目的:阐明HB s、HBx基因与HBVM的相关性及临床意义。方法:以HCC s、LC ir、SeCH、CHB以及A SC s作为研究对象,应用EL ISA法检测研究对象血清中HB sA g、HB sA b、HB eA g、HB eA b、HB cA b;应用PCR技术、探针杂交法及免疫化学自显影法...目的:阐明HB s、HBx基因与HBVM的相关性及临床意义。方法:以HCC s、LC ir、SeCH、CHB以及A SC s作为研究对象,应用EL ISA法检测研究对象血清中HB sA g、HB sA b、HB eA g、HB eA b、HB cA b;应用PCR技术、探针杂交法及免疫化学自显影法定性检测HB s、HBx基因。结果:在研究对象中,HB sA g+HB eA b+HB cA b、HB sA g+HB eA g+HB cA b和HB sA g+HB cA b模式阳性率分别为:53.3%、27.0%和11.8%;除A SC s组外,各临床类型肝病HB sA g+HB eA b+HB cA b模式阳性率均高于HB sA g+HB eA g+HB cA b模式的阳性率,但差别无统计学意义(P>0.05);与A SC s组比较,HCC s组的HBx基因阳性率明显高于A SC s组(P<0.05),其余各组均无明显差别(P>0.05)。与A SC s组比较,LC ir和SeCH组病人中的HB s基因阳性率显著高于A SC s组(P<0.025),其余各组无明显差异(P>0.05);以HB sA g模式作为对照时,各HBVM模式的HB s、HBx基因阳性率与HB sA g模式的阳性率无明显差别(P>0.05);但在HB sA g+HB eA g+HB cA b模式中阳性率显著高于HB sA g+HB eA b+HB cA b模式的阳性率(P<0.001);HB sA g+HB cA b模式中的HBx基因阳性率也显著高于HB sA g+HB eA b+HB cA b模式的阳性率(P<0.001)。结论:HB sA g+HB eA b+HB cA b模式与肝病的发病关系似乎比HB sA g+HB eA g+HB cA b模式更为密切;HBx基因与HCC s的发生存在密切关系,而HB s基因与LC ir、SeCH的发生关系密切。HB s、HBx基因与HB sA g+HB eA g+HB cA b模式关系密切,而HBx基因还与HB sA g+HB cA b模式存在密切关系。展开更多
The objective of this paper was to analyze the E2 genetic characterization of HB-DCZ strain of Bovine viral diaxrhca Virus （BVDV） which wcrc amplified by RT-PCR and isolated from China. The product of PCK was cloned...The objective of this paper was to analyze the E2 genetic characterization of HB-DCZ strain of Bovine viral diaxrhca Virus （BVDV） which wcrc amplified by RT-PCR and isolated from China. The product of PCK was cloned into pMD18-T vector, and then transfected Escherichia Coli JMI00. The recombinant plasmids were amplified by PCR and were sequenced. From the nucleotide sequence of the amplified products, phylogenetie analyses were performed and genotypes or subgenotypes were identified. The results indicated that the E2 gene fragment of HB-DCZ strain contained 1277bp nucleotides, and had 89.4%, 70.7%, 97.6%, 68.9%, 67.2% sequence similarity with Osloss, OregonC24V, Changchun184, ZM195, NADL, respectively. In conclusion, HB-DCZ strain is closely related to BVDV Osloss, Changchun184, and belongs to subgenotype lb.展开更多
文摘目的:阐明HB s、HBx基因与HBVM的相关性及临床意义。方法:以HCC s、LC ir、SeCH、CHB以及A SC s作为研究对象,应用EL ISA法检测研究对象血清中HB sA g、HB sA b、HB eA g、HB eA b、HB cA b;应用PCR技术、探针杂交法及免疫化学自显影法定性检测HB s、HBx基因。结果:在研究对象中,HB sA g+HB eA b+HB cA b、HB sA g+HB eA g+HB cA b和HB sA g+HB cA b模式阳性率分别为:53.3%、27.0%和11.8%;除A SC s组外,各临床类型肝病HB sA g+HB eA b+HB cA b模式阳性率均高于HB sA g+HB eA g+HB cA b模式的阳性率,但差别无统计学意义(P>0.05);与A SC s组比较,HCC s组的HBx基因阳性率明显高于A SC s组(P<0.05),其余各组均无明显差别(P>0.05)。与A SC s组比较,LC ir和SeCH组病人中的HB s基因阳性率显著高于A SC s组(P<0.025),其余各组无明显差异(P>0.05);以HB sA g模式作为对照时,各HBVM模式的HB s、HBx基因阳性率与HB sA g模式的阳性率无明显差别(P>0.05);但在HB sA g+HB eA g+HB cA b模式中阳性率显著高于HB sA g+HB eA b+HB cA b模式的阳性率(P<0.001);HB sA g+HB cA b模式中的HBx基因阳性率也显著高于HB sA g+HB eA b+HB cA b模式的阳性率(P<0.001)。结论:HB sA g+HB eA b+HB cA b模式与肝病的发病关系似乎比HB sA g+HB eA g+HB cA b模式更为密切;HBx基因与HCC s的发生存在密切关系,而HB s基因与LC ir、SeCH的发生关系密切。HB s、HBx基因与HB sA g+HB eA g+HB cA b模式关系密切,而HBx基因还与HB sA g+HB cA b模式存在密切关系。
文摘The objective of this paper was to analyze the E2 genetic characterization of HB-DCZ strain of Bovine viral diaxrhca Virus （BVDV） which wcrc amplified by RT-PCR and isolated from China. The product of PCK was cloned into pMD18-T vector, and then transfected Escherichia Coli JMI00. The recombinant plasmids were amplified by PCR and were sequenced. From the nucleotide sequence of the amplified products, phylogenetie analyses were performed and genotypes or subgenotypes were identified. The results indicated that the E2 gene fragment of HB-DCZ strain contained 1277bp nucleotides, and had 89.4%, 70.7%, 97.6%, 68.9%, 67.2% sequence similarity with Osloss, OregonC24V, Changchun184, ZM195, NADL, respectively. In conclusion, HB-DCZ strain is closely related to BVDV Osloss, Changchun184, and belongs to subgenotype lb.
