目的：探讨微波辐射对小鼠GC．2spd细胞的影响。方法：培养的GC-2spd细胞经平均功率密度为0、10、30mW／cm。微波辐射15min，于辐射后1-24h，应用MTF法、光镜、电镜、流式细胞术和ELISA法观测细胞增殖活力、细胞形态学和超微结构、细胞...目的：探讨微波辐射对小鼠GC．2spd细胞的影响。方法：培养的GC-2spd细胞经平均功率密度为0、10、30mW／cm。微波辐射15min，于辐射后1-24h，应用MTF法、光镜、电镜、流式细胞术和ELISA法观测细胞增殖活力、细胞形态学和超微结构、细胞凋亡及cAMP含量的变化。结果：10、30mW／cm2微波辐射后1-24h（除30mW／cm2辐射后12h外），GC-2spd细胞增殖活力较对照组明显下降（P〈0．01或P〈0．05），光镜见部分细胞突起变短，数目减少，胞内空泡增多；辐射后6h，细胞凋亡率（％）明显增加（14．59±1．09、8．48±1．73傩4．56±2．09，P〈0．05或P〈0．01），电镜见细胞核染色质凝集边移或核膜破裂，内质网明显扩张；辐射后6h和24h，细胞内cAMP含量（nmol／g）明显降低（1．65±0．17、1．96±0．10 vs 2．77±0．24，2．13±0．33、1．69±0．27 vs 3．02±0．47，P〈0．05或P〈0．01）。结论：10和30mW／cm2微波辐射可引起GC-2spd细胞损伤，表现为细胞内cAMP含量降低，细胞增殖活力下降，细胞凋亡增加。展开更多
RING finger 187(RNF187),a ubiquitin-ligating(E3)enzyme,plays a crucial role in the proliferation of cancer cells.However,it remains unclear whether RNF187 exhibits comparable functionality in the development of germli...RING finger 187(RNF187),a ubiquitin-ligating(E3)enzyme,plays a crucial role in the proliferation of cancer cells.However,it remains unclear whether RNF187 exhibits comparable functionality in the development of germline cells.To investigate thepotential involvement of RNF187 in germ cell development,we conducted interference and overexpression assays using GC-2 cells,a mouse spermatocyte-derived cell line.Our findings reveal that the interaction between RNF187 and histone H3 increases theviability,proliferation,and migratory capacity of GC-2 cells.Moreover,we provide evidence demonstrating that RNF187 interactswith H3 and mediates the ubiquitination of H3 at lysine 57(K57)or lysine 80(K80),directly or indirectly resulting in increasedcellular transcription.This is a study to report the role of RNF187 in maintaining the development of GC-2 cells by mediatinghistone H3 ubiquitination,thus highlighting the involvement of the K57 and K80 residues of H3 in the epistatic regulation of genetranscription.These discoveries provide a new theoretical foundation for further comprehensive investigations into the functionof RNF187 in the reproductive system.展开更多
目的探讨邻苯二甲酸二(2-乙基己)酯(di-2-ethylhexyl phthalate,DEHP)对GC-2spd细胞Bcl-2、Bax m RNA表达的影响。方法体外培养GC-2spd细胞,用不同浓度的DEHP(0、50、100和200μmol/L)染毒24 h。采用MTT实验检测细胞存活率,Annexin V-FI...目的探讨邻苯二甲酸二(2-乙基己)酯(di-2-ethylhexyl phthalate,DEHP)对GC-2spd细胞Bcl-2、Bax m RNA表达的影响。方法体外培养GC-2spd细胞,用不同浓度的DEHP(0、50、100和200μmol/L)染毒24 h。采用MTT实验检测细胞存活率,Annexin V-FITC/PI双染色法结合流式细胞仪检测细胞凋亡率,荧光定量PCR法检测Bcl-2和Bax的m RNA表达。结果在DEHP染毒细胞24 h后,随着DEHP浓度(0、50、100和200μmol/L)的增加,细胞存活率逐渐下降,细胞凋亡率逐渐上升,Bcl-2 m RNA表达水平下降,Bax m RNA表达水平上升。结论 DEHP可能通过线粒体通路诱导GC-2spd细胞凋亡,进而影响雄性生殖功能。展开更多
文摘目的：探讨微波辐射对小鼠GC．2spd细胞的影响。方法：培养的GC-2spd细胞经平均功率密度为0、10、30mW／cm。微波辐射15min，于辐射后1-24h，应用MTF法、光镜、电镜、流式细胞术和ELISA法观测细胞增殖活力、细胞形态学和超微结构、细胞凋亡及cAMP含量的变化。结果：10、30mW／cm2微波辐射后1-24h（除30mW／cm2辐射后12h外），GC-2spd细胞增殖活力较对照组明显下降（P〈0．01或P〈0．05），光镜见部分细胞突起变短，数目减少，胞内空泡增多；辐射后6h，细胞凋亡率（％）明显增加（14．59±1．09、8．48±1．73傩4．56±2．09，P〈0．05或P〈0．01），电镜见细胞核染色质凝集边移或核膜破裂，内质网明显扩张；辐射后6h和24h，细胞内cAMP含量（nmol／g）明显降低（1．65±0．17、1．96±0．10 vs 2．77±0．24，2．13±0．33、1．69±0．27 vs 3．02±0．47，P〈0．05或P〈0．01）。结论：10和30mW／cm2微波辐射可引起GC-2spd细胞损伤，表现为细胞内cAMP含量降低，细胞增殖活力下降，细胞凋亡增加。
基金supported by the National Natural Science Foundation ofChina(82271633 to BZ and 82201762 to TTG)the Gusu Health Talent Programof Suzhou(GSWS2020068 to BZ)+2 种基金the Top Talent Support Program for Youngand Middle-aged People of Wuxi Health Committee(BJ2020047 to YBW)theScience and Technology Project of Changzhou(CJ20220143 to TTG)the Changzhou Health Committee Funded Young Investigator Training Project(CZQM2020099 to TTG)。
文摘RING finger 187(RNF187),a ubiquitin-ligating(E3)enzyme,plays a crucial role in the proliferation of cancer cells.However,it remains unclear whether RNF187 exhibits comparable functionality in the development of germline cells.To investigate thepotential involvement of RNF187 in germ cell development,we conducted interference and overexpression assays using GC-2 cells,a mouse spermatocyte-derived cell line.Our findings reveal that the interaction between RNF187 and histone H3 increases theviability,proliferation,and migratory capacity of GC-2 cells.Moreover,we provide evidence demonstrating that RNF187 interactswith H3 and mediates the ubiquitination of H3 at lysine 57(K57)or lysine 80(K80),directly or indirectly resulting in increasedcellular transcription.This is a study to report the role of RNF187 in maintaining the development of GC-2 cells by mediatinghistone H3 ubiquitination,thus highlighting the involvement of the K57 and K80 residues of H3 in the epistatic regulation of genetranscription.These discoveries provide a new theoretical foundation for further comprehensive investigations into the functionof RNF187 in the reproductive system.
文摘目的探讨邻苯二甲酸二(2-乙基己)酯(di-2-ethylhexyl phthalate,DEHP)对GC-2spd细胞Bcl-2、Bax m RNA表达的影响。方法体外培养GC-2spd细胞,用不同浓度的DEHP(0、50、100和200μmol/L)染毒24 h。采用MTT实验检测细胞存活率,Annexin V-FITC/PI双染色法结合流式细胞仪检测细胞凋亡率,荧光定量PCR法检测Bcl-2和Bax的m RNA表达。结果在DEHP染毒细胞24 h后,随着DEHP浓度(0、50、100和200μmol/L)的增加,细胞存活率逐渐下降,细胞凋亡率逐渐上升,Bcl-2 m RNA表达水平下降,Bax m RNA表达水平上升。结论 DEHP可能通过线粒体通路诱导GC-2spd细胞凋亡,进而影响雄性生殖功能。
