Investigating gastrointestinal(GI)motility disorders relies on diagnostic tools to assess muscular contractions,peristalsis propagation and the integrity and coordination of various sphincters.Manometries are the gold...Investigating gastrointestinal(GI)motility disorders relies on diagnostic tools to assess muscular contractions,peristalsis propagation and the integrity and coordination of various sphincters.Manometries are the gold standard to study the GI motor function but it is increasingly acknowledged that manometries do not provide a complete picture in relation to sphincters competencies and muscle fibrosis.Endolumenal functional lumen imaging probe(EndoFLIP)an emerging technology,uses impedance planimetry to measure hollow organs cross sectional area,distensibility and compliance.It has been successfully used as a complementary tool in the assessment of the upper and lower oesophageal sphincters,oesophageal body,the pylorus and the anal canal.In this article,we aim to review the uses of EndoFLIP as a tool to investigate GI motility disorders with a special focus on paediatric practice.The majority of EndoFLIP studies were conducted in adult patients but the uptake of the technology in paediatrics is increasing.EndoFLIP can provide a useful complementary data to the existing GI motility investigation in both children and adults.展开更多
Objective:To investigate the effect of auricular acupuncture on postoperative pain and gastrointestinal motility recovery after laparoscopic surgery for colorectal cancer.Methods:The clinical data of patients who unde...Objective:To investigate the effect of auricular acupuncture on postoperative pain and gastrointestinal motility recovery after laparoscopic surgery for colorectal cancer.Methods:The clinical data of patients who underwent laparoscopic radical surgery for colorectal cancer in our hospital from April 2020 to December 2021 were collected.Based on the inclusion and exclusion criteria,76 patients were included in the retrospective analysis.Depending on whether they received auricular acupuncture or not,the patients were divided into two groups:46 patients in the experimental group(auricular acupuncture)and 30 patients in the control group.The differences between the two groups were analyzed.Results:The time to first flatus of the experimental group was significantly shorter than that of the control group(52.2±7.36 h versus 66.3±7.83 h;P<0.001).Similarly,the time to first defecation of the experimental group was significantly shorter than that of the control group(76.3±7.76 h versus 86.1±10.79 h;P<0.001).The time to first fluid intake of the auricular group was significantly shorter than that of the control group(90.4±8.92 h versus 107.3±9.66 h,P<0.001).Compared with the control group,the experimental group scored significantly lower on the visual analogue scale on postoperative days 2 and 3(P<0.001).Conclusion:Auricular acupuncture is an effective traditional Chinese medicine external treatment method.It can promote gastrointestinal motility recovery in patients after laparoscopic radical surgery for colorectal cancer and also reduce postoperative pain and discomfort.Furthermore,this therapy is easy to operate and well-accepted by patients.Therefore,it should be strongly promoted in clinical practice.展开更多
Acupuncture has been used in China for thousands of years and has become more widely accepted by doctors and patients around the world. A large number of clinical studies and animal experiments have confirmed that acu...Acupuncture has been used in China for thousands of years and has become more widely accepted by doctors and patients around the world. A large number of clinical studies and animal experiments have confirmed that acupuncture has a benign adjustment effect on gastrointestinal(GI) movement;however, the mechanism of this effect is unclear, especially in terms of neural mechanisms, and there are still many areas that require further exploration. This article reviews the recent data on the neural mechanism of acupuncture on GI movements. We summarize the neural mechanism of acupuncture on GI movement from four aspects: acupuncture signal transmission, the sympathetic and parasympathetic nervous system, the enteric nervous system, and the central nervous system.展开更多
AIM:To investigate the therapeutic effect of Schistosoma mansoni(S.mansoni) soluble worm proteins on gastrointestinal motility disturbances during experimental colitis in mice. METHODS:Colitis was induced by intrarect...AIM:To investigate the therapeutic effect of Schistosoma mansoni(S.mansoni) soluble worm proteins on gastrointestinal motility disturbances during experimental colitis in mice. METHODS:Colitis was induced by intrarectal injection of trinitrobenzene sulphate(TNBS) and 6 h later,mice were treated ip with S.mansoni proteins.Experiments were performed 5 d after TNBS injection.Inflammationwas quantified using validated inflammation parameters. Gastric emptying and geometric center were measured to assess in vivo gastrointestinal motility.Peristaltic activity of distal colonic segments was studied in vitro using a modified Trendelenburg set-up.Cytokine profiles of T-lymphocytes isolated from the colon were determined by real time reverse transcriptase-polymerase chain reaction. RESULTS:Intracolonic injection of TNBS caused severe colitis.Treatment with S.mansoni proteins significantly ameliorated colonic inflammation after 5 d.TNBS did not affect gastric emptying but significantly decreased the geometric center and impaired colonic peristaltic activity 5 d after the induction of colitis.Treatment with S.mansoni proteins ameliorated these in vivo and in vitro motility disturbances.In addition,TNBS injection caused a downregulation of effector T cell cytokines after 5 d,whereas a S.mansoni protein effect was no longer observed at this time point. CONCLUSION:Treatment with S.mansoni proteins attenuated intestinal inflammation and ameliorated motility disturbances during murine experimental colitis.展开更多
AIM: To correlate gastric contractility, gastrointestinal transit, and hormone levels in non-pregnant(estrous cycle) and pregnant rats using noninvasive techniques. METHODS: Female rats(n = 23) were randomly divided i...AIM: To correlate gastric contractility, gastrointestinal transit, and hormone levels in non-pregnant(estrous cycle) and pregnant rats using noninvasive techniques. METHODS: Female rats(n = 23) were randomly divided into(1) non-pregnant,(contractility, n =6; transit, n = 6); and(2) pregnant(contractility, n = 5; transit, n = 6). In each estrous cycle phase or at 0, 7, 14, and 20 d after the confirmation of pregnancy, gastrointestinal transit was recorded by AC biosusceptometry(ACB), and gastric contractility was recorded by ACB and electromyography. After each recording, blood samples were obtained for progesterone and estradiol determination. RESULTS: In the estrous cycle, despite fluctuations of sex hormone levels, no significant changes in gastrointestinal motility were observed. Days 7 and 14 of pregnancy were characterized by significant changes in the frequency of contractions(3.90 ± 0.42 cpm and 3.60 ± 0.36 cpm vs 4.33 ± 0.25 cpm) and gastric emptying(168 ± 17 min and 165 ± 15 min vs 113 ± 15 min) compared with day 0. On these same days, progesterone levels significantly increased compared with control(54.23 ± 15.14 ng/m L and 129.96 ± 30.52 ng/mL vs 13.25 ± 6.31 ng/mL). On day 14, we observed the highest level of progesterone and the lowest level of estradiol compared with day 0(44.3 ± 15.18 pg/mL vs 24.96 ± 5.96 pg/mL). CONCLUSION: Gastrointestinal motility was unaffected by the estrous cycle. In our data, high progesterone and low estradiol levels can be associated with decreased contraction frequency and slow gastric emptying.展开更多
Functional dyspepsia(FD)is a regularly diagnosed clinical gastrointestinal ailment with a high incidence rate that can considerably impact patients’health and quality of life and impose a substantial financial burden...Functional dyspepsia(FD)is a regularly diagnosed clinical gastrointestinal ailment with a high incidence rate that can considerably impact patients’health and quality of life and impose a substantial financial burden.Modern research on the pathophysiology of functional dyspepsia has not thoroughly explained the underlying reasons.The condition does not manifest any significant organ abnormalities,which raises the disease’s difficulty coefficient.Major pathogenic exceptions in FD include gastrointestinal motor dysfunction,gastrointestinal hormone secretion problem,visceral hypersensitivity,and brain-gut axis.Several ion channels have reportedly been implicated in the pathophysiological process of FD.Therefore,it is crucial to comprehend the probable activities of various ion channels in FD.This study focuses on the current state of research on the possible role of several ion channels in the pathogenesis of FD.展开更多
Gastric dyskinesis functional dyspepsia is a commonly clinical condition characterized as epigastric discomfort,distension after meals,early satiety and belching,which are commonly associated with the dysfunction of g...Gastric dyskinesis functional dyspepsia is a commonly clinical condition characterized as epigastric discomfort,distension after meals,early satiety and belching,which are commonly associated with the dysfunction of gastrointestinal motility.展开更多
Background: Previous studies on fasting and gastrointestinal motility were reported with information lacking concerning prolonged continuous fasting and gastrointestinal motility. This study investigated the effect of...Background: Previous studies on fasting and gastrointestinal motility were reported with information lacking concerning prolonged continuous fasting and gastrointestinal motility. This study investigated the effect of prolonged fasting duration on gastrointestinal motility. Methods: Forty-five (45) male Wistar rats, with body weights between 180 - 200 g were used. They were randomly assigned into three (3) groups. Group1: control (rats fasted for 18 h—common duration of fasting for motility studies), groups 2 and 3 fasted for 48 and 72 h respectively. Five (5) rats per experiment and per group were considered. Blood glucose was determined by glucose oxidase method, gastric emptying was assessed by hydrated carbohydrate meal, intestinal motility by charcoal meal, and colonic motility was assessed using bead test. Data were reported in Mean ± SEM and analyzed with one-way ANOVA. Differences in results were considered significant at p ≤ 0.05. Results: There was no significant change in the blood glucose level (mmol/L) of rats in the 48 h group (2.94 ± 0.35) and 72 h group (3.20 ± 0.32) as compared with the control (3.62 ± 0.19). There was a significant decrease in the rate of gastric emptying (g) in the 72 h group (0.20 ± 0.08) compared with the control (0.64 ± 0.16). The intestinal transit (cm) in the 48 h group (67.54 ± 6.15) and 72 h group (72.10 ± 7.60) increased significantly when compared with the control (42.14 ± 3.14). There was a significant decrease in the colonic motility time (Sec.) in the 48 h group (2707 ± 864.1) and 72 h group (6363 ± 968.1) when compared with the control (263.8 ± 64.26). Conclusion: Extended fasting durations decrease the rate of gastric emptying and colonic motility. It suggests that extended fasting durations could be beneficial in intestinal spasms or where the gut is required to relax.展开更多
Interstitial cells of Cajal (ICC) are important players in the symphony of gut motility. They have a very signif icant physiological role orchestrating the normal peristaltic activity of the digestive system. They are...Interstitial cells of Cajal (ICC) are important players in the symphony of gut motility. They have a very signif icant physiological role orchestrating the normal peristaltic activity of the digestive system. They are the pacemaker cells in gastrointestinal (GI) muscles. Absence, reduction in number or altered integrity of the ICC network may have a dramatic effect on GI system motility. More understanding of ICC physiology will foster advances in physiology of gut motility which will help in a future breakthrough in the pharmacological interventions to restore normal motor function of GI tract. This mini review describes what is known about the physiologic function and role of ICCs in GI system motility and in a variety of GI system motility disorders.展开更多
AIM:To examine whether acupuncture can prevent prolonged postoperative ileus(PPOI)after intraperitoneal surgery for colon cancer. METHODS:Ninety patients were recruited from the Fudan University Cancer Hospital,Shangh...AIM:To examine whether acupuncture can prevent prolonged postoperative ileus(PPOI)after intraperitoneal surgery for colon cancer. METHODS:Ninety patients were recruited from the Fudan University Cancer Hospital,Shanghai,China. After surgery,patients were randomized to receive acupuncture(once daily,starting on postoperative day 1, for up to six consecutive days)or usual care.PPOI was defined as an inability to pass flatus or have a bowel movement by 96 h after surgery.The main outcomes were time to first flatus,time to first bowel movement, and electrogastroenterography.Secondary outcomes were quality of life(QOL)measures,including pain, nausea,insomnia,abdominal distension/fullness,and sense of well-being. RESULTS:No significant differences in PPOI on day 4 (P=0.71)or QOL measures were found between the groups.There were also no group differences when the data were analyzed by examining those whose PPOI had resolved by day 5(P=0.69)or day 6(P= 0.88).No adverse events related to acupuncture were reported. CONCLUSION:Acupuncture did not prevent PPOI andwas not useful for treating PPOI once it had developed in this population.展开更多
BACKGROUND Functional dyspepsia(FD)is a common digestive disease with limited therapeutic options.According to evidence-based clinical practice,acupuncture or electroacupuncture(EA)seems to be a promising therapy for ...BACKGROUND Functional dyspepsia(FD)is a common digestive disease with limited therapeutic options.According to evidence-based clinical practice,acupuncture or electroacupuncture(EA)seems to be a promising therapy for patients with FD.However,there is still a lack of systematic reviews that have analyzed current clinical trials for a better understanding of mechanisms involved in the ameliorating effect of acupuncture and EA on FD.AIM To evaluate the results and qualities of existing clinical evidence for researching the underlying mechanisms of acupuncture/EA in treating FD.METHODS A systematic search of the literature was performed to identify randomized controlled trials in which research on the mechanism of acupuncture or EA was conducted in FD patients.Databases searched included PubMed,EMBASE,Cochrane Library,and Web of Science.Data extraction and quality assessment were completed by two investigators independently and the results of quality evaluation were exported through Review Manager V5.3.RESULTS Eight studies were included in this review with a total of 17 items for detecting techniques for mechanistic research.Positive effects of acupuncture and EA were observed in regulating gastric motility,gastric accommodation,mental status,gastrointestinal hormones,and central and autonomic functions while improving dyspeptic symptoms and quality of life.CONCLUSION The key findings of this systematic review support the potential of acupuncture and EA in altering the heterogeneous pathophysiology in patients with FD.However,high-quality studies with well-planned designs are necessary to provide more credible evidence.展开更多
OBJECTIVE:To evaluate the effects of electric acupoint stimulation on gastrointestinal hormones and motility among geriatric postoperative patients with gastrointestinal tumors,and to explore an efficient and noninvas...OBJECTIVE:To evaluate the effects of electric acupoint stimulation on gastrointestinal hormones and motility among geriatric postoperative patients with gastrointestinal tumors,and to explore an efficient and noninvasive method for postoperative recovery of bowel functions.METHODS:Forty patients were randomly and evenly assigned into a regular nursing care group(RNC) and an acupoint electric stimulation group(AES).Patients in the RNC group received regular nursing care and patients in the AES group received regular nursing care plus electric stimulation of acupoints.The serum levels of gastrin(GAS),motilin(MOT),and cholecystokinin(CCK),and an electrogastrogram(EGG) of all the patients were evaluated on the first,third,and fifth day after surgery.The time to first flatus after surgery and the number of patients with side effects such as abdominal pain,abdominal distention,and diarrhea were recorded.RESULTS:There were significant differences between the two groups in GAS,MOT,EGG,time to first flatus,abdominal pain,abdominal distention,and diarrhea(P < 0.05).CONCLUSION:Electric stimulation on acupoints could increase levels of GAS and MOT,promote the recovery of gastrointestinal functions,and decrease complications among postoperative senile patients with gastrointestinal tumors.展开更多
Constipation is a significant manifestation of a number of psychological disorders.Published papers recommend using self-assessment questionnaires for discriminating psychological from non-psychological constipated pa...Constipation is a significant manifestation of a number of psychological disorders.Published papers recommend using self-assessment questionnaires for discriminating psychological from non-psychological constipated patients before operating on them but reports from major surveys revealed that general practitioners failed to diagnose 70%of depressed patients using self-assessment questionnaires.Lower circulating concentrations of progesterone,17-hydroxyprogesterone,cortisol,testosterone,androstenedione,and dehydroepiandrostenedione sulfate(DHEAS) during the follicular phase in constipated young women compared with respective controls were found during the follicular phase of the menstrual cycles. During the luteal phase of the cycle,reductions were identified in estriol,cortisol and testosterone in the constipated group.Likewise,circulating concentrations of DHEAS were found to be lower in depressed patients than comparable healthy controls.DHEAS/cortisol ratios in morning serum and salivary samples were lower than those retrieved during other times of the day in depressed patients.The idea of recognizing major depression in constipated patients by measuring DHEAS/cortisol ratios in saliva and serum may be plausible but this possibility needs to be confirmed in well-designed studies.展开更多
Objective To investigate the effects of Da-Cheng-Qi Decoction(DCQD,大承气汤)combined with Lactobacillus acidophilus(LA)on the recovery of gastrointestinal(GI)function in traumatic brain-injured(TBI)mice.Methods A tota...Objective To investigate the effects of Da-Cheng-Qi Decoction(DCQD,大承气汤)combined with Lactobacillus acidophilus(LA)on the recovery of gastrointestinal(GI)function in traumatic brain-injured(TBI)mice.Methods A total of 150 male C57BL/6 mice were randomly divided into sham-injury,normal saline(NS),DCQD(0.4 mL/day),LA(⩾1×1010 cfu/day LA),DCQD+LA(LA administration at the same dosage after 4 h of feeding DCQD),and½DCQD+LA groups(LA administration at the same dosage after 4 h of feeding½DCQD dose)by a random number table,5–8 mice in each group.The sever TBI model was constructed according to Feeney’s enhanced gravitational forces of free falling.On days 1,3,and 7 post-TBI,plasma diamine oxidase(DAO)and D-lactic acid levels were assessed by enzyme-linked immunosorbent assay(ELISA).Occludin expression in the intestinal epithelium was assessed by Western blot analysis.Transmission electron microscopy(TEM)was used to observe the morphological changes in the network structure of interstitial cells of Cajal(ICC)and change of enteric nervous system-ICC-smooth muscle cell(ENS-ICC-SMC).Immunofluorescence staining was used to detect changes in the network structure of the ICC.Results Compared with the NS group,occludin expression in the DCQD+LA group significantly increased on Day 1,3,and 7 post-TBI(P<0.05 or P<0.01).The concentration of DAO significantly decreased in the LA,DCQD,and DCQD+LA groups on Day 3 and 7,whilst the D-lactate concentrations in the LA and½DCQD+LA groups decreased on Day 1 and 3 post-injury(P<0.05 or P<0.01).The NS group experienced a great damage on the ENS-ICC-SMC network morphology and ICC network structure,and all treatment groups had some improvements,among which the DCQD+LA group presented relatively intact network morphology.Conclusions DCQD combined with LA treatment could effectively repair the intestinal mucosal barrier and improve GI motility in mice after TBI.The combination of DCQD and LA was more effective than their respective monotherapies.展开更多
Objective: To investigate autophagy-related mechanisms of electroacupuncture(EA) action in improving gastrointestinal motility in mice with functional constipation(FC). Methods: According to a random number table, the...Objective: To investigate autophagy-related mechanisms of electroacupuncture(EA) action in improving gastrointestinal motility in mice with functional constipation(FC). Methods: According to a random number table, the Kunming mice were divided into the normal control, FC and EA groups in Experiment Ⅰ. The autophagy inhibitor 3-methyladenine(3-MA) was used to observe whether it antagonized the effects of EA in Experiment Ⅱ. An FC model was established by diphenoxylate gavage. Then the mice were treated with EA stimulation at Tianshu(ST 25) and Shangjuxu(ST 37) acupoints. The first black stool defecation time, the number, weight, and water content of 8-h feces, and intestinal transit rate were used to assess intestinal transit. Colonic tissues underwent histopathological assessment, and the expressions of autophagy markers microtubuleassociated protein 1 light chain 3(LC3) and Beclin-1 were detected by immunohistochemical staining. The expressions of phosphoinositide 3-kinases(PI3K)-protein kinase B(AKT)-mammalian target of rapamycin(m TOR) signaling pathway members were investigated by Western blot and quantitative reverse transcription-polymerase chain reaction, respectively. The relationship between enteric glial cells(EGCs) and autophagy was observed by confocal immunofluorescence microscopy, localization analysis, and electron microscopy. Results: EA treatment shortened the first black stool defecation time, increased the number, weight, and water content of 8-h feces, and improved the intestinal transit rate in FC mice(P<0.01). In terms of a putative autophagy mechanism, EA treatment promoted the expressions of LC3 and Beclin-1 proteins in the colonic tissue of FC mice(P<0.05), with glial fibrillary acidic protein(GFAP) and LC3 significantly colocalized. Furthermore, EA promoted colonic autophagy in FC mice by inhibiting PI3K/AKT/m TOR signaling(P<0.05 or P<0.01). The positive effect of EA on intestinal motility in FC mice was blocked by 3-MA. Conclusion: EA treatment can inhibit PI3K/AKT/m TOR signaling in the colonic tissues of FC mice, thereby promoting EGCs autophagy to improve intestinal motility.展开更多
Functional dyspepsia(FD)and gastroparesis are two common functional gastrointestinal diseases,affecting millions of people in both China and around the world.However,treatment options are limited and difficult due to ...Functional dyspepsia(FD)and gastroparesis are two common functional gastrointestinal diseases,affecting millions of people in both China and around the world.However,treatment options are limited and difficult due to multiple factors involved in their pathophysiologies.Therefore it is critically important to assess and understand pathophysiologies of these diseases before a treatment is given.In this minireview we provide updates on recent development in the pathophysiologies and treatments of FD and gastroparesis.In addition to common abnormalities in gastric accommodation,antral hypomotility and visceral hypersensitivity,FD has recently been reported to display a number of impairment in duodenal functions,such as low-grade inflammation and increased permeability.For gastroparesis,macrophages have recently been reported to play an important role in its pathophysiology.A brief overview on the assessment of pathophysiologies of FD and gastroparesis is also provided;pros and cons of clinically available methodologies as well as emerging methods are discussed.Finally,available treatment options for FD and gastroparesis are presented in this minireview,including authors′opinions on the advantages and disadvantages of each therapy.Potential novel therapies for FD and gastroparesis,especially those associated with Traditional Chinese Medicine,are also introduced and discussed.展开更多
Objective:To observe the impacts of electroacupuncture(EA)at auricular concha on gastrointestinal motility in the rats with type 2 diabetes(T2D).Methods:A total of 30 SD male rats were collected.8 rats of them were in...Objective:To observe the impacts of electroacupuncture(EA)at auricular concha on gastrointestinal motility in the rats with type 2 diabetes(T2D).Methods:A total of 30 SD male rats were collected.8 rats of them were in a control group and the rest22 rats were prepared as the model of T2D by intraperitoneal injection with streptozotocin(STZ)and high-fat forage.Blood glucose≥16.7 mmol/L for 3 times was considered as modeling success in rats,thus the rats were collected in a T2D group(n=16).These 16 T2D rats were divided into a model group(n=8)and an intradermal auricular electroacupuncture stimulation(iaES)group(n=8)according to random number table.In the iaES group,intervention with EA was applied at auricular concha unilaterally each time,once daily,on both sides alternatively,5-day treatment as 1 course,at the interval of 2 days between courses,totally for 4 weeks.No any intervention was given in the model group and the control group.At the end of 4 weeks of intervention,the gastric emptying and intestinal propulsion were determined,ELISA method was adopted to determine gastrin(GAS)and motilin(MTL),and random blood glucose value and food intake were recorded in the iaES group,the model group and the control group successively.Results:After modeling,as compared with the control group,the food intake and random blood glucose were increased significantly(both P<0.001)and body weight was reduced obviously(P<0.01)in the T2D group.After intervention for 4 weeks,compared with the model group,the food intake and gastric content weight were obviously reduced(both P<0.05),the serum GAS content,gastric emptying and intestinal transit rate,as well as random blood glucose reduction were all higher(all P<0.05)in the rats of the iaES group,but the difference was not significant in MTL between the iaES groups and the model group(P>0.05).Conclusion:Electroacupuncture at auricular concha improves gastrointestinal motility in T2D model rats.展开更多
OBJECTIVE:To investigate the influence of Qihuang decoction(芪黄煎剂)on enteric nervous system after gastrectomy in rats.METHODS:The morphology,distribution and number of intestinal neurons in enteric nervous system(E...OBJECTIVE:To investigate the influence of Qihuang decoction(芪黄煎剂)on enteric nervous system after gastrectomy in rats.METHODS:The morphology,distribution and number of intestinal neurons in enteric nervous system(ENS)were observed by immunofluorescence labeling and confocal laser scanning microscopy.Reverse transcriptionpolymerase chain reaction and Western blot were used to detect the mRNA and protein expression of intestinal neurotransmitters and corresponding receptors in ENS.RESULTS:The morphology and distribution of enteric neurons in ENS were changed after gastrectomy,and these neurons in Qihuang decoction group were similar with that of sham operation group.The number of ACh and SP positive neurons,mRNA and protein expression of excitatory neurotransmitters(ACh E,SP)and receptors(M3R,NK1R)were decreased after gastrectomy.And the intervention of Qihuang decoction could increase the number of ACh and SP positive neurons and promote the expression of their mRNA and protein.For vasoactive intestinal peptide(VIP)and nitric oxide synthase(NOS),the number of neurons and mRNA and protein expression of inhibitory neurotransmitters(VIP and NOS)and receptors(VIP2R)were increased after gastrectomy.And these rising indexes fall back after the intervention of Qihuang decoction.Besides,the intestinal propulsion rate in QH group was significantly increased than that in SEN and IEN group.CONCLUSIONS:These experimental results showed that after gastrectomy,early intervention with Qihuang decoction in small intestine will contribute to the postoperative recovery of enteric nervous system and intestinal propulsion rate,and consequently enhance gastrointestinal motility.展开更多
Objective To investigate the preliminary pharmacological screening of Cassia nomame.Methods The effect of aqueous extract from C.nomame on gastrointestinal motor function was investigated by assessing the intestinal t...Objective To investigate the preliminary pharmacological screening of Cassia nomame.Methods The effect of aqueous extract from C.nomame on gastrointestinal motor function was investigated by assessing the intestinal transit rate(ITR)of charcoal modeled into gastrointestinal motility dysfunction(GMD)by the administration of dopamine,atropine,or noradrenaline to the rats,respectively.Diuresis was studied in vivo by estimating the urine output.The anti-inflammatory activity was expressed as the percentage of swelling reduction by comparison on the mean thickness of ear swelling in mice.Results The ITR in these GMD animals was significantly retarded compared to that in normal animals.The retardation,however,was significantly inhibited by the ig administration of C.nomame(2 g/kg)for all GMD animals.The results suggested that C.nomame had the potential for development into a prokinetic agent that could prevent or alleviate GMD in patients.C.nomame increased urine output and suppressed significantly ear swelling induced by dirnethyl benzene in mice.Conclusion C.nomame could increase the gastrointestinal contractile activity of rats and has the effects of diuresis and anti-inflammation.展开更多
文摘Investigating gastrointestinal(GI)motility disorders relies on diagnostic tools to assess muscular contractions,peristalsis propagation and the integrity and coordination of various sphincters.Manometries are the gold standard to study the GI motor function but it is increasingly acknowledged that manometries do not provide a complete picture in relation to sphincters competencies and muscle fibrosis.Endolumenal functional lumen imaging probe(EndoFLIP)an emerging technology,uses impedance planimetry to measure hollow organs cross sectional area,distensibility and compliance.It has been successfully used as a complementary tool in the assessment of the upper and lower oesophageal sphincters,oesophageal body,the pylorus and the anal canal.In this article,we aim to review the uses of EndoFLIP as a tool to investigate GI motility disorders with a special focus on paediatric practice.The majority of EndoFLIP studies were conducted in adult patients but the uptake of the technology in paediatrics is increasing.EndoFLIP can provide a useful complementary data to the existing GI motility investigation in both children and adults.
基金the Natural Science Foundation of Shaanxi Province(No.2023-YBSF-086)Xi’an Science and Technology Plan Projects(No.21YXYJ0091)+1 种基金Key Projects of Shaanxi Provincial Department of Education(No.21JS041)Shaanxi Provincial People’s Hospital Science and Technology Talent Support Plan(No.2022BJ-05).
文摘Objective:To investigate the effect of auricular acupuncture on postoperative pain and gastrointestinal motility recovery after laparoscopic surgery for colorectal cancer.Methods:The clinical data of patients who underwent laparoscopic radical surgery for colorectal cancer in our hospital from April 2020 to December 2021 were collected.Based on the inclusion and exclusion criteria,76 patients were included in the retrospective analysis.Depending on whether they received auricular acupuncture or not,the patients were divided into two groups:46 patients in the experimental group(auricular acupuncture)and 30 patients in the control group.The differences between the two groups were analyzed.Results:The time to first flatus of the experimental group was significantly shorter than that of the control group(52.2±7.36 h versus 66.3±7.83 h;P<0.001).Similarly,the time to first defecation of the experimental group was significantly shorter than that of the control group(76.3±7.76 h versus 86.1±10.79 h;P<0.001).The time to first fluid intake of the auricular group was significantly shorter than that of the control group(90.4±8.92 h versus 107.3±9.66 h,P<0.001).Compared with the control group,the experimental group scored significantly lower on the visual analogue scale on postoperative days 2 and 3(P<0.001).Conclusion:Auricular acupuncture is an effective traditional Chinese medicine external treatment method.It can promote gastrointestinal motility recovery in patients after laparoscopic radical surgery for colorectal cancer and also reduce postoperative pain and discomfort.Furthermore,this therapy is easy to operate and well-accepted by patients.Therefore,it should be strongly promoted in clinical practice.
基金Supported by National Natural Science Foundation of China,No.81574071,No.81673883,and No.81873238Leading Talents of Traditional Chinese Medicine in Jiangsu Province(Second Batch),No.SLJ0225。
文摘Acupuncture has been used in China for thousands of years and has become more widely accepted by doctors and patients around the world. A large number of clinical studies and animal experiments have confirmed that acupuncture has a benign adjustment effect on gastrointestinal(GI) movement;however, the mechanism of this effect is unclear, especially in terms of neural mechanisms, and there are still many areas that require further exploration. This article reviews the recent data on the neural mechanism of acupuncture on GI movements. We summarize the neural mechanism of acupuncture on GI movement from four aspects: acupuncture signal transmission, the sympathetic and parasympathetic nervous system, the enteric nervous system, and the central nervous system.
基金Supported by The Fund of Scientific Research(FWO),Flanders, Project G.0134.07the University of Antwerp,BOF Grant FA02/3/3257
文摘AIM:To investigate the therapeutic effect of Schistosoma mansoni(S.mansoni) soluble worm proteins on gastrointestinal motility disturbances during experimental colitis in mice. METHODS:Colitis was induced by intrarectal injection of trinitrobenzene sulphate(TNBS) and 6 h later,mice were treated ip with S.mansoni proteins.Experiments were performed 5 d after TNBS injection.Inflammationwas quantified using validated inflammation parameters. Gastric emptying and geometric center were measured to assess in vivo gastrointestinal motility.Peristaltic activity of distal colonic segments was studied in vitro using a modified Trendelenburg set-up.Cytokine profiles of T-lymphocytes isolated from the colon were determined by real time reverse transcriptase-polymerase chain reaction. RESULTS:Intracolonic injection of TNBS caused severe colitis.Treatment with S.mansoni proteins significantly ameliorated colonic inflammation after 5 d.TNBS did not affect gastric emptying but significantly decreased the geometric center and impaired colonic peristaltic activity 5 d after the induction of colitis.Treatment with S.mansoni proteins ameliorated these in vivo and in vitro motility disturbances.In addition,TNBS injection caused a downregulation of effector T cell cytokines after 5 d,whereas a S.mansoni protein effect was no longer observed at this time point. CONCLUSION:Treatment with S.mansoni proteins attenuated intestinal inflammation and ameliorated motility disturbances during murine experimental colitis.
基金Supported by Fundacao de Amparo à Pesquisa do Estado de Sao Paulo(Fapesp),No.2010/14845-4 and No.2015/14923-9Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq),No.150456/2009-3
文摘AIM: To correlate gastric contractility, gastrointestinal transit, and hormone levels in non-pregnant(estrous cycle) and pregnant rats using noninvasive techniques. METHODS: Female rats(n = 23) were randomly divided into(1) non-pregnant,(contractility, n =6; transit, n = 6); and(2) pregnant(contractility, n = 5; transit, n = 6). In each estrous cycle phase or at 0, 7, 14, and 20 d after the confirmation of pregnancy, gastrointestinal transit was recorded by AC biosusceptometry(ACB), and gastric contractility was recorded by ACB and electromyography. After each recording, blood samples were obtained for progesterone and estradiol determination. RESULTS: In the estrous cycle, despite fluctuations of sex hormone levels, no significant changes in gastrointestinal motility were observed. Days 7 and 14 of pregnancy were characterized by significant changes in the frequency of contractions(3.90 ± 0.42 cpm and 3.60 ± 0.36 cpm vs 4.33 ± 0.25 cpm) and gastric emptying(168 ± 17 min and 165 ± 15 min vs 113 ± 15 min) compared with day 0. On these same days, progesterone levels significantly increased compared with control(54.23 ± 15.14 ng/m L and 129.96 ± 30.52 ng/mL vs 13.25 ± 6.31 ng/mL). On day 14, we observed the highest level of progesterone and the lowest level of estradiol compared with day 0(44.3 ± 15.18 pg/mL vs 24.96 ± 5.96 pg/mL). CONCLUSION: Gastrointestinal motility was unaffected by the estrous cycle. In our data, high progesterone and low estradiol levels can be associated with decreased contraction frequency and slow gastric emptying.
基金“Double first-class”construction project of Mongolian medicine scientific research and innovation team fund(190301)International cooperative scientific and technological innovation project of Mongolian medicine standardization research(MDK2018009)+1 种基金2018 National Civil Affairs Commission-Ministry of Education Mongolian Medicine R&D Engineering Key Laboratory Open Project(MDK2018056)Mongolian Medicine R&D National and Local Joint Engineering Research Center Open Fund Project(MDK2019044).
文摘Functional dyspepsia(FD)is a regularly diagnosed clinical gastrointestinal ailment with a high incidence rate that can considerably impact patients’health and quality of life and impose a substantial financial burden.Modern research on the pathophysiology of functional dyspepsia has not thoroughly explained the underlying reasons.The condition does not manifest any significant organ abnormalities,which raises the disease’s difficulty coefficient.Major pathogenic exceptions in FD include gastrointestinal motor dysfunction,gastrointestinal hormone secretion problem,visceral hypersensitivity,and brain-gut axis.Several ion channels have reportedly been implicated in the pathophysiological process of FD.Therefore,it is crucial to comprehend the probable activities of various ion channels in FD.This study focuses on the current state of research on the possible role of several ion channels in the pathogenesis of FD.
文摘Gastric dyskinesis functional dyspepsia is a commonly clinical condition characterized as epigastric discomfort,distension after meals,early satiety and belching,which are commonly associated with the dysfunction of gastrointestinal motility.
文摘Background: Previous studies on fasting and gastrointestinal motility were reported with information lacking concerning prolonged continuous fasting and gastrointestinal motility. This study investigated the effect of prolonged fasting duration on gastrointestinal motility. Methods: Forty-five (45) male Wistar rats, with body weights between 180 - 200 g were used. They were randomly assigned into three (3) groups. Group1: control (rats fasted for 18 h—common duration of fasting for motility studies), groups 2 and 3 fasted for 48 and 72 h respectively. Five (5) rats per experiment and per group were considered. Blood glucose was determined by glucose oxidase method, gastric emptying was assessed by hydrated carbohydrate meal, intestinal motility by charcoal meal, and colonic motility was assessed using bead test. Data were reported in Mean ± SEM and analyzed with one-way ANOVA. Differences in results were considered significant at p ≤ 0.05. Results: There was no significant change in the blood glucose level (mmol/L) of rats in the 48 h group (2.94 ± 0.35) and 72 h group (3.20 ± 0.32) as compared with the control (3.62 ± 0.19). There was a significant decrease in the rate of gastric emptying (g) in the 72 h group (0.20 ± 0.08) compared with the control (0.64 ± 0.16). The intestinal transit (cm) in the 48 h group (67.54 ± 6.15) and 72 h group (72.10 ± 7.60) increased significantly when compared with the control (42.14 ± 3.14). There was a significant decrease in the colonic motility time (Sec.) in the 48 h group (2707 ± 864.1) and 72 h group (6363 ± 968.1) when compared with the control (263.8 ± 64.26). Conclusion: Extended fasting durations decrease the rate of gastric emptying and colonic motility. It suggests that extended fasting durations could be beneficial in intestinal spasms or where the gut is required to relax.
文摘Interstitial cells of Cajal (ICC) are important players in the symphony of gut motility. They have a very signif icant physiological role orchestrating the normal peristaltic activity of the digestive system. They are the pacemaker cells in gastrointestinal (GI) muscles. Absence, reduction in number or altered integrity of the ICC network may have a dramatic effect on GI system motility. More understanding of ICC physiology will foster advances in physiology of gut motility which will help in a future breakthrough in the pharmacological interventions to restore normal motor function of GI tract. This mini review describes what is known about the physiologic function and role of ICCs in GI system motility and in a variety of GI system motility disorders.
基金Supported by US National Cancer Institute (NCI) grants CA108084 and CA12153031
文摘AIM:To examine whether acupuncture can prevent prolonged postoperative ileus(PPOI)after intraperitoneal surgery for colon cancer. METHODS:Ninety patients were recruited from the Fudan University Cancer Hospital,Shanghai,China. After surgery,patients were randomized to receive acupuncture(once daily,starting on postoperative day 1, for up to six consecutive days)or usual care.PPOI was defined as an inability to pass flatus or have a bowel movement by 96 h after surgery.The main outcomes were time to first flatus,time to first bowel movement, and electrogastroenterography.Secondary outcomes were quality of life(QOL)measures,including pain, nausea,insomnia,abdominal distension/fullness,and sense of well-being. RESULTS:No significant differences in PPOI on day 4 (P=0.71)or QOL measures were found between the groups.There were also no group differences when the data were analyzed by examining those whose PPOI had resolved by day 5(P=0.69)or day 6(P= 0.88).No adverse events related to acupuncture were reported. CONCLUSION:Acupuncture did not prevent PPOI andwas not useful for treating PPOI once it had developed in this population.
基金Supported by the National Natural Science Foundation of China,No.81703923 and No.81820108033.
文摘BACKGROUND Functional dyspepsia(FD)is a common digestive disease with limited therapeutic options.According to evidence-based clinical practice,acupuncture or electroacupuncture(EA)seems to be a promising therapy for patients with FD.However,there is still a lack of systematic reviews that have analyzed current clinical trials for a better understanding of mechanisms involved in the ameliorating effect of acupuncture and EA on FD.AIM To evaluate the results and qualities of existing clinical evidence for researching the underlying mechanisms of acupuncture/EA in treating FD.METHODS A systematic search of the literature was performed to identify randomized controlled trials in which research on the mechanism of acupuncture or EA was conducted in FD patients.Databases searched included PubMed,EMBASE,Cochrane Library,and Web of Science.Data extraction and quality assessment were completed by two investigators independently and the results of quality evaluation were exported through Review Manager V5.3.RESULTS Eight studies were included in this review with a total of 17 items for detecting techniques for mechanistic research.Positive effects of acupuncture and EA were observed in regulating gastric motility,gastric accommodation,mental status,gastrointestinal hormones,and central and autonomic functions while improving dyspeptic symptoms and quality of life.CONCLUSION The key findings of this systematic review support the potential of acupuncture and EA in altering the heterogeneous pathophysiology in patients with FD.However,high-quality studies with well-planned designs are necessary to provide more credible evidence.
基金Supported by the National Natural Science Foundation Project of China(Transcutaneous Electrical Acupoint Stimulation Prevents the Deep Vein Thrombosis:the Role of Endothelium Modulation,No.81202750)
文摘OBJECTIVE:To evaluate the effects of electric acupoint stimulation on gastrointestinal hormones and motility among geriatric postoperative patients with gastrointestinal tumors,and to explore an efficient and noninvasive method for postoperative recovery of bowel functions.METHODS:Forty patients were randomly and evenly assigned into a regular nursing care group(RNC) and an acupoint electric stimulation group(AES).Patients in the RNC group received regular nursing care and patients in the AES group received regular nursing care plus electric stimulation of acupoints.The serum levels of gastrin(GAS),motilin(MOT),and cholecystokinin(CCK),and an electrogastrogram(EGG) of all the patients were evaluated on the first,third,and fifth day after surgery.The time to first flatus after surgery and the number of patients with side effects such as abdominal pain,abdominal distention,and diarrhea were recorded.RESULTS:There were significant differences between the two groups in GAS,MOT,EGG,time to first flatus,abdominal pain,abdominal distention,and diarrhea(P < 0.05).CONCLUSION:Electric stimulation on acupoints could increase levels of GAS and MOT,promote the recovery of gastrointestinal functions,and decrease complications among postoperative senile patients with gastrointestinal tumors.
文摘Constipation is a significant manifestation of a number of psychological disorders.Published papers recommend using self-assessment questionnaires for discriminating psychological from non-psychological constipated patients before operating on them but reports from major surveys revealed that general practitioners failed to diagnose 70%of depressed patients using self-assessment questionnaires.Lower circulating concentrations of progesterone,17-hydroxyprogesterone,cortisol,testosterone,androstenedione,and dehydroepiandrostenedione sulfate(DHEAS) during the follicular phase in constipated young women compared with respective controls were found during the follicular phase of the menstrual cycles. During the luteal phase of the cycle,reductions were identified in estriol,cortisol and testosterone in the constipated group.Likewise,circulating concentrations of DHEAS were found to be lower in depressed patients than comparable healthy controls.DHEAS/cortisol ratios in morning serum and salivary samples were lower than those retrieved during other times of the day in depressed patients.The idea of recognizing major depression in constipated patients by measuring DHEAS/cortisol ratios in saliva and serum may be plausible but this possibility needs to be confirmed in well-designed studies.
基金Supported by The General Project of Traditional Chinese Medici ne Tech no logical Projects for the Health Bureau of Chongqing(No.2012-2-91)。
文摘Objective To investigate the effects of Da-Cheng-Qi Decoction(DCQD,大承气汤)combined with Lactobacillus acidophilus(LA)on the recovery of gastrointestinal(GI)function in traumatic brain-injured(TBI)mice.Methods A total of 150 male C57BL/6 mice were randomly divided into sham-injury,normal saline(NS),DCQD(0.4 mL/day),LA(⩾1×1010 cfu/day LA),DCQD+LA(LA administration at the same dosage after 4 h of feeding DCQD),and½DCQD+LA groups(LA administration at the same dosage after 4 h of feeding½DCQD dose)by a random number table,5–8 mice in each group.The sever TBI model was constructed according to Feeney’s enhanced gravitational forces of free falling.On days 1,3,and 7 post-TBI,plasma diamine oxidase(DAO)and D-lactic acid levels were assessed by enzyme-linked immunosorbent assay(ELISA).Occludin expression in the intestinal epithelium was assessed by Western blot analysis.Transmission electron microscopy(TEM)was used to observe the morphological changes in the network structure of interstitial cells of Cajal(ICC)and change of enteric nervous system-ICC-smooth muscle cell(ENS-ICC-SMC).Immunofluorescence staining was used to detect changes in the network structure of the ICC.Results Compared with the NS group,occludin expression in the DCQD+LA group significantly increased on Day 1,3,and 7 post-TBI(P<0.05 or P<0.01).The concentration of DAO significantly decreased in the LA,DCQD,and DCQD+LA groups on Day 3 and 7,whilst the D-lactate concentrations in the LA and½DCQD+LA groups decreased on Day 1 and 3 post-injury(P<0.05 or P<0.01).The NS group experienced a great damage on the ENS-ICC-SMC network morphology and ICC network structure,and all treatment groups had some improvements,among which the DCQD+LA group presented relatively intact network morphology.Conclusions DCQD combined with LA treatment could effectively repair the intestinal mucosal barrier and improve GI motility in mice after TBI.The combination of DCQD and LA was more effective than their respective monotherapies.
基金Supported by the National Natural Science Foundation of China (No.82074554)the Foundation of the Science and Technology Department of Sichuan Province,China (No.2021YJ0197)Chengdu University of Traditional Chinese Medicine Xinglin Scholars Program (No.YXRC2018007)。
文摘Objective: To investigate autophagy-related mechanisms of electroacupuncture(EA) action in improving gastrointestinal motility in mice with functional constipation(FC). Methods: According to a random number table, the Kunming mice were divided into the normal control, FC and EA groups in Experiment Ⅰ. The autophagy inhibitor 3-methyladenine(3-MA) was used to observe whether it antagonized the effects of EA in Experiment Ⅱ. An FC model was established by diphenoxylate gavage. Then the mice were treated with EA stimulation at Tianshu(ST 25) and Shangjuxu(ST 37) acupoints. The first black stool defecation time, the number, weight, and water content of 8-h feces, and intestinal transit rate were used to assess intestinal transit. Colonic tissues underwent histopathological assessment, and the expressions of autophagy markers microtubuleassociated protein 1 light chain 3(LC3) and Beclin-1 were detected by immunohistochemical staining. The expressions of phosphoinositide 3-kinases(PI3K)-protein kinase B(AKT)-mammalian target of rapamycin(m TOR) signaling pathway members were investigated by Western blot and quantitative reverse transcription-polymerase chain reaction, respectively. The relationship between enteric glial cells(EGCs) and autophagy was observed by confocal immunofluorescence microscopy, localization analysis, and electron microscopy. Results: EA treatment shortened the first black stool defecation time, increased the number, weight, and water content of 8-h feces, and improved the intestinal transit rate in FC mice(P<0.01). In terms of a putative autophagy mechanism, EA treatment promoted the expressions of LC3 and Beclin-1 proteins in the colonic tissue of FC mice(P<0.05), with glial fibrillary acidic protein(GFAP) and LC3 significantly colocalized. Furthermore, EA promoted colonic autophagy in FC mice by inhibiting PI3K/AKT/m TOR signaling(P<0.05 or P<0.01). The positive effect of EA on intestinal motility in FC mice was blocked by 3-MA. Conclusion: EA treatment can inhibit PI3K/AKT/m TOR signaling in the colonic tissues of FC mice, thereby promoting EGCs autophagy to improve intestinal motility.
文摘Functional dyspepsia(FD)and gastroparesis are two common functional gastrointestinal diseases,affecting millions of people in both China and around the world.However,treatment options are limited and difficult due to multiple factors involved in their pathophysiologies.Therefore it is critically important to assess and understand pathophysiologies of these diseases before a treatment is given.In this minireview we provide updates on recent development in the pathophysiologies and treatments of FD and gastroparesis.In addition to common abnormalities in gastric accommodation,antral hypomotility and visceral hypersensitivity,FD has recently been reported to display a number of impairment in duodenal functions,such as low-grade inflammation and increased permeability.For gastroparesis,macrophages have recently been reported to play an important role in its pathophysiology.A brief overview on the assessment of pathophysiologies of FD and gastroparesis is also provided;pros and cons of clinically available methodologies as well as emerging methods are discussed.Finally,available treatment options for FD and gastroparesis are presented in this minireview,including authors′opinions on the advantages and disadvantages of each therapy.Potential novel therapies for FD and gastroparesis,especially those associated with Traditional Chinese Medicine,are also introduced and discussed.
基金Supported by Tradition Chinese Medicine Science Technology Project of Jiangsu Province:YB201808Postgraduate Practice Innovation Program of Jiangsu Province:KYCX20_1544。
文摘Objective:To observe the impacts of electroacupuncture(EA)at auricular concha on gastrointestinal motility in the rats with type 2 diabetes(T2D).Methods:A total of 30 SD male rats were collected.8 rats of them were in a control group and the rest22 rats were prepared as the model of T2D by intraperitoneal injection with streptozotocin(STZ)and high-fat forage.Blood glucose≥16.7 mmol/L for 3 times was considered as modeling success in rats,thus the rats were collected in a T2D group(n=16).These 16 T2D rats were divided into a model group(n=8)and an intradermal auricular electroacupuncture stimulation(iaES)group(n=8)according to random number table.In the iaES group,intervention with EA was applied at auricular concha unilaterally each time,once daily,on both sides alternatively,5-day treatment as 1 course,at the interval of 2 days between courses,totally for 4 weeks.No any intervention was given in the model group and the control group.At the end of 4 weeks of intervention,the gastric emptying and intestinal propulsion were determined,ELISA method was adopted to determine gastrin(GAS)and motilin(MTL),and random blood glucose value and food intake were recorded in the iaES group,the model group and the control group successively.Results:After modeling,as compared with the control group,the food intake and random blood glucose were increased significantly(both P<0.001)and body weight was reduced obviously(P<0.01)in the T2D group.After intervention for 4 weeks,compared with the model group,the food intake and gastric content weight were obviously reduced(both P<0.05),the serum GAS content,gastric emptying and intestinal transit rate,as well as random blood glucose reduction were all higher(all P<0.05)in the rats of the iaES group,but the difference was not significant in MTL between the iaES groups and the model group(P>0.05).Conclusion:Electroacupuncture at auricular concha improves gastrointestinal motility in T2D model rats.
基金Supported by Clinical Scientific Research Project of the First Affiliated Hospital of Anhui University of Chinese Medicine:to Investigate the Effect of Qihuang Decoction on Gastrointestinal Function and its Molecular Mechanism in Rats after Gastrectomy based on ENS-ICC-SMC Network Architecture(2020yfyzc02)the National Natural Science Foundation of China:the Effect of Qihuang Decoction on Intestinal Lymphocyte Homing after Gastrectomy in Rats(No.81403406),the National Natural Science Foundation of China:Qihuang Decoction Inhibits Bacterial Migration during Gastrectomy in Rats and Regulates the M1-type Polarization of Macrophages induced by TLR4/NF-κB Signaling Pathway based on lncRNA MALAT1 Sponge Mir-146a(No.82174160)+1 种基金Supporting Program for Excellent Young Talents in Colleges and Universities of Anhui Province:the Effects of Qihuang Decoction on Gastrointestinal Motility in Rats after Gastrectomy and its Molecular Mechanism were Studied based on ENS Framework(gxyq2019035)General projects of Anhui Natural Science Foundation:to Investigate the Inhibition of Cryptotanshinone on 5-FUSGC-7901 Human Gastric Cancer Cells and its Mechanism based on JAK2/STAT3 Signaling Pathway(2008085MH266)
文摘OBJECTIVE:To investigate the influence of Qihuang decoction(芪黄煎剂)on enteric nervous system after gastrectomy in rats.METHODS:The morphology,distribution and number of intestinal neurons in enteric nervous system(ENS)were observed by immunofluorescence labeling and confocal laser scanning microscopy.Reverse transcriptionpolymerase chain reaction and Western blot were used to detect the mRNA and protein expression of intestinal neurotransmitters and corresponding receptors in ENS.RESULTS:The morphology and distribution of enteric neurons in ENS were changed after gastrectomy,and these neurons in Qihuang decoction group were similar with that of sham operation group.The number of ACh and SP positive neurons,mRNA and protein expression of excitatory neurotransmitters(ACh E,SP)and receptors(M3R,NK1R)were decreased after gastrectomy.And the intervention of Qihuang decoction could increase the number of ACh and SP positive neurons and promote the expression of their mRNA and protein.For vasoactive intestinal peptide(VIP)and nitric oxide synthase(NOS),the number of neurons and mRNA and protein expression of inhibitory neurotransmitters(VIP and NOS)and receptors(VIP2R)were increased after gastrectomy.And these rising indexes fall back after the intervention of Qihuang decoction.Besides,the intestinal propulsion rate in QH group was significantly increased than that in SEN and IEN group.CONCLUSIONS:These experimental results showed that after gastrectomy,early intervention with Qihuang decoction in small intestine will contribute to the postoperative recovery of enteric nervous system and intestinal propulsion rate,and consequently enhance gastrointestinal motility.
文摘Objective To investigate the preliminary pharmacological screening of Cassia nomame.Methods The effect of aqueous extract from C.nomame on gastrointestinal motor function was investigated by assessing the intestinal transit rate(ITR)of charcoal modeled into gastrointestinal motility dysfunction(GMD)by the administration of dopamine,atropine,or noradrenaline to the rats,respectively.Diuresis was studied in vivo by estimating the urine output.The anti-inflammatory activity was expressed as the percentage of swelling reduction by comparison on the mean thickness of ear swelling in mice.Results The ITR in these GMD animals was significantly retarded compared to that in normal animals.The retardation,however,was significantly inhibited by the ig administration of C.nomame(2 g/kg)for all GMD animals.The results suggested that C.nomame had the potential for development into a prokinetic agent that could prevent or alleviate GMD in patients.C.nomame increased urine output and suppressed significantly ear swelling induced by dirnethyl benzene in mice.Conclusion C.nomame could increase the gastrointestinal contractile activity of rats and has the effects of diuresis and anti-inflammation.
