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Molecular Characterization of a Triticum durum-Haynaldia villosa Amphiploid and Its Derivatives for Resistance to Gaeumannomyces graminis var. tritici
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作者 HUANGDa-hui LIN Zhi-shan +4 位作者 CHEN Xiao ZHANG Zeng-yan CHEN Cai-ceng CHENG Shun-he XIN Zhi-yong 《Agricultural Sciences in China》 CAS CSCD 2007年第5期513-521,共9页
Take-all is a serious disease found in wheat across the world. Haynaldia villosa is considered to be resistant to take-all at a high level. TH3 was an amphiploid (2n =42, AABBVV) between Triticum durum and Haynaldia... Take-all is a serious disease found in wheat across the world. Haynaldia villosa is considered to be resistant to take-all at a high level. TH3 was an amphiploid (2n =42, AABBVV) between Triticum durum and Haynaldia viUosa with significant resistance to take-all fungus isolated from China. In greenhouse experiment, the derivatives of the hybrid between wheat and TH3 showed better resistance to take-all than that of the wheat control. One of the derivatives named HW918-5 was selected for further analysis. Cytological and genomic in situ hybridization (GISH) analysis indicated that a monotelosome originated from H. villosa existed in the genome of the offspring of the line HW918-5. The monotelosome with promising resistant gene for take-all was located on the 3V chromosome of H. villosa in the further PCR-based molecular analysis. 展开更多
关键词 AFLP gaeumannomyces graminis var. tritici genomic in situ hybridization Haynaldia villosa Triticum aestivum
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