BACKGROUND Hepatocellular carcinoma(HCC) is one of the most common malignant tumors worldwide, and novel methods for early/rapid diagnosis of HCC are needed.Terahertz(THz) spectroscopy is considered to have the potent...BACKGROUND Hepatocellular carcinoma(HCC) is one of the most common malignant tumors worldwide, and novel methods for early/rapid diagnosis of HCC are needed.Terahertz(THz) spectroscopy is considered to have the potential to distinguish between normal liver tissue and HCC tissue; however, there are few reports on it.We conduct this observational study to explore the feasibility of THz imaging for the diagnosis of HCC.AIM To evaluate the feasibility of THz for discriminating between HCC and normal liver tissues using fresh tissue specimens obtained from HCC patients who had undergone surgery.METHODS Normal liver tissue and HCC tissue were cryosectioned into 50 μm-thick slicesand placed on cover glass. Two adjacent tissue sections were separated subjected to histopathological examination by hematoxylin and eosin staining or THz transmission examination, and THz images were compared with pathologically mapped images. We determined the typical tumor and normal liver tissue regions by pathological examination; the corresponding areas of adjacent sections were examined by THz transmission.RESULTS The transmission rate of HCC tissue was 0.15-0.25, and the transmission rate of typical HCC tissue was about 0.2. THz transmittance in normal liver tissue is slightly higher than 0.4, but there were many influencing factors, including the degree of liver cirrhosis, fat components, ice crystals in frozen sections, and apoptosis.CONCLUSION In conclusion, this study shows that THz imaging can detect HCC tissue. Further research will yield more detailed data of the THz transmission rates of HCC tissue with different degrees of differentiation.展开更多
目的探讨图像分析技术在病理HE染色质量控制中的应用。方法选择2018年1月至2020年2月广东省惠州市第一妇幼保健院HE染色组织标本60份为研究对象,同一蜡块以3μm厚度连续切片4张,分别染色2、4、6、8min,其他操作程序完全一致。将不同染...目的探讨图像分析技术在病理HE染色质量控制中的应用。方法选择2018年1月至2020年2月广东省惠州市第一妇幼保健院HE染色组织标本60份为研究对象,同一蜡块以3μm厚度连续切片4张,分别染色2、4、6、8min,其他操作程序完全一致。将不同染色时间的4张切片给医生在多头显微镜下共揽,选出标准切片;利用Olympus BX41显微镜及图像采集放大40倍完成一张图片采集,并借助Image Pro Plus软件完成图片细胞核、浆的平均光密度(MOD);采用人工吸管选取法选取10次,比较两种实验方法一致性。结果60张HE染色切片分别采用显微镜观察和图像分析技术干预,结果表明:图像分析技术在消化系统疾病、乳腺及女性生殖系统疾病、皮肤疾病、头颈部疾病、心脏、肺、胸膜及胸腺病病理HE染色与显微镜观察具有高度一致性(P>0.05);两种方法下均完成HE染色,且图像分析技术与人工显微镜观察下细胞核与细胞浆的光密度值无明显差异,获得的图片相对清晰,均无掉片、污染,核仁清晰,整体颜色良好。结论图像分析技术用于病理HE染色中能获得较高的一致性,能获得较高的图像,且该方法在数据保存、诊断、教学中具有明显优势,值得推广应用。展开更多
基金Supported by the National Natural Science Foundation of China,No.11622542 and No.51677145
文摘BACKGROUND Hepatocellular carcinoma(HCC) is one of the most common malignant tumors worldwide, and novel methods for early/rapid diagnosis of HCC are needed.Terahertz(THz) spectroscopy is considered to have the potential to distinguish between normal liver tissue and HCC tissue; however, there are few reports on it.We conduct this observational study to explore the feasibility of THz imaging for the diagnosis of HCC.AIM To evaluate the feasibility of THz for discriminating between HCC and normal liver tissues using fresh tissue specimens obtained from HCC patients who had undergone surgery.METHODS Normal liver tissue and HCC tissue were cryosectioned into 50 μm-thick slicesand placed on cover glass. Two adjacent tissue sections were separated subjected to histopathological examination by hematoxylin and eosin staining or THz transmission examination, and THz images were compared with pathologically mapped images. We determined the typical tumor and normal liver tissue regions by pathological examination; the corresponding areas of adjacent sections were examined by THz transmission.RESULTS The transmission rate of HCC tissue was 0.15-0.25, and the transmission rate of typical HCC tissue was about 0.2. THz transmittance in normal liver tissue is slightly higher than 0.4, but there were many influencing factors, including the degree of liver cirrhosis, fat components, ice crystals in frozen sections, and apoptosis.CONCLUSION In conclusion, this study shows that THz imaging can detect HCC tissue. Further research will yield more detailed data of the THz transmission rates of HCC tissue with different degrees of differentiation.
文摘目的探讨图像分析技术在病理HE染色质量控制中的应用。方法选择2018年1月至2020年2月广东省惠州市第一妇幼保健院HE染色组织标本60份为研究对象,同一蜡块以3μm厚度连续切片4张,分别染色2、4、6、8min,其他操作程序完全一致。将不同染色时间的4张切片给医生在多头显微镜下共揽,选出标准切片;利用Olympus BX41显微镜及图像采集放大40倍完成一张图片采集,并借助Image Pro Plus软件完成图片细胞核、浆的平均光密度(MOD);采用人工吸管选取法选取10次,比较两种实验方法一致性。结果60张HE染色切片分别采用显微镜观察和图像分析技术干预,结果表明:图像分析技术在消化系统疾病、乳腺及女性生殖系统疾病、皮肤疾病、头颈部疾病、心脏、肺、胸膜及胸腺病病理HE染色与显微镜观察具有高度一致性(P>0.05);两种方法下均完成HE染色,且图像分析技术与人工显微镜观察下细胞核与细胞浆的光密度值无明显差异,获得的图片相对清晰,均无掉片、污染,核仁清晰,整体颜色良好。结论图像分析技术用于病理HE染色中能获得较高的一致性,能获得较高的图像,且该方法在数据保存、诊断、教学中具有明显优势,值得推广应用。
