Objective:To construct a novel nanoplatform GNS@CaCO3/Ce6-NK by loading the CaCO3-coated gold nanostars(GNSs)with Chlorin e6 molecules(Ce6)into human peripheral blood mononuclear cells(PBMCs)-derived NK cells for tumo...Objective:To construct a novel nanoplatform GNS@CaCO3/Ce6-NK by loading the CaCO3-coated gold nanostars(GNSs)with Chlorin e6 molecules(Ce6)into human peripheral blood mononuclear cells(PBMCs)-derived NK cells for tumor targeted therapy.Methods:GNS@CaCO3/Ce6 nanoparticles were prepared and characterized by TEM and UV-vis.The cell surface markers and cytokines secretion of NK cells before and after loading the GNS@CaCO3/Ce6 nanoparticles were detected by Flow Cytometry(FCM)and ELISA.Effects of the GNS@CaCO3/Ce6-NK cells on A549 cancer cells was determined by FCM and CCK-8.Intracellular fluorescent signals of GNS@CaCO3/Ce6-NK cells were detected via Confocal laser scanning microscopic(CLSM)and FCM at different time points.Intracellular ROS generation of GNS@CaCO3/Ce6-NK cells under laser irradiation were examined by FCM.The distribution of GNS@CaCO3/Ce6-NK in A549 tumor-bearing mice were observed by fluorescence imaging and PA imaging.The combination therapy of GNS@CaCO3/Ce6-NK under laser irradiation were investigated on tumor-bearing mice.Results:The coated CaC03 shell on the surface of GNSs exhibited prominent delivery and protection effect of Ce6 during the cellular uptake process.The as-prepared multifunctional GNS@CaCO3/Ce6-NK cells possessed bimodal functions of fluorescence imaging and photoacoustic imaging.The as-prepared multifunctional GNS@CaCO3/Ce6-NK cells could actively target tumor tissues with the enhanced photothermal/photodynamic therapy and immunotherapy.Conclusions:The GNS@CaCO3/Ce6-NK shows effective tumor-targeting ability and prominent therapeutic efficacy toward lung cancer A549 tumor-bearing mice.Through fully utilizing the features of GNSs and NK cells,this new nanoplatform provides a new synergistic strategy for enhanced photothermal/photodynamic therapy and immunotherapy in the field of anticancer development in the near future.展开更多
T-2 toxin,one of the most dangerous natural pollutants,induces apoptosis through multiple pathways.Amongst,P53 mediated apoptosis pathway,an important collection of molecules,plays a key role in cell vital activity.Re...T-2 toxin,one of the most dangerous natural pollutants,induces apoptosis through multiple pathways.Amongst,P53 mediated apoptosis pathway,an important collection of molecules,plays a key role in cell vital activity.Real-time monitoring of upstream and downstream activation relationships of P53 mRNA,Bax mRNA,and cytochrome c(Cyt c)in signaling pathways is of great significance for understanding the apoptotic machinery in human physiology.In this work,a novel nucleic acid multicolor fluorescent probe,based on silica-coated symmetric gold nanostars(S-AuNSs@SiO_(2)),was developed for highly sensitive in situ real-time imaging of P53 mRNA,Bax mRNA,and Cyt c during T-2 toxin-induced apoptosis.The nucleic acid chains modified with carboxyl groups were modified on the surface of S-AuNSs@SiO_(2)by amide reaction.The complementary chains of targeted mRNA and the aptamer of targeted Cyt c were modified with different fluorophores,respectively,and successfully hybridized on S-AuNSs@SiO_(2)surface.When targets were present,the fluorescent chains bound to the targets and detached from the material,resulting in the quenched fluorescence being revived.The probes based on S-AuNSs showed excellent performance is partly ascribed to the presence of 20 symmetric“hot spots”.Notably,the amide-bonded probe exhibited excellent anti-interference capability against biological agents(nucleases and biothiols).During the real-time fluorescence imaging of T-2 toxin-induced apoptosis,the corresponding fluorescence signals of P53 mRNA,Bax mRNA,and Cyt c were observed sequentially.Therefore,S-AuNSs@SiO_(2)probe not only provides a novel tool for real-time monitoring of apoptosis pathways cascade but also has considerable potential in disease diagnosis and pharmaceutical medical.展开更多
In this paper, we propose and demonstrate an all-fiber passively Q-switched erbium doped fiber laser(EDFL) by using gold nanostars(GNSs) as a saturable absorber(SA) for the first time, to the best of our knowledge. In...In this paper, we propose and demonstrate an all-fiber passively Q-switched erbium doped fiber laser(EDFL) by using gold nanostars(GNSs) as a saturable absorber(SA) for the first time, to the best of our knowledge. In comparison with other gold nanomorphologies, GNSs have multiple localized surface plasmon resonances, which means that they can be used to construct wideband ultrafast pulse lasers. By inserting the GNS SA into an EDFL cavity pumped by a 980 nm laser diode, a stable passively Q-switched laser at 1564.5 nm was achieved for a threshold pump power of 40 mW. By gradually increasing the pump power from 40 to 120 mW, the pulse duration decreases from 12.8 to 5.3 μs and the repetition rate increases from 10 to 17 kHz. Our results indicate that the GNSs are a promising SA for constructing pulse lasers.展开更多
Photothermal therapy(PTT)has emerged as a promising cancer therapeutic method.In this study,Arg-Gly-Asp(RGD)peptide-conjugated polydopamine-coated gold nanostars(Au@PDA-RGD NPs)were prepared for targeting PTT of hepat...Photothermal therapy(PTT)has emerged as a promising cancer therapeutic method.In this study,Arg-Gly-Asp(RGD)peptide-conjugated polydopamine-coated gold nanostars(Au@PDA-RGD NPs)were prepared for targeting PTT of hepatocellular carcinoma(HCC).A polydopamine(PDA)shell was coated on the surface of gold nanostars by the oxidative self-polymerization of dopamine(termed as Au@PDA NPs).Au@PDA NPs were further functionalized with polyethylene glycol and RGD peptide to improve biocompatibility as well as selectivity toward the HCC cells.Au@PDARGD NPs showed an intense absorption at 822 nm,which makes them suitable for near-infraredexcited PTT.Our results indicated that the Au@PDA-RGD NPs were effective for the PTT therapy of the α_(v)β_(3) integrin receptor-overexpressed HepG2 cells in vitro.Further antitumor mechanism studies showed that the Au@PDA-RGD NPs-based PTT induced human liver cancer cells death via the mitochondrial-lysosomal and autophagy pathways.In vivo experiments showed that Au@PDARGD NPs had excellent tumor treatment efficiency and negligible side effects.Thus,our study showed that Au@PDA-RGD NPs could offer an excellent nanoplatform for PTT of HCC.展开更多
Gold nanostars(Au NSs)are asymmetric anisotropic nanomaterials with sharp edge structure.As a promising branched nanomaterial,Au NS has excellent plasmonic absorption and scattering properties.In order to tune the pla...Gold nanostars(Au NSs)are asymmetric anisotropic nanomaterials with sharp edge structure.As a promising branched nanomaterial,Au NS has excellent plasmonic absorption and scattering properties.In order to tune the plasmonic photothermal and surface-enhanced Raman scattering(SERS)activity of Au NSs to obtain the desired characteristics,the ffects of reagents on the local surface plasmon resonance(LSPR)bands of Au NSs were studied and the morphology and size were regulated.Nanoparticles with different sharp edges were synthesized to make their local plasmon resonance mode tunable in the visible and near-infrared region.The effects of the number and sharpness of different tips under the control of AgNO3 on the photothermal response of Au NSs and the SERS ac-tivity and their mechanism were discussed in detail.The results show that as the length of the branch tip becomes longer and the sharpness increases,the plasmonic photothermal effect of Au NSs is strengthened,and the photother-mal conversion efficiency is the highest up to 40%when the length of Au NSs is the longest.Au NSs with high SERS activity are used for the Raman detection substrate.Based on this property,the quantitative detection of the pesticide thiram is achieved.展开更多
Lateral flow immunoassay(LFIA) is a widely used express method and offers advantages such as a short analysis time, simplicity of testing and result evaluation.However, an LFIA based on gold nanospheres lacks the desi...Lateral flow immunoassay(LFIA) is a widely used express method and offers advantages such as a short analysis time, simplicity of testing and result evaluation.However, an LFIA based on gold nanospheres lacks the desired sensitivity, thereby limiting its wide applications.In this study, spherical nanogold labels along with new types of nanogold labels such as gold nanopopcorns and nanostars were prepared, characterized, and applied for LFIA of model protein antigen procalcitonin. It was found that the label with a structure close to spherical provided more uniform distribution of specific antibodies on its surface, indicative of its suitability for this type of analysis.LFIA using gold nanopopcorns as a label allowed procalcitonin detection over a linear range of 0.5–10 ng mL^(-1) with the limit of detection of 0.1 ng mL^(-1), which was fivefold higher than the sensitivity of the assay with gold nanospheres. Another approach to improve the sensitivity of the assay included the silver enhancement method,which was used to compare the amplification of LFIA for procalcitonin detection. The sensitivity of procalcitonin determination by this method was 10 times better the sensitivity of the conventional LFIA with gold nanosphere as a label. The proposed approach of LFIA based on gold nanopopcorns improved the detection sensitivity without additional steps and prevented the increased consumption of specific reagents(antibodies).展开更多
Monitoring an infectious disease early using highly sensitive and non-invasive techniques is critical for human health.Interestingly,the development of surfaceenhanced Raman scattering(SERS)for biological detection id...Monitoring an infectious disease early using highly sensitive and non-invasive techniques is critical for human health.Interestingly,the development of surfaceenhanced Raman scattering(SERS)for biological detection ideally fits these medical requirements and is rapidly growing as a powerful diagnostic tool.SERS can enhance the Raman signal of the target molecule by more than 106 after the adsorption of the molecule on the plasmonic nanostructured surface.This review provides an overview of the use of gold and silver nanoparticles in SERS substrate designs,followed by the development of these SERS substrates in severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)detection.展开更多
基金supported from 973 Project (Grant No. 2015CB931802 and 2017YFA0205301)Chinese National Natural Scientific Fund (Grant No.81327002 and 81803094)+1 种基金China Postdoctoral Science Foundation (Grant No. 2017M621486)Funding from Shanghai Engineering Research Center for Intelligent diagnosis and treatment instrument (Grant No.15DZ2252000)
文摘Objective:To construct a novel nanoplatform GNS@CaCO3/Ce6-NK by loading the CaCO3-coated gold nanostars(GNSs)with Chlorin e6 molecules(Ce6)into human peripheral blood mononuclear cells(PBMCs)-derived NK cells for tumor targeted therapy.Methods:GNS@CaCO3/Ce6 nanoparticles were prepared and characterized by TEM and UV-vis.The cell surface markers and cytokines secretion of NK cells before and after loading the GNS@CaCO3/Ce6 nanoparticles were detected by Flow Cytometry(FCM)and ELISA.Effects of the GNS@CaCO3/Ce6-NK cells on A549 cancer cells was determined by FCM and CCK-8.Intracellular fluorescent signals of GNS@CaCO3/Ce6-NK cells were detected via Confocal laser scanning microscopic(CLSM)and FCM at different time points.Intracellular ROS generation of GNS@CaCO3/Ce6-NK cells under laser irradiation were examined by FCM.The distribution of GNS@CaCO3/Ce6-NK in A549 tumor-bearing mice were observed by fluorescence imaging and PA imaging.The combination therapy of GNS@CaCO3/Ce6-NK under laser irradiation were investigated on tumor-bearing mice.Results:The coated CaC03 shell on the surface of GNSs exhibited prominent delivery and protection effect of Ce6 during the cellular uptake process.The as-prepared multifunctional GNS@CaCO3/Ce6-NK cells possessed bimodal functions of fluorescence imaging and photoacoustic imaging.The as-prepared multifunctional GNS@CaCO3/Ce6-NK cells could actively target tumor tissues with the enhanced photothermal/photodynamic therapy and immunotherapy.Conclusions:The GNS@CaCO3/Ce6-NK shows effective tumor-targeting ability and prominent therapeutic efficacy toward lung cancer A549 tumor-bearing mice.Through fully utilizing the features of GNSs and NK cells,this new nanoplatform provides a new synergistic strategy for enhanced photothermal/photodynamic therapy and immunotherapy in the field of anticancer development in the near future.
基金the financial support from the Jiangsu Agriculture Science and Technology Innovation Fund(No.CX(19)2005)the Social Development Fund Project of Wuxi(No.N20201001).
文摘T-2 toxin,one of the most dangerous natural pollutants,induces apoptosis through multiple pathways.Amongst,P53 mediated apoptosis pathway,an important collection of molecules,plays a key role in cell vital activity.Real-time monitoring of upstream and downstream activation relationships of P53 mRNA,Bax mRNA,and cytochrome c(Cyt c)in signaling pathways is of great significance for understanding the apoptotic machinery in human physiology.In this work,a novel nucleic acid multicolor fluorescent probe,based on silica-coated symmetric gold nanostars(S-AuNSs@SiO_(2)),was developed for highly sensitive in situ real-time imaging of P53 mRNA,Bax mRNA,and Cyt c during T-2 toxin-induced apoptosis.The nucleic acid chains modified with carboxyl groups were modified on the surface of S-AuNSs@SiO_(2)by amide reaction.The complementary chains of targeted mRNA and the aptamer of targeted Cyt c were modified with different fluorophores,respectively,and successfully hybridized on S-AuNSs@SiO_(2)surface.When targets were present,the fluorescent chains bound to the targets and detached from the material,resulting in the quenched fluorescence being revived.The probes based on S-AuNSs showed excellent performance is partly ascribed to the presence of 20 symmetric“hot spots”.Notably,the amide-bonded probe exhibited excellent anti-interference capability against biological agents(nucleases and biothiols).During the real-time fluorescence imaging of T-2 toxin-induced apoptosis,the corresponding fluorescence signals of P53 mRNA,Bax mRNA,and Cyt c were observed sequentially.Therefore,S-AuNSs@SiO_(2)probe not only provides a novel tool for real-time monitoring of apoptosis pathways cascade but also has considerable potential in disease diagnosis and pharmaceutical medical.
基金National Natural Science Foundation of China(NSFC)(11474132,61378004,61527823,61605058,61605219)Natural Science Foundation of Jilin Province(20160520085JH)+3 种基金Key Technology Research and Development Project of Jilin Province(20180201120GX)Major Science and Technology Tendering Project of Jilin Province(20170203012GX)Joint Foundation from Equipment Pre-research and Ministry of Education(6141A02022413)Outstanding Young Talent Fund Project of Jilin Province(20180520188JH)
文摘In this paper, we propose and demonstrate an all-fiber passively Q-switched erbium doped fiber laser(EDFL) by using gold nanostars(GNSs) as a saturable absorber(SA) for the first time, to the best of our knowledge. In comparison with other gold nanomorphologies, GNSs have multiple localized surface plasmon resonances, which means that they can be used to construct wideband ultrafast pulse lasers. By inserting the GNS SA into an EDFL cavity pumped by a 980 nm laser diode, a stable passively Q-switched laser at 1564.5 nm was achieved for a threshold pump power of 40 mW. By gradually increasing the pump power from 40 to 120 mW, the pulse duration decreases from 12.8 to 5.3 μs and the repetition rate increases from 10 to 17 kHz. Our results indicate that the GNSs are a promising SA for constructing pulse lasers.
基金support from National Natural Science Foundation of China(NSFC81602736)China Postdoctoral Science Foundation(2018M632684)+1 种基金Shandong Provincial Natural Science Foundation(ZR2019PH084)Liaocheng People’s Hospital Youth Research Fund(LYQN201935).
文摘Photothermal therapy(PTT)has emerged as a promising cancer therapeutic method.In this study,Arg-Gly-Asp(RGD)peptide-conjugated polydopamine-coated gold nanostars(Au@PDA-RGD NPs)were prepared for targeting PTT of hepatocellular carcinoma(HCC).A polydopamine(PDA)shell was coated on the surface of gold nanostars by the oxidative self-polymerization of dopamine(termed as Au@PDA NPs).Au@PDA NPs were further functionalized with polyethylene glycol and RGD peptide to improve biocompatibility as well as selectivity toward the HCC cells.Au@PDARGD NPs showed an intense absorption at 822 nm,which makes them suitable for near-infraredexcited PTT.Our results indicated that the Au@PDA-RGD NPs were effective for the PTT therapy of the α_(v)β_(3) integrin receptor-overexpressed HepG2 cells in vitro.Further antitumor mechanism studies showed that the Au@PDA-RGD NPs-based PTT induced human liver cancer cells death via the mitochondrial-lysosomal and autophagy pathways.In vivo experiments showed that Au@PDARGD NPs had excellent tumor treatment efficiency and negligible side effects.Thus,our study showed that Au@PDA-RGD NPs could offer an excellent nanoplatform for PTT of HCC.
基金Supported by the National Natural Science Foundation of China(No.11774048)the"111"Project of China(No.B13013).
文摘Gold nanostars(Au NSs)are asymmetric anisotropic nanomaterials with sharp edge structure.As a promising branched nanomaterial,Au NS has excellent plasmonic absorption and scattering properties.In order to tune the plasmonic photothermal and surface-enhanced Raman scattering(SERS)activity of Au NSs to obtain the desired characteristics,the ffects of reagents on the local surface plasmon resonance(LSPR)bands of Au NSs were studied and the morphology and size were regulated.Nanoparticles with different sharp edges were synthesized to make their local plasmon resonance mode tunable in the visible and near-infrared region.The effects of the number and sharpness of different tips under the control of AgNO3 on the photothermal response of Au NSs and the SERS ac-tivity and their mechanism were discussed in detail.The results show that as the length of the branch tip becomes longer and the sharpness increases,the plasmonic photothermal effect of Au NSs is strengthened,and the photother-mal conversion efficiency is the highest up to 40%when the length of Au NSs is the longest.Au NSs with high SERS activity are used for the Raman detection substrate.Based on this property,the quantitative detection of the pesticide thiram is achieved.
基金financially supported by the Ministry of Education and Science of the Russian Federation in the framework of increase Competitiveness Program of NUST ‘‘MISIS’’, implemented by a governmental decree dated 16th of March 2013, No. 211part of state assignment Organization of scientific researches (Project No. 16.6548.2017/BY)
文摘Lateral flow immunoassay(LFIA) is a widely used express method and offers advantages such as a short analysis time, simplicity of testing and result evaluation.However, an LFIA based on gold nanospheres lacks the desired sensitivity, thereby limiting its wide applications.In this study, spherical nanogold labels along with new types of nanogold labels such as gold nanopopcorns and nanostars were prepared, characterized, and applied for LFIA of model protein antigen procalcitonin. It was found that the label with a structure close to spherical provided more uniform distribution of specific antibodies on its surface, indicative of its suitability for this type of analysis.LFIA using gold nanopopcorns as a label allowed procalcitonin detection over a linear range of 0.5–10 ng mL^(-1) with the limit of detection of 0.1 ng mL^(-1), which was fivefold higher than the sensitivity of the assay with gold nanospheres. Another approach to improve the sensitivity of the assay included the silver enhancement method,which was used to compare the amplification of LFIA for procalcitonin detection. The sensitivity of procalcitonin determination by this method was 10 times better the sensitivity of the conventional LFIA with gold nanosphere as a label. The proposed approach of LFIA based on gold nanopopcorns improved the detection sensitivity without additional steps and prevented the increased consumption of specific reagents(antibodies).
基金Australian Research Council(ARC),Grant/Award Number:DP200101217。
文摘Monitoring an infectious disease early using highly sensitive and non-invasive techniques is critical for human health.Interestingly,the development of surfaceenhanced Raman scattering(SERS)for biological detection ideally fits these medical requirements and is rapidly growing as a powerful diagnostic tool.SERS can enhance the Raman signal of the target molecule by more than 106 after the adsorption of the molecule on the plasmonic nanostructured surface.This review provides an overview of the use of gold and silver nanoparticles in SERS substrate designs,followed by the development of these SERS substrates in severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)detection.
基金supported by NTU-Northwestern Institute for Nanomedicine(To Xu CJ)the Tier-2 Grant funded by the Ministry of Education in Singapore(ARC2/15:M4020238 to M.R)
