Inflammatory bowel disease(IBD)is a chronic,immune-mediated inflammatory disease characterized by the destruction of the structure and function of the intestinal epithelial barrier.Due to the poor remission effect and...Inflammatory bowel disease(IBD)is a chronic,immune-mediated inflammatory disease characterized by the destruction of the structure and function of the intestinal epithelial barrier.Due to the poor remission effect and severe adverse events associated with current clinical medications,IBD remains an incurable disease.Here,we demonstrated a novel treatment strategy with high safety and effective inflammation remission via tissue-adhesive molecular coating.The molecular coating is composed of o-nitrobenzaldehyde(NB)-modified Gelatin(GelNB),which can strongly bond with-NH_(2)on the intestinal surface of tissue to form a thin biophysical barrier.We found that this molecular coating was able to stay on the surface of the intestine for long periods of time,effectively protecting the damaged intestinal epithelium from irritations of external intestinal metabolites and harmful flora.In addition,our results showed that this coating not only provided a beneficial environment for cell migration and proliferation to promote intestinal repair and regeneration,but also achieved a better outcome of IBD by reducing intestinal inflammation.Moreover,the in vivo experiments showed that the GelNB was better than the classic clinical medication-mesalazine.Therefore,our molecular coating showed potential as a promising strategy for the prevention and treatment of IBD.展开更多
Corneal injuries will cause corneal surface diseases that may lead to blindness in millions of people worldwide.There is a tremendous need for biomaterials that can promote corneal regeneration with practical feasibil...Corneal injuries will cause corneal surface diseases that may lead to blindness in millions of people worldwide.There is a tremendous need for biomaterials that can promote corneal regeneration with practical feasibility.Here we demonstrate a strategy of a protein coating for corneal injury regeneration.We synthesize an o-nitrosobenzaldehyde group(NB)-modified gelatin(GelNB),which could adhere directly to the corneal surface with covalent bonding to form a thin molecular coating.The molecular coating could avoid rapid clearance and provide a favorable environment for cell migration,thereby effectively accelerating corneal repair and regeneration.The histological structure of the regenerated cornea is more similar to the native cornea.This molecular coating can be used conveniently as an eye drop solution,which makes it a promising strategy for corneal regeneration.展开更多
文摘Inflammatory bowel disease(IBD)is a chronic,immune-mediated inflammatory disease characterized by the destruction of the structure and function of the intestinal epithelial barrier.Due to the poor remission effect and severe adverse events associated with current clinical medications,IBD remains an incurable disease.Here,we demonstrated a novel treatment strategy with high safety and effective inflammation remission via tissue-adhesive molecular coating.The molecular coating is composed of o-nitrobenzaldehyde(NB)-modified Gelatin(GelNB),which can strongly bond with-NH_(2)on the intestinal surface of tissue to form a thin biophysical barrier.We found that this molecular coating was able to stay on the surface of the intestine for long periods of time,effectively protecting the damaged intestinal epithelium from irritations of external intestinal metabolites and harmful flora.In addition,our results showed that this coating not only provided a beneficial environment for cell migration and proliferation to promote intestinal repair and regeneration,but also achieved a better outcome of IBD by reducing intestinal inflammation.Moreover,the in vivo experiments showed that the GelNB was better than the classic clinical medication-mesalazine.Therefore,our molecular coating showed potential as a promising strategy for the prevention and treatment of IBD.
基金This work was supported by the National Key R&D Program of China(2017YFA0104900)National Natural Science Foundation of China(31830029,32000957,81972053).
文摘Corneal injuries will cause corneal surface diseases that may lead to blindness in millions of people worldwide.There is a tremendous need for biomaterials that can promote corneal regeneration with practical feasibility.Here we demonstrate a strategy of a protein coating for corneal injury regeneration.We synthesize an o-nitrosobenzaldehyde group(NB)-modified gelatin(GelNB),which could adhere directly to the corneal surface with covalent bonding to form a thin molecular coating.The molecular coating could avoid rapid clearance and provide a favorable environment for cell migration,thereby effectively accelerating corneal repair and regeneration.The histological structure of the regenerated cornea is more similar to the native cornea.This molecular coating can be used conveniently as an eye drop solution,which makes it a promising strategy for corneal regeneration.
