Food protein-induced enterocolitis syndrome(FPIES)is a non-Immunoglobulin(non-IgE)-mediated food allergy.The elimination diet is the only therapy,the culprit food will be reintroduced if tolerance is acquired.However,...Food protein-induced enterocolitis syndrome(FPIES)is a non-Immunoglobulin(non-IgE)-mediated food allergy.The elimination diet is the only therapy,the culprit food will be reintroduced if tolerance is acquired.However,it is possible that patients do not follow the recommendations given by the healthcare professional.We investigated if our advice to avoid the trigger food in patients with active FPIES and to reintroduce it in the diet in patients who achieved tolerance had been implemented.We interviewed by telephone the parents of children who were diagnosed with acute FPIES.About 23.2%of our patients disregarded our dietary recommendations:6/42(14.3%)of patients who passed a tolerance oral food challenge(OFC)did not eat the trigger food,4/22(18.2%)of patients who failed OFC ate the trigger food,and 9/18(50.0%)of patients who did not perform a tolerance OFC ate the trigger food.We have analyzed some possible influencing factors and no difference was found to be statistically significant.Our results are in line with those reported for IgE-mediated food allergies.As has already been proposed by others,we suggest reassessing food consumption in all patients after a food challenge.展开更多
目的通过高通量测序分析食物蛋白诱导性直肠结肠炎(food protein-induced proctocolitis,FPIP)患儿肠道菌群特征。方法选取2018年10月至2021年2月遵义医科大学第三附属医院门诊就诊的小于6月龄纯母乳喂养发生FPIP患儿31例纳入FPIP组,正...目的通过高通量测序分析食物蛋白诱导性直肠结肠炎(food protein-induced proctocolitis,FPIP)患儿肠道菌群特征。方法选取2018年10月至2021年2月遵义医科大学第三附属医院门诊就诊的小于6月龄纯母乳喂养发生FPIP患儿31例纳入FPIP组,正常健康婴儿31例纳入健康对照组;采集两组粪便样本提取DNA并通过PCR扩增,采用高通量测序分析粪便样品中的16S r DNA V3~V4片段并进行相关生物信息学分析。结果肠道菌群多样性分析示FPIP组菌群多样性Shannon指数低于健康对照组,但差异无统计学意义(P>0.05);菌群丰富度Chao指数高于健康对照组(P<0.01)。在门水平上,两组菌群组成均主要由厚壁菌门、放线菌门、变形菌门、拟杆菌门4个菌门构成;与健康对照组相比,FPIP组中放线菌门构成比显著降低(P<0.001),变形菌门构成比明显增加(P<0.05)。在属水平上,FPIP组主要由大肠埃希氏菌属、梭状芽孢杆菌属、肠球菌属、克雷伯氏菌属和双歧杆菌属组成;健康对照组主要由双歧杆菌属、链球菌属组成;与健康对照组相比,FPIP组中双歧杆菌属和瘤胃球菌属构成比明显降低(P<0.05),梭状芽孢杆菌属和志贺氏菌属构成比明显增加(P<0.05)。结论FPIP组与健康对照组相比,肠道菌群多样性下降,丰富度升高;在菌群组成结构上某些细菌菌属存在差异。提示在FPIP的发生发展过程中,肠道微生物群在属水平上的构成改变可能起到了重要作用。展开更多
文摘Food protein-induced enterocolitis syndrome(FPIES)is a non-Immunoglobulin(non-IgE)-mediated food allergy.The elimination diet is the only therapy,the culprit food will be reintroduced if tolerance is acquired.However,it is possible that patients do not follow the recommendations given by the healthcare professional.We investigated if our advice to avoid the trigger food in patients with active FPIES and to reintroduce it in the diet in patients who achieved tolerance had been implemented.We interviewed by telephone the parents of children who were diagnosed with acute FPIES.About 23.2%of our patients disregarded our dietary recommendations:6/42(14.3%)of patients who passed a tolerance oral food challenge(OFC)did not eat the trigger food,4/22(18.2%)of patients who failed OFC ate the trigger food,and 9/18(50.0%)of patients who did not perform a tolerance OFC ate the trigger food.We have analyzed some possible influencing factors and no difference was found to be statistically significant.Our results are in line with those reported for IgE-mediated food allergies.As has already been proposed by others,we suggest reassessing food consumption in all patients after a food challenge.
文摘目的通过高通量测序分析食物蛋白诱导性直肠结肠炎(food protein-induced proctocolitis,FPIP)患儿肠道菌群特征。方法选取2018年10月至2021年2月遵义医科大学第三附属医院门诊就诊的小于6月龄纯母乳喂养发生FPIP患儿31例纳入FPIP组,正常健康婴儿31例纳入健康对照组;采集两组粪便样本提取DNA并通过PCR扩增,采用高通量测序分析粪便样品中的16S r DNA V3~V4片段并进行相关生物信息学分析。结果肠道菌群多样性分析示FPIP组菌群多样性Shannon指数低于健康对照组,但差异无统计学意义(P>0.05);菌群丰富度Chao指数高于健康对照组(P<0.01)。在门水平上,两组菌群组成均主要由厚壁菌门、放线菌门、变形菌门、拟杆菌门4个菌门构成;与健康对照组相比,FPIP组中放线菌门构成比显著降低(P<0.001),变形菌门构成比明显增加(P<0.05)。在属水平上,FPIP组主要由大肠埃希氏菌属、梭状芽孢杆菌属、肠球菌属、克雷伯氏菌属和双歧杆菌属组成;健康对照组主要由双歧杆菌属、链球菌属组成;与健康对照组相比,FPIP组中双歧杆菌属和瘤胃球菌属构成比明显降低(P<0.05),梭状芽孢杆菌属和志贺氏菌属构成比明显增加(P<0.05)。结论FPIP组与健康对照组相比,肠道菌群多样性下降,丰富度升高;在菌群组成结构上某些细菌菌属存在差异。提示在FPIP的发生发展过程中,肠道微生物群在属水平上的构成改变可能起到了重要作用。
