The cis-regulatory elements of promoters regulate temporal and spatial expression of genes. DNA inethylation, histone methylation and histone acetylation are the main types of epigenetic modifications, which play impo...The cis-regulatory elements of promoters regulate temporal and spatial expression of genes. DNA inethylation, histone methylation and histone acetylation are the main types of epigenetic modifications, which play important roles in plant growth and development. DNA methylation could seilenco transposons, affect gene imprinting and gene expression. In this study, we found that granule bound starch synthase 1 （GBSSI） gene is expressed specifically in wheat endosperm rath- er than in the embryo. We also analyzed the cis-elements within this promoter region and found some seed-specific elements. In order to confirm the tissue specifici- ty, we cloned 4k bp sequences upstream of GBSS1 gene to link to vector with GUS and this construct was transferred to tobacco by Agrobacterium mediated transfor- marion. The results showed that wheat GBSS1 promoter mediated the seed-specific expression of GUS gene, hut not mediated expression in embryo. In addition, we found that GBSSI promoter is methylated in wheat embryo and de-methylated in wheat endosperm. Our study might provide the molecular basis for specific expres- sion of GBSSI gene.展开更多
目的探讨DBP(D site of albumin promoter(albumin D-box)binding protein)及E4BP4(腺病毒E4启动子结合蛋白-4)是否参与拓扑异构酶Ⅰ(TOPOⅠ)的调节作用。方法运用荧光素酶转录活性测定DBP和E4BP4对mTopoⅠ的转录活性,凝胶阻滞分析检测...目的探讨DBP(D site of albumin promoter(albumin D-box)binding protein)及E4BP4(腺病毒E4启动子结合蛋白-4)是否参与拓扑异构酶Ⅰ(TOPOⅠ)的调节作用。方法运用荧光素酶转录活性测定DBP和E4BP4对mTopoⅠ的转录活性,凝胶阻滞分析检测DBP及E4BP4蛋白和TopoⅠ核酸相互作用。结果荧光素酶转录活性测定实验显示DBP及E4BP4均能促进TopoⅠ基因的转录活性;凝胶阻滞分析实验直接证实DBP及E4BP4与TOPOⅠ启动子区域D-box活性区域结合发挥活性作用。结论 DBP及E4BP4参与调节拓扑异构酶Ⅰ的表达。展开更多
基金Supported by Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences(CXGC2016D01)
文摘The cis-regulatory elements of promoters regulate temporal and spatial expression of genes. DNA inethylation, histone methylation and histone acetylation are the main types of epigenetic modifications, which play important roles in plant growth and development. DNA methylation could seilenco transposons, affect gene imprinting and gene expression. In this study, we found that granule bound starch synthase 1 （GBSSI） gene is expressed specifically in wheat endosperm rath- er than in the embryo. We also analyzed the cis-elements within this promoter region and found some seed-specific elements. In order to confirm the tissue specifici- ty, we cloned 4k bp sequences upstream of GBSS1 gene to link to vector with GUS and this construct was transferred to tobacco by Agrobacterium mediated transfor- marion. The results showed that wheat GBSS1 promoter mediated the seed-specific expression of GUS gene, hut not mediated expression in embryo. In addition, we found that GBSSI promoter is methylated in wheat embryo and de-methylated in wheat endosperm. Our study might provide the molecular basis for specific expres- sion of GBSSI gene.
文摘目的探讨DBP(D site of albumin promoter(albumin D-box)binding protein)及E4BP4(腺病毒E4启动子结合蛋白-4)是否参与拓扑异构酶Ⅰ(TOPOⅠ)的调节作用。方法运用荧光素酶转录活性测定DBP和E4BP4对mTopoⅠ的转录活性,凝胶阻滞分析检测DBP及E4BP4蛋白和TopoⅠ核酸相互作用。结果荧光素酶转录活性测定实验显示DBP及E4BP4均能促进TopoⅠ基因的转录活性;凝胶阻滞分析实验直接证实DBP及E4BP4与TOPOⅠ启动子区域D-box活性区域结合发挥活性作用。结论 DBP及E4BP4参与调节拓扑异构酶Ⅰ的表达。
