BACKGROUND In traditional Chinese medicine(TCM),frankincense and myrrh are the main components of the antitumor drug Xihuang Pill.These compounds show anticancer activity in other biological systems.However,whether fr...BACKGROUND In traditional Chinese medicine(TCM),frankincense and myrrh are the main components of the antitumor drug Xihuang Pill.These compounds show anticancer activity in other biological systems.However,whether frankincense and/or myrrh can inhibit the occurrence of hepatocellular carcinoma(HCC)is unknown,and the potential molecular mechanism(s)has not yet been determined.AIM To predict and determine latent anti-HCC therapeutic targets and molecular mechanisms of frankincense and myrrh in vivo.METHODS In the present study,which was based on the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(http://tcmspw.com/tcmsp.php),Universal Protein database(http://www.uniprot.org),GeneCards:The Human Gene Database(http://www.genecards.org/)and Comparative Toxicogenomics Database(http://www.ctdbase.org/),the efficacy of and mechanism by which frankincense and myrrh act as anti-HCC compounds were predicted.The core prediction targets were screened by molecular docking.In vivo,SMMC-7721 human liver cancer cells were transplanted as xenografts into nude mice to establish a subcutaneous tumor model,and two doses of frankincense plus myrrh or one dose of an EGFR inhibitor was administered to these mice continuously for 14 d.The tumors were collected and evaluated:the tumor volume and growth rate were gauged to evaluate tumor growth;hematoxylineosin staining was performed to estimate histopathological changes;immunofluorescence(IF)was performed to detect the expression of CD31,α-SMA and collagen IV;transmission electron microscopy(TEM)was conducted to observe the morphological structure of vascular cells;enzyme-linked immunosorbent assay(ELISA)was performed to measure the levels of secreted HIF-1αand TNF-α;reverse transcription-polymerase chain reaction(RT-qPCR)was performed to measure the mRNA expression of HIF-1α,TNF-α,VEGF and MMP-9;and Western blot(WB)was performed to determine the levels of proteins expressed in the EGFR-mediated PI3K/Akt and MAPK signaling pathways.RESULTS The results of the network pharmacology analysis showed that there were 35 active components in the frankincense and myrrh extracts targeting 151 key targets.The molecular docking analysis showed that both boswellic acid and stigmasterol showed strong affinity for the targets,with the greatest affinity for EGFR.Frankincense and myrrh treatment may play a role in the treatment of HCC by regulating hypoxia responses and vascular system-related pathological processes,such as cytokine-receptor binding,and pathways,such as those involving serine/threonine protein kinase complexes and MAPK,HIF-1 and ErbB signaling cascades.The animal experiment results were verified.First,we found that,through frankincense and/or myrrh treatment,the volume of subcutaneously transplanted HCC tumors was significantly reduced,and the pathological morphology was attenuated.Then,IF and TEM showed that frankincense and/or myrrh treatment reduced CD31 and collagen IV expression,increased the coverage of perivascular cells,tightened the connection between cells,and improved the shape of blood vessels.In addition,ELISA,RT-qPCR and WB analyses showed that frankincense and/or myrrh treatment inhibited the levels of hypoxia-inducible factors,inflammatory factors and angiogenesis-related factors,namely,HIF-1α,TNF-α,VEGF and MMP-9.Furthermore,mechanistic experiments illustrated that the effect of frankincense plus myrrh treatment was similar to that of an EGFR inhibitor with regard to controlling EGFR activation,thereby inhibiting the phosphorylation activity of its downstream targets:the PI3K/Akt and MAPK(ERK,p38 and JNK)pathways.CONCLUSION In summary,frankincense and myrrh treatment targets tumor blood vessels to exert anti-HCC effects via EGFR-activated PI3K/Akt and MAPK signaling pathways,highlighting the potential of this dual TCM compound as an anti-HCC candidate.展开更多
Background:According to the traditional Chinese medicine theory,the common processing methods for Ruxiang(frankincense)include stir-frying processing and vinegar processing.Methods:With network pharmacology and therma...Background:According to the traditional Chinese medicine theory,the common processing methods for Ruxiang(frankincense)include stir-frying processing and vinegar processing.Methods:With network pharmacology and thermal analysis methods,we selected and identified the main and irritating ingredients,established the pyrolysis characteristic parameters for identifying frankincense quality,quantified the starting and optimum temperature limits for processing frankincense,and analyzed the influences of processing adjuvants(vinegar)on combustion pyrolysis characteristics of frankincense.By applying Fourier transformation infrared spectrometry,high performance liquid chromatography,gas chromatography and UV-visible spectrometry,we evaluated the processing technology developed in our study of frankincense and its processed products.Results:Based on network pharmacology,we can find that the classical compounds of essential oil are the main pharmacodynamics components of frankincense possessing the anti-inflammatory,analgesia,anti-tumor,anti-ulcer and ant-inflammatory bowel disease effects.The pyrolysis combustion rate peaks of frankincense at 285.27±7.05℃and 476.99±13.46℃were the characteristic peaks used to identify frankincense quality.The specific temperature of slow-fire processing,including the vinegar processing and stir-frying processing,was 148.72±5.09℃.Heating for 5.67±0.98 min after reaching processing temperature was needed for processing frankincense with vinegar.The kinetics analysis showed that both frankincense and its vinegar mixture exhibited good linear relationships.The feasibility and practicability for our processing technology were verified through the determination of medicinal compounds by Fourier transformation infrared spectrometry,high performance liquid chromatography,gas chromatography and UV.Conclusion:Frankincense essential oils were the main active components of frankincense,which also can be taken as the internal chemical control indexes to judge the quality control and to optimize the processing technology of frankincense.The pyrolysis combustion rate peaks of frankincense at 285.27±7.05℃and 476.99±13.46℃were the characteristic peaks that can be used to identify frankincense quality.The optimum processing conditions of vinegar-processed frankincense with were as follows:with vinegar,20%;heating temperature,148.72±5.09℃;and heating time,5.67±0.98 min.Under these conditions,frankincense essential oil could cause a moderate effect on combustion pyrolysis characteristics to exert its corresponding pharmacological effects.展开更多
Frankincense is also called“Fumigated land,”“Horsetail spice,”and“Godsend spice.”The alias“Fumigated land”shows its strong fragrance that can cover the whole land.The name of“Horsetail spice”implies its morp...Frankincense is also called“Fumigated land,”“Horsetail spice,”and“Godsend spice.”The alias“Fumigated land”shows its strong fragrance that can cover the whole land.The name of“Horsetail spice”implies its morphologic characteristics and mild effects,and another name of“Godsend spice”indicates its functions of relieving swelling and pain,healing sore,and growing muscle,as if it is the grace donated by God.The name of“Frankincense”displays its complex productive progress.With thick quality and strong fragrance,frankincense acts as daily supplies for religious activities and folk activities due to its low price and a wide range of uses in medicine and daily life.Frankincense shows the infinite charm of spice culture along the Silk Road from the distant Western Regions to the East.At the same time,it enriches the treasure house of traditional Chinese medicine and becomes one of the most popular spices in both eastern and western,nobility and common.展开更多
The discovery of novel antitumor agents derived from natural plants is a principal objective of anticancer drug research.Frankincense,a widely recognized natural antitumor medicine,has undergone a systematic review en...The discovery of novel antitumor agents derived from natural plants is a principal objective of anticancer drug research.Frankincense,a widely recognized natural antitumor medicine,has undergone a systematic review encompassing its species,chemical constituents,and diverse pharmacological activities and mechanisms.The different species of frankincense include Boswellia serrata,Somali frankincense,Boswellia frereana,and Boswellia arabica.Various frankincense extracts and compounds exhibit antitumor,anti-inflammatory,and hepatoprotective properties and antioxidation,memory enhancement,and immunological regulation capabilities.They also have comprehensive effects on regulating flora.Frankincense and its principal chemical constituents have demonstrated promising chemoprophylactic and therapeutic abilities against tumors.This review provides a systematic summary of the mechanism of action underlying the antitumor effects of frankincense and its major constituents,thus laying the foundations for developing effective tumor-combating targets.展开更多
Objective:To investigate the efficacy of frankincense and myrrha in the treatment of acute interstitial cystitis/painful bladder syndrome(IC/PBS).Methods:The effects of frankincense and myrrha on the proliferation and...Objective:To investigate the efficacy of frankincense and myrrha in the treatment of acute interstitial cystitis/painful bladder syndrome(IC/PBS).Methods:The effects of frankincense and myrrha on the proliferation and migration of primary human urothelial cells(HUCs)were assessed in vitro.In the animal study,48 virgin female rats were randomized into 4 groups(12 in each group):(1)control group(saline-injected control);(2)cyclophosphamide(CYP)group(intraperitoneal injected 150 mg/kg CYP);(3)CYP+pentosan polysulfate sodium group(orally received 50 mg/kg pentosan polysulfate sodium);and(4)CYP+frankincense and myrrha group[orally received frankincense(200 mg/kg)and myrrha(200 mg/kg)].Rats orally received pentosan polysulfate sodium or frankincense and myrrha on day 1,2,and 3.The experiments were performed on day 4.Pain and cystometry assessment behavior test were performed.Voiding interval values were assessed in rats under anesthesia.Finally,immunohistochemistry and Western blot were used to confirm the location and level,respectively,of cell junction-associated protein zonula occludens-2(ZO-2)expression.Results:Low dose frankincense and myrrha increased cell proliferation and migration in HUCs compared with control(P<0.05).Rats with acute IC/PBS rats exhibited lower voiding interval values,pain tolerance,and ZO-2 expression(P<0.05).Voiding interval values and pain tolerance were higher in the frankincense and myrrha group than CYP group(P<0.05).ZO-2 expression in the bladder was increased in the CYP+pentosan polysulfate and frankincense+myrrha groups compared with the CYP-induced acute IC/PBS group(P<0.05).Conclusion:frankincense and myrrha modulate urothelial wound healing,which ameliorates typical features of acute IC/PBS in rats.展开更多
Background:As the demand for traditional Chinese medicinal materials increases in China and even the world,there is an urgent need for an effective and simple identification technology to identify the origin and quali...Background:As the demand for traditional Chinese medicinal materials increases in China and even the world,there is an urgent need for an effective and simple identification technology to identify the origin and quality of the latter and ensure the safety of clinical medication.Mineral element analysis and isotope finger-printing are the two commonly used techniques in traditional origin identification.Both of these techniques require the use of stoichiometric methods in the identification process.Although they have high accuracy and sensitivity,they are expensive and inefficient.In addition,near-infrared spectroscopy is a fast,nondestructive,and widely used identification technique developed in recent years,but its identification results are susceptible to samples’states and environmental conditions,and its sensitivity is low.Hyperspectral imaging combines the advantages of imaging technology and optical technology,which can simultaneously access the image information and spectral information which reflect the external characteristics,internal physical structure,and chemical composition of the samples.Hyperspectral imaging is widely applied to agricultural product inspection,but research into its application in origin and quality identification of TCM materials is rare.Methods:In this study,the algorithm framework discriminative marginalized least squares regression(DMLSR)was used for feature extraction of frankincense hyperspectral data.The DMLSR with intraclass compactness graph and manifold regularization can efficiently learn the projective samples with higher separability and less redundant information than the original samples.Then,the discriminative collaborative representation with Tikhonov regularization(DCRT)was applied for classifying the geographical origin and level of frankincense.DCRT introduces the discriminant regularization term and incorporates SID,which is more sensitive to the spectrum as the measurement method and is more suitable for the frankincense spectral data compared with SVM.Results:For the origin classification task,samples of all levels from each origin were,respectively,selected for three-way classification.We used 10-fold cross-validation to select a model parameter in the experiment.When obtaining the optimal parameters,we randomly selected the training set and testing set,where the training set accounts for 70%and the training set for 30%.After repeating this random process 10 times,we obtained the final average classification accuracy,which is higher than 90%,and the standard deviation fluctuation is usually small.For the level classification task,samples of each level from three origins were separately selected for multiclassification.We randomly selected the training set and testing set from each origin.The level classification results of the three origins are good on D4350 data,and the classification accuracy of each level is basically above 80%.Conclusion:Experiments and analysis show that our algorithm framework has excellent classification performance,which is stable in origin classification and has potential for generalization.In addition,the experiments show that in our algorithm framework,different classification tasks need to combine different data sources to achieve better classification and recognition,as the origin classification task uses frankincense’s D3000 data,and level classification task uses frankincense’s D4350 data.展开更多
Boswellia sacra from Burseraceae family, grows is limited and the land unsuitable for traditional agriculture wild in dry hot lands of southern part of Oman （Dhofar）, where the water purpose. Boswellia sacra is a mu...Boswellia sacra from Burseraceae family, grows is limited and the land unsuitable for traditional agriculture wild in dry hot lands of southern part of Oman （Dhofar）, where the water purpose. Boswellia sacra is a multipurpose tree, but more famous for its high value olibanum. The olibanum is used for ritual and in church ceremonies worldwide, traditional medicines, pharmaceutical, perfumery, cosmetics, chewing, painting, food flavouring. In addition to this, the species has other numerous environmental, historical, and socio-economical benefits. This study is based on literature reviews, and on the interview local people, as well as on personal field experiences. The objective is to introduce the importance of the species and to study the native habitats, the distribution, and the harvesting techniques. The soil is carbonitic, isohypertherrnic, lithic torriothent, poor in mineral elements. The olibanum produces when the tree tapped by making a thin vertical slicing into the tree trunk. Initial size should be about 18 cm2 （3 × 6 cm） and 1-2 mm in depth and the diameter tree trunk should be not less than 10 cm. Tree tapping should be stopped 2-3 weeks before the rain season. The method of tree tapping and olibanum collection need to be revised for long-term tree protection.展开更多
One of the leading causes of death worldwide is cardiovascular disease, hence searching for a cure is an important endeavor. The totally safe, edible, and inexpensive Boswellia plant exudate, known as olibanum or fran...One of the leading causes of death worldwide is cardiovascular disease, hence searching for a cure is an important endeavor. The totally safe, edible, and inexpensive Boswellia plant exudate, known as olibanum or frankincense, is considered to possess diverse medicinal values in traditional medicine and from recent biological studies. Investigating the cardioprotective and antioxidant activities of olibanum from a Bo~＇wellia species, family Bursearaceae, namely Boswellia carteri Birdw. was the aim of this study. Cardioprotective activity was evaluated using a model of myocardial infarction induced by isoprenaline （ISO）, while antioxidant activity was tested adopting nitric oxide scavenging （NOS） and azino-bis-3-ethyl benzthiazoline-6-sulfonic acid （ABTS） assays. The results revealed a mild cardioprotecfive effect and weak antioxidant activity.展开更多
基金the National Natural Science Foundation of China,No.U20A20408(Major Program)and No.82074450(General Program)Natural Science Foundation of Hunan Province,No.2020JJ4066+2 种基金Hunan Province Research and innovation projects for Postgraduates,No.CX20190541Hunan Province"domestic firstclass cultivation discipline"Integrated Traditional Chinese and Western medicine open fund project,No.2018ZXYJH03Hunan University Undergraduate Research Learning and Innovative Experiment Project,No.201609030114.
文摘BACKGROUND In traditional Chinese medicine(TCM),frankincense and myrrh are the main components of the antitumor drug Xihuang Pill.These compounds show anticancer activity in other biological systems.However,whether frankincense and/or myrrh can inhibit the occurrence of hepatocellular carcinoma(HCC)is unknown,and the potential molecular mechanism(s)has not yet been determined.AIM To predict and determine latent anti-HCC therapeutic targets and molecular mechanisms of frankincense and myrrh in vivo.METHODS In the present study,which was based on the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(http://tcmspw.com/tcmsp.php),Universal Protein database(http://www.uniprot.org),GeneCards:The Human Gene Database(http://www.genecards.org/)and Comparative Toxicogenomics Database(http://www.ctdbase.org/),the efficacy of and mechanism by which frankincense and myrrh act as anti-HCC compounds were predicted.The core prediction targets were screened by molecular docking.In vivo,SMMC-7721 human liver cancer cells were transplanted as xenografts into nude mice to establish a subcutaneous tumor model,and two doses of frankincense plus myrrh or one dose of an EGFR inhibitor was administered to these mice continuously for 14 d.The tumors were collected and evaluated:the tumor volume and growth rate were gauged to evaluate tumor growth;hematoxylineosin staining was performed to estimate histopathological changes;immunofluorescence(IF)was performed to detect the expression of CD31,α-SMA and collagen IV;transmission electron microscopy(TEM)was conducted to observe the morphological structure of vascular cells;enzyme-linked immunosorbent assay(ELISA)was performed to measure the levels of secreted HIF-1αand TNF-α;reverse transcription-polymerase chain reaction(RT-qPCR)was performed to measure the mRNA expression of HIF-1α,TNF-α,VEGF and MMP-9;and Western blot(WB)was performed to determine the levels of proteins expressed in the EGFR-mediated PI3K/Akt and MAPK signaling pathways.RESULTS The results of the network pharmacology analysis showed that there were 35 active components in the frankincense and myrrh extracts targeting 151 key targets.The molecular docking analysis showed that both boswellic acid and stigmasterol showed strong affinity for the targets,with the greatest affinity for EGFR.Frankincense and myrrh treatment may play a role in the treatment of HCC by regulating hypoxia responses and vascular system-related pathological processes,such as cytokine-receptor binding,and pathways,such as those involving serine/threonine protein kinase complexes and MAPK,HIF-1 and ErbB signaling cascades.The animal experiment results were verified.First,we found that,through frankincense and/or myrrh treatment,the volume of subcutaneously transplanted HCC tumors was significantly reduced,and the pathological morphology was attenuated.Then,IF and TEM showed that frankincense and/or myrrh treatment reduced CD31 and collagen IV expression,increased the coverage of perivascular cells,tightened the connection between cells,and improved the shape of blood vessels.In addition,ELISA,RT-qPCR and WB analyses showed that frankincense and/or myrrh treatment inhibited the levels of hypoxia-inducible factors,inflammatory factors and angiogenesis-related factors,namely,HIF-1α,TNF-α,VEGF and MMP-9.Furthermore,mechanistic experiments illustrated that the effect of frankincense plus myrrh treatment was similar to that of an EGFR inhibitor with regard to controlling EGFR activation,thereby inhibiting the phosphorylation activity of its downstream targets:the PI3K/Akt and MAPK(ERK,p38 and JNK)pathways.CONCLUSION In summary,frankincense and myrrh treatment targets tumor blood vessels to exert anti-HCC effects via EGFR-activated PI3K/Akt and MAPK signaling pathways,highlighting the potential of this dual TCM compound as an anti-HCC candidate.
基金funded by Natural Science Foundation of Shanxi Province,China(Grant No.2014ZD0302)Science and Technology Innovation Ability Cultivation Plan of Shanxi University of Chinese Medicine(2019PY-154)Research Project of Health Commission of Shanxi Province(2019092)。
文摘Background:According to the traditional Chinese medicine theory,the common processing methods for Ruxiang(frankincense)include stir-frying processing and vinegar processing.Methods:With network pharmacology and thermal analysis methods,we selected and identified the main and irritating ingredients,established the pyrolysis characteristic parameters for identifying frankincense quality,quantified the starting and optimum temperature limits for processing frankincense,and analyzed the influences of processing adjuvants(vinegar)on combustion pyrolysis characteristics of frankincense.By applying Fourier transformation infrared spectrometry,high performance liquid chromatography,gas chromatography and UV-visible spectrometry,we evaluated the processing technology developed in our study of frankincense and its processed products.Results:Based on network pharmacology,we can find that the classical compounds of essential oil are the main pharmacodynamics components of frankincense possessing the anti-inflammatory,analgesia,anti-tumor,anti-ulcer and ant-inflammatory bowel disease effects.The pyrolysis combustion rate peaks of frankincense at 285.27±7.05℃and 476.99±13.46℃were the characteristic peaks used to identify frankincense quality.The specific temperature of slow-fire processing,including the vinegar processing and stir-frying processing,was 148.72±5.09℃.Heating for 5.67±0.98 min after reaching processing temperature was needed for processing frankincense with vinegar.The kinetics analysis showed that both frankincense and its vinegar mixture exhibited good linear relationships.The feasibility and practicability for our processing technology were verified through the determination of medicinal compounds by Fourier transformation infrared spectrometry,high performance liquid chromatography,gas chromatography and UV.Conclusion:Frankincense essential oils were the main active components of frankincense,which also can be taken as the internal chemical control indexes to judge the quality control and to optimize the processing technology of frankincense.The pyrolysis combustion rate peaks of frankincense at 285.27±7.05℃and 476.99±13.46℃were the characteristic peaks that can be used to identify frankincense quality.The optimum processing conditions of vinegar-processed frankincense with were as follows:with vinegar,20%;heating temperature,148.72±5.09℃;and heating time,5.67±0.98 min.Under these conditions,frankincense essential oil could cause a moderate effect on combustion pyrolysis characteristics to exert its corresponding pharmacological effects.
文摘Frankincense is also called“Fumigated land,”“Horsetail spice,”and“Godsend spice.”The alias“Fumigated land”shows its strong fragrance that can cover the whole land.The name of“Horsetail spice”implies its morphologic characteristics and mild effects,and another name of“Godsend spice”indicates its functions of relieving swelling and pain,healing sore,and growing muscle,as if it is the grace donated by God.The name of“Frankincense”displays its complex productive progress.With thick quality and strong fragrance,frankincense acts as daily supplies for religious activities and folk activities due to its low price and a wide range of uses in medicine and daily life.Frankincense shows the infinite charm of spice culture along the Silk Road from the distant Western Regions to the East.At the same time,it enriches the treasure house of traditional Chinese medicine and becomes one of the most popular spices in both eastern and western,nobility and common.
基金Supported by the Youth Project of National Natural Science Foundation of China(No.82104861)National Natural Science Foundation of China(Nos.U20A20408 and 82074450)+1 种基金Natural Science Foundation of Hunan Province of China(Nos.2021JJ40408,2021JJ40420)Hunan Provincial Department of Education General Project(No.20C1407)。
文摘The discovery of novel antitumor agents derived from natural plants is a principal objective of anticancer drug research.Frankincense,a widely recognized natural antitumor medicine,has undergone a systematic review encompassing its species,chemical constituents,and diverse pharmacological activities and mechanisms.The different species of frankincense include Boswellia serrata,Somali frankincense,Boswellia frereana,and Boswellia arabica.Various frankincense extracts and compounds exhibit antitumor,anti-inflammatory,and hepatoprotective properties and antioxidation,memory enhancement,and immunological regulation capabilities.They also have comprehensive effects on regulating flora.Frankincense and its principal chemical constituents have demonstrated promising chemoprophylactic and therapeutic abilities against tumors.This review provides a systematic summary of the mechanism of action underlying the antitumor effects of frankincense and its major constituents,thus laying the foundations for developing effective tumor-combating targets.
基金Supported in part by China Medical University Hospital(No.DMR-109-085)Taiwan Ministry of Science and Technology(No.MOST 107-2314-B-039-036 and MOST 107-2320-B-039-034)。
文摘Objective:To investigate the efficacy of frankincense and myrrha in the treatment of acute interstitial cystitis/painful bladder syndrome(IC/PBS).Methods:The effects of frankincense and myrrha on the proliferation and migration of primary human urothelial cells(HUCs)were assessed in vitro.In the animal study,48 virgin female rats were randomized into 4 groups(12 in each group):(1)control group(saline-injected control);(2)cyclophosphamide(CYP)group(intraperitoneal injected 150 mg/kg CYP);(3)CYP+pentosan polysulfate sodium group(orally received 50 mg/kg pentosan polysulfate sodium);and(4)CYP+frankincense and myrrha group[orally received frankincense(200 mg/kg)and myrrha(200 mg/kg)].Rats orally received pentosan polysulfate sodium or frankincense and myrrha on day 1,2,and 3.The experiments were performed on day 4.Pain and cystometry assessment behavior test were performed.Voiding interval values were assessed in rats under anesthesia.Finally,immunohistochemistry and Western blot were used to confirm the location and level,respectively,of cell junction-associated protein zonula occludens-2(ZO-2)expression.Results:Low dose frankincense and myrrha increased cell proliferation and migration in HUCs compared with control(P<0.05).Rats with acute IC/PBS rats exhibited lower voiding interval values,pain tolerance,and ZO-2 expression(P<0.05).Voiding interval values and pain tolerance were higher in the frankincense and myrrha group than CYP group(P<0.05).ZO-2 expression in the bladder was increased in the CYP+pentosan polysulfate and frankincense+myrrha groups compared with the CYP-induced acute IC/PBS group(P<0.05).Conclusion:frankincense and myrrha modulate urothelial wound healing,which ameliorates typical features of acute IC/PBS in rats.
文摘Background:As the demand for traditional Chinese medicinal materials increases in China and even the world,there is an urgent need for an effective and simple identification technology to identify the origin and quality of the latter and ensure the safety of clinical medication.Mineral element analysis and isotope finger-printing are the two commonly used techniques in traditional origin identification.Both of these techniques require the use of stoichiometric methods in the identification process.Although they have high accuracy and sensitivity,they are expensive and inefficient.In addition,near-infrared spectroscopy is a fast,nondestructive,and widely used identification technique developed in recent years,but its identification results are susceptible to samples’states and environmental conditions,and its sensitivity is low.Hyperspectral imaging combines the advantages of imaging technology and optical technology,which can simultaneously access the image information and spectral information which reflect the external characteristics,internal physical structure,and chemical composition of the samples.Hyperspectral imaging is widely applied to agricultural product inspection,but research into its application in origin and quality identification of TCM materials is rare.Methods:In this study,the algorithm framework discriminative marginalized least squares regression(DMLSR)was used for feature extraction of frankincense hyperspectral data.The DMLSR with intraclass compactness graph and manifold regularization can efficiently learn the projective samples with higher separability and less redundant information than the original samples.Then,the discriminative collaborative representation with Tikhonov regularization(DCRT)was applied for classifying the geographical origin and level of frankincense.DCRT introduces the discriminant regularization term and incorporates SID,which is more sensitive to the spectrum as the measurement method and is more suitable for the frankincense spectral data compared with SVM.Results:For the origin classification task,samples of all levels from each origin were,respectively,selected for three-way classification.We used 10-fold cross-validation to select a model parameter in the experiment.When obtaining the optimal parameters,we randomly selected the training set and testing set,where the training set accounts for 70%and the training set for 30%.After repeating this random process 10 times,we obtained the final average classification accuracy,which is higher than 90%,and the standard deviation fluctuation is usually small.For the level classification task,samples of each level from three origins were separately selected for multiclassification.We randomly selected the training set and testing set from each origin.The level classification results of the three origins are good on D4350 data,and the classification accuracy of each level is basically above 80%.Conclusion:Experiments and analysis show that our algorithm framework has excellent classification performance,which is stable in origin classification and has potential for generalization.In addition,the experiments show that in our algorithm framework,different classification tasks need to combine different data sources to achieve better classification and recognition,as the origin classification task uses frankincense’s D3000 data,and level classification task uses frankincense’s D4350 data.
文摘Boswellia sacra from Burseraceae family, grows is limited and the land unsuitable for traditional agriculture wild in dry hot lands of southern part of Oman （Dhofar）, where the water purpose. Boswellia sacra is a multipurpose tree, but more famous for its high value olibanum. The olibanum is used for ritual and in church ceremonies worldwide, traditional medicines, pharmaceutical, perfumery, cosmetics, chewing, painting, food flavouring. In addition to this, the species has other numerous environmental, historical, and socio-economical benefits. This study is based on literature reviews, and on the interview local people, as well as on personal field experiences. The objective is to introduce the importance of the species and to study the native habitats, the distribution, and the harvesting techniques. The soil is carbonitic, isohypertherrnic, lithic torriothent, poor in mineral elements. The olibanum produces when the tree tapped by making a thin vertical slicing into the tree trunk. Initial size should be about 18 cm2 （3 × 6 cm） and 1-2 mm in depth and the diameter tree trunk should be not less than 10 cm. Tree tapping should be stopped 2-3 weeks before the rain season. The method of tree tapping and olibanum collection need to be revised for long-term tree protection.
文摘One of the leading causes of death worldwide is cardiovascular disease, hence searching for a cure is an important endeavor. The totally safe, edible, and inexpensive Boswellia plant exudate, known as olibanum or frankincense, is considered to possess diverse medicinal values in traditional medicine and from recent biological studies. Investigating the cardioprotective and antioxidant activities of olibanum from a Bo~＇wellia species, family Bursearaceae, namely Boswellia carteri Birdw. was the aim of this study. Cardioprotective activity was evaluated using a model of myocardial infarction induced by isoprenaline （ISO）, while antioxidant activity was tested adopting nitric oxide scavenging （NOS） and azino-bis-3-ethyl benzthiazoline-6-sulfonic acid （ABTS） assays. The results revealed a mild cardioprotecfive effect and weak antioxidant activity.
