It is necessary to take measures against infectious diseases in the Southeast Asian prawn farming industry. In giant tiger prawn （Penaeus monodon） aquaculture, diseases caused by viruses such as white spot syndrome ...It is necessary to take measures against infectious diseases in the Southeast Asian prawn farming industry. In giant tiger prawn （Penaeus monodon） aquaculture, diseases caused by viruses such as white spot syndrome virus （WSSV） and bacteria such as Vibrio have become a menace. Appropriate treatments of prawn culture pond＇s waters are advocated for preventing and controlling pathogens. The purpose of this study was to conduct an antimicrobial water treatment using a low-voltage pulsed electric field sterilization. Here we prepared a mechanical low-voltage pulsed electric field system with copper wire coiling around a titanium ring. The viability of WSSV in seawater was examined by prawn infectivity experiments. We inoculated healthy prawns （approximately 10-15 g） with the WSSV master sample that passed 0-3 times through the system. WSSV infection in prawns decreased according to the number of passes through the system. Healthy prawns survived for〉 10 days without feeding, where as prawns inoculated with the WSSV master sample showed symptoms of white spot and died in about 4 days. Two-thirds of the prawns inoculated with the WSSV master sample that was passed once through the system developed WSSV symptoms and were polymerase chain reaction （PCR） positive. However, no symptoms were observed and prawns were PCR negative when the WSSV master sample was passed three times through the system. Based on these results, we purpose that a low-voltage pulsed electric field system will serve as an efficient pond drainage sterilization system and will replace conventional treatments using chemicals such as sodium hypochlorite.展开更多
This experiment designed the pulsed electric fields （PEF） of high intensity of 100 kV· cm^-1 sterilization system. Fluorescent pseudomonas as target cell was operated 180 s in the PEF. By observing the differen...This experiment designed the pulsed electric fields （PEF） of high intensity of 100 kV· cm^-1 sterilization system. Fluorescent pseudomonas as target cell was operated 180 s in the PEF. By observing the difference of the bacteria before and after the disposal by TEM, it is found that the cell walls of the treated bacteria were broken. Irreversible perforations were formed on the cell membrane. The cell inclusions and cell fragments were leaked. The cell died as a result. The results showed that the PEF sterilization system designed can be used for liquid food sterilization experiments.展开更多
脉冲电场(Pulsed Electric Field,PEF)技术是一种新兴非热食品加工技术,由于PEF对食品质量损伤小且能杀灭食品中有害微生物所以被广泛应用。PEF通过氧化应激途径激活植物类食品的营养物质合成途径,诱导营养物质合成、提高食品质量,然而...脉冲电场(Pulsed Electric Field,PEF)技术是一种新兴非热食品加工技术,由于PEF对食品质量损伤小且能杀灭食品中有害微生物所以被广泛应用。PEF通过氧化应激途径激活植物类食品的营养物质合成途径,诱导营养物质合成、提高食品质量,然而关于这一领域的研究较少。PEF发生了氧化应激反应造成了营养物质合成与积累,植物类食品受到PEF刺激后产生大量活性氧(Reactive Oxygen Species,ROS),ROS会激活代谢物合成途径最终合成蛋白、多酚、硫代葡萄糖苷和胡萝卜素等物质;ROS聚集在微生物的细胞膜上会造成蛋白表达异常,损害脂质层和脱氧核糖核酸(Deoxyribonucleic Acid,DNA),最终导致微生物失活。展开更多
作为一项重要的非热加工技术,高压脉冲电场(pulsed electric field,简称PEF)已成为目前食品杀菌与钝酶工艺研究中最为活跃的技术之一。随着PEF应用研究的不断深入和拓宽,PEF在物质定向改性、目标成分提取、食品干燥、食品冷冻与解冻、...作为一项重要的非热加工技术,高压脉冲电场(pulsed electric field,简称PEF)已成为目前食品杀菌与钝酶工艺研究中最为活跃的技术之一。随着PEF应用研究的不断深入和拓宽,PEF在物质定向改性、目标成分提取、食品干燥、食品冷冻与解冻、果蔬榨汁、酒类陈酿以及农药降解等方面的应用也越来越多,文中总结归纳了近年来PEF在这些方面的研究进展,以期为PEF技术的深入研究与广泛应用提供参考。展开更多
文摘It is necessary to take measures against infectious diseases in the Southeast Asian prawn farming industry. In giant tiger prawn （Penaeus monodon） aquaculture, diseases caused by viruses such as white spot syndrome virus （WSSV） and bacteria such as Vibrio have become a menace. Appropriate treatments of prawn culture pond＇s waters are advocated for preventing and controlling pathogens. The purpose of this study was to conduct an antimicrobial water treatment using a low-voltage pulsed electric field sterilization. Here we prepared a mechanical low-voltage pulsed electric field system with copper wire coiling around a titanium ring. The viability of WSSV in seawater was examined by prawn infectivity experiments. We inoculated healthy prawns （approximately 10-15 g） with the WSSV master sample that passed 0-3 times through the system. WSSV infection in prawns decreased according to the number of passes through the system. Healthy prawns survived for〉 10 days without feeding, where as prawns inoculated with the WSSV master sample showed symptoms of white spot and died in about 4 days. Two-thirds of the prawns inoculated with the WSSV master sample that was passed once through the system developed WSSV symptoms and were polymerase chain reaction （PCR） positive. However, no symptoms were observed and prawns were PCR negative when the WSSV master sample was passed three times through the system. Based on these results, we purpose that a low-voltage pulsed electric field system will serve as an efficient pond drainage sterilization system and will replace conventional treatments using chemicals such as sodium hypochlorite.
基金the Youth Science Fund of Heilongjiang Province (QC07C39)Postdoctoral Fund of Heilongjiang (LBH-Z06163)Science Research Fund of Northeast Agricultural University
文摘This experiment designed the pulsed electric fields （PEF） of high intensity of 100 kV· cm^-1 sterilization system. Fluorescent pseudomonas as target cell was operated 180 s in the PEF. By observing the difference of the bacteria before and after the disposal by TEM, it is found that the cell walls of the treated bacteria were broken. Irreversible perforations were formed on the cell membrane. The cell inclusions and cell fragments were leaked. The cell died as a result. The results showed that the PEF sterilization system designed can be used for liquid food sterilization experiments.
文摘作为一项重要的非热加工技术,高压脉冲电场(pulsed electric field,简称PEF)已成为目前食品杀菌与钝酶工艺研究中最为活跃的技术之一。随着PEF应用研究的不断深入和拓宽,PEF在物质定向改性、目标成分提取、食品干燥、食品冷冻与解冻、果蔬榨汁、酒类陈酿以及农药降解等方面的应用也越来越多,文中总结归纳了近年来PEF在这些方面的研究进展,以期为PEF技术的深入研究与广泛应用提供参考。
