Objective To analyze the etiology of food poisoning,provide data support for food poisoning treatment,and protect people’s health.Methods RT-PCR was used to screen suspicious food and vomit.An enzyme-linked immunoflu...Objective To analyze the etiology of food poisoning,provide data support for food poisoning treatment,and protect people’s health.Methods RT-PCR was used to screen suspicious food and vomit.An enzyme-linked immunofluorescence assay(ELFA)was used to detect Staphylococcus aureus enterotoxin.RT-PCR was used to detect enterotoxin genotypes.An automatic drug sensitivity reading system was used for drug resistance analysis and mass spectrometry identification.Results Staphylococcus aureus were isolated from 5 leftovers and 2 vomitus samples.The enterotoxin types of 7 strains were sed and seen;the drug sensitivity test showed that 7 strains were resistant to penicillin,and 6 strains were inducible clindamycin resistant.Conclusion This is a food poisoning event caused by Staphylococcus aureus,which produces sed and sees enterotoxins.The isolated strains have different degrees of drug resistance.展开更多
选注者言:读了这篇文章,也许我们对“生猛海鲜”这四个字会产生别样的理解。海鲜之害人,可谓“生猛”也！美国是一个环境保护较好,医疗水平较高的国度,然而,下面这个事实确实让读者乍舌: The group noted that most people who become i...选注者言:读了这篇文章,也许我们对“生猛海鲜”这四个字会产生别样的理解。海鲜之害人,可谓“生猛”也！美国是一个环境保护较好,医疗水平较高的国度,然而,下面这个事实确实让读者乍舌: The group noted that most people who become ill from food poisoning or whodie are not affected in outbreaks but in single-case incdents. It said 75 million casesof food poisoning occur in the United States each year and 5,000 people die from it.本文的另一个新信息是:蛋品也是一个不可忽视的“病源”！展开更多
Staphylococcal superantigen(SAg)toxins are the most notable virulence factors associated with Staphylococcus aureus,which is a pathogen associated with serious community and hospital acquired infections in humans and ...Staphylococcal superantigen(SAg)toxins are the most notable virulence factors associated with Staphylococcus aureus,which is a pathogen associated with serious community and hospital acquired infections in humans and various diseases in animals.Recently,SAg toxins have become a superfamily with 29 types,including staphylococcal enterotoxins(SEs)with emetic activity,SE-like toxins(SEIs)that do not induce emesis in primate models or have yet not been tested,and toxic shock syndrome toxin-1(TSST-1).SEs and SEIs can be subdivided into classical types(SEA to SEE)and novel types(SEG to SEIY,SE01,SE02,SEI26 and SEI27).The genes of SAg toxins are located in diverse accessory genetic elements and share certain structural and biological properties.SAg toxins are heat-stable proteins that exhibit pyrogenicity,superantigenicity and capacity to induce lethal hypersensitivity to endotoxin in humans and animals.They have multiple pathogenicities that can interfere with normal immune function of host,increase the chances of survival and transmission of pathogenic baaeria in host,consequently contribute to the occurrence and development of various infeaions,persistent infeaions or food poisoning.This review focuses on the following aspeas of SAg toxins:(1)superfamily members of classic and novelty discovered staphylococcal SAgs;⑵diversity of gene locations and molecular structural characteristics;(3)biological characteristics and activities;(4)multi-pathogenicity of SAgs in animal and human diseases,including bovine mastitis,swine sepsis,abscesses and skin edema in pig,arthritis and septicemia in poultry,and nosocomial infections and food-borne diseases in humans.展开更多
Objective:To investigate the chemical compositions and antimicrobial potentials of the essential oils and oleoresins obtained from fresh and dried Mentha longifolia L.Methods:Gas chromatography and gas chromatography-...Objective:To investigate the chemical compositions and antimicrobial potentials of the essential oils and oleoresins obtained from fresh and dried Mentha longifolia L.Methods:Gas chromatography and gas chromatography-mass spectrometer techniques were used to determine the profiling of the essential oils and oleoresins.In order to determine the antimicrobial efficacy of the volatile oil and oleoresins,the pathogenic fungus Aspergillus niger(1884),Aspergillus flavus(2479),Fusarium monoliforme(1893),Fusarium graminearum(2088)and Penicillium viridicatum(2007)were undertaken whereas four pathogenic bacteria Bacillus subtilis(1790),Staphylococcus aureus(3103)(Gram-positive),Escherichia coli(1672),Pseudomonas aeruginosa(1942)(Gram-negative)were selected for the present study.Food poisoned,inverted Petri plate,agar well diffusion and disk diffusion methods were employed for investigating antimicrobial potentials.Results:Piperitenone oxide,an oxygenated monoterpene,dominated the chemical compositions of essential oils and oleoresins whose compositions varied from 23.5%-87.8%.Both essential oils showed good antifungal activities against Aspergillus and Fusarium species.The antibacterial investigations revealed that Gram-positive bacteria were more sensitive to the essential oils.Conclusions:Drying the fresh herbal materials influences the chemical contents and the biological activities of the essential oils and oleoresins.Such results indicate that essential oils of Mentha longifolia L.can be possible candidates for further investigations to isolate and characterize their active principles as possible new natural preservatives.展开更多
文摘Objective To analyze the etiology of food poisoning,provide data support for food poisoning treatment,and protect people’s health.Methods RT-PCR was used to screen suspicious food and vomit.An enzyme-linked immunofluorescence assay(ELFA)was used to detect Staphylococcus aureus enterotoxin.RT-PCR was used to detect enterotoxin genotypes.An automatic drug sensitivity reading system was used for drug resistance analysis and mass spectrometry identification.Results Staphylococcus aureus were isolated from 5 leftovers and 2 vomitus samples.The enterotoxin types of 7 strains were sed and seen;the drug sensitivity test showed that 7 strains were resistant to penicillin,and 6 strains were inducible clindamycin resistant.Conclusion This is a food poisoning event caused by Staphylococcus aureus,which produces sed and sees enterotoxins.The isolated strains have different degrees of drug resistance.
文摘选注者言:读了这篇文章,也许我们对“生猛海鲜”这四个字会产生别样的理解。海鲜之害人,可谓“生猛”也！美国是一个环境保护较好,医疗水平较高的国度,然而,下面这个事实确实让读者乍舌: The group noted that most people who become ill from food poisoning or whodie are not affected in outbreaks but in single-case incdents. It said 75 million casesof food poisoning occur in the United States each year and 5,000 people die from it.本文的另一个新信息是:蛋品也是一个不可忽视的“病源”！
基金This study was supported in part by the JSPS KAKENHI Grant numbers 19590438(D.H.)21590475(D.H.)+1 种基金24590516(D.H.)16H05030(D.H.).
文摘Staphylococcal superantigen(SAg)toxins are the most notable virulence factors associated with Staphylococcus aureus,which is a pathogen associated with serious community and hospital acquired infections in humans and various diseases in animals.Recently,SAg toxins have become a superfamily with 29 types,including staphylococcal enterotoxins(SEs)with emetic activity,SE-like toxins(SEIs)that do not induce emesis in primate models or have yet not been tested,and toxic shock syndrome toxin-1(TSST-1).SEs and SEIs can be subdivided into classical types(SEA to SEE)and novel types(SEG to SEIY,SE01,SE02,SEI26 and SEI27).The genes of SAg toxins are located in diverse accessory genetic elements and share certain structural and biological properties.SAg toxins are heat-stable proteins that exhibit pyrogenicity,superantigenicity and capacity to induce lethal hypersensitivity to endotoxin in humans and animals.They have multiple pathogenicities that can interfere with normal immune function of host,increase the chances of survival and transmission of pathogenic baaeria in host,consequently contribute to the occurrence and development of various infeaions,persistent infeaions or food poisoning.This review focuses on the following aspeas of SAg toxins:(1)superfamily members of classic and novelty discovered staphylococcal SAgs;⑵diversity of gene locations and molecular structural characteristics;(3)biological characteristics and activities;(4)multi-pathogenicity of SAgs in animal and human diseases,including bovine mastitis,swine sepsis,abscesses and skin edema in pig,arthritis and septicemia in poultry,and nosocomial infections and food-borne diseases in humans.
基金Supported by the University Grants Commission(Granted No.F.17-28/2008 SA-I).
文摘Objective:To investigate the chemical compositions and antimicrobial potentials of the essential oils and oleoresins obtained from fresh and dried Mentha longifolia L.Methods:Gas chromatography and gas chromatography-mass spectrometer techniques were used to determine the profiling of the essential oils and oleoresins.In order to determine the antimicrobial efficacy of the volatile oil and oleoresins,the pathogenic fungus Aspergillus niger(1884),Aspergillus flavus(2479),Fusarium monoliforme(1893),Fusarium graminearum(2088)and Penicillium viridicatum(2007)were undertaken whereas four pathogenic bacteria Bacillus subtilis(1790),Staphylococcus aureus(3103)(Gram-positive),Escherichia coli(1672),Pseudomonas aeruginosa(1942)(Gram-negative)were selected for the present study.Food poisoned,inverted Petri plate,agar well diffusion and disk diffusion methods were employed for investigating antimicrobial potentials.Results:Piperitenone oxide,an oxygenated monoterpene,dominated the chemical compositions of essential oils and oleoresins whose compositions varied from 23.5%-87.8%.Both essential oils showed good antifungal activities against Aspergillus and Fusarium species.The antibacterial investigations revealed that Gram-positive bacteria were more sensitive to the essential oils.Conclusions:Drying the fresh herbal materials influences the chemical contents and the biological activities of the essential oils and oleoresins.Such results indicate that essential oils of Mentha longifolia L.can be possible candidates for further investigations to isolate and characterize their active principles as possible new natural preservatives.
