Human papillomaviruses (HPVs) are well known for being linked to the development of cervical cancers, most of them being caused by the high-risk (HR) oncogenic genotypes, mainly 16 and 18. The efficacy of 2LPAPI<su...Human papillomaviruses (HPVs) are well known for being linked to the development of cervical cancers, most of them being caused by the high-risk (HR) oncogenic genotypes, mainly 16 and 18. The efficacy of 2LPAPI<sup><sup>®</sup> </sup>(Labo’Life), a micro-immunotherapy homeopathic drug, has been evaluated in HR-HPV infected women (n = 18), in a private gynecology practice, by comparing them to an untreated control group (n = 18). Patients were 20 to 45 years old and had cytology with Atypical Squamous Cells of Undetermined Significance (ASCUS) or Low grade Superficial Intra Lesions/ Cervical Intraepithelial Neoplasia Grade I (LSIL/CINI). Patients freely chose to be treated with the drug or not. Those deciding not to take the drug remained untreated and were followed as a control group. The drug was taken at the regimen of one capsule per day during 6 months. HR-HPV and cytology were evaluated at 6 and 12 months. After 12 months, HR-HPV was cleared in 78% of the patients taking the drug versus 44% in those not taking it (p = 0.086). In patients over 25 years, HR-HPV clearance in the treated group was significantly higher (81.3%) than in the control group (20%) (p = 0.004). The difference in the regression of the lesion grades almost reached statistical significance (p = 0.053). This follow-up confirms that the micro-immunotherapy drug 2LPAPI<sup><sup>®</sup></sup> is a safe and effective therapeutic approach to treat HR-HPV cervical lesions in women over 25 years.展开更多
Bovine Herpesvirus-1 (BoHV-1) is distributed worldwide and is a major pathogen in cattle, being the causal agent of a variety of clinical syndromes. The aim of this study was to isolate and to characterize (molecular ...Bovine Herpesvirus-1 (BoHV-1) is distributed worldwide and is a major pathogen in cattle, being the causal agent of a variety of clinical syndromes. The aim of this study was to isolate and to characterize (molecular and biological characterization) BoHV-1 from 29 immunosuppressed animals. It was possible to obtain 18 isolates, each from a different animal, such as from the respiratory and reproductive tracts. In some cases the cytopathic effect was visible 12 hours post-inoculation, and became characteristic after 36-48 hours. Biological characteristics were evaluated and compared with Iowa and Colorado-1 reference strains, and differences were found in plaque size, virus titer measured by TCID50 and PFU/mL, and one step virus curves. These results showed that some isolates had a highly virulent-like behavior in vitro, compared to the reference strains, with shorter eclipse periods, faster release of virus into the supernatants, and higher burst size and viral titer. There were no differences in glycoprotein expression of BoHV-1 isolates, measured by Western blot on monolayers. Moreover, using restriction endonucleases analysis, most of the viruses were confirmed as BoHV-1.1 and just one of them was confirmed as BoHV-1.2a subtype. These findings suggest that some wild-type BoHV-1 isolates could be useful as seeds to develop new monovalent vaccines.展开更多
Aim： To evaluate the association between selected potential socio-demographic and behavioral risk factors and infertility in Nigerian men. Methods： There were two groups in this study. One group consisted of 150 men...Aim： To evaluate the association between selected potential socio-demographic and behavioral risk factors and infertility in Nigerian men. Methods： There were two groups in this study. One group consisted of 150 men with proven male infertility, and the other consisted of 150 fertile men with normal semen parameters. Both were matched for age, place of residence and key socio-demographic variables. They were compared for sexual history, past medical and surgical history, past exposures to sexually transmitted infections and treatment, past and current use of drugs as well as smoking and alcohol intake history. Results： Infertile men were significantly more likely than fertile men to report having experienced penile discharge, painful micturition and genital ulcers, less likely to seek treatment for these symptoms and more likely to seek treatment with informal sector providers. Multivariate analysis showed that male infertility was significantly associated with bacteria in semen cultures, self-reporting of previous use of traditional medications and moderate to heavy alcohol intake, but not with smoking and occupational types. Conclusion： Infertility is associated with various proxies of sexually transmitted infections （STIs） and poor healthcare-seeking behavior for STIs in Nigerian men.展开更多
Inhibitors of sodium-glucose co-transporter type 2(SGLT2), such as canagliflozin and dapagliflozin, are recently approved for treatment of type 2 diabetes. These agents lower blood glucose mainly by increasing urinary...Inhibitors of sodium-glucose co-transporter type 2(SGLT2), such as canagliflozin and dapagliflozin, are recently approved for treatment of type 2 diabetes. These agents lower blood glucose mainly by increasing urinary glucose excretion. Compared with placebo, SGLT2 inhibitors reduce hemoglobin A1c(Hb A1c) levels by an average of 0.5%-0.8% when used as monotherapy or add-on therapy. Advantages of this drug class include modest weight loss of approximately 2 kg, low risk of hypoglycemia, and decrease blood pressure of approximately 4 mm Hg systolic and 2 mm Hg diastolic. These characteristics make these agents potential add-on therapy in patients with Hb A1 c levels close to 7%-8.0%, particularly if these patients are obese, hypertensive, and/or prone for hypoglycemia. Meanwhile, these drugs are limited by high frequency of genital mycotic infections. Less common adverse effects include urinary tract infections, hypotension, dizziness, and worsening renal function. SGLT2 inhibitors should be used with caution in the elderly because of increased adverse effects, and should not be used in chronic kidney disease due to decreased or lack of efficacy and nephrotoxicity. Overall, SGLT2 inhibitors are useful addition for treatment of select groups of patients with type 2 diabetes,but their efficacy and safety need to be established in long-term clinical trials.展开更多
The aims of this study were （a） to determine the prevalence of subjects with semen hyperviscosity （SHV） in a large population of male partners of subfertile couples; （b） to identify any correlation between SHV a...The aims of this study were （a） to determine the prevalence of subjects with semen hyperviscosity （SHV） in a large population of male partners of subfertile couples; （b） to identify any correlation between SHV and infections or inflammation of the genital tract; （c） to assess the effects of therapeutic approaches for treating SHV; and （d） to assess sperm kinetic parameters after successful treatment of SHV. A retrospective study of 1 833 male partners of subfertile couples was conducted. Next, clinical, seminal, bacteriological and ultrasound studies involving 52 subjects suffering from SHV were performed, and the SHV was classified as being mild （length of thread 〉 2 cm and ≤4 cm）, moderate （〉 4 cm and ≤ 6 cm） or severe （〉 6 cm）. The prevalence of SHV was observed in 26.2% （480） of the subjects, with 13.2% suffering from mild, 6.6% from moderate and 6.4% from severe SHV. Treatment was completely successful in only 27 subjects （52.0%）, primarily in those who had mild basal SHV with a positive semen culture. In these subjects, progressive motility percentage, straight line velocity and linearity were significantly higher than pre-treatment levels. SHV is often found in subjects with subfertility. Pathogenesis was strictly related to infective/inflammatory factors in only 48.0% of cases; therefore, it is possible that biochemical, enzymatic or genetic factors have a role in this condition.展开更多
Objective:Genital chlamydial infection is a globally common sexually transmitted infection caused by Chlamydia trachomatis.This study aimed to describe the epidemiological characteristics of the disease reported in Ch...Objective:Genital chlamydial infection is a globally common sexually transmitted infection caused by Chlamydia trachomatis.This study aimed to describe the epidemiological characteristics of the disease reported in China in 2019.Methods:Genital chlamydial infection cases were identified in 105 national sexually transmitted disease surveillance sites from the National Notifiable Infectious Disease Reporting System,which covers 31 provinces,autonomous regions,and municipalities in Chinese mainland.Data were collected and used to calculate the overall incidence,and the incidences per surveillance site,province,sex,and age group.Results:In 2019,there were 50,874 new cases of genital chlamydial infection reported in China,corresponding to an incidence of 55.32 cases per 100,000 population,and comprising a 9.98%increase from 2018(50.30 cases per 100,000 population).The highest incidences of genital chlamydial infection occurred in Zhejiang,Guangdong province,Guangxi autonomous region,and Hainan province(average 137.49-233.37 cases per 100,000 population).The incidence of genital chlamydial infection was much higher in females than males,with a female-to-male ratio of 3.09(84.55 per 100,000 population vs 27.35 per 100,000 population).The highest incidence of genital chlamydial infection was reported in the 20-24-year age group(184.70 cases per 100,000 population),followed by the 25-29-year age group(180.79 cases per 100,000)and 30-34-year age group(151.53 cases per 100,000 population).The highest proportion(68.30%)of genital chlamydial infection was reported from general hospitals,followed by maternal and child health hospitals(25.59%).Conclusion:The incidence rate of genital chlamydial infection in 2019 has an increase trend from 2018 and be a common sexually transmitted infection in China.The incidence is much higher in females than in males.Sexually active young adults are the highest risk population.There is substantial geographic diversity in incidence,with the highest incidence occurring in the southeast coastal areas with a developed economy.Epidemic of genital chlamydial infection in China should be concerned,and its surveillance needs to be improved.展开更多
文摘Human papillomaviruses (HPVs) are well known for being linked to the development of cervical cancers, most of them being caused by the high-risk (HR) oncogenic genotypes, mainly 16 and 18. The efficacy of 2LPAPI<sup><sup>®</sup> </sup>(Labo’Life), a micro-immunotherapy homeopathic drug, has been evaluated in HR-HPV infected women (n = 18), in a private gynecology practice, by comparing them to an untreated control group (n = 18). Patients were 20 to 45 years old and had cytology with Atypical Squamous Cells of Undetermined Significance (ASCUS) or Low grade Superficial Intra Lesions/ Cervical Intraepithelial Neoplasia Grade I (LSIL/CINI). Patients freely chose to be treated with the drug or not. Those deciding not to take the drug remained untreated and were followed as a control group. The drug was taken at the regimen of one capsule per day during 6 months. HR-HPV and cytology were evaluated at 6 and 12 months. After 12 months, HR-HPV was cleared in 78% of the patients taking the drug versus 44% in those not taking it (p = 0.086). In patients over 25 years, HR-HPV clearance in the treated group was significantly higher (81.3%) than in the control group (20%) (p = 0.004). The difference in the regression of the lesion grades almost reached statistical significance (p = 0.053). This follow-up confirms that the micro-immunotherapy drug 2LPAPI<sup><sup>®</sup></sup> is a safe and effective therapeutic approach to treat HR-HPV cervical lesions in women over 25 years.
基金the División de Investigación Universidad Nacional de Colombia,grants No.20201007738 and 202010013254
文摘Bovine Herpesvirus-1 (BoHV-1) is distributed worldwide and is a major pathogen in cattle, being the causal agent of a variety of clinical syndromes. The aim of this study was to isolate and to characterize (molecular and biological characterization) BoHV-1 from 29 immunosuppressed animals. It was possible to obtain 18 isolates, each from a different animal, such as from the respiratory and reproductive tracts. In some cases the cytopathic effect was visible 12 hours post-inoculation, and became characteristic after 36-48 hours. Biological characteristics were evaluated and compared with Iowa and Colorado-1 reference strains, and differences were found in plaque size, virus titer measured by TCID50 and PFU/mL, and one step virus curves. These results showed that some isolates had a highly virulent-like behavior in vitro, compared to the reference strains, with shorter eclipse periods, faster release of virus into the supernatants, and higher burst size and viral titer. There were no differences in glycoprotein expression of BoHV-1 isolates, measured by Western blot on monolayers. Moreover, using restriction endonucleases analysis, most of the viruses were confirmed as BoHV-1.1 and just one of them was confirmed as BoHV-1.2a subtype. These findings suggest that some wild-type BoHV-1 isolates could be useful as seeds to develop new monovalent vaccines.
文摘Aim： To evaluate the association between selected potential socio-demographic and behavioral risk factors and infertility in Nigerian men. Methods： There were two groups in this study. One group consisted of 150 men with proven male infertility, and the other consisted of 150 fertile men with normal semen parameters. Both were matched for age, place of residence and key socio-demographic variables. They were compared for sexual history, past medical and surgical history, past exposures to sexually transmitted infections and treatment, past and current use of drugs as well as smoking and alcohol intake history. Results： Infertile men were significantly more likely than fertile men to report having experienced penile discharge, painful micturition and genital ulcers, less likely to seek treatment for these symptoms and more likely to seek treatment with informal sector providers. Multivariate analysis showed that male infertility was significantly associated with bacteria in semen cultures, self-reporting of previous use of traditional medications and moderate to heavy alcohol intake, but not with smoking and occupational types. Conclusion： Infertility is associated with various proxies of sexually transmitted infections （STIs） and poor healthcare-seeking behavior for STIs in Nigerian men.
文摘Inhibitors of sodium-glucose co-transporter type 2(SGLT2), such as canagliflozin and dapagliflozin, are recently approved for treatment of type 2 diabetes. These agents lower blood glucose mainly by increasing urinary glucose excretion. Compared with placebo, SGLT2 inhibitors reduce hemoglobin A1c(Hb A1c) levels by an average of 0.5%-0.8% when used as monotherapy or add-on therapy. Advantages of this drug class include modest weight loss of approximately 2 kg, low risk of hypoglycemia, and decrease blood pressure of approximately 4 mm Hg systolic and 2 mm Hg diastolic. These characteristics make these agents potential add-on therapy in patients with Hb A1 c levels close to 7%-8.0%, particularly if these patients are obese, hypertensive, and/or prone for hypoglycemia. Meanwhile, these drugs are limited by high frequency of genital mycotic infections. Less common adverse effects include urinary tract infections, hypotension, dizziness, and worsening renal function. SGLT2 inhibitors should be used with caution in the elderly because of increased adverse effects, and should not be used in chronic kidney disease due to decreased or lack of efficacy and nephrotoxicity. Overall, SGLT2 inhibitors are useful addition for treatment of select groups of patients with type 2 diabetes,but their efficacy and safety need to be established in long-term clinical trials.
文摘The aims of this study were （a） to determine the prevalence of subjects with semen hyperviscosity （SHV） in a large population of male partners of subfertile couples; （b） to identify any correlation between SHV and infections or inflammation of the genital tract; （c） to assess the effects of therapeutic approaches for treating SHV; and （d） to assess sperm kinetic parameters after successful treatment of SHV. A retrospective study of 1 833 male partners of subfertile couples was conducted. Next, clinical, seminal, bacteriological and ultrasound studies involving 52 subjects suffering from SHV were performed, and the SHV was classified as being mild （length of thread 〉 2 cm and ≤4 cm）, moderate （〉 4 cm and ≤ 6 cm） or severe （〉 6 cm）. The prevalence of SHV was observed in 26.2% （480） of the subjects, with 13.2% suffering from mild, 6.6% from moderate and 6.4% from severe SHV. Treatment was completely successful in only 27 subjects （52.0%）, primarily in those who had mild basal SHV with a positive semen culture. In these subjects, progressive motility percentage, straight line velocity and linearity were significantly higher than pre-treatment levels. SHV is often found in subjects with subfertility. Pathogenesis was strictly related to infective/inflammatory factors in only 48.0% of cases; therefore, it is possible that biochemical, enzymatic or genetic factors have a role in this condition.
文摘Objective:Genital chlamydial infection is a globally common sexually transmitted infection caused by Chlamydia trachomatis.This study aimed to describe the epidemiological characteristics of the disease reported in China in 2019.Methods:Genital chlamydial infection cases were identified in 105 national sexually transmitted disease surveillance sites from the National Notifiable Infectious Disease Reporting System,which covers 31 provinces,autonomous regions,and municipalities in Chinese mainland.Data were collected and used to calculate the overall incidence,and the incidences per surveillance site,province,sex,and age group.Results:In 2019,there were 50,874 new cases of genital chlamydial infection reported in China,corresponding to an incidence of 55.32 cases per 100,000 population,and comprising a 9.98%increase from 2018(50.30 cases per 100,000 population).The highest incidences of genital chlamydial infection occurred in Zhejiang,Guangdong province,Guangxi autonomous region,and Hainan province(average 137.49-233.37 cases per 100,000 population).The incidence of genital chlamydial infection was much higher in females than males,with a female-to-male ratio of 3.09(84.55 per 100,000 population vs 27.35 per 100,000 population).The highest incidence of genital chlamydial infection was reported in the 20-24-year age group(184.70 cases per 100,000 population),followed by the 25-29-year age group(180.79 cases per 100,000)and 30-34-year age group(151.53 cases per 100,000 population).The highest proportion(68.30%)of genital chlamydial infection was reported from general hospitals,followed by maternal and child health hospitals(25.59%).Conclusion:The incidence rate of genital chlamydial infection in 2019 has an increase trend from 2018 and be a common sexually transmitted infection in China.The incidence is much higher in females than in males.Sexually active young adults are the highest risk population.There is substantial geographic diversity in incidence,with the highest incidence occurring in the southeast coastal areas with a developed economy.Epidemic of genital chlamydial infection in China should be concerned,and its surveillance needs to be improved.
