Ionizing radiation is a popular and effective treatment option for glioblastoma(GBM).However,resistance to radiation therapy inevitably occurs during treatment.It is urgent to investigate the mechanisms of radioresist...Ionizing radiation is a popular and effective treatment option for glioblastoma(GBM).However,resistance to radiation therapy inevitably occurs during treatment.It is urgent to investigate the mechanisms of radioresistance in GBM and to find ways to improve radiosensitivity.Here,we found that heat shock protein 90 beta family member 1(HSP90B1)was significantly upregulated in radioresistant GBM cell lines.More importantly,HSP90B1 promoted the localization of glucose transporter type 1,a key rate-limiting factor of glycolysis,on the plasma membrane,which in turn enhanced glycolytic activity and subsequently tumor growth and radioresistance of GBM cells.These findings imply that targeting HSP90B1 may effectively improve the efficacy of radiotherapy for GBM patients,a potential new approach to the treatment of glioblastoma.展开更多
目的探讨人肺癌组织中GLUT1的表达量与其葡萄糖摄取量之间的相关性.方法术前采用符合线路18F-2-脱氧葡萄糖-单光子发射计算机体层扫描(FDG-SPECT with coincidence)技术检测30例肺癌患者肿瘤细胞对葡萄糖的类似物-18F-2-脱氧葡萄糖(FDG...目的探讨人肺癌组织中GLUT1的表达量与其葡萄糖摄取量之间的相关性.方法术前采用符合线路18F-2-脱氧葡萄糖-单光子发射计算机体层扫描(FDG-SPECT with coincidence)技术检测30例肺癌患者肿瘤细胞对葡萄糖的类似物-18F-2-脱氧葡萄糖(FDG)摄取,FDG-SPECT扫描结果是以对目测结果进行分析得出的,以肺部病变/正常肺组织的FGD摄取比值(T/N)作为肿瘤对葡萄糖的摄取量;用免疫组化sp法检测相应肺癌患者癌组织中GLUT1的表达,实验结果以图像分析与处理系统进行半定量处理.结果30例肺癌组织中GLUT1表达均为阳性,与术后病理结果相对照,FDG-SPECT扫描结果有一例假阴性.用直线相关统计分析,发现FDG摄取值与GLUT1表达量具有很高的相关性(r=0.87,P＜0.01).结论GLUT1在肺癌组织的葡萄糖代谢中起到重要的作用,其过度表达可能是肺癌患者葡萄糖摄取的分子基础.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.82072765 to X.Q.and 82172667 to X.W.).
文摘Ionizing radiation is a popular and effective treatment option for glioblastoma(GBM).However,resistance to radiation therapy inevitably occurs during treatment.It is urgent to investigate the mechanisms of radioresistance in GBM and to find ways to improve radiosensitivity.Here,we found that heat shock protein 90 beta family member 1(HSP90B1)was significantly upregulated in radioresistant GBM cell lines.More importantly,HSP90B1 promoted the localization of glucose transporter type 1,a key rate-limiting factor of glycolysis,on the plasma membrane,which in turn enhanced glycolytic activity and subsequently tumor growth and radioresistance of GBM cells.These findings imply that targeting HSP90B1 may effectively improve the efficacy of radiotherapy for GBM patients,a potential new approach to the treatment of glioblastoma.
文摘目的探讨人肺癌组织中GLUT1的表达量与其葡萄糖摄取量之间的相关性.方法术前采用符合线路18F-2-脱氧葡萄糖-单光子发射计算机体层扫描(FDG-SPECT with coincidence)技术检测30例肺癌患者肿瘤细胞对葡萄糖的类似物-18F-2-脱氧葡萄糖(FDG)摄取,FDG-SPECT扫描结果是以对目测结果进行分析得出的,以肺部病变/正常肺组织的FGD摄取比值(T/N)作为肿瘤对葡萄糖的摄取量;用免疫组化sp法检测相应肺癌患者癌组织中GLUT1的表达,实验结果以图像分析与处理系统进行半定量处理.结果30例肺癌组织中GLUT1表达均为阳性,与术后病理结果相对照,FDG-SPECT扫描结果有一例假阴性.用直线相关统计分析,发现FDG摄取值与GLUT1表达量具有很高的相关性(r=0.87,P＜0.01).结论GLUT1在肺癌组织的葡萄糖代谢中起到重要的作用,其过度表达可能是肺癌患者葡萄糖摄取的分子基础.
