Objective:To investigate the effect of humic acid on nematode infected,resistant and susceptible grapes in relation to lipid peroxidation and antioxidant mechanisms on selected biochemical parameters known as proactiv...Objective:To investigate the effect of humic acid on nematode infected,resistant and susceptible grapes in relation to lipid peroxidation and antioxidant mechanisms on selected biochemical parameters known as proactive substances.Methods:The grape rootstocks,superior,superior/ freedom and freedom were reacted differently to Meloidogyne incognita and Rotylenchulus reniformis according to rootstock progenitor.Two weeks after inoculation,two commercial products of humic acid were applied at the rate of(2,4 mL or grams/plant) as soil drench.After 4 months,nematode soil populations were extracted and counted.A subsample of roots from each plant was stained and gall numbers,embedded stages per root were calculated,final population, nematode build up(Pf/Pi),average of eggs/eggmass were estimated.Subsamples of fresh root of each treatment were chemically analyzed.Results:Freedom reduced significantly the nematode criteria and build up.Humic acid granules appeared to be more suppressive to nematode build up on superior and the higher dose on superior/freedom than liquid treatments.On freedom,all treatments reduced significantly the nematode build up regardless to the material nature.The higher dose was more effective than the lower one.As a result of humic acid applications,the malondialdehyde(MDA) and H_2O_2 contents were significandy reduced after humic acid treatments while the antioxidant compounds glutathione(GSH),ascorbic acid(ASA) and total phenol contents were significandy increased when compared with check.Antioxidant defense enzymes ascorbate peroxidase(AFX),superoxide dismutase(SOD),catalase(CAT) and polyphenol oxidase(PPO) showed significant increase in their specific activities in treated plants compared with nematode treated check.Conclnsions:Humic acid treatments improve we yield of grape by increasing the contents of antioxidant compounds and the specific activities of antioxidant enzymes.展开更多
[目的]气候变化新形势下,进一步筛选适宜西北地区抗寒、抗抽干葡萄砧木品种,可为葡萄免埋土栽培推广提供理论依据和技术支撑。[方法]本文以41Bmgt、Riparia、420Bmgt、101-14、196-17、44-53ma、110R、Rupestris du Lot、SO4、161-490、...[目的]气候变化新形势下,进一步筛选适宜西北地区抗寒、抗抽干葡萄砧木品种,可为葡萄免埋土栽培推广提供理论依据和技术支撑。[方法]本文以41Bmgt、Riparia、420Bmgt、101-14、196-17、44-53ma、110R、Rupestris du Lot、SO4、161-490、1103P、5BB、3309、Leon Millt共14种13年生葡萄砧木为试材,通过测定离体一年生枝条累计失水速率,结合田间调查葡萄砧木存活率和离体一年生枝条抽干率,以此来评价不同葡萄砧木品种抗抽干能力;结合前人研究及砧木品种特性,采用高低温交变试验箱模拟低温的方法,设置4(对照)、-15、-20、-25和-30℃一系列温度梯度水平,测定不同葡萄砧木一年生枝条相对电导率、丙二醛、游离脯氨酸、可溶性糖及可溶性蛋白5个生理生化指标,利用隶属函数法进行综合评价不同葡萄砧木的抗寒性。[结果]不同葡萄砧木的抗抽干能力由强到弱依次为:3309>161-490>196-17>Leon Millt>420Bmgt>44-53ma>Riparia>110R>1103P>SO4>101-14>41Bmgt>5BB>Rupestris du Lot;随着处理温度的降低,不同葡萄砧木枝条的抗寒指标总体呈现上升趋势,但不同品种之间存在差异。根据隶属函数的综合分析,结果显示各葡萄砧木的抗寒性由强到弱依次为:SO4>196-17>Leon Millt>5BB>Riparia>44-53ma>420Bmgt>1103P>3309>110R>Rupestris du Lot>101-14>41Bmgt>161-490。[结论]Riparia、196-17、44-53ma和Leon Millt具有较强的抗抽干能力和抗寒性,可作为我国西北地区免埋土葡萄砧木使用。展开更多
基金financially supported by Management of the Faculty of Agriculture and Cario Univerisity
文摘Objective:To investigate the effect of humic acid on nematode infected,resistant and susceptible grapes in relation to lipid peroxidation and antioxidant mechanisms on selected biochemical parameters known as proactive substances.Methods:The grape rootstocks,superior,superior/ freedom and freedom were reacted differently to Meloidogyne incognita and Rotylenchulus reniformis according to rootstock progenitor.Two weeks after inoculation,two commercial products of humic acid were applied at the rate of(2,4 mL or grams/plant) as soil drench.After 4 months,nematode soil populations were extracted and counted.A subsample of roots from each plant was stained and gall numbers,embedded stages per root were calculated,final population, nematode build up(Pf/Pi),average of eggs/eggmass were estimated.Subsamples of fresh root of each treatment were chemically analyzed.Results:Freedom reduced significantly the nematode criteria and build up.Humic acid granules appeared to be more suppressive to nematode build up on superior and the higher dose on superior/freedom than liquid treatments.On freedom,all treatments reduced significantly the nematode build up regardless to the material nature.The higher dose was more effective than the lower one.As a result of humic acid applications,the malondialdehyde(MDA) and H_2O_2 contents were significandy reduced after humic acid treatments while the antioxidant compounds glutathione(GSH),ascorbic acid(ASA) and total phenol contents were significandy increased when compared with check.Antioxidant defense enzymes ascorbate peroxidase(AFX),superoxide dismutase(SOD),catalase(CAT) and polyphenol oxidase(PPO) showed significant increase in their specific activities in treated plants compared with nematode treated check.Conclnsions:Humic acid treatments improve we yield of grape by increasing the contents of antioxidant compounds and the specific activities of antioxidant enzymes.
文摘[目的]气候变化新形势下,进一步筛选适宜西北地区抗寒、抗抽干葡萄砧木品种,可为葡萄免埋土栽培推广提供理论依据和技术支撑。[方法]本文以41Bmgt、Riparia、420Bmgt、101-14、196-17、44-53ma、110R、Rupestris du Lot、SO4、161-490、1103P、5BB、3309、Leon Millt共14种13年生葡萄砧木为试材,通过测定离体一年生枝条累计失水速率,结合田间调查葡萄砧木存活率和离体一年生枝条抽干率,以此来评价不同葡萄砧木品种抗抽干能力;结合前人研究及砧木品种特性,采用高低温交变试验箱模拟低温的方法,设置4(对照)、-15、-20、-25和-30℃一系列温度梯度水平,测定不同葡萄砧木一年生枝条相对电导率、丙二醛、游离脯氨酸、可溶性糖及可溶性蛋白5个生理生化指标,利用隶属函数法进行综合评价不同葡萄砧木的抗寒性。[结果]不同葡萄砧木的抗抽干能力由强到弱依次为:3309>161-490>196-17>Leon Millt>420Bmgt>44-53ma>Riparia>110R>1103P>SO4>101-14>41Bmgt>5BB>Rupestris du Lot;随着处理温度的降低,不同葡萄砧木枝条的抗寒指标总体呈现上升趋势,但不同品种之间存在差异。根据隶属函数的综合分析,结果显示各葡萄砧木的抗寒性由强到弱依次为:SO4>196-17>Leon Millt>5BB>Riparia>44-53ma>420Bmgt>1103P>3309>110R>Rupestris du Lot>101-14>41Bmgt>161-490。[结论]Riparia、196-17、44-53ma和Leon Millt具有较强的抗抽干能力和抗寒性,可作为我国西北地区免埋土葡萄砧木使用。
