Underweight and specific nutrient deficiencies are frequent in adult patients with inflammatory bowel disease(IBD).In addition,a significant number of children with IBD,especially Crohn's disease(CD) have impaired...Underweight and specific nutrient deficiencies are frequent in adult patients with inflammatory bowel disease(IBD).In addition,a significant number of children with IBD,especially Crohn's disease(CD) have impaired linear growth.Nutrition has an important role in the management of IBD.In adults with CD,enteral nutrition(EN) is effective in inducing clinical remission of IBD,although it is less efficient than corticosteroids.Exclusive EN is an established primary therapy for pediatric CD.Limited data suggests that EN is as efficient as corticosteroids for induction of remission.Additional advantages of nutritional therapy are control of inflammation,mucosal healing,positive benefits to growth and overall nutritional status with minimal adverse effects.The available evidence suggests that supplementary EN may be effective also for maintenance of remission in CD.More studies are needed to confirm these findings.However,EN supplementation could be considered as an alternative or as an adjunct to maintenance drug therapy in CD.EN does not have a primary therapeutic role in ulcerative colitis.Specific compositions of enteral dietselemental diets or diets containing specific components-were not shown to have any advantage over standard polymeric diets and their place in the treatment of CD or UC need further evaluation.Recent theories suggest that diet may be implicated in the etiology of IBD,however there are no proven dietary approaches to reduce the risk of developing IBD.展开更多
AIM： To determine the prevalence of celiac disease （CD） in children with idiopathic short stature （ISS） and the diagnostic value of immunoglobulin （Ig） A G antigliadin antibodies （AGA） and transglutaminase （...AIM： To determine the prevalence of celiac disease （CD） in children with idiopathic short stature （ISS） and the diagnostic value of immunoglobulin （Ig） A G antigliadin antibodies （AGA） and transglutaminase （TTG） antibodies for CD.METHODS： A total of 104 children （49 male, 55 female） with ISS without a specific etiology were studied. Extensive endocrine investigations had shown no abnormalities in any subject. Anthropometric parameters and IgA AGA and IgA TTG antibodies were evaluated in this study group. These antibodies were measured by enzyme-linked immunosorbent assay. All patients were referred for an endoscopic intestinal biopsy. The biopsy samples were classified according to revised Marsh criteria （UEGW 2001）.RESULTS： We detected positive IgA TTG antibodies in 36 and IgA AGA in 35 of these patients. Thirty one IgA TTG antibody positive and 28 IgA AGA positive subjects showed histological abnormalities compatible with celiac disease （33.6%）. Sensitivity, specificity, positive predictive value （PPV） and negative predictive value for IgA AGA were found to be 80%, 88.4%, 77.8% and 89.7%, respectively. Sensitivity, specificity and PPV for IgA TTG antibodies were 88.6%, 94.2% and 88.6%, espectively.CONCLUSION： We conclude that the prevalence of celiac disease is high in patients with ISS and it is important to test all children with ISS for celiac disease by measuring serologic markers and performing an intestinal biopsy.展开更多
BACKGROUND： Zinc （Zn） has been shown to greatly influence brain development. Zn supplements may reduce injury to cell membranes of the thyroid gland due to iodine deficiency. OBJECTIVE： To establish an iodine defi...BACKGROUND： Zinc （Zn） has been shown to greatly influence brain development. Zn supplements may reduce injury to cell membranes of the thyroid gland due to iodine deficiency. OBJECTIVE： To establish an iodine deficiency rat model using low-iodine food, which was supplemented with compound Zn and Zn gluconate, to observe the effects of Zn on brain development, as well as pituitary gland and thyroid gland function in iodine-deficient rats. DESIGN, TIME AND SETTING： Randomized grouping study of neural development was performed in the central laboratory of Shandong Institute for Prevention and Treatment of Endemic Disease from 1998 to 1999. MATERIALS： A total of 270 Wistar, female rats, one month after weaning, were used in this study, including 150 pregnant and 120 neonatal rats. Rats were randomly divided into six groups： normal control, model, iodine, compound Zn, iodine and compound Zn, and zinc gluconate. Each group contained 25 pregnant rats and 20 neonatal rats. METHODS： The pregnant rats and 20 neonatal rats, and well as the normal group, were fed standard chow and allowed free access to tap water （containing 5 u g/L iodine and 1 mg/L Zn）. The remaining five groups were fed low-iodine chow. However, the model group received distilled water, the iodine group received potassium-iodide distilled water （containing 300 u g/L iodine）, the compound Zn group received distilled water and intragastrically administrated 10 mL/kg compound Zn solution, once per day, the iodine and compound Zn group received distilled water with 300 u g/L iodine and intragastrically administrated 10 mL/kg compound Zn solution, once per day. All treatments lasted 90 days. MAIN OUTCOME MEASURES： All pregnant rats were sacrificed on the day 21 of pregnancy. Body mass number and rate of fetal absorption, as well as fetal death and malformation, were determined. Thyroid and pituitary gland weights were measured, as well as serum levels of thyroid hormone, gonadotropin, and sex hormones. In the experimental study of neonatal rats, the animals normally gave birth at day 21. At day 45 after birth of the neonatal rats, thyroid and pituitary gland weights were measured, and protein, DNA, and RNA concentrations were measured. RESULTS： Pregnant rats in the iodine group exhibited decreased urine iodine and body mass （F = 7.37, P 〈 0.01 ）, increased thyroid absolute and relative weight （F= 7.01, 50.27, P 〈 0.01）, as well as decreased T4 and FF4 （F = 7.01, 29.32, P 〈 0.01 ） and increased T3 and VI＇3 （F = 41.20, 5.94, P 〈 0.01）. Gonadotropic and sexual hormones were abnormal. The pregnant rats displayed decreased weight gain, and the rates of malformation, dead, and absorbed fetuses were increased. Compared with the control group, the neonatal rats with iodine deficiency exhibited lower brain weights （P 〈 0.01 ）. Brain protein, DNA, and RNA, concentrations were decreased, with a rate of RNA/DNA （F = 5.70, 55.86, 25.65, 5.44, P 〈 0.01）. Body mass was gradually increased （F= 6.74, P 〈 0.01）, and the thyroid glands were enlarged （F= 50.01, 76.13, P 〈 0.01）. Following Zn administration, thyroid gland weight was decreased in pregnant rats （P 〈 0.01 ）. Thyroid hormone, gonadotropic hormones, and sexual hormones were restored to some degree. Fetal weight was increased, and the rates of malformation, dead, and absorbed fetuses were decreased. At the same time, neonatal rats gained body weight, displayed decreased thyroid gland weight, as well as increased protein, DNA, and RNA concentrations in the brain. The ratio of RNA/DNA and protein/DNA increased following Zn administration （P 〈 0.01 ）. CONCLUSION： Zn supplementation may decrease the degree of goiter, ameliorate thyroid hormone disorder, as well as gonadotropic and sexual hormone disorders, and increase protein, DNA, and RNA content. Zn supplementation antagonized reproductive abnormalities in pregnant rats, decreased fetal growth,and disturbed brain development in neonatal rats as a result of iodine deficiency.展开更多
Objective: To explore the inhibitory effect of He Ne laser irradiation on fibroblast growth of hypertrophic scars in culture. Methods: He Ne laser with wavelength of 632.8 nm, power density of 50 mW/cm 2 and doses of ...Objective: To explore the inhibitory effect of He Ne laser irradiation on fibroblast growth of hypertrophic scars in culture. Methods: He Ne laser with wavelength of 632.8 nm, power density of 50 mW/cm 2 and doses of 3 J/cm 2, 30 J/cm 2 , 90 J/cm 2 and 180 J/cm 2 was used to irradiate human scar fibroblasts in culture 1, 3 and 5 times respectively, and then the cell count and cell cycle analysis were done. Results: Repeated irradiation with He Ne laser at dose of 180 J/cm 2 three and five times led to an evident decrease in total cell number compared with that of the control group and there was a significant difference (P< 0.05 ). The cell cycle analysis showed after three and five times of irradiation with 180 J/cm 2 He Ne laser the cell number in S phase decreased from 51% to 20% and 14% respectively, the cell number in G 0/G 1 phase increased from 28% to 55% and 60% respectively, and the cell percentage in Sub G1 phase was 6.7 % and 9.8 % respectively.Conclusions: Repeated irradiation with 180 J/cm 2 He Ne laser can inhibit scar fibroblasts growth in culture. It may be that He Ne laser irradiation causes cell stagnation in G 0/G 1 phase and apoptosis.展开更多
文摘Underweight and specific nutrient deficiencies are frequent in adult patients with inflammatory bowel disease(IBD).In addition,a significant number of children with IBD,especially Crohn's disease(CD) have impaired linear growth.Nutrition has an important role in the management of IBD.In adults with CD,enteral nutrition(EN) is effective in inducing clinical remission of IBD,although it is less efficient than corticosteroids.Exclusive EN is an established primary therapy for pediatric CD.Limited data suggests that EN is as efficient as corticosteroids for induction of remission.Additional advantages of nutritional therapy are control of inflammation,mucosal healing,positive benefits to growth and overall nutritional status with minimal adverse effects.The available evidence suggests that supplementary EN may be effective also for maintenance of remission in CD.More studies are needed to confirm these findings.However,EN supplementation could be considered as an alternative or as an adjunct to maintenance drug therapy in CD.EN does not have a primary therapeutic role in ulcerative colitis.Specific compositions of enteral dietselemental diets or diets containing specific components-were not shown to have any advantage over standard polymeric diets and their place in the treatment of CD or UC need further evaluation.Recent theories suggest that diet may be implicated in the etiology of IBD,however there are no proven dietary approaches to reduce the risk of developing IBD.
基金Supported by A Grant from the Ahwaz Jondishapoor University of Medical Science, Ahwaz, Iran
文摘AIM： To determine the prevalence of celiac disease （CD） in children with idiopathic short stature （ISS） and the diagnostic value of immunoglobulin （Ig） A G antigliadin antibodies （AGA） and transglutaminase （TTG） antibodies for CD.METHODS： A total of 104 children （49 male, 55 female） with ISS without a specific etiology were studied. Extensive endocrine investigations had shown no abnormalities in any subject. Anthropometric parameters and IgA AGA and IgA TTG antibodies were evaluated in this study group. These antibodies were measured by enzyme-linked immunosorbent assay. All patients were referred for an endoscopic intestinal biopsy. The biopsy samples were classified according to revised Marsh criteria （UEGW 2001）.RESULTS： We detected positive IgA TTG antibodies in 36 and IgA AGA in 35 of these patients. Thirty one IgA TTG antibody positive and 28 IgA AGA positive subjects showed histological abnormalities compatible with celiac disease （33.6%）. Sensitivity, specificity, positive predictive value （PPV） and negative predictive value for IgA AGA were found to be 80%, 88.4%, 77.8% and 89.7%, respectively. Sensitivity, specificity and PPV for IgA TTG antibodies were 88.6%, 94.2% and 88.6%, espectively.CONCLUSION： We conclude that the prevalence of celiac disease is high in patients with ISS and it is important to test all children with ISS for celiac disease by measuring serologic markers and performing an intestinal biopsy.
基金the Scientific and Technology Bureau of Shandong Province, No. 1996 BBIDKA1
文摘BACKGROUND： Zinc （Zn） has been shown to greatly influence brain development. Zn supplements may reduce injury to cell membranes of the thyroid gland due to iodine deficiency. OBJECTIVE： To establish an iodine deficiency rat model using low-iodine food, which was supplemented with compound Zn and Zn gluconate, to observe the effects of Zn on brain development, as well as pituitary gland and thyroid gland function in iodine-deficient rats. DESIGN, TIME AND SETTING： Randomized grouping study of neural development was performed in the central laboratory of Shandong Institute for Prevention and Treatment of Endemic Disease from 1998 to 1999. MATERIALS： A total of 270 Wistar, female rats, one month after weaning, were used in this study, including 150 pregnant and 120 neonatal rats. Rats were randomly divided into six groups： normal control, model, iodine, compound Zn, iodine and compound Zn, and zinc gluconate. Each group contained 25 pregnant rats and 20 neonatal rats. METHODS： The pregnant rats and 20 neonatal rats, and well as the normal group, were fed standard chow and allowed free access to tap water （containing 5 u g/L iodine and 1 mg/L Zn）. The remaining five groups were fed low-iodine chow. However, the model group received distilled water, the iodine group received potassium-iodide distilled water （containing 300 u g/L iodine）, the compound Zn group received distilled water and intragastrically administrated 10 mL/kg compound Zn solution, once per day, the iodine and compound Zn group received distilled water with 300 u g/L iodine and intragastrically administrated 10 mL/kg compound Zn solution, once per day. All treatments lasted 90 days. MAIN OUTCOME MEASURES： All pregnant rats were sacrificed on the day 21 of pregnancy. Body mass number and rate of fetal absorption, as well as fetal death and malformation, were determined. Thyroid and pituitary gland weights were measured, as well as serum levels of thyroid hormone, gonadotropin, and sex hormones. In the experimental study of neonatal rats, the animals normally gave birth at day 21. At day 45 after birth of the neonatal rats, thyroid and pituitary gland weights were measured, and protein, DNA, and RNA concentrations were measured. RESULTS： Pregnant rats in the iodine group exhibited decreased urine iodine and body mass （F = 7.37, P 〈 0.01 ）, increased thyroid absolute and relative weight （F= 7.01, 50.27, P 〈 0.01）, as well as decreased T4 and FF4 （F = 7.01, 29.32, P 〈 0.01 ） and increased T3 and VI＇3 （F = 41.20, 5.94, P 〈 0.01）. Gonadotropic and sexual hormones were abnormal. The pregnant rats displayed decreased weight gain, and the rates of malformation, dead, and absorbed fetuses were increased. Compared with the control group, the neonatal rats with iodine deficiency exhibited lower brain weights （P 〈 0.01 ）. Brain protein, DNA, and RNA, concentrations were decreased, with a rate of RNA/DNA （F = 5.70, 55.86, 25.65, 5.44, P 〈 0.01）. Body mass was gradually increased （F= 6.74, P 〈 0.01）, and the thyroid glands were enlarged （F= 50.01, 76.13, P 〈 0.01）. Following Zn administration, thyroid gland weight was decreased in pregnant rats （P 〈 0.01 ）. Thyroid hormone, gonadotropic hormones, and sexual hormones were restored to some degree. Fetal weight was increased, and the rates of malformation, dead, and absorbed fetuses were decreased. At the same time, neonatal rats gained body weight, displayed decreased thyroid gland weight, as well as increased protein, DNA, and RNA concentrations in the brain. The ratio of RNA/DNA and protein/DNA increased following Zn administration （P 〈 0.01 ）. CONCLUSION： Zn supplementation may decrease the degree of goiter, ameliorate thyroid hormone disorder, as well as gonadotropic and sexual hormone disorders, and increase protein, DNA, and RNA content. Zn supplementation antagonized reproductive abnormalities in pregnant rats, decreased fetal growth,and disturbed brain development in neonatal rats as a result of iodine deficiency.
文摘Objective: To explore the inhibitory effect of He Ne laser irradiation on fibroblast growth of hypertrophic scars in culture. Methods: He Ne laser with wavelength of 632.8 nm, power density of 50 mW/cm 2 and doses of 3 J/cm 2, 30 J/cm 2 , 90 J/cm 2 and 180 J/cm 2 was used to irradiate human scar fibroblasts in culture 1, 3 and 5 times respectively, and then the cell count and cell cycle analysis were done. Results: Repeated irradiation with He Ne laser at dose of 180 J/cm 2 three and five times led to an evident decrease in total cell number compared with that of the control group and there was a significant difference (P< 0.05 ). The cell cycle analysis showed after three and five times of irradiation with 180 J/cm 2 He Ne laser the cell number in S phase decreased from 51% to 20% and 14% respectively, the cell number in G 0/G 1 phase increased from 28% to 55% and 60% respectively, and the cell percentage in Sub G1 phase was 6.7 % and 9.8 % respectively.Conclusions: Repeated irradiation with 180 J/cm 2 He Ne laser can inhibit scar fibroblasts growth in culture. It may be that He Ne laser irradiation causes cell stagnation in G 0/G 1 phase and apoptosis.
