An EMS(ethy methanesulfonate)-induced lethal etiolated(le)mutant obtained from the rice variety Zhongjian 100 was characterized by lethal etiolated phenotypes,with significantly reduced levels of chlorophyll a,chlorop...An EMS(ethy methanesulfonate)-induced lethal etiolated(le)mutant obtained from the rice variety Zhongjian 100 was characterized by lethal etiolated phenotypes,with significantly reduced levels of chlorophyll a,chlorophyll b,total chlorophyll,and carotenoids.Additionally,the mutant displayed a significantly decreased number of chloroplast grana,along with irregular and less-stacked grana lamellae.The le mutant showed markedly diminished root length,root surface area,and root volume compared with the wild type.It also exhibited significantly lower catalase activity and total protein content,while peroxidase activity was significantly higher.Using the map-based cloning method,we successfully mapped the LE gene to a 48-kb interval between markers RM16107 and RM16110 on rice chromosome 3.A mutation(from T to C)was identified at nucleotide position 692 bp of LOC_Os03g59640(ChlD),resulting in a change from leucine to proline.By crossing HM133(a pale green mutant with a single-base substitution of A for G in exon 10 of ChlD subunit)with a heterozygous line of le(LEle),we obtained two plant lines heterozygous at both the LE and HM133 loci.Among 15 transgenic plants,3 complementation lines displayed normal leaf color with significantly higher total chlorophyll,chlorophyll a,and chlorophyll b contents.The mutation in le led to a lethal etiolated phenotype,which has not been observed in other ChlD mutants.The mutation in the AAA+domain of ChlD disrupted the interaction of ChlDle with ChlI as demonstrated by a yeast two-hybrid assay,leading to the loss of ChlD function and hindering chlorophyll synthesis and chloroplast development.Consequently,this disruption is responsible for the lethal etiolated phenotype in the mutant.展开更多
AIM： TO investigate the protein profile of human hepatocarcinoma cell line SMMC-7721, to analyze the specific functions of abundant expressed proteins in the processes of hepatocarcinoma genesis, growth and metastasi...AIM： TO investigate the protein profile of human hepatocarcinoma cell line SMMC-7721, to analyze the specific functions of abundant expressed proteins in the processes of hepatocarcinoma genesis, growth and metastasis, to identify the hepatocarcinoma-specific biomarkers for the early prediction in diagnosis, and to explore the new drug targets for liver cancer therapy. METHODS： Total proteins from human hepatocarcinoma cell line SMMC-7721 were separated by two-dimensional electrophoresis （2DE）. The silver-stained gel was analyzed by 2DE software Image Master 2D Elite. Interesting protein spots were identified by peptide mass fingerprinting based on matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry （MALDI-TOF-MS） and database searching. RESULTS： We obtained protein profile of human hepatocarcinoma cell line SMMC-7721. Among the twenty-one successfully identified proteins, mitofilin, endoplasmic reticulum protein ERp29, ubiquinol-cytochrome C reductase complex core protein I, peroxisomal enoyl CoA hydratase, peroxiredoxin-4 and probable 3-oxoacid CoA transferase 1 precursor were the six novel proteins identified in human hepatocarcinoma cells or tissues. Specific functions of the identified heat-shock proteins were analyzed in detail, and the results suggested that these proteins might promote tumorigenesis via inhibiting cell death induced by several cancer-related stresses or via inhibiting apoptosis at multiple points in the apoptotic signal pathway. Other identified chaperones and cancer-related proteins were also analyzed.CONCLUSION： Based on the protein profile of SMMC-7721 cells, functional analysis suggests that the identified chaperones and cancer-related proteins have their own pathways to contribute to the tumorigenesis, tumor growth and metastasis of liver cancer. Furthermore, proteomic analysis is indicated to be feasible in the cancer study.展开更多
This paper reported firstly successful cloning of lycopene ε-cyclase （lbLCYe） gene from sweetpotato, lpomoea batatas （L.） Lam. Using rapid amplification of cDNA ends （RACE）, lbLCYe gene was cloned from sweetpot...This paper reported firstly successful cloning of lycopene ε-cyclase （lbLCYe） gene from sweetpotato, lpomoea batatas （L.） Lam. Using rapid amplification of cDNA ends （RACE）, lbLCYe gene was cloned from sweetpotato cv. Nongdafu 14 with high carotenoid content. The 1 805 bp cDNA sequence oflbLCYe gene contained a 1236 bp open reading frame （ORF） encoding a 411 amino acids polypeptide with a molecular weight of 47 kDa and an isoelectric point （pI） of 6.95. IbLCYe protein contained one potential lycopene ε-cyclase domain and one potential FAD （flavinadenine dinucleotide）/NAD（P） （nicotinamide adenine dinucleotide phosphate）-binding domain, indicating that this protein shares the typical characteristics of LCYe proteins. The gDNA oflbLCYe gene was 4 029 bp and deduced to contain 5 introns and 6 exons. Real-time quantitative PCR analysis revealed that the expression level of IbLCYe gene was significantly higher in the storage roots of Nongdafu 14 than those in the leaves and stems. Transgenic tobacco （cv. Wisconsin 38） expressing [bLCYe gene accumulated significantly more ^-carotene compared to the untransformed control plants. These results showed that lbLCYe gene has an important function for the accumulation of carotenoids of sweetpotato.展开更多
Fiber cell initiation is a complex process involving many pathways,including phytohormones and components for transcriptional and posttranscriptional regulation.Here we report expression
Phenylalanine ammonia lyase(PAL)is the rate-limiting and pivotal enzyme of the general phenylpropanoid path-way,but few reports have been found on PAL genes in Pinus yunnanensis.In the present study,three PAL genes we...Phenylalanine ammonia lyase(PAL)is the rate-limiting and pivotal enzyme of the general phenylpropanoid path-way,but few reports have been found on PAL genes in Pinus yunnanensis.In the present study,three PAL genes were cloned and identified from P.yunnanensis seedlings for thefirst time,namely,PyPAL-1,PyPAL-2,and PyPAL-3.Our results indicated that the open-reading frames of PyPAL genes were 2184,2157,and 2385 bp.Phylogenetic tree analysis revealed that PyPALs have high homology with other known PAL genes in other plants.In vitro enzymatic analysis showed that all three PyPAL recombinant proteins could catalyze the deamination of L-phenylalanine to form trans-cinnamic acid,but only PAL1 and PAL2 can catalyze the conversion of L-tyrosine toρ-coumaric acid.Three PyPAL genes were expressed in different tissues in 1-year-old P.yunnanensis,and such genes had different expression patterns.This study lays a foundation for further understanding of the biosynthesis of secondary metabolites in P.yunnanensis.展开更多
Since entering the era of Industry 4.0,the concept of Healthcare 4.0 has also been put forward and explored by researchers.How to use Information Technology(IT)to better serve people’s healthcare is one of the most f...Since entering the era of Industry 4.0,the concept of Healthcare 4.0 has also been put forward and explored by researchers.How to use Information Technology(IT)to better serve people’s healthcare is one of the most featured emerging directions in the academic circle.An important field of Healthcare 4.0 research is the reliability engineering of healthcare service.Because healthcare systems often affect the health and even life of their users,developers must be very cautious in the design,development,and operation of these healthcare systems and services.The problems to be solved include the reliability of business process,system functions,and personal healthcare data.The Functional Resonance Analysis Method(FRAM)has been applied in reliability engineering for safety-critical systems in available studies,using both qualitative and quantitative approaches.However,the method has not been applied in the field of digital healthcare services development.Therefore,to narrow the gap,we present in this paper a semi-quantitative functional resonance analysis method to develop reliable healthcare services for diabetics.Moreover,this paper has tried to improve the reliability design of the service-oriented architecture(SOA)of traditional insulin pump therapy by system thinking.展开更多
Human living would be impossible without air quality. Consistent advancements in practically every aspect of contemporary human life have harmed air quality. Everyday industrial, transportation, and home activities tu...Human living would be impossible without air quality. Consistent advancements in practically every aspect of contemporary human life have harmed air quality. Everyday industrial, transportation, and home activities turn up dangerous contaminants in our surroundings. This study investigated two years’ worth of air quality and outlier detection data from two Indian cities. Studies on air pollution have used numerous types of methodologies, with various gases being seen as a vector whose components include gas concentration values for each observation per-formed. We use curves to represent the monthly average of daily gas emissions in our technique. The approach, which is based on functional depth, was used to find outliers in the city of Delhi and Kolkata’s gas emissions, and the outcomes were compared to those from the traditional method. In the evaluation and comparison of these models’ performances, the functional approach model studied well.展开更多
Four samples were chosen by systematic sampling from the range-finders between 1987 and 2003 to test the range-finders’ representativeness of the scoring criteria of CET-4 compositions. The samples were analyzed from...Four samples were chosen by systematic sampling from the range-finders between 1987 and 2003 to test the range-finders’ representativeness of the scoring criteria of CET-4 compositions. The samples were analyzed from the perspectives of transitivity, modality, thematic structure and cohesion. Fisher's Exact Test from the SAS system was used to compare two-sidedly the correct rate and the constituent ratio of these functional usages between two neighboring levels. Statistical results indicate that it is the quality, not the quantity of these functional usages that distinguishes two neighboring levels. The range-finders can basically represent the abstract scoring criteria as far as the four functional systems are concerned. However, the validity of CET-4 writing needs to be improved.展开更多
Background:Systemic lupus erythematosus(SLE)is an autoimmune disease that can affect multiple systems.Sjogren's syndrome(SS)is an autoimmune disease that may be primary SS(pSS)or occur together with other autoimmu...Background:Systemic lupus erythematosus(SLE)is an autoimmune disease that can affect multiple systems.Sjogren's syndrome(SS)is an autoimmune disease that may be primary SS(pSS)or occur together with other autoimmune diseases,including SLE.This study aimed to explore the shared gene signatures in SLE and pSS.Methods:Gene expression data sets of SLE(GSE50772 and GSE81622)and pSS(GSE84844 and GSE48378)were obtained and analyzed for differentially expressed genes(DEGs)in peripheral blood mononuclear cells(PBMCs).A protein–protein interaction(PPI)network was constructed.Gene ontology(GO)and KEGG pathway enrichment analysis were carried out for the DEGs.Results:We screened 232 and 110 DEGs from the SLE and pSS data sets,respectively.We found 32 shared DEGs,which were all upregulated in patients compared with controls.Among these 32 DEGs,11 genes showed a more than twofold change in all data sets(IFI27,IFI44L,RSAD2,IFIT1,IFI44,USP18,IFI6,HERC5,EPSTI1,OAS1,and OAS3).PPI analysis showed that 29 genes interacted with each other.GO analysis showed that these 32 shared DEGs were mainly enriched in biological processes associated with the type Ⅰ interferon signaling pathway,defense response to viruses,response to viruses,negative regulation of viral genome replication,and the immune response.Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that these 32 DEGs were related to virus infection.Conclusion:This study showed that alterations to biological processes associated with the response to virus infection play critical roles in both SLE and pSS.展开更多
Potentilla sericea is a heavy metal hyperaccumulator landscaping plant.MYB transcription factors play an important role in regulating plant stress response to adversity.However,there are few studies on MYB transcripti...Potentilla sericea is a heavy metal hyperaccumulator landscaping plant.MYB transcription factors play an important role in regulating plant stress response to adversity.However,there are few studies on MYB transcription factors in stress tolerance in Potentilla sericea.In this study,the PsMYB62 gene was successfully cloned from Potentilla sericea.Methods:Bioinformatic analysis and real-time quantitative PCR(qPCR)methods were used to evaluate this gene.The transgenic A.thaliana were obtained by flower dipping and the gene function was identified by determining physiological indicators under cadmium stress.Results:The open reading frame of PsMYB62 is 942 bp,which encodes 313 amino acids(aa)and belongs to the R2R3 MYB transcription factor.The plant overexpression vector PBI121-PsMYB62-GFP was constructed and successfully transferred into A.thaliana.The relative expression level of PsMYB62 was significantly increased by CdCl_(2),NaCl,ABA,and mannitol treatments.The germination rate of transgenic seeds was higher than those of wild type(WT)and empty vector(EV)under different concentrations of cadmium treatment.Upon treatment with 100μmol·L^(−1)of CdCl_(2)·2.5H_(2)O,the activities of superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)in the transgenic plants were significantly higher than those in the WT and EV.The contents of H_(2)O_(2),O_(2)·−and malondialdehyde(MDA)in transgenic lines were increased,but lower than those in WT and EV.The expression levels of AtGSH,AtPCS,and AtNAS4 that were related to the regulation of cadmium were increased,but the expression levels of transgenic lines were higher than those of WT and EV.Conclusion:The above results showed that PsMYB62 could be induced by cadmium and could improve the cadmium resistance of plants.展开更多
A MADS-box gene,CiMADS9,was cloned from the male flowers of Carya illinoinensis by rapid amplification of cDNA ends. The gene was 1 077 bp with a 768 bp open reading frame encoding 255 amino acids. Multiple sequence c...A MADS-box gene,CiMADS9,was cloned from the male flowers of Carya illinoinensis by rapid amplification of cDNA ends. The gene was 1 077 bp with a 768 bp open reading frame encoding 255 amino acids. Multiple sequence comparisons revealed that Ci MADS9 is a typical MIKC-type MADS-box gene with a MADS-box domain and a K semi-conserved region. Phylogenetic analysis indicated that CiMADS9 belongs to the AGL15 group of the MADS-box gene family. Quantitative reverse transcription polymerase chain reaction analysis indicated that the expression levels in reproductive organs(i.e.,flowers and young fruits) were considerably higher than in vegetative tissues(i.e.,leaves and branches). The highest expression levels were observed in male flowers. An overexpression vector for CiMADS9 was constructed and the gene was inserted into the Arabidopsis thaliana genome. CiMADS9 expression was confirmed in all transgenic lines. Compared with wild-type plants,transgenic A. thaliana plants overexpressing CiMADS9 exhibited delayed flowering and an increased number of leaves.展开更多
Within a framework of Halliday＇s SFL （Systemic Functional Linguistics）, this paper takes a functional approach to the analysis of the fairy tale--＂The Little Match-Seller＂ （Andersen, 1846）. The analysis is carr...Within a framework of Halliday＇s SFL （Systemic Functional Linguistics）, this paper takes a functional approach to the analysis of the fairy tale--＂The Little Match-Seller＂ （Andersen, 1846）. The analysis is carried out in three respects： genre, register and language. Genre analysis indicates that the schematic structure of the fairy tale is very much in line with the one mentioned by Hasan. Register analysis shows that the material process is the most dominant one; the Finite in the clauses is predominantly in past tenses, and most of the Themes in the text are unmarked ones. Language analysis reveals that the lexical density in the fairy tale is relatively low. The findings in this paper can help deepen people＇s understanding of the textual features of English fairy tales.展开更多
As two main approaches to analyzing literary discourses,literary criticism and stylistic analysis have been employed for a long period of time.With the application of the theories of Functional Grammar,this paper is w...As two main approaches to analyzing literary discourses,literary criticism and stylistic analysis have been employed for a long period of time.With the application of the theories of Functional Grammar,this paper is written for the purpose of being helpful for the readers of Dylan Thomas’poem“Do not Go Gentle into That Good Night”to understand the poem’s theme better as well as appreciate the artistic adaptation of language.Furthermore,because the poem is analyzed with the application of Functional Grammar,it helps the readers to appreciate“Do not Go Gentle into That Good Night”from a new perspective.展开更多
An orthotropic functionally graded piezoelectric rectangular plate with arbitrarily distributed material properties was studied, which is simply supported and grounded（electrically） on its four lateral edges. The st...An orthotropic functionally graded piezoelectric rectangular plate with arbitrarily distributed material properties was studied, which is simply supported and grounded（electrically） on its four lateral edges. The state equations of the functionally graded piezoelectric material were obtained using the state-space approach, and a Peano-Baker series solution was obtained for the coupled electroelastic fi elds of the functionally graded piezoelectric plate subjected to mechanical and electric loading on its upper and lower surfaces. The influence of different distributions of material properties on the structural response of the plate was studied using the obtained solutions.展开更多
In recent years, functional data has been widely used in finance, medicine, biology and other fields. The current clustering analysis can solve the problems in finite-dimensional space, but it is difficult to be direc...In recent years, functional data has been widely used in finance, medicine, biology and other fields. The current clustering analysis can solve the problems in finite-dimensional space, but it is difficult to be directly used for the clustering of functional data. In this paper, we propose a new unsupervised clustering algorithm based on adaptive weights. In the absence of initialization parameter, we use entropy-type penalty terms and fuzzy partition matrix to find the optimal number of clusters. At the same time, we introduce a measure based on adaptive weights to reflect the difference in information content between different clustering metrics. Simulation experiments show that the proposed algorithm has higher purity than some algorithms.展开更多
In order to make rail transit smart stations serve passengers better,the potential of smart stations should be optimized to reduce time cost,and relieve traffic congestion.In this paper,the construction of smart stati...In order to make rail transit smart stations serve passengers better,the potential of smart stations should be optimized to reduce time cost,and relieve traffic congestion.In this paper,the construction of smart stations based on the management experience of a subway station and the benefits of smart stations were analyzed and discussed.The construction of smart stations,as well as the key technologies for the construction of smart stations,will aid in the automation and intelligent management of subway stations.展开更多
Stem cells have shown great application potential in wound repair,tissue regeneration,and disease treatment.Therefore,a full understanding of stem cells and their related regulatory mechanisms in disease treatment is ...Stem cells have shown great application potential in wound repair,tissue regeneration,and disease treatment.Therefore,a full understanding of stem cells and their related regulatory mechanisms in disease treatment is conducive to improving the therapeutic effect of stem cells.However,thus far,there are still many unsolved mysteries in thefield of stem cells due to technical limitations,which hinder the in-depth exploration of stem cells and their wide clinical application.Single-cell sequencing(SCS)has provided very powerful and unbiased insights into cell gene expression profiles at the single-cell level,bringing exciting results to the stem cellfield.At present,SCS has been widely applied in thefield of stem cells,covering various aspects,including lineage tracing the development of stem cells,identifying new stem cell types,exploring cellular heterogeneity,and identifying internal functional subpopulations.In this paper,we focus on the latest research progress and discuss the application of SCS technology in stem cells.展开更多
DNA methylation is an important epigenetic regulatory mechanism,it regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factor(s)to DNA.In this stu...DNA methylation is an important epigenetic regulatory mechanism,it regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factor(s)to DNA.In this study,a novel methyltransferase 2a gene(Zmet2a)was cloned in maize and identified by polymerase chain reaction-base(PCR-base)using a bioinformatics strategy.The Zmet2a cDNA sequence is 2739 bp long and translates to 912 amino acid peptides.The Zmet2a protein revealed that it contains BAH and CHROMO structural domains,is a non-transmembrane protein that is hydrophilically unstable,and has no signal peptide structure.Meanwhile,we verified the biological roles of Zmet2a using transgenic Arabidopsis overexpressing Zmet2a and Zmet2a-knockout maize.Transgenic Zmet2a Arabidopsis thaliana showed highly significant advancement inflowering time,and Zmet2a-knockout maize showed advancement inflowering time,with significant changes in several traits.Altogether,these report the role of Zmet2a in the regulation offlowering time,which will lay a foundation for revealing the biological function and epigenetic regulation mechanism of Zmet2a in the growth,development andflowering of maize.展开更多
Abiotic stress reduces plant yield and quality.WRKY transcription factors play key roles in abiotic stress responses in plants,but the molecular mechanisms by which WRKY transcription factors mediate responses to drou...Abiotic stress reduces plant yield and quality.WRKY transcription factors play key roles in abiotic stress responses in plants,but the molecular mechanisms by which WRKY transcription factors mediate responses to drought and osmotic stresses in apple(Malus×domestica Borkh.)remain unclear.Here,we functionally characterized the apple GroupⅢWRKY gene MdWRKY115.qRT-PCR analysis showed that MdWRKY115 expression was up-regulated by drought and osmotic stresses.GUS activity analysis revealed that the promoter activity of MdWRKY115 was enhanced under osmotic stress.Subcellular localization and transactivation assays indicated that MdWRKY115 was localized to the nucleus and had a transcriptional activity domain at the N-terminal region.Transgenic analysis revealed that the overexpression of MdWRKY115 in Arabidopsis plants and in apple callus markedly enhanced their tolerance to drought and osmotic stresses.DNA affinity purification sequencing showed that MdWRKY115 binds to the promoter of the stress-related gene MdRD22.This binding was further verified by an electrophoretic mobility shift assay.Collectively,these findings suggest that MdWRKY115 is an important regulator of osmotic and drought stress tolerance in apple.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.32072049)Open Foundation of State Key Laboratory of Rice Biology,China(Grant No.20210208).
文摘An EMS(ethy methanesulfonate)-induced lethal etiolated(le)mutant obtained from the rice variety Zhongjian 100 was characterized by lethal etiolated phenotypes,with significantly reduced levels of chlorophyll a,chlorophyll b,total chlorophyll,and carotenoids.Additionally,the mutant displayed a significantly decreased number of chloroplast grana,along with irregular and less-stacked grana lamellae.The le mutant showed markedly diminished root length,root surface area,and root volume compared with the wild type.It also exhibited significantly lower catalase activity and total protein content,while peroxidase activity was significantly higher.Using the map-based cloning method,we successfully mapped the LE gene to a 48-kb interval between markers RM16107 and RM16110 on rice chromosome 3.A mutation(from T to C)was identified at nucleotide position 692 bp of LOC_Os03g59640(ChlD),resulting in a change from leucine to proline.By crossing HM133(a pale green mutant with a single-base substitution of A for G in exon 10 of ChlD subunit)with a heterozygous line of le(LEle),we obtained two plant lines heterozygous at both the LE and HM133 loci.Among 15 transgenic plants,3 complementation lines displayed normal leaf color with significantly higher total chlorophyll,chlorophyll a,and chlorophyll b contents.The mutation in le led to a lethal etiolated phenotype,which has not been observed in other ChlD mutants.The mutation in the AAA+domain of ChlD disrupted the interaction of ChlDle with ChlI as demonstrated by a yeast two-hybrid assay,leading to the loss of ChlD function and hindering chlorophyll synthesis and chloroplast development.Consequently,this disruption is responsible for the lethal etiolated phenotype in the mutant.
基金Supported by the National Natural Science Foundation of China, No. 30370403the Key Project of Chinese Ministry of Education, No. 705046the Doctoral Foundation of Xi’an Jiaotong University, grants No. DFXJTU2005-05
文摘AIM： TO investigate the protein profile of human hepatocarcinoma cell line SMMC-7721, to analyze the specific functions of abundant expressed proteins in the processes of hepatocarcinoma genesis, growth and metastasis, to identify the hepatocarcinoma-specific biomarkers for the early prediction in diagnosis, and to explore the new drug targets for liver cancer therapy. METHODS： Total proteins from human hepatocarcinoma cell line SMMC-7721 were separated by two-dimensional electrophoresis （2DE）. The silver-stained gel was analyzed by 2DE software Image Master 2D Elite. Interesting protein spots were identified by peptide mass fingerprinting based on matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry （MALDI-TOF-MS） and database searching. RESULTS： We obtained protein profile of human hepatocarcinoma cell line SMMC-7721. Among the twenty-one successfully identified proteins, mitofilin, endoplasmic reticulum protein ERp29, ubiquinol-cytochrome C reductase complex core protein I, peroxisomal enoyl CoA hydratase, peroxiredoxin-4 and probable 3-oxoacid CoA transferase 1 precursor were the six novel proteins identified in human hepatocarcinoma cells or tissues. Specific functions of the identified heat-shock proteins were analyzed in detail, and the results suggested that these proteins might promote tumorigenesis via inhibiting cell death induced by several cancer-related stresses or via inhibiting apoptosis at multiple points in the apoptotic signal pathway. Other identified chaperones and cancer-related proteins were also analyzed.CONCLUSION： Based on the protein profile of SMMC-7721 cells, functional analysis suggests that the identified chaperones and cancer-related proteins have their own pathways to contribute to the tumorigenesis, tumor growth and metastasis of liver cancer. Furthermore, proteomic analysis is indicated to be feasible in the cancer study.
基金supported by the China Agriculture Research System (Sweetpotato)the National High-Tech Research and Development Project of China(2011AA100607 and 2012AA101204)
文摘This paper reported firstly successful cloning of lycopene ε-cyclase （lbLCYe） gene from sweetpotato, lpomoea batatas （L.） Lam. Using rapid amplification of cDNA ends （RACE）, lbLCYe gene was cloned from sweetpotato cv. Nongdafu 14 with high carotenoid content. The 1 805 bp cDNA sequence oflbLCYe gene contained a 1236 bp open reading frame （ORF） encoding a 411 amino acids polypeptide with a molecular weight of 47 kDa and an isoelectric point （pI） of 6.95. IbLCYe protein contained one potential lycopene ε-cyclase domain and one potential FAD （flavinadenine dinucleotide）/NAD（P） （nicotinamide adenine dinucleotide phosphate）-binding domain, indicating that this protein shares the typical characteristics of LCYe proteins. The gDNA oflbLCYe gene was 4 029 bp and deduced to contain 5 introns and 6 exons. Real-time quantitative PCR analysis revealed that the expression level of IbLCYe gene was significantly higher in the storage roots of Nongdafu 14 than those in the leaves and stems. Transgenic tobacco （cv. Wisconsin 38） expressing [bLCYe gene accumulated significantly more ^-carotene compared to the untransformed control plants. These results showed that lbLCYe gene has an important function for the accumulation of carotenoids of sweetpotato.
文摘Fiber cell initiation is a complex process involving many pathways,including phytohormones and components for transcriptional and posttranscriptional regulation.Here we report expression
基金This study received financial support from the Youth Talents Special Project of Yunnan Province,“Xingdian Talents Support Program”(XDYC-QNRC-2022-0203)Southwest Forestry University Scientific Research Start-Up Funds(112116).
文摘Phenylalanine ammonia lyase(PAL)is the rate-limiting and pivotal enzyme of the general phenylpropanoid path-way,but few reports have been found on PAL genes in Pinus yunnanensis.In the present study,three PAL genes were cloned and identified from P.yunnanensis seedlings for thefirst time,namely,PyPAL-1,PyPAL-2,and PyPAL-3.Our results indicated that the open-reading frames of PyPAL genes were 2184,2157,and 2385 bp.Phylogenetic tree analysis revealed that PyPALs have high homology with other known PAL genes in other plants.In vitro enzymatic analysis showed that all three PyPAL recombinant proteins could catalyze the deamination of L-phenylalanine to form trans-cinnamic acid,but only PAL1 and PAL2 can catalyze the conversion of L-tyrosine toρ-coumaric acid.Three PyPAL genes were expressed in different tissues in 1-year-old P.yunnanensis,and such genes had different expression patterns.This study lays a foundation for further understanding of the biosynthesis of secondary metabolites in P.yunnanensis.
文摘Since entering the era of Industry 4.0,the concept of Healthcare 4.0 has also been put forward and explored by researchers.How to use Information Technology(IT)to better serve people’s healthcare is one of the most featured emerging directions in the academic circle.An important field of Healthcare 4.0 research is the reliability engineering of healthcare service.Because healthcare systems often affect the health and even life of their users,developers must be very cautious in the design,development,and operation of these healthcare systems and services.The problems to be solved include the reliability of business process,system functions,and personal healthcare data.The Functional Resonance Analysis Method(FRAM)has been applied in reliability engineering for safety-critical systems in available studies,using both qualitative and quantitative approaches.However,the method has not been applied in the field of digital healthcare services development.Therefore,to narrow the gap,we present in this paper a semi-quantitative functional resonance analysis method to develop reliable healthcare services for diabetics.Moreover,this paper has tried to improve the reliability design of the service-oriented architecture(SOA)of traditional insulin pump therapy by system thinking.
文摘Human living would be impossible without air quality. Consistent advancements in practically every aspect of contemporary human life have harmed air quality. Everyday industrial, transportation, and home activities turn up dangerous contaminants in our surroundings. This study investigated two years’ worth of air quality and outlier detection data from two Indian cities. Studies on air pollution have used numerous types of methodologies, with various gases being seen as a vector whose components include gas concentration values for each observation per-formed. We use curves to represent the monthly average of daily gas emissions in our technique. The approach, which is based on functional depth, was used to find outliers in the city of Delhi and Kolkata’s gas emissions, and the outcomes were compared to those from the traditional method. In the evaluation and comparison of these models’ performances, the functional approach model studied well.
基金This paper is one of the outcomes of the university project ( XB200349)
文摘Four samples were chosen by systematic sampling from the range-finders between 1987 and 2003 to test the range-finders’ representativeness of the scoring criteria of CET-4 compositions. The samples were analyzed from the perspectives of transitivity, modality, thematic structure and cohesion. Fisher's Exact Test from the SAS system was used to compare two-sidedly the correct rate and the constituent ratio of these functional usages between two neighboring levels. Statistical results indicate that it is the quality, not the quantity of these functional usages that distinguishes two neighboring levels. The range-finders can basically represent the abstract scoring criteria as far as the four functional systems are concerned. However, the validity of CET-4 writing needs to be improved.
基金Scientist Fund of Tangdu Hospital,Grant/Award Number:2021SHRC004Natural Science Foundation of Shaanxi Province,Grant/Award Number:2020JM‐315。
文摘Background:Systemic lupus erythematosus(SLE)is an autoimmune disease that can affect multiple systems.Sjogren's syndrome(SS)is an autoimmune disease that may be primary SS(pSS)or occur together with other autoimmune diseases,including SLE.This study aimed to explore the shared gene signatures in SLE and pSS.Methods:Gene expression data sets of SLE(GSE50772 and GSE81622)and pSS(GSE84844 and GSE48378)were obtained and analyzed for differentially expressed genes(DEGs)in peripheral blood mononuclear cells(PBMCs).A protein–protein interaction(PPI)network was constructed.Gene ontology(GO)and KEGG pathway enrichment analysis were carried out for the DEGs.Results:We screened 232 and 110 DEGs from the SLE and pSS data sets,respectively.We found 32 shared DEGs,which were all upregulated in patients compared with controls.Among these 32 DEGs,11 genes showed a more than twofold change in all data sets(IFI27,IFI44L,RSAD2,IFIT1,IFI44,USP18,IFI6,HERC5,EPSTI1,OAS1,and OAS3).PPI analysis showed that 29 genes interacted with each other.GO analysis showed that these 32 shared DEGs were mainly enriched in biological processes associated with the type Ⅰ interferon signaling pathway,defense response to viruses,response to viruses,negative regulation of viral genome replication,and the immune response.Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that these 32 DEGs were related to virus infection.Conclusion:This study showed that alterations to biological processes associated with the response to virus infection play critical roles in both SLE and pSS.
基金funded by the Natural Science Foundation of Heilongjiang Province(LH2020C045).
文摘Potentilla sericea is a heavy metal hyperaccumulator landscaping plant.MYB transcription factors play an important role in regulating plant stress response to adversity.However,there are few studies on MYB transcription factors in stress tolerance in Potentilla sericea.In this study,the PsMYB62 gene was successfully cloned from Potentilla sericea.Methods:Bioinformatic analysis and real-time quantitative PCR(qPCR)methods were used to evaluate this gene.The transgenic A.thaliana were obtained by flower dipping and the gene function was identified by determining physiological indicators under cadmium stress.Results:The open reading frame of PsMYB62 is 942 bp,which encodes 313 amino acids(aa)and belongs to the R2R3 MYB transcription factor.The plant overexpression vector PBI121-PsMYB62-GFP was constructed and successfully transferred into A.thaliana.The relative expression level of PsMYB62 was significantly increased by CdCl_(2),NaCl,ABA,and mannitol treatments.The germination rate of transgenic seeds was higher than those of wild type(WT)and empty vector(EV)under different concentrations of cadmium treatment.Upon treatment with 100μmol·L^(−1)of CdCl_(2)·2.5H_(2)O,the activities of superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)in the transgenic plants were significantly higher than those in the WT and EV.The contents of H_(2)O_(2),O_(2)·−and malondialdehyde(MDA)in transgenic lines were increased,but lower than those in WT and EV.The expression levels of AtGSH,AtPCS,and AtNAS4 that were related to the regulation of cadmium were increased,but the expression levels of transgenic lines were higher than those of WT and EV.Conclusion:The above results showed that PsMYB62 could be induced by cadmium and could improve the cadmium resistance of plants.
基金supported by grants from the National Natural Science Foundation of China(31200502,31401854)the Natural Science Foundation of Jiangsu Province(Grant No.BK20140760,BK20150552)
文摘A MADS-box gene,CiMADS9,was cloned from the male flowers of Carya illinoinensis by rapid amplification of cDNA ends. The gene was 1 077 bp with a 768 bp open reading frame encoding 255 amino acids. Multiple sequence comparisons revealed that Ci MADS9 is a typical MIKC-type MADS-box gene with a MADS-box domain and a K semi-conserved region. Phylogenetic analysis indicated that CiMADS9 belongs to the AGL15 group of the MADS-box gene family. Quantitative reverse transcription polymerase chain reaction analysis indicated that the expression levels in reproductive organs(i.e.,flowers and young fruits) were considerably higher than in vegetative tissues(i.e.,leaves and branches). The highest expression levels were observed in male flowers. An overexpression vector for CiMADS9 was constructed and the gene was inserted into the Arabidopsis thaliana genome. CiMADS9 expression was confirmed in all transgenic lines. Compared with wild-type plants,transgenic A. thaliana plants overexpressing CiMADS9 exhibited delayed flowering and an increased number of leaves.
文摘Within a framework of Halliday＇s SFL （Systemic Functional Linguistics）, this paper takes a functional approach to the analysis of the fairy tale--＂The Little Match-Seller＂ （Andersen, 1846）. The analysis is carried out in three respects： genre, register and language. Genre analysis indicates that the schematic structure of the fairy tale is very much in line with the one mentioned by Hasan. Register analysis shows that the material process is the most dominant one; the Finite in the clauses is predominantly in past tenses, and most of the Themes in the text are unmarked ones. Language analysis reveals that the lexical density in the fairy tale is relatively low. The findings in this paper can help deepen people＇s understanding of the textual features of English fairy tales.
文摘As two main approaches to analyzing literary discourses,literary criticism and stylistic analysis have been employed for a long period of time.With the application of the theories of Functional Grammar,this paper is written for the purpose of being helpful for the readers of Dylan Thomas’poem“Do not Go Gentle into That Good Night”to understand the poem’s theme better as well as appreciate the artistic adaptation of language.Furthermore,because the poem is analyzed with the application of Functional Grammar,it helps the readers to appreciate“Do not Go Gentle into That Good Night”from a new perspective.
基金Funded by the National Natural Science Foundation of China(Nos.11102136 and 41362016)the Open Project of Guangxi Key Laboratory of Disaster Prevention and Structural Safety(No.2013ZDK09)
文摘An orthotropic functionally graded piezoelectric rectangular plate with arbitrarily distributed material properties was studied, which is simply supported and grounded（electrically） on its four lateral edges. The state equations of the functionally graded piezoelectric material were obtained using the state-space approach, and a Peano-Baker series solution was obtained for the coupled electroelastic fi elds of the functionally graded piezoelectric plate subjected to mechanical and electric loading on its upper and lower surfaces. The influence of different distributions of material properties on the structural response of the plate was studied using the obtained solutions.
文摘In recent years, functional data has been widely used in finance, medicine, biology and other fields. The current clustering analysis can solve the problems in finite-dimensional space, but it is difficult to be directly used for the clustering of functional data. In this paper, we propose a new unsupervised clustering algorithm based on adaptive weights. In the absence of initialization parameter, we use entropy-type penalty terms and fuzzy partition matrix to find the optimal number of clusters. At the same time, we introduce a measure based on adaptive weights to reflect the difference in information content between different clustering metrics. Simulation experiments show that the proposed algorithm has higher purity than some algorithms.
文摘In order to make rail transit smart stations serve passengers better,the potential of smart stations should be optimized to reduce time cost,and relieve traffic congestion.In this paper,the construction of smart stations based on the management experience of a subway station and the benefits of smart stations were analyzed and discussed.The construction of smart stations,as well as the key technologies for the construction of smart stations,will aid in the automation and intelligent management of subway stations.
文摘Stem cells have shown great application potential in wound repair,tissue regeneration,and disease treatment.Therefore,a full understanding of stem cells and their related regulatory mechanisms in disease treatment is conducive to improving the therapeutic effect of stem cells.However,thus far,there are still many unsolved mysteries in thefield of stem cells due to technical limitations,which hinder the in-depth exploration of stem cells and their wide clinical application.Single-cell sequencing(SCS)has provided very powerful and unbiased insights into cell gene expression profiles at the single-cell level,bringing exciting results to the stem cellfield.At present,SCS has been widely applied in thefield of stem cells,covering various aspects,including lineage tracing the development of stem cells,identifying new stem cell types,exploring cellular heterogeneity,and identifying internal functional subpopulations.In this paper,we focus on the latest research progress and discuss the application of SCS technology in stem cells.
基金supported by Jilin Province Science and Technology Development Program(20220202014NC)the National Natural Science Foundation of China(#31471565 and#31170259).
文摘DNA methylation is an important epigenetic regulatory mechanism,it regulates gene expression by recruiting proteins involved in gene repression or by inhibiting the binding of transcription factor(s)to DNA.In this study,a novel methyltransferase 2a gene(Zmet2a)was cloned in maize and identified by polymerase chain reaction-base(PCR-base)using a bioinformatics strategy.The Zmet2a cDNA sequence is 2739 bp long and translates to 912 amino acid peptides.The Zmet2a protein revealed that it contains BAH and CHROMO structural domains,is a non-transmembrane protein that is hydrophilically unstable,and has no signal peptide structure.Meanwhile,we verified the biological roles of Zmet2a using transgenic Arabidopsis overexpressing Zmet2a and Zmet2a-knockout maize.Transgenic Zmet2a Arabidopsis thaliana showed highly significant advancement inflowering time,and Zmet2a-knockout maize showed advancement inflowering time,with significant changes in several traits.Altogether,these report the role of Zmet2a in the regulation offlowering time,which will lay a foundation for revealing the biological function and epigenetic regulation mechanism of Zmet2a in the growth,development andflowering of maize.
基金supported by grants from the Natural Science Foundation of Hebei Province(Grant No.C2022204086)the Hebei Apple Innovation Team of Modern Agricultural Industry Technology System(Grant No.HBCT2021100211)the National Natural Science Foundation of China(Grant No.32072524).
文摘Abiotic stress reduces plant yield and quality.WRKY transcription factors play key roles in abiotic stress responses in plants,but the molecular mechanisms by which WRKY transcription factors mediate responses to drought and osmotic stresses in apple(Malus×domestica Borkh.)remain unclear.Here,we functionally characterized the apple GroupⅢWRKY gene MdWRKY115.qRT-PCR analysis showed that MdWRKY115 expression was up-regulated by drought and osmotic stresses.GUS activity analysis revealed that the promoter activity of MdWRKY115 was enhanced under osmotic stress.Subcellular localization and transactivation assays indicated that MdWRKY115 was localized to the nucleus and had a transcriptional activity domain at the N-terminal region.Transgenic analysis revealed that the overexpression of MdWRKY115 in Arabidopsis plants and in apple callus markedly enhanced their tolerance to drought and osmotic stresses.DNA affinity purification sequencing showed that MdWRKY115 binds to the promoter of the stress-related gene MdRD22.This binding was further verified by an electrophoretic mobility shift assay.Collectively,these findings suggest that MdWRKY115 is an important regulator of osmotic and drought stress tolerance in apple.
