A flexure hinge is a major component in designing compliant mechanisms that o ers unique possibilities in a wide range of application fields in which high positioning accuracy is required. Although various flexure hin...A flexure hinge is a major component in designing compliant mechanisms that o ers unique possibilities in a wide range of application fields in which high positioning accuracy is required. Although various flexure hinges with di erent configurations have been successively proposed, they are often designed based on designers' experiences and inspirations. This study presents a systematic method for topological optimization of flexure hinges by using the level set method. Optimization formulations are developed by considering the functional requirements and geometrical constraints of flexure hinges. The functional requirements are first constructed by maximizing the compliance in the desired direction while minimizing the compliances in the other directions. The weighting sum method is used to construct an objective function in which a self-adjust method is used to set the weighting factors. A constraint on the symmetry of the obtained configuration is developed. Several numerical examples are presented to demonstrate the validity of the proposed method. The obtained results reveal that the design of a flexure hinge starting from the topology level can yield more choices for compliant mechanism design and obtain better designs that achieve higher performance.展开更多
Because of the limited space of the launch rockets, deployable mechanisms are always used to solve the phenomenon. One dimensional deployable mast can deploy and support antenna, solar sail and space optical camera. T...Because of the limited space of the launch rockets, deployable mechanisms are always used to solve the phenomenon. One dimensional deployable mast can deploy and support antenna, solar sail and space optical camera. Tape-spring hyperelastic hinges can be folded and extended into a rod like configuration. It utilizes the strain energy to realize self-deploying and drive the other structures. One kind of triangular prism mast with tape-spring hyperelastic hinges is proposed and developed. Stretching and compression stiffness theoretical model are established with considering the tape-spring hyperelastic hinges based on static theory. The finite element model of ten-module triangular prism mast is set up by ABAQUS with the tape-spring hyperelastic hinge and parameter study is performed to investigate the influence of thickness, section angle and radius. Two-module TPM is processed and tested the compression stiffness by the laser displacement sensor, deploying repeat accuracy by the high speed camera, modal shape and fundamental frequency at cantilever position by LMS multi-channel vibration test and analysis system, which are used to verify precision of the theoretical and finite element models of ten-module triangular prism mast with the tape-spring hyperelastic hinges. This research proposes an innovative one dimensional triangular prism with tape-spring hyperelastic hinge which has great application value to the space deployable mechanisms.展开更多
Based on the elastic foundation beam theory and the multi-floating-module hydrodynamic theory,a novel method is proposed to estimate the dynamic responses of VLFS(Very Large Floating Structure).In still water,a VLFS c...Based on the elastic foundation beam theory and the multi-floating-module hydrodynamic theory,a novel method is proposed to estimate the dynamic responses of VLFS(Very Large Floating Structure).In still water,a VLFS can be simplified as an elastic foundation beam model or a multi-floating-module model connected by elastic hinges.According to equivalent displacement of the two models in static analysis,the problem of rotation stiffness of elastic hinges can be solved.Then,based on the potential flow theory,the dynamic responding analysis of multi-floatingmodule model under wave loads can be computed in ANSYS-AQWA software.By assembling the time domain analysis results of each module,the dynamic responses of the VLFS can be obtained.Validation of the method is conducted through a series of comparison calculations,which mainly includes a continuous structure and a three-part structure connected by hinges in regular waves.The results of this paper method show a satisfactory agreement with the experiment and calculation data given in relative references.展开更多
This paper presents the shear performance analysis of a heavy-duty universal hinged cast steel support with the largest bearing capacity. The effect of 9 parameters ( 52 specimens) ,i. e. height of the upper support,d...This paper presents the shear performance analysis of a heavy-duty universal hinged cast steel support with the largest bearing capacity. The effect of 9 parameters ( 52 specimens) ,i. e. height of the upper support,depth of the ring of the upper support,depth of the top plate of the bottom support,height of the ribs of the bottom support,depth of the ribs of the bottom support,bolt hole diameter,number of the ribs of the bowl,depth of the ribs of the bowl,and yield strength of the material,were analyzed with a 3-dimensional elastic-plastic finite element model in which the nonlinearities of geometry,material and contact were all considered. Analysis shows that height of the upper support,depth of the ring of the upper support and yield strength of the material have a great effect on the mechanical performance of the support. Height of the upper support has the largest effect on performance price ratio of the support,and the maximum effect can be up to 160% . Depth of the top plate of the bottom support,height of the ribs of the bottom support and depth of the ribs of the bottom support have a medium effect on performance price ratio of the support,and the effect is within the limit of 15% 19% .展开更多
The accuracy and effi ciency of the modelling techniques utilized to model the nonlinear behavior of structural components is a signifi cant issue in earthquake engineering. In this study, the suffi ciency of three di...The accuracy and effi ciency of the modelling techniques utilized to model the nonlinear behavior of structural components is a signifi cant issue in earthquake engineering. In this study, the suffi ciency of three diff erent modelling techniques that can be employed to simulate the structural behavior of columns is investigated. A fi ber-based fi nite length plastic hinge (FB-FLPH) model is calibrated in this study. In order to calibrate the FB-FLPH model, a novel database of the cyclic behavior of hollow steel columns under simultaneous axial and lateral loading cycles with varying amplitudes is used. By employing the FB-FLPH model calibrated in this study, the interaction of the axial force and the bending moment in columns is directly taken into account, and the deterioration in the cyclic behavior of these members is implicitly considered. The superiority of the calibrated FB-FLPH modelling approach is examined compared with the cases in which conventional fi ber-based distributed plasticity and concentrated plasticity models are utilized. The effi ciency of the enumerated modelling techniques is probed when they are implemented to model the columns of a typical special moment frame in order to prove the advantage of the FB-FLPH modelling approach.展开更多
The purpose of this thesis is to derive the flexibility formula of the corner-filleted flexure hinge easily and conveniently and use it to design a micro-rotation compliant mechanism. Firstly,we get the corner-fillete...The purpose of this thesis is to derive the flexibility formula of the corner-filleted flexure hinge easily and conveniently and use it to design a micro-rotation compliant mechanism. Firstly,we get the corner-filleted flexure hinge flexibility formula by methods of symmetry transformation and coordinates translation. The correctness of this formula is validated on the basis of the finite element method and under the premise that the effects of shear stress are taken into consideration. Then a micro-rotation compliant mechanism is designed in accordance with the corner-filleted flexure hinge,and the deduction and analysis of its working moment/rigidity are conducted. Moreover,this theoretical formula is proved to be accurate and reliable through the finite element analysis and the experimental verification,based on which the structural design and optimization can be made on the rotating part of a micro adjustment device. The results illustrate that designing and optimizing the structures by the analysis model is convenient and reliable so that complicated 3D modeling and finite element analysis are not needed.展开更多
In this paper,the theorem of structure continual variation of truss structure in the analysis of structure reliability is derived,and it is used to generate limit state function automatically.We can avoid repeated ass...In this paper,the theorem of structure continual variation of truss structure in the analysis of structure reliability is derived,and it is used to generate limit state function automatically.We can avoid repeated assembly of global stiffness matrix and repeated inverse operations of the matrix caused by constant changes of structure topology.A new criterion of degenerate of the structure into mechanism is introduced.The calculation examples are satisfactory.展开更多
The uncertainty widely exists in the engineering practice.Therefore,it is necessary to research the effect of uncertainty on the structural system. In this paper,the reliability and sensitivity of the flexure hinge, w...The uncertainty widely exists in the engineering practice.Therefore,it is necessary to research the effect of uncertainty on the structural system. In this paper,the reliability and sensitivity of the flexure hinge, which is the key component of the compliant mechanisms,are investigated. The results of the reliability analysis can effectively guide the engineer to design and optimize the flexure hinge. In order to improve the calculating efficiency,the kriging method is introduced to estimate the failure probability and reliability sensitivity.展开更多
This paper presents an in-depth study of Equivalent beam model (EBM).Firstly three-dimensional (3D) finite element analysis (FEA) model for circular flexure hinge developed by Zettl et al.was verified by the compariso...This paper presents an in-depth study of Equivalent beam model (EBM).Firstly three-dimensional (3D) finite element analysis (FEA) model for circular flexure hinge developed by Zettl et al.was verified by the comparison with Smith's experimental results and the 3D FEA model was feasible within 5.5% error.Then the accuracy of Timoshenko short-beam due to shear force was verified based on finite element method.The results showed that the EBM has good accuracy within 5% error for 1≤r/t≤3.Finally the EBM methodology was applied for the simulation optimal design of a bridge-type compliant mechanism.The results showed that the EBM methodology has very high numerical efficiency and satisfactory accuracy for simulation optimal design of planar compliant mechanism with flexure hinges.展开更多
Study of dynamic stability phenomenon in transient systems has always created interest amongst the researchers because of its inherent non-linearities. Offshore structures subjected to wave, earthquake or wind loads o...Study of dynamic stability phenomenon in transient systems has always created interest amongst the researchers because of its inherent non-linearities. Offshore structures subjected to wave, earthquake or wind loads or a combination of these loads show non-linear transient behaviour. As oceanic waves are better modelled as stochastic process, there is a need to investigate the stochastic stability of flexible offshore structures as well. Present study has been carried out to determine seismic response of Single Hinged Articulated Tower (SHAT) under different categories of wave loads and earthquake followed by its dynamic stability analysis. Different phases of wave/earthquake loading on SHAT have been explored to investigate dynamic instabilities existing during each phase. Two dimensional phase plots have been used to identify phases of dynamic instability existing within the responses of SHAT under various conditions of loading.展开更多
Dirac semimetals(DSMs)are an important class of topological states of matter.Here,focusing on DSMs of band inversion type,we investigate their boundary modes from the effective model perspective.We show that in order ...Dirac semimetals(DSMs)are an important class of topological states of matter.Here,focusing on DSMs of band inversion type,we investigate their boundary modes from the effective model perspective.We show that in order to properly capture the boundary modes,k-cubic terms must be included in the effective model,which would drive an evolution of surface degeneracy manifold from a nodal line to a nodal point.Sizable k-cubic terms are also needed for better exposing the topological hinge modes in the spectrum.Using first-principles calculations,we demonstrate that this feature and the topological hinge modes can be clearly exhibited inβ-CuI.We extend the discussion to magnetic DSMs and show that the time-reversal symmetry breaking can gap out the surface bands and hence is beneficial for the experimental detection of hinge modes.Furthermore,we show that magnetic DSMs serve as a parent state for realizing multiple other higher-order topological phases,including higher-order Weyl-point/nodal-line semimetals and higher-order topological insulators.展开更多
In this study, the influence of confined concrete models on the response of reinforced concrete structures is investigatedat member and global system levels. The commonly encountered concrete models such as Modified K...In this study, the influence of confined concrete models on the response of reinforced concrete structures is investigatedat member and global system levels. The commonly encountered concrete models such as Modified Kent-Park, Saatçioğlu-Razvi, and Mander are considered. Two moment-resisting frames designed according to thepre-modern code are taken into consideration to reflect the example of an RC moment-resisting frame in thecurrent building stock. The building is in an earthquake-prone zone located on Z3 Soil Type. The inelasticresponse of the building frame is modelled by considering the plastic hinges formed on each beam and columnelement for different concrete classes and stirrups spacings. The models are subjected to non-linear static analyses.The differences between confined concrete models are comparatively investigated at both reinforced concretemember and system levels. Based on the results of the comparative analysis, it is revealed that the column behaviouris mostly influenced by the choice of model, due to axial loads and confinement effects, while the beams areless affected, and also it is observed that the differences exhibited in the moment-curvature response of columncross-sections do not significantly affect the overall behaviour of the global system. This highlights the critical roleof model selection relative to the concrete strength and stirrup spacing of the member.展开更多
The coordinated utilization of nitrogen(N)and phosphorus(P)is vital for plants to maintain nutrient balance and achieve optimal growth.Previously,we revealed a mechanism by which nitrate induces genes for phosphate ut...The coordinated utilization of nitrogen(N)and phosphorus(P)is vital for plants to maintain nutrient balance and achieve optimal growth.Previously,we revealed a mechanism by which nitrate induces genes for phosphate utilization;this mechanism depends on NRT1.1B-facilitated degradation of cytoplasmic SPX4,which in turn promotes cytoplasmic-nuclear shuttling of PHR2,the central transcription factor of phosphate signaling,and triggers the nitrate-induced phosphate response(NIPR)and N-P coordinated utilization in rice.In this study,we unveiled a fine-tuning mechanism of NIPR in the nucleus regulated by Highly Induced by Nitrate Gene 1(HINGE1,also known as RLI1),a MYB-transcription factor closely related to PHR2.RLI1/HINGE1,which is transcriptionally activated by PHR2 under nitrate induction,can directly activate the expression of phosphate starvation-induced genes.More importantly,RLI1/HINGE1 competes with PHR2 for binding to its repressor proteins in the nucleus(SPX proteins),and consequently releases PHR2 to further enhance phosphate response.Therefore,RLI1/HINGE1 amplifies the phosphate response in the nucleus downstream of the cytoplasmic SPX4-PHR2 cascade,thereby enabling fine-tuning of N-P balance when nitrate supply is sufficient.展开更多
This paper presents a new robust topology optimization framework for hinge-free compliant mechanisms with spatially varying material uncertainties,which are described using a non-probabilistic bounded field model.Boun...This paper presents a new robust topology optimization framework for hinge-free compliant mechanisms with spatially varying material uncertainties,which are described using a non-probabilistic bounded field model.Bounded field uncertainties are efficiently represented by a reduced set of uncertain-but-bouncled coeflficients on the basis of the series expansion method.Robust topology optimization of compliant mechanisms is then defined to minimize the variation in output displacement under constraints of the mean displacement and predefined material volume.The nest optimization problem is solved using a gradient-based optimization algorithm.Numerical examples are presented to illustrate the effectiveness of the proposed method for circumventing hinges in topology optimization of compliant mechanisms.展开更多
基金Supported by National Natural Science Foundation of China(Grant Nos.51605166,51820105007)Fundamental Research Funds for the Central Universities of China
文摘A flexure hinge is a major component in designing compliant mechanisms that o ers unique possibilities in a wide range of application fields in which high positioning accuracy is required. Although various flexure hinges with di erent configurations have been successively proposed, they are often designed based on designers' experiences and inspirations. This study presents a systematic method for topological optimization of flexure hinges by using the level set method. Optimization formulations are developed by considering the functional requirements and geometrical constraints of flexure hinges. The functional requirements are first constructed by maximizing the compliance in the desired direction while minimizing the compliances in the other directions. The weighting sum method is used to construct an objective function in which a self-adjust method is used to set the weighting factors. A constraint on the symmetry of the obtained configuration is developed. Several numerical examples are presented to demonstrate the validity of the proposed method. The obtained results reveal that the design of a flexure hinge starting from the topology level can yield more choices for compliant mechanism design and obtain better designs that achieve higher performance.
基金Supported by National Natural Science Foundation of China(Grant No.51605001)Joint Funds of the National Natural Science Foundation of China(Grant No.U1637207)Anhui University Research Foundation for Doctor(Grant No.J01003222)
文摘Because of the limited space of the launch rockets, deployable mechanisms are always used to solve the phenomenon. One dimensional deployable mast can deploy and support antenna, solar sail and space optical camera. Tape-spring hyperelastic hinges can be folded and extended into a rod like configuration. It utilizes the strain energy to realize self-deploying and drive the other structures. One kind of triangular prism mast with tape-spring hyperelastic hinges is proposed and developed. Stretching and compression stiffness theoretical model are established with considering the tape-spring hyperelastic hinges based on static theory. The finite element model of ten-module triangular prism mast is set up by ABAQUS with the tape-spring hyperelastic hinge and parameter study is performed to investigate the influence of thickness, section angle and radius. Two-module TPM is processed and tested the compression stiffness by the laser displacement sensor, deploying repeat accuracy by the high speed camera, modal shape and fundamental frequency at cantilever position by LMS multi-channel vibration test and analysis system, which are used to verify precision of the theoretical and finite element models of ten-module triangular prism mast with the tape-spring hyperelastic hinges. This research proposes an innovative one dimensional triangular prism with tape-spring hyperelastic hinge which has great application value to the space deployable mechanisms.
基金financially supported by the High-Tech Ship Research Projects sponsored by the Ministry of Industry and Information Technology of China(Grant No.[2019]357)China Postdoctoral Science Foundation(Grant No.2020M683755)。
文摘Based on the elastic foundation beam theory and the multi-floating-module hydrodynamic theory,a novel method is proposed to estimate the dynamic responses of VLFS(Very Large Floating Structure).In still water,a VLFS can be simplified as an elastic foundation beam model or a multi-floating-module model connected by elastic hinges.According to equivalent displacement of the two models in static analysis,the problem of rotation stiffness of elastic hinges can be solved.Then,based on the potential flow theory,the dynamic responding analysis of multi-floatingmodule model under wave loads can be computed in ANSYS-AQWA software.By assembling the time domain analysis results of each module,the dynamic responses of the VLFS can be obtained.Validation of the method is conducted through a series of comparison calculations,which mainly includes a continuous structure and a three-part structure connected by hinges in regular waves.The results of this paper method show a satisfactory agreement with the experiment and calculation data given in relative references.
基金Sponsored by the National Natural Science Foundation of China( Grant No. 50878066)the National Key Technology R&D Program during the 11th Five-Year Plan Period of China( Grant No. 2006BAJ01B02)
文摘This paper presents the shear performance analysis of a heavy-duty universal hinged cast steel support with the largest bearing capacity. The effect of 9 parameters ( 52 specimens) ,i. e. height of the upper support,depth of the ring of the upper support,depth of the top plate of the bottom support,height of the ribs of the bottom support,depth of the ribs of the bottom support,bolt hole diameter,number of the ribs of the bowl,depth of the ribs of the bowl,and yield strength of the material,were analyzed with a 3-dimensional elastic-plastic finite element model in which the nonlinearities of geometry,material and contact were all considered. Analysis shows that height of the upper support,depth of the ring of the upper support and yield strength of the material have a great effect on the mechanical performance of the support. Height of the upper support has the largest effect on performance price ratio of the support,and the maximum effect can be up to 160% . Depth of the top plate of the bottom support,height of the ribs of the bottom support and depth of the ribs of the bottom support have a medium effect on performance price ratio of the support,and the effect is within the limit of 15% 19% .
文摘The accuracy and effi ciency of the modelling techniques utilized to model the nonlinear behavior of structural components is a signifi cant issue in earthquake engineering. In this study, the suffi ciency of three diff erent modelling techniques that can be employed to simulate the structural behavior of columns is investigated. A fi ber-based fi nite length plastic hinge (FB-FLPH) model is calibrated in this study. In order to calibrate the FB-FLPH model, a novel database of the cyclic behavior of hollow steel columns under simultaneous axial and lateral loading cycles with varying amplitudes is used. By employing the FB-FLPH model calibrated in this study, the interaction of the axial force and the bending moment in columns is directly taken into account, and the deterioration in the cyclic behavior of these members is implicitly considered. The superiority of the calibrated FB-FLPH modelling approach is examined compared with the cases in which conventional fi ber-based distributed plasticity and concentrated plasticity models are utilized. The effi ciency of the enumerated modelling techniques is probed when they are implemented to model the columns of a typical special moment frame in order to prove the advantage of the FB-FLPH modelling approach.
基金Sponsored by the National High-tech R&D Progrom(Grant No.2011AA12A103)the Equipment Development Fund(Grant No.08001SA050)
文摘The purpose of this thesis is to derive the flexibility formula of the corner-filleted flexure hinge easily and conveniently and use it to design a micro-rotation compliant mechanism. Firstly,we get the corner-filleted flexure hinge flexibility formula by methods of symmetry transformation and coordinates translation. The correctness of this formula is validated on the basis of the finite element method and under the premise that the effects of shear stress are taken into consideration. Then a micro-rotation compliant mechanism is designed in accordance with the corner-filleted flexure hinge,and the deduction and analysis of its working moment/rigidity are conducted. Moreover,this theoretical formula is proved to be accurate and reliable through the finite element analysis and the experimental verification,based on which the structural design and optimization can be made on the rotating part of a micro adjustment device. The results illustrate that designing and optimizing the structures by the analysis model is convenient and reliable so that complicated 3D modeling and finite element analysis are not needed.
文摘In this paper,the theorem of structure continual variation of truss structure in the analysis of structure reliability is derived,and it is used to generate limit state function automatically.We can avoid repeated assembly of global stiffness matrix and repeated inverse operations of the matrix caused by constant changes of structure topology.A new criterion of degenerate of the structure into mechanism is introduced.The calculation examples are satisfactory.
基金Foundations of China Academic Engineering Physics(CAEP)(Nos.2013B0203028,2014B0203023)Technology Foundation Project,China(No.2015ZK1.1)
文摘The uncertainty widely exists in the engineering practice.Therefore,it is necessary to research the effect of uncertainty on the structural system. In this paper,the reliability and sensitivity of the flexure hinge, which is the key component of the compliant mechanisms,are investigated. The results of the reliability analysis can effectively guide the engineer to design and optimize the flexure hinge. In order to improve the calculating efficiency,the kriging method is introduced to estimate the failure probability and reliability sensitivity.
文摘This paper presents an in-depth study of Equivalent beam model (EBM).Firstly three-dimensional (3D) finite element analysis (FEA) model for circular flexure hinge developed by Zettl et al.was verified by the comparison with Smith's experimental results and the 3D FEA model was feasible within 5.5% error.Then the accuracy of Timoshenko short-beam due to shear force was verified based on finite element method.The results showed that the EBM has good accuracy within 5% error for 1≤r/t≤3.Finally the EBM methodology was applied for the simulation optimal design of a bridge-type compliant mechanism.The results showed that the EBM methodology has very high numerical efficiency and satisfactory accuracy for simulation optimal design of planar compliant mechanism with flexure hinges.
文摘Study of dynamic stability phenomenon in transient systems has always created interest amongst the researchers because of its inherent non-linearities. Offshore structures subjected to wave, earthquake or wind loads or a combination of these loads show non-linear transient behaviour. As oceanic waves are better modelled as stochastic process, there is a need to investigate the stochastic stability of flexible offshore structures as well. Present study has been carried out to determine seismic response of Single Hinged Articulated Tower (SHAT) under different categories of wave loads and earthquake followed by its dynamic stability analysis. Different phases of wave/earthquake loading on SHAT have been explored to investigate dynamic instabilities existing during each phase. Two dimensional phase plots have been used to identify phases of dynamic instability existing within the responses of SHAT under various conditions of loading.
文摘Dirac semimetals(DSMs)are an important class of topological states of matter.Here,focusing on DSMs of band inversion type,we investigate their boundary modes from the effective model perspective.We show that in order to properly capture the boundary modes,k-cubic terms must be included in the effective model,which would drive an evolution of surface degeneracy manifold from a nodal line to a nodal point.Sizable k-cubic terms are also needed for better exposing the topological hinge modes in the spectrum.Using first-principles calculations,we demonstrate that this feature and the topological hinge modes can be clearly exhibited inβ-CuI.We extend the discussion to magnetic DSMs and show that the time-reversal symmetry breaking can gap out the surface bands and hence is beneficial for the experimental detection of hinge modes.Furthermore,we show that magnetic DSMs serve as a parent state for realizing multiple other higher-order topological phases,including higher-order Weyl-point/nodal-line semimetals and higher-order topological insulators.
文摘In this study, the influence of confined concrete models on the response of reinforced concrete structures is investigatedat member and global system levels. The commonly encountered concrete models such as Modified Kent-Park, Saatçioğlu-Razvi, and Mander are considered. Two moment-resisting frames designed according to thepre-modern code are taken into consideration to reflect the example of an RC moment-resisting frame in thecurrent building stock. The building is in an earthquake-prone zone located on Z3 Soil Type. The inelasticresponse of the building frame is modelled by considering the plastic hinges formed on each beam and columnelement for different concrete classes and stirrups spacings. The models are subjected to non-linear static analyses.The differences between confined concrete models are comparatively investigated at both reinforced concretemember and system levels. Based on the results of the comparative analysis, it is revealed that the column behaviouris mostly influenced by the choice of model, due to axial loads and confinement effects, while the beams areless affected, and also it is observed that the differences exhibited in the moment-curvature response of columncross-sections do not significantly affect the overall behaviour of the global system. This highlights the critical roleof model selection relative to the concrete strength and stirrup spacing of the member.
基金This work was supported by the National Key Research and Development Program of China(2016YFD0101801,2009CB118506)the National Natural Sciences Foundation of China(31771348,32002119)China Postdoctoral Science Foundation(2020M672569).
文摘The coordinated utilization of nitrogen(N)and phosphorus(P)is vital for plants to maintain nutrient balance and achieve optimal growth.Previously,we revealed a mechanism by which nitrate induces genes for phosphate utilization;this mechanism depends on NRT1.1B-facilitated degradation of cytoplasmic SPX4,which in turn promotes cytoplasmic-nuclear shuttling of PHR2,the central transcription factor of phosphate signaling,and triggers the nitrate-induced phosphate response(NIPR)and N-P coordinated utilization in rice.In this study,we unveiled a fine-tuning mechanism of NIPR in the nucleus regulated by Highly Induced by Nitrate Gene 1(HINGE1,also known as RLI1),a MYB-transcription factor closely related to PHR2.RLI1/HINGE1,which is transcriptionally activated by PHR2 under nitrate induction,can directly activate the expression of phosphate starvation-induced genes.More importantly,RLI1/HINGE1 competes with PHR2 for binding to its repressor proteins in the nucleus(SPX proteins),and consequently releases PHR2 to further enhance phosphate response.Therefore,RLI1/HINGE1 amplifies the phosphate response in the nucleus downstream of the cytoplasmic SPX4-PHR2 cascade,thereby enabling fine-tuning of N-P balance when nitrate supply is sufficient.
基金the National Key R&D Program of China(Grant No.2017YFB0203604)the National Natural Science Foundation of China(Grant No.11472215).
文摘This paper presents a new robust topology optimization framework for hinge-free compliant mechanisms with spatially varying material uncertainties,which are described using a non-probabilistic bounded field model.Bounded field uncertainties are efficiently represented by a reduced set of uncertain-but-bouncled coeflficients on the basis of the series expansion method.Robust topology optimization of compliant mechanisms is then defined to minimize the variation in output displacement under constraints of the mean displacement and predefined material volume.The nest optimization problem is solved using a gradient-based optimization algorithm.Numerical examples are presented to illustrate the effectiveness of the proposed method for circumventing hinges in topology optimization of compliant mechanisms.