Fusarium head blight(FHB),mainly caused by the fungal pathogen Fusarium graminearum,is one of the most destructive wheat diseases.Besides directly affecting the yield,the mycotoxin residing in the kernel greatly threa...Fusarium head blight(FHB),mainly caused by the fungal pathogen Fusarium graminearum,is one of the most destructive wheat diseases.Besides directly affecting the yield,the mycotoxin residing in the kernel greatly threatens the health of humans and livestock.Xinong 979(XN979)is a widely cultivated wheat elite with high yield and FHB resistance.However,its resistance mechanism remains unclear.In this study,we studied the expression of genes involved in plant defense in XN979 by comparative transcriptomics.We found that the FHB resistance in XN979 consists of two lines of defense.The first line of defense,which is constitutive,is knitted via the enhanced basal expression of lignin and jasmonic acid(JA)biosynthesis genes.The second line of defense,which is induced upon F.graminearum infection,is contributed by the limited suppression of photosynthesis and the struggle of biotic stress-responding genes.Meanwhile,the effective defense in XN979 leads to an inhibition of fungal gene expression,especially in the early infection stage.The formation of the FHB resistance in XN979 may coincide with the breeding strategies,such as selecting high grain yield and lodging resistance traits.This study will facilitate our understanding of wheat-F.graminearum interaction and is insightful for breeding FHB-resistant wheat.展开更多
Fusarium head blight(FHB)is one of the most detrimental wheat diseases which greatly decreases the yield and grain quality,especially in the middle and lower reaches of the Yangtze River of China.Fhb1 and Fhb2 are two...Fusarium head blight(FHB)is one of the most detrimental wheat diseases which greatly decreases the yield and grain quality,especially in the middle and lower reaches of the Yangtze River of China.Fhb1 and Fhb2 are two major resistance loci against Fusarium graminearum.Yangmai 15(YM15)is one of the most popular varieties in the middle and lower reaches of the Yangtze River,and it has good weak gluten characters but poor resistance to FHB.Here we used Fhb1 and Fhb2 to improve the FHB resistance of YM15 by a molecular marker-assisted selection(MAS)backcrossing strategy.The selection of agronomic traits was performed for each generation.We successfully selected seven introgressed lines which carry homozygous Fhb1 and Fhb2 with significantly higher FHB resistance than the recurrent parent YM15.Three of the introgressed lines had agronomic and quality characters that were similar to YM15.This study demonstrates that the pyramiding of Fhb1 and Fhb2 could significantly improve the FHB resistance in wheat using the MAS approach.展开更多
Fhb7 is a major gene that was transferred from Thinopyrum ponticum to chromosome 7D of wheat(Triticum aestivum)and confers resistance to both Fusarium head blight(FHB)and Fusarium crown rot(FCR).However,Fhb7 is tightl...Fhb7 is a major gene that was transferred from Thinopyrum ponticum to chromosome 7D of wheat(Triticum aestivum)and confers resistance to both Fusarium head blight(FHB)and Fusarium crown rot(FCR).However,Fhb7 is tightly linked to the PSY-E2 gene,which causes yellow flour,limiting its application in breeding.To break this linkage,marker K-PSY was developed for tagging PSY-E2 and used with Fhb7 markers to identify recombination between the two genes.Screening 21,000 BC1F2 backcross progeny(Chinese Spring ph1bph1b*2/SDAU 2028)revealed two Fhb7^(+)wheat-Tp7el_(2)L lines,Shannong 2–16and Shannong 16–1,that carry a desired truncated Fhb7^(+)translocation segment without PSY-E2.The two lines show levels of resistance to FHB and FCR similar to those of the original translocation line SDAU 2028,but have white flour.To facilitate Fhb7 use in wheat breeding,STS markers were developed and used to isolate Fhb7 on a truncated Tp7el_(2) translocation segment.Near-isogenic lines carrying the Fhb7^(+)segment were generated in the backgrounds of three commercial cultivars,and Fhb7^(+)lines showed increased FHB and FCR resistance without yield penalty.The breakage of the tight linkage between Fhb7 and PSY-E2 via homoeologous recombination provides genetic resources for improvement of wheat resistance to FHB and FCR and permit the large-scale deployment of Fhb7 in breeding using marker-assisted selection.展开更多
This report reviews the characteristics of JS399-19, a novel cyanoacrylate fungicide. JS399-19 strongly inhibits the mycelial growth of the fungal plant pathogens of the genus Fusarium and exhibits great potential in ...This report reviews the characteristics of JS399-19, a novel cyanoacrylate fungicide. JS399-19 strongly inhibits the mycelial growth of the fungal plant pathogens of the genus Fusarium and exhibits great potential in controlling Fusarium head blight （FHB） on wheat and other cereals. The mode of action of JS399-19 is evidently different from that of benzimidazole （for example, carbendazim） and other sort of fungicides, making it a possible replacement for carbendazim in China to manage carbendazim-resistant subpopulations of Fusarium graminearum and F. asiaticum. JS399-t9 has excellent protective and curative activity against these pathogens. Incorrect use of this fungicide, however, is likely to select for resistance. Among JS399-19-resistant mutants of F. asiaticum induced in the laboratory, the resistant level of mutants was high and the phenotype of resistance against JS399-19 was conferred by a major gene by genetic analysis. The fitness of laboratory-induced JS399-19-resistant mutants of F. asiaticum was nearly equal to that of their parents. JS399-19 lacks cross resistance with other sort fungicides. To control FHB with JS399-19 and to delay the development of the fungicide-resistance, farmers should use tank mixtures containing JS399-19 and carbendazim, metconazole, tebuconazole, or prothioconazole.展开更多
Fusarium head blight(FHB) is a global wheat disease that devastates wheat production. Resistance to FHB spread within a wheat spike(type Ⅱ resistance) and to mycotoxin accumulation in infected kernel(type Ⅲ resistan...Fusarium head blight(FHB) is a global wheat disease that devastates wheat production. Resistance to FHB spread within a wheat spike(type Ⅱ resistance) and to mycotoxin accumulation in infected kernel(type Ⅲ resistance) are the two main types of resistance. Of hundreds of QTL that have been reported, only a few can be used in wheat breeding because most show minor and/or inconsistent effects in different genetic backgrounds. We describe a new strategy for identifying robust and reliable meta-QTL(mQTL)that can be used for improvement of wheat FHB resistance. It involves integration of mQTL analysis with mQTL physical mapping and identification of single-copy markers and candidate genes. Using metaanalysis, we consolidated 625 original QTL from 113 publications into 118 genetic map-based mQTL(gmQTL). These gmQTL were further located on the Chinese Spring reference sequence map. Finally, 77 high-confidence mQTL(hcmQTL) were selected from the reference sequence-based mQTL(smQTL).Locus-specific single nucleotide polymorphism(SNP) and simple sequence repeat(SSR) markers and17 genes responsive to FHB were then identified in the hcmQTL intervals by combined analysis of transcriptomic and proteomic data. This work may lead to a comprehensive molecular breeding platform for improving wheat resistance to FHB.展开更多
Fusarium head blight(FHB)is a worldwide devastating disease of small grain cereals and Fusarium graminearum species complex(FGSC)is the major pathogen causing the disease.The epidemics of FHB lead to the reduction of ...Fusarium head blight(FHB)is a worldwide devastating disease of small grain cereals and Fusarium graminearum species complex(FGSC)is the major pathogen causing the disease.The epidemics of FHB lead to the reduction of grain yield and economic losses.Additionally,mycotoxins produced by the FHB pathogens are hazardous to the health of human and livestock.In this review,we summarize the epidemiology of FHB,and introduce effects of this disease on economy,environment and food safety.We focus on the integrated management approaches for controlling FHB including agronomic practices,resistant cultivars,chemical control,and biocontrol.In addition,we also discuss the potential novel management strategies against FHB and mycotoxin.展开更多
A few EST-derived STS markers localized on Qfhs.ndsu-3BS, a major QTL for resistance to Fusarium head blight （FHB） in wheat, have been previously identified in the ＇Sumai 3＇/＇Stoa＇ population. In this study, we ...A few EST-derived STS markers localized on Qfhs.ndsu-3BS, a major QTL for resistance to Fusarium head blight （FHB） in wheat, have been previously identified in the ＇Sumai 3＇/＇Stoa＇ population. In this study, we used a ＇Wangshuibai＇ （resistant）/＇Seri82＇ （susceptible） derived population, linkage group, QTL, and quantitative gene expression analysis to assess the genetic background dependence and stability of the EST-derived STS markers for use in marker aided selection to improve FHB resistance in wheat. Based on our results, a QTL in the map interval of Xsts3B-138_1-Xgwrn493 on chromosome 3BS was detected for FHB resistance, which accounted for up to 16% of the phenotypic variation. BLASTN analysis indicated that Xsts3B-138_1 sequence had significant similarity with the resistance gene analogue. Real-time quantitative PCR showed that the relative expression of Xsts3B-138_1 in ＇Wangshuibai＇ at 96 h after inoculation was 2.6 times higher than ＇Seri82＇. Our results underlined that EST-derived STS3B-138 markers could be predominantly used in marker aided selection to improve FHB resistance in wheat.展开更多
Fusarium head blight （FHB） is a destructive disease widespread in warm humid and semi-humid areas, which not only causes yield and grain quality losses, but also produces myeatoxin deoxynivalenol, thus posing a seri...Fusarium head blight （FHB） is a destructive disease widespread in warm humid and semi-humid areas, which not only causes yield and grain quality losses, but also produces myeatoxin deoxynivalenol, thus posing a serious threat to wheat production in the world. In this study, 15 introgression lines with signifi- cantly different levels of resistance to FHB were screened as experimental materials to detect the intmgressed fragments by SSR markers from BC4F5 progenies of Atrd/Laizhou 953. The results showed that the number of polymorphic markers detected on each chromosome varied greatly, and polymorphic markers detected on chromosomes 5D and 5A were more than on other chromosomes. Am3 fragment could be detected in 15 introgression lines with 38 pairs of primers; the number of detected introgressed fragments varied among different introgression lines. Among 21 linkage groups, no introgressed fragments were detected on chromosomes 1A, 6D and 7A; the largest number of intregressed fragments was detected on chromosomes 3D and 5B. In 15 intregression lines, three QTLs for plant height, spike length and disease index detected. Qdi-caas-5A, which was derived from Am3, might be related with Type III resistance.展开更多
Bread wheat(Triticum aestiuum L.),which provides about 20%of daily calorie intake,is the most widely cultivated crop in the world,in terms of total area devoted to its cultivation.Therefore,even small increases in whe...Bread wheat(Triticum aestiuum L.),which provides about 20%of daily calorie intake,is the most widely cultivated crop in the world,in terms of total area devoted to its cultivation.Therefore,even small increases in wheat yield can translate into large gains.Reducing the gap between actual and potential grain yield in wheat is a crucial task to feed the increasing world population.Fusarium head blight(FHB)caused by the pathogenic fungus Fusaium graminearum and related Fusarium species is one of the most devastating wheat diseases throughout the world.This disease reduces not only the yield but also the quality by contaminating the grain with mycotoxins harmful for humans,animals and the environment.In recent years,remarkable achievements attained in omics"technologies have not only provided new insights into understanding of processes involved in pathogenesis but also helped develop effective new tools for practical plant breeding.Sequencing of the genomes of various wheat patho gens,including F.graminearum,as well as those of bread and durum wheat and their wild relatives,together with advances made in transcriptomics and bioinformatics,has allowed the identification of candidate pathogen effectors and corresponding host resistance(R)and susceptibility(S)genes.However,so far,FHB effectors and wheat susceptibility genes/factors have been poorly studied.In this paper,we first briefly highlighted recent examples of improving resistance against pathogens via new techniques in different host species.We then propose effective strategies towards developing wheat cultivars with improved resistance to FHB.We hope that the article will spur discussions and interest among researchers about novel approaches with great potential for improving wheat against FHB.展开更多
Fusarium head blight is one of the most important diseases affecting wheat yield and quality.It is of great significance to carry out intelligent monitoring of wheat Fusarium head blight for high yield,high quality an...Fusarium head blight is one of the most important diseases affecting wheat yield and quality.It is of great significance to carry out intelligent monitoring of wheat Fusarium head blight for high yield,high quality and sustainable development of wheat.On the basis of identifying the harms of wheat Fusarium head blight,this paper analyzed the monitoring technology of wheat Fusarium head blight based on satellite remote sensing,hyperspectral,near-infrared,Internet of things and photoelectric system,to provide a reference for the intelligent monitoring of wheat Fusarium head blight.展开更多
The mutation of the gene encoding a stearoyl-acyl carrier protein fatty acid desaturase（ssi2） has been proved to enhance pathogen resistance in several plants, while it＇s potential to regulate biotic and abiotic st...The mutation of the gene encoding a stearoyl-acyl carrier protein fatty acid desaturase（ssi2） has been proved to enhance pathogen resistance in several plants, while it＇s potential to regulate biotic and abiotic stresses in wheat is still unclear. In this study, we cloned TaSSI2 gene in wheat and provided several evidences of its involvement in multiple biological functions. By using barley stripe mosaic virus（BSMV）-induced gene silencing（VIGS） in wheat, it was found that TaSSI2 negatively regulated both powdery mildew and Fusarium head blight（FHB） resistance, which was consistent with the phenotype observed in knock-out mutants of Kronos. The expression of TaSSI2 was down-regulated by in vitro treatments of methyl jasmonate（Me JA）, but positively regulated by salicylic acid（SA） and abscisic acid（ABA）, implying the cross-talk between different hormone signaling pathways involved in wheat to regulate biotic stresses is still to be elucidated. Furthermore, the up-regulated expression of PR4 and PR5 indicated that TaSSI2 probably regulated FHB resistance by depressing the SA signaling pathway in wheat. In addition, the over-expression of TaSSI2 increased the content of linolenic acid（18：3） and subsequently enhanced drought tolerance of transgenic Brachypodium. This phenomenon might be associated with its subcellular localization in the whole cytosol, partly overlapping with Golgi apparatus and the secreted vesicles. As a stearoyl-acyl carrier protein fatty acid desaturase, TaSSI2 was proposed to be involved in cell lipid metabolism and carried targets out of the cell from membrane or wax synthesis, resulting in enhanced drought tolerance in plant.展开更多
Fusarium head blight(FHB) or scab caused by Fusarium graminearum is a major threat to wheat production in China as well as in the world. To combat this disease, multiple efforts have been carried out internationally. ...Fusarium head blight(FHB) or scab caused by Fusarium graminearum is a major threat to wheat production in China as well as in the world. To combat this disease, multiple efforts have been carried out internationally. In this article, we review our long-time effort in identifying the resistance genes and dissecting the resistance mechanisms by both forward and reverse genetics approaches in the last two decades. We present recent progress in resistance QTL identification, candidate functional gene discovery, marker-assisted improvement of FHB resistant varieties, and findings in investigating association of signal molecules, such as Ca^(++),SA, JA, and ET, with FHB response, with the assistance from rapidly growing genomics platforms. The information will be helpful for designing novel and efficient approaches to curb FHB.展开更多
The objective of this paper is to review progress made in wheat breeding for Fusarium head blight(FHB) resistance in China, the United States of America(USA), and Canada. In China,numerous Chinese landraces possessing...The objective of this paper is to review progress made in wheat breeding for Fusarium head blight(FHB) resistance in China, the United States of America(USA), and Canada. In China,numerous Chinese landraces possessing high levels of FHB resistance were grown before the 1950 s. Later, pyramiding multiple sources of FHB resistance from introduced germplasm such as Mentana and Funo and locally adapted cultivars played a key role in combining satisfactory FHB resistance and high yield potential in commercial cultivars.Sumai 3, a Chinese spring wheat cultivar, became a major source of FHB resistance in the USA and Canada, and contributed to the release of more than 20 modern cultivars used for wheat production, including the leading hard spring wheat cultivars Alsen, Glenn, Barlow and SY Ingmar from North Dakota, Faller and Prosper from Minnesota, and AAC Brandon from Canada. Brazilian wheat cultivar Frontana, T. dicoccoides and other local germplasm provided additional sources of resistance. The FHB resistant cultivars mostly relied on stepwise accumulation of favorable alleles of both genes for FHB resistance and high yield,with marker-assisted selection being a valuable complement to phenotypic selection. With the Chinese Spring reference genome decoded and resistance gene Fhb1 now cloned, new genomic tools such as genomic selection and gene editing will be available to breeders, thus opening new possibilities for development of FHB resistant cultivars.展开更多
The gene Fhb1 has been used in many countries to improve wheat Fusarium head blight(FHB) resistance. To make better use of this gene in the Yellow-Huai River Valleys Winter Wheat Zone(YHWZ), the most important wheat-p...The gene Fhb1 has been used in many countries to improve wheat Fusarium head blight(FHB) resistance. To make better use of this gene in the Yellow-Huai River Valleys Winter Wheat Zone(YHWZ), the most important wheat-producing region of China, it is desirable to elucidate its effects on FHB resistance and agronomic traits in different genetic backgrounds. Based on a diagnostic marker for Fhb1, six BC2 populations were developed by crossing dwarf-male-sterile(DMS)-Zhoumai 16 to three Fhb1 donors(Ningmai 9, Ningmai 13, and Jianyang 84) and backcrossing to Zhoumai 16 and Zhoumai16’s derivative cultivars(Lunxuan 136 and Lunxuan 13) using marker-assisted backcross breeding. The progenies were assessed for FHB resistance and major agronomic traits.The Fhb1 alleles were identified using the gene-specific molecular marker. The plants with the Fhb1-resistant genotype(Fhb1-R) in these populations showed significantly fewer infected spikelets than those with the Fhb1-susceptible genotype(Fhb1-S). When Lunxuan 136 was used as the recurrent parent, Fhb1-R plants showed significantly fewer infected spikelets per spike than Fhb1-R plants produced using Lunxuan 13 as the recurrent parent, indicating that the genetic backgrounds of Fhb1 influence the expression of FHB resistance. Fhb1-R plants from the DMS-Zhoumai 16/Ningmai 9//Zhoumai 16/3/Lunxuan 136 population showed the highest FHB resistance among the six populations and a significantly higher level of FHB resistance than the moderately susceptible control Huaimai 20. No significant phenotypic differences between Fhb1-R and Fhb1-S plants were observed for the eight agronomic traits investigated. These results suggest that it is feasible to improve FHB resistance of winter wheat withoutreducing yield potential by introgressing Fhb1 resistance allele into FHB-susceptible cultivars in the YHWZ.展开更多
Fusarium head blight (FHB) is a worldwide disease for wheat or barley. The contamination of important agricultural products with the trichothecene mycotoxins such as deoxynivalenol (DON) or 4,15-diacetoxyscirpe-
Fusarium head blight (FHB) is a worldwide destructive disease of wheat in the warm, semi-humid or humid regions, especially serious in China. The disease not only causes significant losses in yield and reduces grain q...Fusarium head blight (FHB) is a worldwide destructive disease of wheat in the warm, semi-humid or humid regions, especially serious in China. The disease not only causes significant losses in yield and reduces grain quanlity, but also induces toxin to contaminated seeds, which is harmful to the healthy of human and livestocks, So it is important to control it. There are several methods to control Fusarium head blight (FHB). Such as using Crop rotation, Soil cultivation and Fertiliser, biological control, Fungicides control, transgenes, resistance to control Fusarium head blight (FHB). All of these methods gain some effect, but also exist their deficiency. Sometimes crop rotation had no significant effect on DON contamination of wheat grain, subsequent reductions in DON contamination were inconsistent when using Soil cultivation and Fertiliser, Unfortunately, under field conditions, the biological control achieved has been shown to be variable and in some tests has failed to give any control, The use of fungicides, however, have not been consistently effective in controlling FHB and in reducing DON formation, transgene-silencing at different generations is a problem to use transgenes, Information on location of QTL for FHB resistance should improve dramatically in the near future on resistance to control Fusarium head blight (FHB). Therefore, it is pressing to improve control methods, especially to DON.展开更多
Fusarium head blight, one of the most destructive diseases of wheat (Triticum aestivum L.), results in significant economic losses from reduced grain yield and quality. In recent decades, the disease has been frequent...Fusarium head blight, one of the most destructive diseases of wheat (Triticum aestivum L.), results in significant economic losses from reduced grain yield and quality. In recent decades, the disease has been frequently recorded, especially under warm and wet climatic conditions. Genetic resistance has engaged plant breeders because the use of resistant cultivars is the most economical, effective, and environmentally friendly method of control. In the present paper, we summarize the research on resistance genetics of Fusarium head blight, suggest a new method for evaluating Fusarium head blight resistance, and recommend strategies for creating and developing new sources of resistance to Fusarium head blight through the use of alien genes and chromosomal segments.展开更多
Fusarium head blight (FHB) is one of the most destructive diseases in global wheat production. In order to count the FHB-infected wheat ears under field conditions, this study proposed an algorithm for diseased wheat ...Fusarium head blight (FHB) is one of the most destructive diseases in global wheat production. In order to count the FHB-infected wheat ears under field conditions, this study proposed an algorithm for diseased wheat ear detection based on improved YOLOv5s (Tr-YOLOv5s). The Swin Transformer was used to replace the CSPDarknet backbone network to enhance the extraction of characteristic information of the population wheat ears of FHB in the field background. The convolutional block attention module (CBAM) attention mechanism was added to improve the detection effect of target wheat ears, subsequently improving the overall accuracy of the model. The original loss function complete intersection over union (CIoU) was replaced by Scylla intersection over union (SIoU) loss to accelerate the model convergence and decrease the loss value. The results showed that the mean average precision (mAP) of the Tr-YOLOv5s model reached 90.64%, making a 4.63% improvement compared to the original YOLOv5s model. The improved model could quickly detect and count wheat FHB ear in the field environment, which laid a foundation for the subsequent automatic disease identification and grading of wheat FHB under field conditions.展开更多
Thinopyrum elongatum （2n = 2x = 14, EE）, a wild relative of wheat, has been suggested as a potentially novel source of resistance to several major wheat diseases including Fusarium Head Blight （FHB）. In this study...Thinopyrum elongatum （2n = 2x = 14, EE）, a wild relative of wheat, has been suggested as a potentially novel source of resistance to several major wheat diseases including Fusarium Head Blight （FHB）. In this study, a series of wheat （cv. Chinese Spring, CS） substitution and ditelosomic lines, including Th. elongatum additions, were assessed for Type II resistance to FHB. Results indicated that the lines containing chromosome 7E of Th. elongatum gave a high level of resistance to FHB, wherein the infection did not spread beyond the inoculated floret. Furthermore, it was determined that the novel resistance gene（s） of 7E was located on the short-ann （7ES） based on sharp difference in FHB resistance between the two 7E ditelosomic lines for each arm. On the other hand, Th. elongatum chromosomes 5E and 6E likely contain gene（s） for susceptibility to FHB because the disease spreads rapidly within the inoculated spikes of these lines. Genomic in situ hybridization （GISH） analysis revealed that the alien chromosomes in the addition and substitution lines were intact, and the lines did not contain discernible genomic aberrations. GISH and multicolor-GISH analyses were further performed on three trans- location lines that also showed high levels of resistance to FHB. Lines TA3499 and TA3695 were shown to contain one pair of wheat-Th. elongatum translocated chromosomes involving fragments of 7D plus a segment of the 7E, while line TA3493 was found to contain one pair of wheat-Th, elongatum translocated chromosomes involving the D- and A-genome chromosomes of wheat. Thus, this study has established that the short-arm of chromosome 7E of Th. elongatum harbors gene（s） highly resistant to the spreading of FHB, and chromatin of 7E introgressed into wheat chromosomes largely retained the resistance, implicating the feasibility of using these lines as novel material for breeding FHB-resistant wheat cultivars.展开更多
Thaumatin-like proteins (TLPs) play potential roles in plant resistance to various diseases. Identifying TLPs is neces-sary to determine their function and apply them to plant disease resistance. However, limited info...Thaumatin-like proteins (TLPs) play potential roles in plant resistance to various diseases. Identifying TLPs is neces-sary to determine their function and apply them to plant disease resistance. However, limited information is available about TLP-family genes in wheat, especially regarding their responses to Fusarium species, which cause Fusarium head blight in wheat. In this study, we conducted a comprehensive genome-wide survey of TLP genes in wheat and identified 129 TLP genes in the wheat genome, which were unevenly distributed on 21 wheat chromosomes, with 5A containing the highest number. Phylogenetic analysis showed that these 129 wheat TLP genes together with 24 Arabidopsis TLPs were classified into 7 groups based on the protein sequences. We systematically analyzed the genes in terms of their sequence characterization, chromosomal locations, exon-intron distribution, duplication (tandem and segmental) events and expression profiles in response to Fusarium infection. Furthermore, we analyzed differen-tially expressed TLP genes based on publicly available RNA-seq data obtained from a resistant near isogenic wheat line at different time points after Fusarium graminearum inoculation. Then, the expression of 9 differentially expressed TLP genes was confirmed by real-time PCR, and these 9 genes were all upregulated in the resistant Sumai 3 variety, which was generally consistent with the RNA-seq data. Our results provide a basis for selecting candidate wheat TLP genes for further studies to determine the biological functions of the TLP genes in wheat.展开更多
基金This work was supported by the grants from the National Key R&D Program of China(2022YFD1400100)the National Natural Science Foundation of China(32072505 and 31701747)+1 种基金the Chinese Universities Scientific Fund(2452020222)the National Innovation and Entrepreneurship Training Program for College Students China(202110712255)。
文摘Fusarium head blight(FHB),mainly caused by the fungal pathogen Fusarium graminearum,is one of the most destructive wheat diseases.Besides directly affecting the yield,the mycotoxin residing in the kernel greatly threatens the health of humans and livestock.Xinong 979(XN979)is a widely cultivated wheat elite with high yield and FHB resistance.However,its resistance mechanism remains unclear.In this study,we studied the expression of genes involved in plant defense in XN979 by comparative transcriptomics.We found that the FHB resistance in XN979 consists of two lines of defense.The first line of defense,which is constitutive,is knitted via the enhanced basal expression of lignin and jasmonic acid(JA)biosynthesis genes.The second line of defense,which is induced upon F.graminearum infection,is contributed by the limited suppression of photosynthesis and the struggle of biotic stress-responding genes.Meanwhile,the effective defense in XN979 leads to an inhibition of fungal gene expression,especially in the early infection stage.The formation of the FHB resistance in XN979 may coincide with the breeding strategies,such as selecting high grain yield and lodging resistance traits.This study will facilitate our understanding of wheat-F.graminearum interaction and is insightful for breeding FHB-resistant wheat.
基金supported by the National Natural Science Foundation of China(31901544 and 2071999)the National Key Research and Development Program of China(2017YFD0100801)。
文摘Fusarium head blight(FHB)is one of the most detrimental wheat diseases which greatly decreases the yield and grain quality,especially in the middle and lower reaches of the Yangtze River of China.Fhb1 and Fhb2 are two major resistance loci against Fusarium graminearum.Yangmai 15(YM15)is one of the most popular varieties in the middle and lower reaches of the Yangtze River,and it has good weak gluten characters but poor resistance to FHB.Here we used Fhb1 and Fhb2 to improve the FHB resistance of YM15 by a molecular marker-assisted selection(MAS)backcrossing strategy.The selection of agronomic traits was performed for each generation.We successfully selected seven introgressed lines which carry homozygous Fhb1 and Fhb2 with significantly higher FHB resistance than the recurrent parent YM15.Three of the introgressed lines had agronomic and quality characters that were similar to YM15.This study demonstrates that the pyramiding of Fhb1 and Fhb2 could significantly improve the FHB resistance in wheat using the MAS approach.
基金supported by the National Natural Science Foundation of China(32030081,31871610)the Agricultural Variety Improvement Project of Shandong Province(2019LZGC016)the U.S.Wheat and Barley Scab Initiative。
文摘Fhb7 is a major gene that was transferred from Thinopyrum ponticum to chromosome 7D of wheat(Triticum aestivum)and confers resistance to both Fusarium head blight(FHB)and Fusarium crown rot(FCR).However,Fhb7 is tightly linked to the PSY-E2 gene,which causes yellow flour,limiting its application in breeding.To break this linkage,marker K-PSY was developed for tagging PSY-E2 and used with Fhb7 markers to identify recombination between the two genes.Screening 21,000 BC1F2 backcross progeny(Chinese Spring ph1bph1b*2/SDAU 2028)revealed two Fhb7^(+)wheat-Tp7el_(2)L lines,Shannong 2–16and Shannong 16–1,that carry a desired truncated Fhb7^(+)translocation segment without PSY-E2.The two lines show levels of resistance to FHB and FCR similar to those of the original translocation line SDAU 2028,but have white flour.To facilitate Fhb7 use in wheat breeding,STS markers were developed and used to isolate Fhb7 on a truncated Tp7el_(2) translocation segment.Near-isogenic lines carrying the Fhb7^(+)segment were generated in the backgrounds of three commercial cultivars,and Fhb7^(+)lines showed increased FHB and FCR resistance without yield penalty.The breakage of the tight linkage between Fhb7 and PSY-E2 via homoeologous recombination provides genetic resources for improvement of wheat resistance to FHB and FCR and permit the large-scale deployment of Fhb7 in breeding using marker-assisted selection.
基金sponsored by the National Natural Science Foundation of China (30971891)the Natural Science Foundation of Jiangsu Province, China(BK2008337)the Anhui Provincial Natural Sci-ence Foundation,China (10040606Q26)
文摘This report reviews the characteristics of JS399-19, a novel cyanoacrylate fungicide. JS399-19 strongly inhibits the mycelial growth of the fungal plant pathogens of the genus Fusarium and exhibits great potential in controlling Fusarium head blight （FHB） on wheat and other cereals. The mode of action of JS399-19 is evidently different from that of benzimidazole （for example, carbendazim） and other sort of fungicides, making it a possible replacement for carbendazim in China to manage carbendazim-resistant subpopulations of Fusarium graminearum and F. asiaticum. JS399-t9 has excellent protective and curative activity against these pathogens. Incorrect use of this fungicide, however, is likely to select for resistance. Among JS399-19-resistant mutants of F. asiaticum induced in the laboratory, the resistant level of mutants was high and the phenotype of resistance against JS399-19 was conferred by a major gene by genetic analysis. The fitness of laboratory-induced JS399-19-resistant mutants of F. asiaticum was nearly equal to that of their parents. JS399-19 lacks cross resistance with other sort fungicides. To control FHB with JS399-19 and to delay the development of the fungicide-resistance, farmers should use tank mixtures containing JS399-19 and carbendazim, metconazole, tebuconazole, or prothioconazole.
基金supported by the National Key R&D Program,Intergovernmental Key Items for International Scientific and Technological Innovation Cooperation(2018YFE0107700)the National Natural Science Foundation of China(31771772)+2 种基金the Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX19_2109)the National Key R&D Program for Breeding of Top-seven Crops(2017YFD0100801)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘Fusarium head blight(FHB) is a global wheat disease that devastates wheat production. Resistance to FHB spread within a wheat spike(type Ⅱ resistance) and to mycotoxin accumulation in infected kernel(type Ⅲ resistance) are the two main types of resistance. Of hundreds of QTL that have been reported, only a few can be used in wheat breeding because most show minor and/or inconsistent effects in different genetic backgrounds. We describe a new strategy for identifying robust and reliable meta-QTL(mQTL)that can be used for improvement of wheat FHB resistance. It involves integration of mQTL analysis with mQTL physical mapping and identification of single-copy markers and candidate genes. Using metaanalysis, we consolidated 625 original QTL from 113 publications into 118 genetic map-based mQTL(gmQTL). These gmQTL were further located on the Chinese Spring reference sequence map. Finally, 77 high-confidence mQTL(hcmQTL) were selected from the reference sequence-based mQTL(smQTL).Locus-specific single nucleotide polymorphism(SNP) and simple sequence repeat(SSR) markers and17 genes responsive to FHB were then identified in the hcmQTL intervals by combined analysis of transcriptomic and proteomic data. This work may lead to a comprehensive molecular breeding platform for improving wheat resistance to FHB.
基金the Science and Technology Project of Zhejiang Province,China(2018C02G2011110)the National Natural Science Foundation of China(31930088 and 32001855)the earmarked fund for China Agriculture Research System(CARS-3-1-29).
文摘Fusarium head blight(FHB)is a worldwide devastating disease of small grain cereals and Fusarium graminearum species complex(FGSC)is the major pathogen causing the disease.The epidemics of FHB lead to the reduction of grain yield and economic losses.Additionally,mycotoxins produced by the FHB pathogens are hazardous to the health of human and livestock.In this review,we summarize the epidemiology of FHB,and introduce effects of this disease on economy,environment and food safety.We focus on the integrated management approaches for controlling FHB including agronomic practices,resistant cultivars,chemical control,and biocontrol.In addition,we also discuss the potential novel management strategies against FHB and mycotoxin.
基金Agricultural Biotechnology Research Institute of Iran (Iranian Ministry of Ji-had-e-Agriculture)
文摘A few EST-derived STS markers localized on Qfhs.ndsu-3BS, a major QTL for resistance to Fusarium head blight （FHB） in wheat, have been previously identified in the ＇Sumai 3＇/＇Stoa＇ population. In this study, we used a ＇Wangshuibai＇ （resistant）/＇Seri82＇ （susceptible） derived population, linkage group, QTL, and quantitative gene expression analysis to assess the genetic background dependence and stability of the EST-derived STS markers for use in marker aided selection to improve FHB resistance in wheat. Based on our results, a QTL in the map interval of Xsts3B-138_1-Xgwrn493 on chromosome 3BS was detected for FHB resistance, which accounted for up to 16% of the phenotypic variation. BLASTN analysis indicated that Xsts3B-138_1 sequence had significant similarity with the resistance gene analogue. Real-time quantitative PCR showed that the relative expression of Xsts3B-138_1 in ＇Wangshuibai＇ at 96 h after inoculation was 2.6 times higher than ＇Seri82＇. Our results underlined that EST-derived STS3B-138 markers could be predominantly used in marker aided selection to improve FHB resistance in wheat.
基金Supported by Crop Science Advantage Discipline of Jiangsu Province
文摘Fusarium head blight （FHB） is a destructive disease widespread in warm humid and semi-humid areas, which not only causes yield and grain quality losses, but also produces myeatoxin deoxynivalenol, thus posing a serious threat to wheat production in the world. In this study, 15 introgression lines with signifi- cantly different levels of resistance to FHB were screened as experimental materials to detect the intmgressed fragments by SSR markers from BC4F5 progenies of Atrd/Laizhou 953. The results showed that the number of polymorphic markers detected on each chromosome varied greatly, and polymorphic markers detected on chromosomes 5D and 5A were more than on other chromosomes. Am3 fragment could be detected in 15 introgression lines with 38 pairs of primers; the number of detected introgressed fragments varied among different introgression lines. Among 21 linkage groups, no introgressed fragments were detected on chromosomes 1A, 6D and 7A; the largest number of intregressed fragments was detected on chromosomes 3D and 5B. In 15 intregression lines, three QTLs for plant height, spike length and disease index detected. Qdi-caas-5A, which was derived from Am3, might be related with Type III resistance.
基金funded by the Research Council of Lithuania,grant No.DOTSUT-218(01.2.2-LMT-K-718-01-0065)。
文摘Bread wheat(Triticum aestiuum L.),which provides about 20%of daily calorie intake,is the most widely cultivated crop in the world,in terms of total area devoted to its cultivation.Therefore,even small increases in wheat yield can translate into large gains.Reducing the gap between actual and potential grain yield in wheat is a crucial task to feed the increasing world population.Fusarium head blight(FHB)caused by the pathogenic fungus Fusaium graminearum and related Fusarium species is one of the most devastating wheat diseases throughout the world.This disease reduces not only the yield but also the quality by contaminating the grain with mycotoxins harmful for humans,animals and the environment.In recent years,remarkable achievements attained in omics"technologies have not only provided new insights into understanding of processes involved in pathogenesis but also helped develop effective new tools for practical plant breeding.Sequencing of the genomes of various wheat patho gens,including F.graminearum,as well as those of bread and durum wheat and their wild relatives,together with advances made in transcriptomics and bioinformatics,has allowed the identification of candidate pathogen effectors and corresponding host resistance(R)and susceptibility(S)genes.However,so far,FHB effectors and wheat susceptibility genes/factors have been poorly studied.In this paper,we first briefly highlighted recent examples of improving resistance against pathogens via new techniques in different host species.We then propose effective strategies towards developing wheat cultivars with improved resistance to FHB.We hope that the article will spur discussions and interest among researchers about novel approaches with great potential for improving wheat against FHB.
文摘Fusarium head blight is one of the most important diseases affecting wheat yield and quality.It is of great significance to carry out intelligent monitoring of wheat Fusarium head blight for high yield,high quality and sustainable development of wheat.On the basis of identifying the harms of wheat Fusarium head blight,this paper analyzed the monitoring technology of wheat Fusarium head blight based on satellite remote sensing,hyperspectral,near-infrared,Internet of things and photoelectric system,to provide a reference for the intelligent monitoring of wheat Fusarium head blight.
基金supported by the National Natural Science Foundation of China (3315203911, 31471488)the National Key Research and Development Program of China(2016YFD0100602)the Transgenic Spe cial Item, China (2016ZX08002003-002 and 2016ZX08009-003)
文摘The mutation of the gene encoding a stearoyl-acyl carrier protein fatty acid desaturase（ssi2） has been proved to enhance pathogen resistance in several plants, while it＇s potential to regulate biotic and abiotic stresses in wheat is still unclear. In this study, we cloned TaSSI2 gene in wheat and provided several evidences of its involvement in multiple biological functions. By using barley stripe mosaic virus（BSMV）-induced gene silencing（VIGS） in wheat, it was found that TaSSI2 negatively regulated both powdery mildew and Fusarium head blight（FHB） resistance, which was consistent with the phenotype observed in knock-out mutants of Kronos. The expression of TaSSI2 was down-regulated by in vitro treatments of methyl jasmonate（Me JA）, but positively regulated by salicylic acid（SA） and abscisic acid（ABA）, implying the cross-talk between different hormone signaling pathways involved in wheat to regulate biotic stresses is still to be elucidated. Furthermore, the up-regulated expression of PR4 and PR5 indicated that TaSSI2 probably regulated FHB resistance by depressing the SA signaling pathway in wheat. In addition, the over-expression of TaSSI2 increased the content of linolenic acid（18：3） and subsequently enhanced drought tolerance of transgenic Brachypodium. This phenomenon might be associated with its subcellular localization in the whole cytosol, partly overlapping with Golgi apparatus and the secreted vesicles. As a stearoyl-acyl carrier protein fatty acid desaturase, TaSSI2 was proposed to be involved in cell lipid metabolism and carried targets out of the cell from membrane or wax synthesis, resulting in enhanced drought tolerance in plant.
基金supported by National Key Research and Development Program (2016YFD0101802, 2016YFD0101004)National Basic Research Program of China (2004CB117205, 2012CB125902)+5 种基金National Key Technology R&D Program of China (2002AA224161)National Science and Technology Major Project (2009ZX08009-049B, 2012ZX08009003)Jiangsu Collaborative Innovation Initiative for Modern Crop Production,'111' project B08025Natural Science Foundation of Jiangsu Province (BK20131316)Innovation Team Program for Jiangsu Universities (2014)the long-term funding from the National Science Foundation of China (30025030,30430440,30721140555,31030054,30671295)
文摘Fusarium head blight(FHB) or scab caused by Fusarium graminearum is a major threat to wheat production in China as well as in the world. To combat this disease, multiple efforts have been carried out internationally. In this article, we review our long-time effort in identifying the resistance genes and dissecting the resistance mechanisms by both forward and reverse genetics approaches in the last two decades. We present recent progress in resistance QTL identification, candidate functional gene discovery, marker-assisted improvement of FHB resistant varieties, and findings in investigating association of signal molecules, such as Ca^(++),SA, JA, and ET, with FHB response, with the assistance from rapidly growing genomics platforms. The information will be helpful for designing novel and efficient approaches to curb FHB.
基金financially supported by the National Key Research and Development Program of China (2017YFD0101000)International Scientific and Technological Cooperation Project (2016YFE0108600)Agricultural Science and Technology Innovation Program of CAAS
文摘The objective of this paper is to review progress made in wheat breeding for Fusarium head blight(FHB) resistance in China, the United States of America(USA), and Canada. In China,numerous Chinese landraces possessing high levels of FHB resistance were grown before the 1950 s. Later, pyramiding multiple sources of FHB resistance from introduced germplasm such as Mentana and Funo and locally adapted cultivars played a key role in combining satisfactory FHB resistance and high yield potential in commercial cultivars.Sumai 3, a Chinese spring wheat cultivar, became a major source of FHB resistance in the USA and Canada, and contributed to the release of more than 20 modern cultivars used for wheat production, including the leading hard spring wheat cultivars Alsen, Glenn, Barlow and SY Ingmar from North Dakota, Faller and Prosper from Minnesota, and AAC Brandon from Canada. Brazilian wheat cultivar Frontana, T. dicoccoides and other local germplasm provided additional sources of resistance. The FHB resistant cultivars mostly relied on stepwise accumulation of favorable alleles of both genes for FHB resistance and high yield,with marker-assisted selection being a valuable complement to phenotypic selection. With the Chinese Spring reference genome decoded and resistance gene Fhb1 now cloned, new genomic tools such as genomic selection and gene editing will be available to breeders, thus opening new possibilities for development of FHB resistant cultivars.
基金supported by the National Key Research and Development Program of China (2016YFD0101802, 2017YFD010060)the National Natural Science Foundation of China (31771881, 31401468)the Agricultural Science and Technology Innovation Program
文摘The gene Fhb1 has been used in many countries to improve wheat Fusarium head blight(FHB) resistance. To make better use of this gene in the Yellow-Huai River Valleys Winter Wheat Zone(YHWZ), the most important wheat-producing region of China, it is desirable to elucidate its effects on FHB resistance and agronomic traits in different genetic backgrounds. Based on a diagnostic marker for Fhb1, six BC2 populations were developed by crossing dwarf-male-sterile(DMS)-Zhoumai 16 to three Fhb1 donors(Ningmai 9, Ningmai 13, and Jianyang 84) and backcrossing to Zhoumai 16 and Zhoumai16’s derivative cultivars(Lunxuan 136 and Lunxuan 13) using marker-assisted backcross breeding. The progenies were assessed for FHB resistance and major agronomic traits.The Fhb1 alleles were identified using the gene-specific molecular marker. The plants with the Fhb1-resistant genotype(Fhb1-R) in these populations showed significantly fewer infected spikelets than those with the Fhb1-susceptible genotype(Fhb1-S). When Lunxuan 136 was used as the recurrent parent, Fhb1-R plants showed significantly fewer infected spikelets per spike than Fhb1-R plants produced using Lunxuan 13 as the recurrent parent, indicating that the genetic backgrounds of Fhb1 influence the expression of FHB resistance. Fhb1-R plants from the DMS-Zhoumai 16/Ningmai 9//Zhoumai 16/3/Lunxuan 136 population showed the highest FHB resistance among the six populations and a significantly higher level of FHB resistance than the moderately susceptible control Huaimai 20. No significant phenotypic differences between Fhb1-R and Fhb1-S plants were observed for the eight agronomic traits investigated. These results suggest that it is feasible to improve FHB resistance of winter wheat withoutreducing yield potential by introgressing Fhb1 resistance allele into FHB-susceptible cultivars in the YHWZ.
文摘Fusarium head blight (FHB) is a worldwide disease for wheat or barley. The contamination of important agricultural products with the trichothecene mycotoxins such as deoxynivalenol (DON) or 4,15-diacetoxyscirpe-
文摘Fusarium head blight (FHB) is a worldwide destructive disease of wheat in the warm, semi-humid or humid regions, especially serious in China. The disease not only causes significant losses in yield and reduces grain quanlity, but also induces toxin to contaminated seeds, which is harmful to the healthy of human and livestocks, So it is important to control it. There are several methods to control Fusarium head blight (FHB). Such as using Crop rotation, Soil cultivation and Fertiliser, biological control, Fungicides control, transgenes, resistance to control Fusarium head blight (FHB). All of these methods gain some effect, but also exist their deficiency. Sometimes crop rotation had no significant effect on DON contamination of wheat grain, subsequent reductions in DON contamination were inconsistent when using Soil cultivation and Fertiliser, Unfortunately, under field conditions, the biological control achieved has been shown to be variable and in some tests has failed to give any control, The use of fungicides, however, have not been consistently effective in controlling FHB and in reducing DON formation, transgene-silencing at different generations is a problem to use transgenes, Information on location of QTL for FHB resistance should improve dramatically in the near future on resistance to control Fusarium head blight (FHB). Therefore, it is pressing to improve control methods, especially to DON.
文摘Fusarium head blight, one of the most destructive diseases of wheat (Triticum aestivum L.), results in significant economic losses from reduced grain yield and quality. In recent decades, the disease has been frequently recorded, especially under warm and wet climatic conditions. Genetic resistance has engaged plant breeders because the use of resistant cultivars is the most economical, effective, and environmentally friendly method of control. In the present paper, we summarize the research on resistance genetics of Fusarium head blight, suggest a new method for evaluating Fusarium head blight resistance, and recommend strategies for creating and developing new sources of resistance to Fusarium head blight through the use of alien genes and chromosomal segments.
基金Bai for their strong support for this work. This study was supported by the Natural Science Foundation of Henan Province (NO. 222301420113, 232102520006)Major Science and Technology Special Project of Henan Province (NO. 221100210600)+2 种基金Henan Province key research and development project (NO. 231111110100)Key Scientific and Technological Project of Henan Province (NO. 242102111193)the Natural Science Foundation of China(NO. 31501225, 42101362).
文摘Fusarium head blight (FHB) is one of the most destructive diseases in global wheat production. In order to count the FHB-infected wheat ears under field conditions, this study proposed an algorithm for diseased wheat ear detection based on improved YOLOv5s (Tr-YOLOv5s). The Swin Transformer was used to replace the CSPDarknet backbone network to enhance the extraction of characteristic information of the population wheat ears of FHB in the field background. The convolutional block attention module (CBAM) attention mechanism was added to improve the detection effect of target wheat ears, subsequently improving the overall accuracy of the model. The original loss function complete intersection over union (CIoU) was replaced by Scylla intersection over union (SIoU) loss to accelerate the model convergence and decrease the loss value. The results showed that the mean average precision (mAP) of the Tr-YOLOv5s model reached 90.64%, making a 4.63% improvement compared to the original YOLOv5s model. The improved model could quickly detect and count wheat FHB ear in the field environment, which laid a foundation for the subsequent automatic disease identification and grading of wheat FHB under field conditions.
基金supported by the grant of the National High Technology Research and Development Program("863"Program)of China(No.2011AA100101)
文摘Thinopyrum elongatum （2n = 2x = 14, EE）, a wild relative of wheat, has been suggested as a potentially novel source of resistance to several major wheat diseases including Fusarium Head Blight （FHB）. In this study, a series of wheat （cv. Chinese Spring, CS） substitution and ditelosomic lines, including Th. elongatum additions, were assessed for Type II resistance to FHB. Results indicated that the lines containing chromosome 7E of Th. elongatum gave a high level of resistance to FHB, wherein the infection did not spread beyond the inoculated floret. Furthermore, it was determined that the novel resistance gene（s） of 7E was located on the short-ann （7ES） based on sharp difference in FHB resistance between the two 7E ditelosomic lines for each arm. On the other hand, Th. elongatum chromosomes 5E and 6E likely contain gene（s） for susceptibility to FHB because the disease spreads rapidly within the inoculated spikes of these lines. Genomic in situ hybridization （GISH） analysis revealed that the alien chromosomes in the addition and substitution lines were intact, and the lines did not contain discernible genomic aberrations. GISH and multicolor-GISH analyses were further performed on three trans- location lines that also showed high levels of resistance to FHB. Lines TA3499 and TA3695 were shown to contain one pair of wheat-Th. elongatum translocated chromosomes involving fragments of 7D plus a segment of the 7E, while line TA3493 was found to contain one pair of wheat-Th, elongatum translocated chromosomes involving the D- and A-genome chromosomes of wheat. Thus, this study has established that the short-arm of chromosome 7E of Th. elongatum harbors gene（s） highly resistant to the spreading of FHB, and chromatin of 7E introgressed into wheat chromosomes largely retained the resistance, implicating the feasibility of using these lines as novel material for breeding FHB-resistant wheat cultivars.
基金supported partially by the National Key Project for the Research and Development of China(2017YFE0126700)Jiangsu Agricultural Science and Technology Innovation Fund(CX(21)3109)Jiangsu seed Industry Revitalization Project(JBGS(2021)052).
文摘Thaumatin-like proteins (TLPs) play potential roles in plant resistance to various diseases. Identifying TLPs is neces-sary to determine their function and apply them to plant disease resistance. However, limited information is available about TLP-family genes in wheat, especially regarding their responses to Fusarium species, which cause Fusarium head blight in wheat. In this study, we conducted a comprehensive genome-wide survey of TLP genes in wheat and identified 129 TLP genes in the wheat genome, which were unevenly distributed on 21 wheat chromosomes, with 5A containing the highest number. Phylogenetic analysis showed that these 129 wheat TLP genes together with 24 Arabidopsis TLPs were classified into 7 groups based on the protein sequences. We systematically analyzed the genes in terms of their sequence characterization, chromosomal locations, exon-intron distribution, duplication (tandem and segmental) events and expression profiles in response to Fusarium infection. Furthermore, we analyzed differen-tially expressed TLP genes based on publicly available RNA-seq data obtained from a resistant near isogenic wheat line at different time points after Fusarium graminearum inoculation. Then, the expression of 9 differentially expressed TLP genes was confirmed by real-time PCR, and these 9 genes were all upregulated in the resistant Sumai 3 variety, which was generally consistent with the RNA-seq data. Our results provide a basis for selecting candidate wheat TLP genes for further studies to determine the biological functions of the TLP genes in wheat.
