Gibberellin 2-oxidases(GA2ox)are important enzymes that maintain the balance of bioactive GAs in plants.GA2ox genes have been identified and characterized in many plants,but these genes were not investigated in Brassi...Gibberellin 2-oxidases(GA2ox)are important enzymes that maintain the balance of bioactive GAs in plants.GA2ox genes have been identified and characterized in many plants,but these genes were not investigated in Brassica napus.Here,we identified 31 GA2ox genes in B.napus and 15 of these BnaGA2ox genes were distributed in the A and C subgenomes.Subcellular localization predictions suggested that all BnaGA2ox proteins were localized in the cytoplasm,and gene structure analysis showed that the BnaGA2ox genes contained 2–4 exons.Phylogenetic analysis indicated that BnGA2ox family proteins in monocotyledons and dicotyledons can be divided into four groups,including two C_(19)-GA2ox and two C_(20)-GA2ox clades.Group 4 is a C_(20)-GA2ox Class discovered recently.Most BnaGA2ox genes had a syntenic relationship with AtGA2ox genes.BnaGA2ox genes in the C subgenome had experienced stronger selection pressure than genes in the A subgenome.BnaGA2ox genes were highly expressed in specific tissues such as those involved in growth and development,and most of them were mainly involved in abiotic responses,regulation of phytohormones and growth and development.Our study provided a valuable evolutionary analysis of GA2ox genes in monocotyledons and dicotyledons,as well as an insight into the biological functions of GA2ox family genes in B.napus.展开更多
以直接水热合成和低硅沸石高温水蒸气脱铝两种方法制备了不同Si/Al摩尔比的HZSM-5载体,考察了Ga2O3/HZSM-5催化剂上丙烷脱氢制丙烯的反应性能.结合XRD,N2气吸附,27Al MAS NMR和NH3-TPD等表征方法,讨论了催化剂酸性质和孔性质与性能的关...以直接水热合成和低硅沸石高温水蒸气脱铝两种方法制备了不同Si/Al摩尔比的HZSM-5载体,考察了Ga2O3/HZSM-5催化剂上丙烷脱氢制丙烯的反应性能.结合XRD,N2气吸附,27Al MAS NMR和NH3-TPD等表征方法,讨论了催化剂酸性质和孔性质与性能的关系.实验结果表明,减少催化剂表面的中强酸量可以显著提高反应的稳定性和丙烯的选择性;水蒸气脱铝所增加的介孔有同样的效果,这可能是由于介孔的存在有利于丙烯的脱附和扩散而抑制了寡聚、环化等副反应的发生,减少了表面积炭的可能性.展开更多
基金supported by the Chongqing Academy of Agricultural Sciences Youth Innovation Team Project(NKY-2018QC01)Chongqing Finance Special Project(NKY-2022AC002)+2 种基金the Natural Science Foundation Project of Yongchuan(2021yc-jckx20013)the Technology Innovation and Application Development(Surface)Project of Yongchuan(2021yc-cxfz30007)the National Oilseed Rape Industrial Technology System Sanxia Comprehensive Experiment Station Project(CARS-13).
文摘Gibberellin 2-oxidases(GA2ox)are important enzymes that maintain the balance of bioactive GAs in plants.GA2ox genes have been identified and characterized in many plants,but these genes were not investigated in Brassica napus.Here,we identified 31 GA2ox genes in B.napus and 15 of these BnaGA2ox genes were distributed in the A and C subgenomes.Subcellular localization predictions suggested that all BnaGA2ox proteins were localized in the cytoplasm,and gene structure analysis showed that the BnaGA2ox genes contained 2–4 exons.Phylogenetic analysis indicated that BnGA2ox family proteins in monocotyledons and dicotyledons can be divided into four groups,including two C_(19)-GA2ox and two C_(20)-GA2ox clades.Group 4 is a C_(20)-GA2ox Class discovered recently.Most BnaGA2ox genes had a syntenic relationship with AtGA2ox genes.BnaGA2ox genes in the C subgenome had experienced stronger selection pressure than genes in the A subgenome.BnaGA2ox genes were highly expressed in specific tissues such as those involved in growth and development,and most of them were mainly involved in abiotic responses,regulation of phytohormones and growth and development.Our study provided a valuable evolutionary analysis of GA2ox genes in monocotyledons and dicotyledons,as well as an insight into the biological functions of GA2ox family genes in B.napus.
文摘以直接水热合成和低硅沸石高温水蒸气脱铝两种方法制备了不同Si/Al摩尔比的HZSM-5载体,考察了Ga2O3/HZSM-5催化剂上丙烷脱氢制丙烯的反应性能.结合XRD,N2气吸附,27Al MAS NMR和NH3-TPD等表征方法,讨论了催化剂酸性质和孔性质与性能的关系.实验结果表明,减少催化剂表面的中强酸量可以显著提高反应的稳定性和丙烯的选择性;水蒸气脱铝所增加的介孔有同样的效果,这可能是由于介孔的存在有利于丙烯的脱附和扩散而抑制了寡聚、环化等副反应的发生,减少了表面积炭的可能性.
