were inclined to habitat in the slow velocity and high transparency AFW habitat area.These results indicate that larvae fish species are inclined to inhabit the AFW habitat.The use of three-dimensional P.australis AFWs would be a potential method for enhancing the habitat of larvae fish in the degraded habitats along the estuary.
基金covered by the National Research Agency(AEI,Spain)(grant number:PID2019-103969RB-C33)to Jos e M.Cerd a-Reverter。
文摘In this study,we investigated the transcriptional spatio-temporal dynamics of the taste 1 receptor(T1R)gene family repertoire in seabream(Sparus aurata[sa]),during larval ontogeny and in adult tissues.In early larval development,sa T1R expression arises heterochronously,i.e.the extraoral taste-related perception in the gastrointestinal tract(GIT)anticipates first exogenous feeding(at 9 days post hatching[dph]),followed by the buccal/intraoral perception from 14 dph onwards,supporting the hypothesis that the early onset of the molecular machinery underlying sa T1R expression in the GIT is not induced by food but rather genetically hardwired.During adulthood,we characterized the expression patterns of sa T1R within specific tissues(n=4)distributed in oropharingeal,GIT and brain regions substantiating their functional versatility as chemosensory signaling players to a variety of biological functions beyond oral taste sensation.Further,we provided for the first time direct evidences in fish for m RNA coexpression of a subset of sa T1R genes(mostly sa T1R3,i.e.the common subunit of the heterodimeric T1R complexes for the detection of“sweet”and“umami”substances),with the selected gut peptides ghrelin(ghr),cholecystokinin(cck),hormone peptide yy(pyy)and proglucagon(pg).Each peptide defines the enteroendocrine cells(ECCs)identity,and establishes on morphological basis,a direct link for T1R chemosensing in the regulation of fish digestive processes.Finally,we analyzed the spatial gene expression patterns of 2 taste signaling components functionally homologous to the mammalian G(i)a subunit gustducin,namely sa G(i)a1 and sa G(i)a2,and demonstrated their co-localization with the sa T1R3in EECs,thus validating their direct involvement in taste-like transduction mechanisms of the fish GIT.In conclusion,data provide new insights in the evolutionary conservation of gut sensing in fish suggesting a conserved role for nutrient sensors modulating entero-endocrine secretion.
