According to the measured groundwater pollution concentration at two monitoring sections of Gusong River coast,adopting progressive dislocation correlation analysis method,correlation between surface water of Gusong R...According to the measured groundwater pollution concentration at two monitoring sections of Gusong River coast,adopting progressive dislocation correlation analysis method,correlation between surface water of Gusong River and groundwater pollution was analyzed.Result showed that propagation time of pollutant between river water and groundwater was longer.According to the water level observation data,river water level was＆gt;1 m higher than groundwater table.The groundwater infiltration parameter was determined through the field permeability test data.The Dupuit formula was used to calculate recharge and discharge relationship between river surface water and groundwater.It was obtained that the daily average recharge capacity of right-bank river water to groundwater was 3 m3,and pollution of river surface water had very small influence on groundwater.Using MATLAB software for data analysis and processing,regression equation was established.The chloride as correlation analysis example,results showed that groundwater chloride had the maximum correlation with observation well distance,and correlation with time and monitoring value of surface water was very small.The linear relationship between surface water and groundwater was not obvious.展开更多
Objective: To assess the effect of Gusong II in treating postmenopausal osteoporosis and try to reveal its mechanism. Methods: Thirty-five women, 49 to 62 years old, with 5. 6 years menopause, were treated as the subj...Objective: To assess the effect of Gusong II in treating postmenopausal osteoporosis and try to reveal its mechanism. Methods: Thirty-five women, 49 to 62 years old, with 5. 6 years menopause, were treated as the subjects and followed-up for 6 months, 12 months or 24 months. The course of treatment ranged from6 months to 25 months with an average of 14 months. Some related biochemical parameters, such as serumestradiol (E2 ), testosterone (T), the ratio of fasting urinary calcium to creatinine (Ca/Cr), the ratio of 24hurinary calcium to creatinine (24h Ca/Cr), 24h urinary E2, and 24h urinary hydroxyproline (HOP) were measured before and after treatment. Results: After 6～9 months or 1～2 years of treatment, the levels of serum E2and 24h urinary E2 were raised significantly, while the levels of serum T and urinary Ca/Cr fell evidently afterone or two year's treatment. The levels of serum E2 and 24h urinary E2 were significantly correlated with thelevels of fasting urinary Ca/Cr, 24h urinary Ca/Cr, serum T, and 24h urinary HOP respectively. Conclusions:Gusong-II can elevate the serum E2 levels of postmenopausal women, reduce the discharge of urinary calcium andHOP, and inhibit bone resorption. The rise of the serum EZ level might be correlated with the conversion of T toE2.展开更多
基于内质网应激(ERS)诱导的细胞凋亡探究骨松强骨方(GSQG)减轻去卵巢小鼠骨丢失的作用与机制。小鼠卵巢摘除(OVX)骨质疏松造模完成后,根据随机数字法,将60只小鼠随机分为6组:假手术组,模型组,骨松强骨方低(GSQG-L)、中(GSQG-M)、高剂量(...基于内质网应激(ERS)诱导的细胞凋亡探究骨松强骨方(GSQG)减轻去卵巢小鼠骨丢失的作用与机制。小鼠卵巢摘除(OVX)骨质疏松造模完成后,根据随机数字法,将60只小鼠随机分为6组:假手术组,模型组,骨松强骨方低(GSQG-L)、中(GSQG-M)、高剂量(GSQG-H)组,雌二醇(E2)组,每组10只;切除双侧卵巢构建模型。术后1个月开始灌胃给药,连续给药3个月。酶联免疫吸附测定法(ELISA)测定血清中骨形成指标骨钙素(OCN)、Ⅰ型胶原氨基端前肽(PINP)和骨吸收指标Ⅰ型胶原交联羧基端肽(CTX)、抗酒石酸酸性磷酸酶5b(TRAcP-5b)的水平,使用Micro-CT观察股骨远端骨微结构的改变,HE染色观察骨组织形态,RT-qPCR检测胫骨干成骨相关基因Ⅰ型胶原(Col-Ⅰ)、碱性磷酸酶(ALP)、Runt相关转录因子2(Runx2)、骨唾液酸糖蛋白(BSP)、OCN以及破骨相关基因抗酒石酸酸性磷酸酶(TRAP)、活化T细胞核因子(NFATc1)和组织蛋白酶K(CATK)mRNA的表达,TUNEL染色、免疫组化检测骨细胞凋亡情况,蛋白免疫印迹法检测胫骨近端骨组织ERS相关蛋白葡萄糖调节蛋白78(Grp78)、蛋白激酶RNA样内质网激酶(PERK)、磷酸化PERK(p-PERK)、真核翻译起始因子2α(eIF2α)、磷酸化e IF2α(p-eIF2α)、肌醇需求酶1α(IRE1α)、磷酸化IRE1α(p-IRE1α)、激活转录因子6(ATF6)的表达。结果显示,GSQG对去卵巢小鼠血清中OCN、PINP、TRAcP-5b、CTX骨代谢生化指标有明显改善,Micro-CT显示GSQG-H组股骨远端骨微结构明显优于其他组。与模型组比较,HE染色结果显示,GSQG-L组、GSQG-M组、GSQG-H组骨小梁明显变宽,数目增多,网状结构得到了一定程度的恢复,排列仍然整齐,部分间隙轻微增大;TUNEL阳性细胞显著减少(P<0.05,P<0.01);凋亡细胞相关蛋白半胱氨酸天冬氨酸蛋白酶-3(caspase-3)、凋亡因子Bcl-2关联X蛋白(Bax)蛋白表达水平显著降低(P<0.05,P<0.01),Bcl-2蛋白表达显著升高(P<0.05,P<0.01);成骨相关基因Col-Ⅰ、ALP、Runx2、BSP和OCN m RNA表达显著升高(P<0.01),破骨相关基因TRAP、NFATc1和CATK m RNA表达显著减少(P<0.05,P<0.01);ERS相关蛋白Grp78、p-PERK、p-eIF2、p-IRE1α、ATF6表达显著降低(P<0.05,P<0.01),各给药组中以GSQG-H组最为显著。综上研究表明,GSQG能够通过抑制去卵巢小鼠胫骨近端骨组织Grp78/PERK/e IF2α/IRE1α/ATF6信号通路,抑制骨细胞凋亡,从而有效减少去卵巢小鼠骨丢失。展开更多
文摘According to the measured groundwater pollution concentration at two monitoring sections of Gusong River coast,adopting progressive dislocation correlation analysis method,correlation between surface water of Gusong River and groundwater pollution was analyzed.Result showed that propagation time of pollutant between river water and groundwater was longer.According to the water level observation data,river water level was＆gt;1 m higher than groundwater table.The groundwater infiltration parameter was determined through the field permeability test data.The Dupuit formula was used to calculate recharge and discharge relationship between river surface water and groundwater.It was obtained that the daily average recharge capacity of right-bank river water to groundwater was 3 m3,and pollution of river surface water had very small influence on groundwater.Using MATLAB software for data analysis and processing,regression equation was established.The chloride as correlation analysis example,results showed that groundwater chloride had the maximum correlation with observation well distance,and correlation with time and monitoring value of surface water was very small.The linear relationship between surface water and groundwater was not obvious.
文摘Objective: To assess the effect of Gusong II in treating postmenopausal osteoporosis and try to reveal its mechanism. Methods: Thirty-five women, 49 to 62 years old, with 5. 6 years menopause, were treated as the subjects and followed-up for 6 months, 12 months or 24 months. The course of treatment ranged from6 months to 25 months with an average of 14 months. Some related biochemical parameters, such as serumestradiol (E2 ), testosterone (T), the ratio of fasting urinary calcium to creatinine (Ca/Cr), the ratio of 24hurinary calcium to creatinine (24h Ca/Cr), 24h urinary E2, and 24h urinary hydroxyproline (HOP) were measured before and after treatment. Results: After 6～9 months or 1～2 years of treatment, the levels of serum E2and 24h urinary E2 were raised significantly, while the levels of serum T and urinary Ca/Cr fell evidently afterone or two year's treatment. The levels of serum E2 and 24h urinary E2 were significantly correlated with thelevels of fasting urinary Ca/Cr, 24h urinary Ca/Cr, serum T, and 24h urinary HOP respectively. Conclusions:Gusong-II can elevate the serum E2 levels of postmenopausal women, reduce the discharge of urinary calcium andHOP, and inhibit bone resorption. The rise of the serum EZ level might be correlated with the conversion of T toE2.
文摘基于内质网应激(ERS)诱导的细胞凋亡探究骨松强骨方(GSQG)减轻去卵巢小鼠骨丢失的作用与机制。小鼠卵巢摘除(OVX)骨质疏松造模完成后,根据随机数字法,将60只小鼠随机分为6组:假手术组,模型组,骨松强骨方低(GSQG-L)、中(GSQG-M)、高剂量(GSQG-H)组,雌二醇(E2)组,每组10只;切除双侧卵巢构建模型。术后1个月开始灌胃给药,连续给药3个月。酶联免疫吸附测定法(ELISA)测定血清中骨形成指标骨钙素(OCN)、Ⅰ型胶原氨基端前肽(PINP)和骨吸收指标Ⅰ型胶原交联羧基端肽(CTX)、抗酒石酸酸性磷酸酶5b(TRAcP-5b)的水平,使用Micro-CT观察股骨远端骨微结构的改变,HE染色观察骨组织形态,RT-qPCR检测胫骨干成骨相关基因Ⅰ型胶原(Col-Ⅰ)、碱性磷酸酶(ALP)、Runt相关转录因子2(Runx2)、骨唾液酸糖蛋白(BSP)、OCN以及破骨相关基因抗酒石酸酸性磷酸酶(TRAP)、活化T细胞核因子(NFATc1)和组织蛋白酶K(CATK)mRNA的表达,TUNEL染色、免疫组化检测骨细胞凋亡情况,蛋白免疫印迹法检测胫骨近端骨组织ERS相关蛋白葡萄糖调节蛋白78(Grp78)、蛋白激酶RNA样内质网激酶(PERK)、磷酸化PERK(p-PERK)、真核翻译起始因子2α(eIF2α)、磷酸化e IF2α(p-eIF2α)、肌醇需求酶1α(IRE1α)、磷酸化IRE1α(p-IRE1α)、激活转录因子6(ATF6)的表达。结果显示,GSQG对去卵巢小鼠血清中OCN、PINP、TRAcP-5b、CTX骨代谢生化指标有明显改善,Micro-CT显示GSQG-H组股骨远端骨微结构明显优于其他组。与模型组比较,HE染色结果显示,GSQG-L组、GSQG-M组、GSQG-H组骨小梁明显变宽,数目增多,网状结构得到了一定程度的恢复,排列仍然整齐,部分间隙轻微增大;TUNEL阳性细胞显著减少(P<0.05,P<0.01);凋亡细胞相关蛋白半胱氨酸天冬氨酸蛋白酶-3(caspase-3)、凋亡因子Bcl-2关联X蛋白(Bax)蛋白表达水平显著降低(P<0.05,P<0.01),Bcl-2蛋白表达显著升高(P<0.05,P<0.01);成骨相关基因Col-Ⅰ、ALP、Runx2、BSP和OCN m RNA表达显著升高(P<0.01),破骨相关基因TRAP、NFATc1和CATK m RNA表达显著减少(P<0.05,P<0.01);ERS相关蛋白Grp78、p-PERK、p-eIF2、p-IRE1α、ATF6表达显著降低(P<0.05,P<0.01),各给药组中以GSQG-H组最为显著。综上研究表明,GSQG能够通过抑制去卵巢小鼠胫骨近端骨组织Grp78/PERK/e IF2α/IRE1α/ATF6信号通路,抑制骨细胞凋亡,从而有效减少去卵巢小鼠骨丢失。
