The structures of human telomeric DNA have received much attention due to its significant biological importance.Most studies have focused on G-quadruplex structure formed by short telomeric DNA sequence,but little is ...The structures of human telomeric DNA have received much attention due to its significant biological importance.Most studies have focused on G-quadruplex structure formed by short telomeric DNA sequence,but little is known about the structures of long singlestranded telomeric DNAs.Here,we investigated the structure of DNA with a long sequence of d[AGGG(TTAGGG)6](G6-DNA)and the effect of a single repeat sequence d(TTAGGG)(G01-DNA)on the structure of G6-DNA using sedimentation velocity technique,polyacrylamide gel electrophoresis,circular dichroism spectroscopy,and UV melting experiments.The results suggest that the G6-DNA can form dimers in aqueous solutions and G01-DNA can form additional G-quadruplex structures by binding to G6-DNA.However,G01-DNA has no effect on the structure of DNA with a sequence of d[AGGG(TTAGGG)3](G3-DNA).Our study provides new insights into the structure polymorphism of long human single-stranded telomeric DNA.展开更多
基金supported by the National Natural Scientific Foundation of China (No.21674107)the Fundamental Research Funds for the Central Universities (No.WK2340000066)
文摘The structures of human telomeric DNA have received much attention due to its significant biological importance.Most studies have focused on G-quadruplex structure formed by short telomeric DNA sequence,but little is known about the structures of long singlestranded telomeric DNAs.Here,we investigated the structure of DNA with a long sequence of d[AGGG(TTAGGG)6](G6-DNA)and the effect of a single repeat sequence d(TTAGGG)(G01-DNA)on the structure of G6-DNA using sedimentation velocity technique,polyacrylamide gel electrophoresis,circular dichroism spectroscopy,and UV melting experiments.The results suggest that the G6-DNA can form dimers in aqueous solutions and G01-DNA can form additional G-quadruplex structures by binding to G6-DNA.However,G01-DNA has no effect on the structure of DNA with a sequence of d[AGGG(TTAGGG)3](G3-DNA).Our study provides new insights into the structure polymorphism of long human single-stranded telomeric DNA.
